Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina

Autores
Albarracín, Virginia Helena; Alonso Vega, Pablo; Trujillo, Martha E.; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel actinomycete strain, ABOT, isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABOT developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (meso-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202T. The DNA–DNA relatedness of strain ABOT to A. eurytherma DSM 44348T was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABOT represents a novel species in the genus Amycolatopsis, for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABOT (=DSM 45259T =LMG 24814T).
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Alonso Vega, Pablo. Universidad de Salamanca; España
Fil: Trujillo, Martha E.. Universidad de Salamanca; España
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Amycolatopsis Tucumanensis
Copper
Sediments
Bacterium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42165
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42165
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, ArgentinaAlbarracín, Virginia HelenaAlonso Vega, PabloTrujillo, Martha E.Amoroso, Maria Julia del R.Abate, Carlos MauricioAmycolatopsis TucumanensisCopperSedimentsBacteriumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A novel actinomycete strain, ABOT, isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABOT developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (meso-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202T. The DNA–DNA relatedness of strain ABOT to A. eurytherma DSM 44348T was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABOT represents a novel species in the genus Amycolatopsis, for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABOT (=DSM 45259T =LMG 24814T).Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Alonso Vega, Pablo. Universidad de Salamanca; EspañaFil: Trujillo, Martha E.. Universidad de Salamanca; EspañaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaSociety for General Microbiology2009-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42165Albarracín, Virginia Helena; Alonso Vega, Pablo; Trujillo, Martha E.; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio; Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 60; 2; 8-2009; 397-4011466-5026CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010587-0info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.010587-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:18Zoai:ri.conicet.gov.ar:11336/42165instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:18.544CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
title Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
spellingShingle Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
Albarracín, Virginia Helena
Amycolatopsis Tucumanensis
Copper
Sediments
Bacterium
title_short Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
title_full Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
title_fullStr Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
title_full_unstemmed Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
title_sort Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina
dc.creator.none.fl_str_mv Albarracín, Virginia Helena
Alonso Vega, Pablo
Trujillo, Martha E.
Amoroso, Maria Julia del R.
Abate, Carlos Mauricio
author Albarracín, Virginia Helena
author_facet Albarracín, Virginia Helena
Alonso Vega, Pablo
Trujillo, Martha E.
Amoroso, Maria Julia del R.
Abate, Carlos Mauricio
author_role author
author2 Alonso Vega, Pablo
Trujillo, Martha E.
Amoroso, Maria Julia del R.
Abate, Carlos Mauricio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Amycolatopsis Tucumanensis
Copper
Sediments
Bacterium
topic Amycolatopsis Tucumanensis
Copper
Sediments
Bacterium
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A novel actinomycete strain, ABOT, isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABOT developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (meso-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202T. The DNA–DNA relatedness of strain ABOT to A. eurytherma DSM 44348T was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABOT represents a novel species in the genus Amycolatopsis, for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABOT (=DSM 45259T =LMG 24814T).
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Alonso Vega, Pablo. Universidad de Salamanca; España
Fil: Trujillo, Martha E.. Universidad de Salamanca; España
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description A novel actinomycete strain, ABOT, isolated from copper-polluted sediments showed remarkable copper resistance as well as high bioaccumulation abilities. Classical taxonomic methods, including chemotaxonomy and molecular techniques, were used to characterize the isolate. Strain ABOT developed a honey-yellow substrate mycelium on all ISP media tested. Abundant, white, aerial mycelium was only formed on ISP 2, 5 and 7 and MM agar. Both types of hyphae fragmented into squarish rod-shaped elements. The aerial mycelium displayed spore-like structures with smooth surfaces in long, straight to flexuous chains. The organism has a type-IV cell wall lacking mycolic acids and type-A whole-cell sugar pattern (meso-diaminopimelic acid, arabinose and galactose) in addition to a phospholipid type-II profile. 16S rRNA gene sequence studies indicated that this organism is a member of the family Pseudonocardiaceae and that it forms a monophyletic clade with Amycolatopsis eurytherma NT202T. The DNA–DNA relatedness of strain ABOT to A. eurytherma DSM 44348T was 39.5 %. It is evident from these genotypic and phenotypic data that strain ABOT represents a novel species in the genus Amycolatopsis, for which the name proposed is Amycolatopsis tucumanensis sp. nov. The type strain is ABOT (=DSM 45259T =LMG 24814T).
publishDate 2009
dc.date.none.fl_str_mv 2009-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42165
Albarracín, Virginia Helena; Alonso Vega, Pablo; Trujillo, Martha E.; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio; Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 60; 2; 8-2009; 397-401
1466-5026
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42165
identifier_str_mv Albarracín, Virginia Helena; Alonso Vega, Pablo; Trujillo, Martha E.; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio; Amycolatopsis tucumanensis sp. nov., a copper resistant actinobacterium isolated from polluted sediments in Tucumán, Argentina; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 60; 2; 8-2009; 397-401
1466-5026
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.010587-0
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijs.0.010587-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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