Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina

Autores
Corigliano, Mariana Georgina; de Guzmán, Ana María Stefanini; Stagnitta, Patricia Virginia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production.The aim of this work was: i) to assess the sanitary-hygienic quality of dehydrated soups (100 samples) consumed in San Luis – Argentina; ii) to verify the presence of C. perfringens in these food products using the "Most Probable Number" method (MPN) and plate-counting methods; iii) to characterise enterotoxigenicity in strain isolates by RPLA; iv) to determine the chromosomal or plasmidic location of the cpe gene in enterotoxigenic strains previously isolated from food in our lab, using PCR; v) to correlate chromosomal cpe and spore heat-resistance; vi) to compare protease activity in cpe+ and cpe– strains; and vii) to compare azoreductase activity in cpe+ and cpe– strains. Twenty-six isolates had a count a 3–43 bacteria g-1 count using MPN; 7.7% exceeded the Argentine Food Code (CAA) limit. All isolates showed protease activity: enterotoxigenic isolates had higher protease activity than non-enterotoxigenic isolates. All isolates showed azoreductase activity: enterotoxigenic isolates had higher activity and shorter reducing times. Enterotoxigenic isolates showed chromosomal location for the gene responsible for the enterotoxin.
Fil: Corigliano, Mariana Georgina. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Guzmán, Ana María Stefanini. Universidad Nacional de San Luis; Argentina
Fil: Stagnitta, Patricia Virginia. Universidad Nacional de San Luis; Argentina
Materia
ENTEROTOXIN
PROTEASE
AZOREDUCTASE
food
clostridium perfringens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102555
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - ArgentinaCorigliano, Mariana Georginade Guzmán, Ana María StefaniniStagnitta, Patricia VirginiaENTEROTOXINPROTEASEAZOREDUCTASEfoodclostridium perfringenshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production.The aim of this work was: i) to assess the sanitary-hygienic quality of dehydrated soups (100 samples) consumed in San Luis – Argentina; ii) to verify the presence of C. perfringens in these food products using the "Most Probable Number" method (MPN) and plate-counting methods; iii) to characterise enterotoxigenicity in strain isolates by RPLA; iv) to determine the chromosomal or plasmidic location of the cpe gene in enterotoxigenic strains previously isolated from food in our lab, using PCR; v) to correlate chromosomal cpe and spore heat-resistance; vi) to compare protease activity in cpe+ and cpe– strains; and vii) to compare azoreductase activity in cpe+ and cpe– strains. Twenty-six isolates had a count a 3–43 bacteria g-1 count using MPN; 7.7% exceeded the Argentine Food Code (CAA) limit. All isolates showed protease activity: enterotoxigenic isolates had higher protease activity than non-enterotoxigenic isolates. All isolates showed azoreductase activity: enterotoxigenic isolates had higher activity and shorter reducing times. Enterotoxigenic isolates showed chromosomal location for the gene responsible for the enterotoxin.Fil: Corigliano, Mariana Georgina. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: de Guzmán, Ana María Stefanini. Universidad Nacional de San Luis; ArgentinaFil: Stagnitta, Patricia Virginia. Universidad Nacional de San Luis; ArgentinaNational Institute of Public Health2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102555Corigliano, Mariana Georgina; de Guzmán, Ana María Stefanini; Stagnitta, Patricia Virginia; Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina; National Institute of Public Health; Central European Journal of Public Health; 19; 1; 3-2011; 46-531803-1048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www1.szu.cz/svi/cejph/show.php?kat=archiv/2011-1-09info:eu-repo/semantics/altIdentifier/doi/10.21101/cejph.a3597info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:01Zoai:ri.conicet.gov.ar:11336/102555instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:02.073CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
title Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
spellingShingle Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
Corigliano, Mariana Georgina
ENTEROTOXIN
PROTEASE
AZOREDUCTASE
food
clostridium perfringens
title_short Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
title_full Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
title_fullStr Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
title_full_unstemmed Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
title_sort Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina
dc.creator.none.fl_str_mv Corigliano, Mariana Georgina
de Guzmán, Ana María Stefanini
Stagnitta, Patricia Virginia
author Corigliano, Mariana Georgina
author_facet Corigliano, Mariana Georgina
de Guzmán, Ana María Stefanini
Stagnitta, Patricia Virginia
author_role author
author2 de Guzmán, Ana María Stefanini
Stagnitta, Patricia Virginia
author2_role author
author
dc.subject.none.fl_str_mv ENTEROTOXIN
PROTEASE
AZOREDUCTASE
food
clostridium perfringens
topic ENTEROTOXIN
PROTEASE
AZOREDUCTASE
food
clostridium perfringens
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production.The aim of this work was: i) to assess the sanitary-hygienic quality of dehydrated soups (100 samples) consumed in San Luis – Argentina; ii) to verify the presence of C. perfringens in these food products using the "Most Probable Number" method (MPN) and plate-counting methods; iii) to characterise enterotoxigenicity in strain isolates by RPLA; iv) to determine the chromosomal or plasmidic location of the cpe gene in enterotoxigenic strains previously isolated from food in our lab, using PCR; v) to correlate chromosomal cpe and spore heat-resistance; vi) to compare protease activity in cpe+ and cpe– strains; and vii) to compare azoreductase activity in cpe+ and cpe– strains. Twenty-six isolates had a count a 3–43 bacteria g-1 count using MPN; 7.7% exceeded the Argentine Food Code (CAA) limit. All isolates showed protease activity: enterotoxigenic isolates had higher protease activity than non-enterotoxigenic isolates. All isolates showed azoreductase activity: enterotoxigenic isolates had higher activity and shorter reducing times. Enterotoxigenic isolates showed chromosomal location for the gene responsible for the enterotoxin.
Fil: Corigliano, Mariana Georgina. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: de Guzmán, Ana María Stefanini. Universidad Nacional de San Luis; Argentina
Fil: Stagnitta, Patricia Virginia. Universidad Nacional de San Luis; Argentina
description Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production.The aim of this work was: i) to assess the sanitary-hygienic quality of dehydrated soups (100 samples) consumed in San Luis – Argentina; ii) to verify the presence of C. perfringens in these food products using the "Most Probable Number" method (MPN) and plate-counting methods; iii) to characterise enterotoxigenicity in strain isolates by RPLA; iv) to determine the chromosomal or plasmidic location of the cpe gene in enterotoxigenic strains previously isolated from food in our lab, using PCR; v) to correlate chromosomal cpe and spore heat-resistance; vi) to compare protease activity in cpe+ and cpe– strains; and vii) to compare azoreductase activity in cpe+ and cpe– strains. Twenty-six isolates had a count a 3–43 bacteria g-1 count using MPN; 7.7% exceeded the Argentine Food Code (CAA) limit. All isolates showed protease activity: enterotoxigenic isolates had higher protease activity than non-enterotoxigenic isolates. All isolates showed azoreductase activity: enterotoxigenic isolates had higher activity and shorter reducing times. Enterotoxigenic isolates showed chromosomal location for the gene responsible for the enterotoxin.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102555
Corigliano, Mariana Georgina; de Guzmán, Ana María Stefanini; Stagnitta, Patricia Virginia; Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina; National Institute of Public Health; Central European Journal of Public Health; 19; 1; 3-2011; 46-53
1803-1048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102555
identifier_str_mv Corigliano, Mariana Georgina; de Guzmán, Ana María Stefanini; Stagnitta, Patricia Virginia; Characterization of the Plasmidic or Chromosomal cpe Gene and Metabolic Activities in Clostridium perfringens Isolates from Food in San Luis - Argentina; National Institute of Public Health; Central European Journal of Public Health; 19; 1; 3-2011; 46-53
1803-1048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www1.szu.cz/svi/cejph/show.php?kat=archiv/2011-1-09
info:eu-repo/semantics/altIdentifier/doi/10.21101/cejph.a3597
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Institute of Public Health
publisher.none.fl_str_mv National Institute of Public Health
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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