Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater

Autores
Grosselli, Melina del Rosario; Aparicio, Juan Daniel; Villegas, Liliana Beatriz; González, Patricia; Almeida, César Américo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Biochemical oxygen demand (BOD) is a useful parameter for assessing the biodegradability of dissolved organic matter in water. At the same time, this parameter is used to evaluate the efficiency with which certain processes remove biodegradable natural organic matter. However, the values of BOD in saline water are very low because NaCl destroy the seed. Therefore the incorporation of a suitable selection of bacteria (which is not always easy to obtain) is required. In this sense the extremophile bacteria, particularly halophilic bacteria or halophytes can contribute to the development of this technique. The objective of this work was to develop a BOD analysis protocol for the analysis of saline effluent. Within this context a halophilic microorganisms was isolated from Salinas del Bebedero, San Luis, Argentina. Salt sample was cultured in agar Dussault and Lachance. Subsequently, the isolated colonies were cultured in medium Dussault and Lachance broth for 7 days at 37ºC with constant stirring (180 rpm). Subsequently the nucleic acid extraction method was performed by Brosius et al., based on the lysis of the cells with glass beads, and 10% SDS. To amplify the variable region V3-V5, 16S rDNA primers described as F344-R915 (Stahl et al, 1991) were used: 344F: 5?- ACG GGG CGC YGCAGCAGG GA-3 ?and 915R: 5?- GTG CTC CCC CGCCAATTC CT-3 ?; Halorubrum salsolis DNA was used as control. The size of the reaction products was characterized on agarose gel 1% and evaluated on an image analyzer. The molecular weight was determined by comparison with molecular weight marker 100 bp DNA Ladder (Promega). The PCR fragments were sequenced by Macrogen (Korea), with Reaction Kit READY PRIMS a model ABI prisma373A sequencer (PE AppliedBiosystems). The sequencing results were analyzed by comparison with 16S rRNA genes databases (GenBank). The organism under study under study showed a 100% identity to gender Haloarcula sp. One milliliter of the strain (106 cell per mL) was used as seed in the BOD analysis protocol. Standard solution of glucose-glutamic acid (BOD = 20 mg L-1) with different concentrations of NaCl (5%, 10%, 20%, 30% and 40%) were used. BOD bottles were incubated at 20 °C for 5 days. No significant differences were found among differents samples, since their organic matter content was similar (CV <2.1%). In conclusion, Haloarcula sp can be used in the determination of BOD in saline effluent.
Fil: Grosselli, Melina del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: González, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
Materia
EXTREMOPHILES
BOD
SALINE WASTEWATER
HALOARCULA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201377
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201377
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewaterGrosselli, Melina del RosarioAparicio, Juan DanielVillegas, Liliana BeatrizGonzález, PatriciaAlmeida, César AméricoEXTREMOPHILESBODSALINE WASTEWATERHALOARCULAhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Biochemical oxygen demand (BOD) is a useful parameter for assessing the biodegradability of dissolved organic matter in water. At the same time, this parameter is used to evaluate the efficiency with which certain processes remove biodegradable natural organic matter. However, the values of BOD in saline water are very low because NaCl destroy the seed. Therefore the incorporation of a suitable selection of bacteria (which is not always easy to obtain) is required. In this sense the extremophile bacteria, particularly halophilic bacteria or halophytes can contribute to the development of this technique. The objective of this work was to develop a BOD analysis protocol for the analysis of saline effluent. Within this context a halophilic microorganisms was isolated from Salinas del Bebedero, San Luis, Argentina. Salt sample was cultured in agar Dussault and Lachance. Subsequently, the isolated colonies were cultured in medium Dussault and Lachance broth for 7 days at 37ºC with constant stirring (180 rpm). Subsequently the nucleic acid extraction method was performed by Brosius et al., based on the lysis of the cells with glass beads, and 10% SDS. To amplify the variable region V3-V5, 16S rDNA primers described as F344-R915 (Stahl et al, 1991) were used: 344F: 5?- ACG GGG CGC YGCAGCAGG GA-3 ?and 915R: 5?- GTG CTC CCC CGCCAATTC CT-3 ?; Halorubrum salsolis DNA was used as control. The size of the reaction products was characterized on agarose gel 1% and evaluated on an image analyzer. The molecular weight was determined by comparison with molecular weight marker 100 bp DNA Ladder (Promega). The PCR fragments were sequenced by Macrogen (Korea), with Reaction Kit READY PRIMS a model ABI prisma373A sequencer (PE AppliedBiosystems). The sequencing results were analyzed by comparison with 16S rRNA genes databases (GenBank). The organism under study under study showed a 100% identity to gender Haloarcula sp. One milliliter of the strain (106 cell per mL) was used as seed in the BOD analysis protocol. Standard solution of glucose-glutamic acid (BOD = 20 mg L-1) with different concentrations of NaCl (5%, 10%, 20%, 30% and 40%) were used. BOD bottles were incubated at 20 °C for 5 days. No significant differences were found among differents samples, since their organic matter content was similar (CV <2.1%). In conclusion, Haloarcula sp can be used in the determination of BOD in saline effluent.Fil: Grosselli, Melina del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: González, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaXI Congreso Argentino de Microbiología GeneralCórdobaArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201377Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater; XI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2015; 54-54CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2015.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:25Zoai:ri.conicet.gov.ar:11336/201377instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:25.713CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
title Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
spellingShingle Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
Grosselli, Melina del Rosario
EXTREMOPHILES
BOD
SALINE WASTEWATER
HALOARCULA
title_short Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
title_full Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
title_fullStr Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
title_full_unstemmed Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
title_sort Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater
dc.creator.none.fl_str_mv Grosselli, Melina del Rosario
Aparicio, Juan Daniel
Villegas, Liliana Beatriz
González, Patricia
Almeida, César Américo
author Grosselli, Melina del Rosario
author_facet Grosselli, Melina del Rosario
Aparicio, Juan Daniel
Villegas, Liliana Beatriz
González, Patricia
Almeida, César Américo
author_role author
author2 Aparicio, Juan Daniel
Villegas, Liliana Beatriz
González, Patricia
Almeida, César Américo
author2_role author
author
author
author
dc.subject.none.fl_str_mv EXTREMOPHILES
BOD
SALINE WASTEWATER
HALOARCULA
topic EXTREMOPHILES
BOD
SALINE WASTEWATER
HALOARCULA
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biochemical oxygen demand (BOD) is a useful parameter for assessing the biodegradability of dissolved organic matter in water. At the same time, this parameter is used to evaluate the efficiency with which certain processes remove biodegradable natural organic matter. However, the values of BOD in saline water are very low because NaCl destroy the seed. Therefore the incorporation of a suitable selection of bacteria (which is not always easy to obtain) is required. In this sense the extremophile bacteria, particularly halophilic bacteria or halophytes can contribute to the development of this technique. The objective of this work was to develop a BOD analysis protocol for the analysis of saline effluent. Within this context a halophilic microorganisms was isolated from Salinas del Bebedero, San Luis, Argentina. Salt sample was cultured in agar Dussault and Lachance. Subsequently, the isolated colonies were cultured in medium Dussault and Lachance broth for 7 days at 37ºC with constant stirring (180 rpm). Subsequently the nucleic acid extraction method was performed by Brosius et al., based on the lysis of the cells with glass beads, and 10% SDS. To amplify the variable region V3-V5, 16S rDNA primers described as F344-R915 (Stahl et al, 1991) were used: 344F: 5?- ACG GGG CGC YGCAGCAGG GA-3 ?and 915R: 5?- GTG CTC CCC CGCCAATTC CT-3 ?; Halorubrum salsolis DNA was used as control. The size of the reaction products was characterized on agarose gel 1% and evaluated on an image analyzer. The molecular weight was determined by comparison with molecular weight marker 100 bp DNA Ladder (Promega). The PCR fragments were sequenced by Macrogen (Korea), with Reaction Kit READY PRIMS a model ABI prisma373A sequencer (PE AppliedBiosystems). The sequencing results were analyzed by comparison with 16S rRNA genes databases (GenBank). The organism under study under study showed a 100% identity to gender Haloarcula sp. One milliliter of the strain (106 cell per mL) was used as seed in the BOD analysis protocol. Standard solution of glucose-glutamic acid (BOD = 20 mg L-1) with different concentrations of NaCl (5%, 10%, 20%, 30% and 40%) were used. BOD bottles were incubated at 20 °C for 5 days. No significant differences were found among differents samples, since their organic matter content was similar (CV <2.1%). In conclusion, Haloarcula sp can be used in the determination of BOD in saline effluent.
Fil: Grosselli, Melina del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aparicio, Juan Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: González, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Almeida, César Américo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XI Congreso Argentino de Microbiología General
Córdoba
Argentina
Sociedad Argentina de Microbiología General
description Biochemical oxygen demand (BOD) is a useful parameter for assessing the biodegradability of dissolved organic matter in water. At the same time, this parameter is used to evaluate the efficiency with which certain processes remove biodegradable natural organic matter. However, the values of BOD in saline water are very low because NaCl destroy the seed. Therefore the incorporation of a suitable selection of bacteria (which is not always easy to obtain) is required. In this sense the extremophile bacteria, particularly halophilic bacteria or halophytes can contribute to the development of this technique. The objective of this work was to develop a BOD analysis protocol for the analysis of saline effluent. Within this context a halophilic microorganisms was isolated from Salinas del Bebedero, San Luis, Argentina. Salt sample was cultured in agar Dussault and Lachance. Subsequently, the isolated colonies were cultured in medium Dussault and Lachance broth for 7 days at 37ºC with constant stirring (180 rpm). Subsequently the nucleic acid extraction method was performed by Brosius et al., based on the lysis of the cells with glass beads, and 10% SDS. To amplify the variable region V3-V5, 16S rDNA primers described as F344-R915 (Stahl et al, 1991) were used: 344F: 5?- ACG GGG CGC YGCAGCAGG GA-3 ?and 915R: 5?- GTG CTC CCC CGCCAATTC CT-3 ?; Halorubrum salsolis DNA was used as control. The size of the reaction products was characterized on agarose gel 1% and evaluated on an image analyzer. The molecular weight was determined by comparison with molecular weight marker 100 bp DNA Ladder (Promega). The PCR fragments were sequenced by Macrogen (Korea), with Reaction Kit READY PRIMS a model ABI prisma373A sequencer (PE AppliedBiosystems). The sequencing results were analyzed by comparison with 16S rRNA genes databases (GenBank). The organism under study under study showed a 100% identity to gender Haloarcula sp. One milliliter of the strain (106 cell per mL) was used as seed in the BOD analysis protocol. Standard solution of glucose-glutamic acid (BOD = 20 mg L-1) with different concentrations of NaCl (5%, 10%, 20%, 30% and 40%) were used. BOD bottles were incubated at 20 °C for 5 days. No significant differences were found among differents samples, since their organic matter content was similar (CV <2.1%). In conclusion, Haloarcula sp can be used in the determination of BOD in saline effluent.
publishDate 2015
dc.date.none.fl_str_mv 2015
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info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201377
Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater; XI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2015; 54-54
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201377
identifier_str_mv Isolation and use of extremophiles in the determination of biochemical oxygen demand in saline wastewater; XI Congreso Argentino de Microbiología General; Córdoba; Argentina; 2015; 54-54
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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