Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions

Autores
Gilardoni, Mónica Beatriz; Remedi, Maria Monica; Oviedo, Mabel; Dellavedova, Tristán; Sarría, Juan P.; Racca, Laura; Dominguez, Mariana; Pellizas, Claudia Gabriela; Donadio, Ana Carolina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.
Fil: Gilardoni, Mónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Oviedo, Mabel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Dellavedova, Tristán. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Sarría, Juan P.. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Racca, Laura. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Dominguez, Mariana. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Pellizas, Claudia Gabriela. Universidad Nacional de Córdoba; Argentina
Fil: Donadio, Ana Carolina. Universidad Nacional de Córdoba; Argentina
Materia
Cancer
Immunohistochemistry
Lrp-1
Mmp-9
Prostate
Zymography
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46822
Acceder
id CONICETDig_e791206916b603205ef96c1bb9017dd3
oai_identifier_str oai:ri.conicet.gov.ar:11336/46822
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesionsGilardoni, Mónica BeatrizRemedi, Maria MonicaOviedo, MabelDellavedova, TristánSarría, Juan P.Racca, LauraDominguez, MarianaPellizas, Claudia GabrielaDonadio, Ana CarolinaCancerImmunohistochemistryLrp-1Mmp-9ProstateZymographyhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.Fil: Gilardoni, Mónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Oviedo, Mabel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Dellavedova, Tristán. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Sarría, Juan P.. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Racca, Laura. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Dominguez, Mariana. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; ArgentinaFil: Pellizas, Claudia Gabriela. Universidad Nacional de Córdoba; ArgentinaFil: Donadio, Ana Carolina. Universidad Nacional de Córdoba; ArgentinaElsevier Gmbh2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46822Gilardoni, Mónica Beatriz; Remedi, Maria Monica; Oviedo, Mabel; Dellavedova, Tristán; Sarría, Juan P.; et al.; Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions; Elsevier Gmbh; Pathology - Research And Practice; 213; 1; 1-2017; 66-710344-0338CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.prp.2016.11.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0344033816306240info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:22Zoai:ri.conicet.gov.ar:11336/46822instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:22.61CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
title Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
spellingShingle Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
Gilardoni, Mónica Beatriz
Cancer
Immunohistochemistry
Lrp-1
Mmp-9
Prostate
Zymography
title_short Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
title_full Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
title_fullStr Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
title_full_unstemmed Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
title_sort Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions
dc.creator.none.fl_str_mv Gilardoni, Mónica Beatriz
Remedi, Maria Monica
Oviedo, Mabel
Dellavedova, Tristán
Sarría, Juan P.
Racca, Laura
Dominguez, Mariana
Pellizas, Claudia Gabriela
Donadio, Ana Carolina
author Gilardoni, Mónica Beatriz
author_facet Gilardoni, Mónica Beatriz
Remedi, Maria Monica
Oviedo, Mabel
Dellavedova, Tristán
Sarría, Juan P.
Racca, Laura
Dominguez, Mariana
Pellizas, Claudia Gabriela
Donadio, Ana Carolina
author_role author
author2 Remedi, Maria Monica
Oviedo, Mabel
Dellavedova, Tristán
Sarría, Juan P.
Racca, Laura
Dominguez, Mariana
Pellizas, Claudia Gabriela
Donadio, Ana Carolina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cancer
Immunohistochemistry
Lrp-1
Mmp-9
Prostate
Zymography
topic Cancer
Immunohistochemistry
Lrp-1
Mmp-9
Prostate
Zymography
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.
Fil: Gilardoni, Mónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Remedi, Maria Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Oviedo, Mabel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Dellavedova, Tristán. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Sarría, Juan P.. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Racca, Laura. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Dominguez, Mariana. Fundación Urológica Córdoba Para la Docencia E Investigación Médica; Argentina
Fil: Pellizas, Claudia Gabriela. Universidad Nacional de Córdoba; Argentina
Fil: Donadio, Ana Carolina. Universidad Nacional de Córdoba; Argentina
description Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor?related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46822
Gilardoni, Mónica Beatriz; Remedi, Maria Monica; Oviedo, Mabel; Dellavedova, Tristán; Sarría, Juan P.; et al.; Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions; Elsevier Gmbh; Pathology - Research And Practice; 213; 1; 1-2017; 66-71
0344-0338
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46822
identifier_str_mv Gilardoni, Mónica Beatriz; Remedi, Maria Monica; Oviedo, Mabel; Dellavedova, Tristán; Sarría, Juan P.; et al.; Differential expression of low density lipoprotein receptor–related protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions; Elsevier Gmbh; Pathology - Research And Practice; 213; 1; 1-2017; 66-71
0344-0338
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.prp.2016.11.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0344033816306240
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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