Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions

Autores
Pallarola, Diego Andres; Bochen, Alexander; Guglielmotti, Victoria; Oswald, Tabea A.; Kessler, Horst; Spatz, Joachim P.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The formation of new types of sensitive conductive surfaces for the detection and transduction of cell–extracellular matrix recognition events in a real time, label-free manner is of great interest in the field of biomedical research. To study molecularly defined cell functions, biologically inspired materials that mimic the nanoscale order of extracellular matrix protein fibers and yield suitable electrical charge transfer characteristics are highly desired. Our strategy to achieve this goal is based on the spatial self-organization of patches of cell-adhesive molecules onto a gold-nanoparticle-patterned indium tin oxide electrode. Fibroblast adhesion response to selective ligands for integrins α5β1 and αvβ3, which are both relevant in cancer progression, is investigated by simultaneous electrochemical impedance spectroscopy and optical microscopy. Adhesive cells on α5β1-selective nanopatterns showed enhanced membrane dynamics and tighter binding, compared with cells on αvβ3-selective nanopatterns. The surface of the electrode exhibits high sensitivity to small changes in surface properties, because of the constitution of specific cell-surface interactions. Moreover, such sensitivity enables differentiation between cell types. This is exemplified by analyzing distinct features in the electrochemical readout of MCF-7 breast cancer cells versus MCF-10A mammary epithelial cells, when subjected to individual adhesive nanopatches.
Fil: Pallarola, Diego Andres. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bochen, Alexander. Technische Universitat Munchen; Alemania
Fil: Guglielmotti, Victoria. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina
Fil: Oswald, Tabea A.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; Alemania
Fil: Kessler, Horst. Technische Universitat Munchen; Alemania
Fil: Spatz, Joachim P.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; Alemania
Materia
Electrochemical Impedance Sensor
Cell Adhesion
Integrins
Gold Nanopatterns
Transparent Microelectrodes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41153
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41153
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell InteractionsPallarola, Diego AndresBochen, AlexanderGuglielmotti, VictoriaOswald, Tabea A.Kessler, HorstSpatz, Joachim P.Electrochemical Impedance SensorCell AdhesionIntegrinsGold NanopatternsTransparent Microelectrodeshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The formation of new types of sensitive conductive surfaces for the detection and transduction of cell–extracellular matrix recognition events in a real time, label-free manner is of great interest in the field of biomedical research. To study molecularly defined cell functions, biologically inspired materials that mimic the nanoscale order of extracellular matrix protein fibers and yield suitable electrical charge transfer characteristics are highly desired. Our strategy to achieve this goal is based on the spatial self-organization of patches of cell-adhesive molecules onto a gold-nanoparticle-patterned indium tin oxide electrode. Fibroblast adhesion response to selective ligands for integrins α5β1 and αvβ3, which are both relevant in cancer progression, is investigated by simultaneous electrochemical impedance spectroscopy and optical microscopy. Adhesive cells on α5β1-selective nanopatterns showed enhanced membrane dynamics and tighter binding, compared with cells on αvβ3-selective nanopatterns. The surface of the electrode exhibits high sensitivity to small changes in surface properties, because of the constitution of specific cell-surface interactions. Moreover, such sensitivity enables differentiation between cell types. This is exemplified by analyzing distinct features in the electrochemical readout of MCF-7 breast cancer cells versus MCF-10A mammary epithelial cells, when subjected to individual adhesive nanopatches.Fil: Pallarola, Diego Andres. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bochen, Alexander. Technische Universitat Munchen; AlemaniaFil: Guglielmotti, Victoria. Universidad Nacional de San Martin. Instituto de Nanosistemas; ArgentinaFil: Oswald, Tabea A.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; AlemaniaFil: Kessler, Horst. Technische Universitat Munchen; AlemaniaFil: Spatz, Joachim P.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; AlemaniaAmerican Chemical Society2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41153Pallarola, Diego Andres; Bochen, Alexander; Guglielmotti, Victoria; Oswald, Tabea A.; Kessler, Horst; et al.; Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions; American Chemical Society; Analytical Chemistry; 89; 18; 9-2017; 10054-100620003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acs.analchem.7b02743info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.7b02743info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:31Zoai:ri.conicet.gov.ar:11336/41153instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:32.075CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
title Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
spellingShingle Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
Pallarola, Diego Andres
Electrochemical Impedance Sensor
Cell Adhesion
Integrins
Gold Nanopatterns
Transparent Microelectrodes
title_short Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
title_full Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
title_fullStr Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
title_full_unstemmed Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
title_sort Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions
dc.creator.none.fl_str_mv Pallarola, Diego Andres
Bochen, Alexander
Guglielmotti, Victoria
Oswald, Tabea A.
Kessler, Horst
Spatz, Joachim P.
author Pallarola, Diego Andres
author_facet Pallarola, Diego Andres
Bochen, Alexander
Guglielmotti, Victoria
Oswald, Tabea A.
Kessler, Horst
Spatz, Joachim P.
author_role author
author2 Bochen, Alexander
Guglielmotti, Victoria
Oswald, Tabea A.
Kessler, Horst
Spatz, Joachim P.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Electrochemical Impedance Sensor
Cell Adhesion
Integrins
Gold Nanopatterns
Transparent Microelectrodes
topic Electrochemical Impedance Sensor
Cell Adhesion
Integrins
Gold Nanopatterns
Transparent Microelectrodes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The formation of new types of sensitive conductive surfaces for the detection and transduction of cell–extracellular matrix recognition events in a real time, label-free manner is of great interest in the field of biomedical research. To study molecularly defined cell functions, biologically inspired materials that mimic the nanoscale order of extracellular matrix protein fibers and yield suitable electrical charge transfer characteristics are highly desired. Our strategy to achieve this goal is based on the spatial self-organization of patches of cell-adhesive molecules onto a gold-nanoparticle-patterned indium tin oxide electrode. Fibroblast adhesion response to selective ligands for integrins α5β1 and αvβ3, which are both relevant in cancer progression, is investigated by simultaneous electrochemical impedance spectroscopy and optical microscopy. Adhesive cells on α5β1-selective nanopatterns showed enhanced membrane dynamics and tighter binding, compared with cells on αvβ3-selective nanopatterns. The surface of the electrode exhibits high sensitivity to small changes in surface properties, because of the constitution of specific cell-surface interactions. Moreover, such sensitivity enables differentiation between cell types. This is exemplified by analyzing distinct features in the electrochemical readout of MCF-7 breast cancer cells versus MCF-10A mammary epithelial cells, when subjected to individual adhesive nanopatches.
Fil: Pallarola, Diego Andres. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bochen, Alexander. Technische Universitat Munchen; Alemania
Fil: Guglielmotti, Victoria. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina
Fil: Oswald, Tabea A.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; Alemania
Fil: Kessler, Horst. Technische Universitat Munchen; Alemania
Fil: Spatz, Joachim P.. Max Planck Institute for Medical Research; Alemania. Heidelberg University; Alemania
description The formation of new types of sensitive conductive surfaces for the detection and transduction of cell–extracellular matrix recognition events in a real time, label-free manner is of great interest in the field of biomedical research. To study molecularly defined cell functions, biologically inspired materials that mimic the nanoscale order of extracellular matrix protein fibers and yield suitable electrical charge transfer characteristics are highly desired. Our strategy to achieve this goal is based on the spatial self-organization of patches of cell-adhesive molecules onto a gold-nanoparticle-patterned indium tin oxide electrode. Fibroblast adhesion response to selective ligands for integrins α5β1 and αvβ3, which are both relevant in cancer progression, is investigated by simultaneous electrochemical impedance spectroscopy and optical microscopy. Adhesive cells on α5β1-selective nanopatterns showed enhanced membrane dynamics and tighter binding, compared with cells on αvβ3-selective nanopatterns. The surface of the electrode exhibits high sensitivity to small changes in surface properties, because of the constitution of specific cell-surface interactions. Moreover, such sensitivity enables differentiation between cell types. This is exemplified by analyzing distinct features in the electrochemical readout of MCF-7 breast cancer cells versus MCF-10A mammary epithelial cells, when subjected to individual adhesive nanopatches.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41153
Pallarola, Diego Andres; Bochen, Alexander; Guglielmotti, Victoria; Oswald, Tabea A.; Kessler, Horst; et al.; Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions; American Chemical Society; Analytical Chemistry; 89; 18; 9-2017; 10054-10062
0003-2700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41153
identifier_str_mv Pallarola, Diego Andres; Bochen, Alexander; Guglielmotti, Victoria; Oswald, Tabea A.; Kessler, Horst; et al.; Highly Ordered Gold Nanopatterned Indium Tin Oxide Electrodes for Simultaneous Optical and Electrochemical Probing Cell Interactions; American Chemical Society; Analytical Chemistry; 89; 18; 9-2017; 10054-10062
0003-2700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.analchem.7b02743
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.7b02743
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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