Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo

Autores
Almeida, Juliana F.; Mariat, Denis; Azevedo, Vasco; Miyoshi, Anderson; de Moreno, Maria Alejandra; del Carmen, Silvina Andrea; Martin, Rebeca; Langella, Philippe; Leblanc, Jean Guy Joseph; Chatel, Jean Marc
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Fibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the invasivity of recombinant strains of Lactococcus lactis that express FnBPA under the control of its constitutive promoter or driven by the strong nisin inducible expression system (NICE) were studied.
Results: It was demonstrated that the nisA promoter allows an increase of FnBPA expression on the surface of Lactococcus lactis surface, as shown by flow cytometry, which subsequently enhanced internalization and plasmid transfer properties in vitro in Caco2 cells and Bone Marrow Dendritic Cells. In vivo, the use of nisA promoter increase the plasmid transfer in cells of both the small and large intestine of mice.
Conclusion: FnBPA expression at the surface of recombinant L. lactis is positively correlated to internalization and DNA transfer properties. The recombinant strains of L. lactis that expresses FnBPA under the control of the nisin inducible expression system could thus be considered as an improved tool in the field of DNA transfer.
Fil: Almeida, Juliana F.. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Mariat, Denis. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Azevedo, Vasco. Universidade Federal Do Minas Gerais;
Fil: Miyoshi, Anderson. Universidade Federal Do Minas Gerais;
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Martin, Rebeca. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Materia
FNBPA
LACTOCOCCUS LACTIS
NIS A PROMOTER
CACO-2 CELLS
BMDCs
INVASIVENESS
PLASMID TRANSFER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20554
Acceder
id CONICETDig_e9b58532e3bdcb59603479636df228da
oai_identifier_str oai:ri.conicet.gov.ar:11336/20554
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivoAlmeida, Juliana F.Mariat, DenisAzevedo, VascoMiyoshi, Andersonde Moreno, Maria Alejandradel Carmen, Silvina AndreaMartin, RebecaLangella, PhilippeLeblanc, Jean Guy JosephChatel, Jean MarcFNBPALACTOCOCCUS LACTISNIS A PROMOTERCACO-2 CELLSBMDCsINVASIVENESSPLASMID TRANSFERhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Background: Fibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the invasivity of recombinant strains of Lactococcus lactis that express FnBPA under the control of its constitutive promoter or driven by the strong nisin inducible expression system (NICE) were studied.<br />Results: It was demonstrated that the nisA promoter allows an increase of FnBPA expression on the surface of Lactococcus lactis surface, as shown by flow cytometry, which subsequently enhanced internalization and plasmid transfer properties in vitro in Caco2 cells and Bone Marrow Dendritic Cells. In vivo, the use of nisA promoter increase the plasmid transfer in cells of both the small and large intestine of mice.<br />Conclusion: FnBPA expression at the surface of recombinant L. lactis is positively correlated to internalization and DNA transfer properties. The recombinant strains of L. lactis that expresses FnBPA under the control of the nisin inducible expression system could thus be considered as an improved tool in the field of DNA transfer.Fil: Almeida, Juliana F.. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; FranciaFil: Mariat, Denis. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; FranciaFil: Azevedo, Vasco. Universidade Federal Do Minas Gerais;Fil: Miyoshi, Anderson. Universidade Federal Do Minas Gerais;Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Martin, Rebeca. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; FranciaFil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; FranciaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; FranciaBioMed Central2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20554Almeida, Juliana F.; Mariat, Denis; Azevedo, Vasco; Miyoshi, Anderson; de Moreno, Maria Alejandra; et al.; Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo; BioMed Central; Bmc Microbiology; 14; 248; 9-2014; 1-81471-21801471-2180CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-014-0248-9info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-014-0248-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:38Zoai:ri.conicet.gov.ar:11336/20554instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:38.902CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
title Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
spellingShingle Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
Almeida, Juliana F.
FNBPA
LACTOCOCCUS LACTIS
NIS A PROMOTER
CACO-2 CELLS
BMDCs
INVASIVENESS
PLASMID TRANSFER
title_short Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
title_full Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
title_fullStr Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
title_full_unstemmed Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
title_sort Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo
dc.creator.none.fl_str_mv Almeida, Juliana F.
Mariat, Denis
Azevedo, Vasco
Miyoshi, Anderson
de Moreno, Maria Alejandra
del Carmen, Silvina Andrea
Martin, Rebeca
Langella, Philippe
Leblanc, Jean Guy Joseph
Chatel, Jean Marc
author Almeida, Juliana F.
author_facet Almeida, Juliana F.
Mariat, Denis
Azevedo, Vasco
Miyoshi, Anderson
de Moreno, Maria Alejandra
del Carmen, Silvina Andrea
Martin, Rebeca
Langella, Philippe
Leblanc, Jean Guy Joseph
Chatel, Jean Marc
author_role author
author2 Mariat, Denis
Azevedo, Vasco
Miyoshi, Anderson
de Moreno, Maria Alejandra
del Carmen, Silvina Andrea
Martin, Rebeca
Langella, Philippe
Leblanc, Jean Guy Joseph
Chatel, Jean Marc
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FNBPA
LACTOCOCCUS LACTIS
NIS A PROMOTER
CACO-2 CELLS
BMDCs
INVASIVENESS
PLASMID TRANSFER
topic FNBPA
LACTOCOCCUS LACTIS
NIS A PROMOTER
CACO-2 CELLS
BMDCs
INVASIVENESS
PLASMID TRANSFER
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Fibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the invasivity of recombinant strains of Lactococcus lactis that express FnBPA under the control of its constitutive promoter or driven by the strong nisin inducible expression system (NICE) were studied.<br />Results: It was demonstrated that the nisA promoter allows an increase of FnBPA expression on the surface of Lactococcus lactis surface, as shown by flow cytometry, which subsequently enhanced internalization and plasmid transfer properties in vitro in Caco2 cells and Bone Marrow Dendritic Cells. In vivo, the use of nisA promoter increase the plasmid transfer in cells of both the small and large intestine of mice.<br />Conclusion: FnBPA expression at the surface of recombinant L. lactis is positively correlated to internalization and DNA transfer properties. The recombinant strains of L. lactis that expresses FnBPA under the control of the nisin inducible expression system could thus be considered as an improved tool in the field of DNA transfer.
Fil: Almeida, Juliana F.. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Mariat, Denis. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Azevedo, Vasco. Universidade Federal Do Minas Gerais;
Fil: Miyoshi, Anderson. Universidade Federal Do Minas Gerais;
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Martin, Rebeca. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Chatel, Jean Marc. Institut National de la Recherche Agronomique; Francia. Institut des sciences et industries du vivant et de l; Francia
description Background: Fibronectin Binding Protein A (FnBPA) is an invasin from Staphylococcus aureus that allows this pathogen to internalize into eukaryote cells. It was previously demonstrated that recombinant Lactococcus lactis expressing FnBPA were invasive and able to transfer a plasmid to eukaryotic cells in vitro and in vivo. In this study, the invasivity of recombinant strains of Lactococcus lactis that express FnBPA under the control of its constitutive promoter or driven by the strong nisin inducible expression system (NICE) were studied.<br />Results: It was demonstrated that the nisA promoter allows an increase of FnBPA expression on the surface of Lactococcus lactis surface, as shown by flow cytometry, which subsequently enhanced internalization and plasmid transfer properties in vitro in Caco2 cells and Bone Marrow Dendritic Cells. In vivo, the use of nisA promoter increase the plasmid transfer in cells of both the small and large intestine of mice.<br />Conclusion: FnBPA expression at the surface of recombinant L. lactis is positively correlated to internalization and DNA transfer properties. The recombinant strains of L. lactis that expresses FnBPA under the control of the nisin inducible expression system could thus be considered as an improved tool in the field of DNA transfer.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20554
Almeida, Juliana F.; Mariat, Denis; Azevedo, Vasco; Miyoshi, Anderson; de Moreno, Maria Alejandra; et al.; Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo; BioMed Central; Bmc Microbiology; 14; 248; 9-2014; 1-8
1471-2180
1471-2180
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20554
identifier_str_mv Almeida, Juliana F.; Mariat, Denis; Azevedo, Vasco; Miyoshi, Anderson; de Moreno, Maria Alejandra; et al.; Correlation between fibronectin binding protein A expression level at the surface of recombinant lactococcus lactis and plasmid transfer in vitro and in vivo; BioMed Central; Bmc Microbiology; 14; 248; 9-2014; 1-8
1471-2180
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-014-0248-9
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12866-014-0248-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782232380833792
score 12.982451

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