Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila

Autores
Sarquiz, Andrea; Rivero, Cintia Wanda; Britos, Claudia Noelia; Trelles, Jorge Abel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
5-fluorouracil-2’-deoxyriboside (FUdR), an antimetabolite known as floxuridine, is a halogenated nucleoside extensively used in the clinical treatment of colon carcinoma and hepatic metastases. This drug presents low bioavailability, thus requiring large doses and frequent administration, which leads to long-lasting and severe side effects in clinical practice. In order to overcome this problem, galactosylated nucleoside analogues were obtained using immobilized β-galactosidase in Ca-alginate with yields of 80% at only 7 h. Additionally, the obtained biocatalyst was stable for 6 months in storage conditions (4 °C) and could be reused at least 16 times without loss of its activity at 30 °C. This work describes for the first time an efficient, eco-compatible and simple bioprocess for obtaining 5´-O-β-D-galactosyl-floxuridine using an immobilized biocatalyst.
Fil: Sarquiz, Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Enzyme stabilization
Floxuridine
Galactosylation
Biocatalysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98195
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophilaSarquiz, AndreaRivero, Cintia WandaBritos, Claudia NoeliaTrelles, Jorge AbelEnzyme stabilizationFloxuridineGalactosylationBiocatalysishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/15-fluorouracil-2’-deoxyriboside (FUdR), an antimetabolite known as floxuridine, is a halogenated nucleoside extensively used in the clinical treatment of colon carcinoma and hepatic metastases. This drug presents low bioavailability, thus requiring large doses and frequent administration, which leads to long-lasting and severe side effects in clinical practice. In order to overcome this problem, galactosylated nucleoside analogues were obtained using immobilized β-galactosidase in Ca-alginate with yields of 80% at only 7 h. Additionally, the obtained biocatalyst was stable for 6 months in storage conditions (4 °C) and could be reused at least 16 times without loss of its activity at 30 °C. This work describes for the first time an efficient, eco-compatible and simple bioprocess for obtaining 5´-O-β-D-galactosyl-floxuridine using an immobilized biocatalyst.Fil: Sarquiz, Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Sa2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98195Sarquiz, Andrea; Rivero, Cintia Wanda; Britos, Claudia Noelia; Trelles, Jorge Abel; Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila; Elsevier Science Sa; Journal of Fluorine Chemistry; 214; 10-2018; 58-620022-1139CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S002211391830174Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jfluchem.2018.07.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:43Zoai:ri.conicet.gov.ar:11336/98195instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:44.099CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
title Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
spellingShingle Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
Sarquiz, Andrea
Enzyme stabilization
Floxuridine
Galactosylation
Biocatalysis
title_short Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
title_full Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
title_fullStr Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
title_full_unstemmed Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
title_sort Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila
dc.creator.none.fl_str_mv Sarquiz, Andrea
Rivero, Cintia Wanda
Britos, Claudia Noelia
Trelles, Jorge Abel
author Sarquiz, Andrea
author_facet Sarquiz, Andrea
Rivero, Cintia Wanda
Britos, Claudia Noelia
Trelles, Jorge Abel
author_role author
author2 Rivero, Cintia Wanda
Britos, Claudia Noelia
Trelles, Jorge Abel
author2_role author
author
author
dc.subject.none.fl_str_mv Enzyme stabilization
Floxuridine
Galactosylation
Biocatalysis
topic Enzyme stabilization
Floxuridine
Galactosylation
Biocatalysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv 5-fluorouracil-2’-deoxyriboside (FUdR), an antimetabolite known as floxuridine, is a halogenated nucleoside extensively used in the clinical treatment of colon carcinoma and hepatic metastases. This drug presents low bioavailability, thus requiring large doses and frequent administration, which leads to long-lasting and severe side effects in clinical practice. In order to overcome this problem, galactosylated nucleoside analogues were obtained using immobilized β-galactosidase in Ca-alginate with yields of 80% at only 7 h. Additionally, the obtained biocatalyst was stable for 6 months in storage conditions (4 °C) and could be reused at least 16 times without loss of its activity at 30 °C. This work describes for the first time an efficient, eco-compatible and simple bioprocess for obtaining 5´-O-β-D-galactosyl-floxuridine using an immobilized biocatalyst.
Fil: Sarquiz, Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Cintia Wanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trelles, Jorge Abel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description 5-fluorouracil-2’-deoxyriboside (FUdR), an antimetabolite known as floxuridine, is a halogenated nucleoside extensively used in the clinical treatment of colon carcinoma and hepatic metastases. This drug presents low bioavailability, thus requiring large doses and frequent administration, which leads to long-lasting and severe side effects in clinical practice. In order to overcome this problem, galactosylated nucleoside analogues were obtained using immobilized β-galactosidase in Ca-alginate with yields of 80% at only 7 h. Additionally, the obtained biocatalyst was stable for 6 months in storage conditions (4 °C) and could be reused at least 16 times without loss of its activity at 30 °C. This work describes for the first time an efficient, eco-compatible and simple bioprocess for obtaining 5´-O-β-D-galactosyl-floxuridine using an immobilized biocatalyst.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98195
Sarquiz, Andrea; Rivero, Cintia Wanda; Britos, Claudia Noelia; Trelles, Jorge Abel; Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila; Elsevier Science Sa; Journal of Fluorine Chemistry; 214; 10-2018; 58-62
0022-1139
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98195
identifier_str_mv Sarquiz, Andrea; Rivero, Cintia Wanda; Britos, Claudia Noelia; Trelles, Jorge Abel; Biotransformation of 5´-O-β-D-galactosyl-floxuridine by immobilized β-galactosidase from Kocuria rhizophila; Elsevier Science Sa; Journal of Fluorine Chemistry; 214; 10-2018; 58-62
0022-1139
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S002211391830174X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jfluchem.2018.07.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613288625700864
score 13.070432

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