CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela

Autores
Millan, Beatriz; Ghiglione, Barbara; Diaz, Tulia; Gutkind, Gabriel Osvaldo; Araque, María
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A clinical isolate of C. freundii with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the blaCTX-M-14 gene in the pLM07/20 from C. freundii.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.
Fil: Millan, Beatriz. Universidad de Los Andes; Venezuela
Fil: Ghiglione, Barbara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Diaz, Tulia. Universidad de Los Andes; Venezuela
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Araque, María. Universidad de Los Andes; Venezuela
Materia
Citrobacter freundi
CTX-M-14,
PLASMID
FrepB REPLICON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12924
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12924
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in VenezuelaMillan, BeatrizGhiglione, BarbaraDiaz, TuliaGutkind, Gabriel OsvaldoAraque, MaríaCitrobacter freundiCTX-M-14,PLASMIDFrepB REPLICONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3A clinical isolate of C. freundii with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the blaCTX-M-14 gene in the pLM07/20 from C. freundii.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.Fil: Millan, Beatriz. Universidad de Los Andes; VenezuelaFil: Ghiglione, Barbara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Diaz, Tulia. Universidad de Los Andes; VenezuelaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Araque, María. Universidad de Los Andes; VenezuelaBioMed Central2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12924Millan, Beatriz; Ghiglione, Barbara; Diaz, Tulia; Gutkind, Gabriel Osvaldo; Araque, María; CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela; BioMed Central; Annals of Clinical Microbiology and Antimicrobials; 10; 22; 5-2011; 1-41476-0711enginfo:eu-repo/semantics/altIdentifier/url/http://ann-clinmicrob.biomedcentral.com/articles/10.1186/1476-0711-10-22info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121586/info:eu-repo/semantics/altIdentifier/doi/10.1186/1476-0711-10-22info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:34Zoai:ri.conicet.gov.ar:11336/12924instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:34.788CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
title CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
spellingShingle CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
Millan, Beatriz
Citrobacter freundi
CTX-M-14,
PLASMID
FrepB REPLICON
title_short CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
title_full CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
title_fullStr CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
title_full_unstemmed CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
title_sort CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela
dc.creator.none.fl_str_mv Millan, Beatriz
Ghiglione, Barbara
Diaz, Tulia
Gutkind, Gabriel Osvaldo
Araque, María
author Millan, Beatriz
author_facet Millan, Beatriz
Ghiglione, Barbara
Diaz, Tulia
Gutkind, Gabriel Osvaldo
Araque, María
author_role author
author2 Ghiglione, Barbara
Diaz, Tulia
Gutkind, Gabriel Osvaldo
Araque, María
author2_role author
author
author
author
dc.subject.none.fl_str_mv Citrobacter freundi
CTX-M-14,
PLASMID
FrepB REPLICON
topic Citrobacter freundi
CTX-M-14,
PLASMID
FrepB REPLICON
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A clinical isolate of C. freundii with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the blaCTX-M-14 gene in the pLM07/20 from C. freundii.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.
Fil: Millan, Beatriz. Universidad de Los Andes; Venezuela
Fil: Ghiglione, Barbara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Diaz, Tulia. Universidad de Los Andes; Venezuela
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Araque, María. Universidad de Los Andes; Venezuela
description A clinical isolate of C. freundii with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the blaCTX-M-14 gene in the pLM07/20 from C. freundii.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12924
Millan, Beatriz; Ghiglione, Barbara; Diaz, Tulia; Gutkind, Gabriel Osvaldo; Araque, María; CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela; BioMed Central; Annals of Clinical Microbiology and Antimicrobials; 10; 22; 5-2011; 1-4
1476-0711
url http://hdl.handle.net/11336/12924
identifier_str_mv Millan, Beatriz; Ghiglione, Barbara; Diaz, Tulia; Gutkind, Gabriel Osvaldo; Araque, María; CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela; BioMed Central; Annals of Clinical Microbiology and Antimicrobials; 10; 22; 5-2011; 1-4
1476-0711
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ann-clinmicrob.biomedcentral.com/articles/10.1186/1476-0711-10-22
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3121586/
info:eu-repo/semantics/altIdentifier/doi/10.1186/1476-0711-10-22
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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