Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression

Autores
Boccaccini, Alessandra; Legris, Martina; Krahmer, Johanna; Allenbach-Petrolati, Laure; Goyal, Anupama; Galvan Ampudia, Carlos; Vernoux, Teva; Karayekov, Elizabeth Irene; Casal, Jorge José; Fankhausera, Christian
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shade-avoiding plants, including Arabidopsis (Arabidopsis thaliana), display a number of growth responses, such as elongation of stem-like structures and repositioning of leaves, elicited by shade cues, including a reduction in the blue and red portions of the solar spectrum and a low-red to far-red ratio. Shade also promotes phototropism of de-etiolated seedlings through repression of phytochrome B, presumably to enhance capture of unfiltered sunlight. Here we show that both low blue light and a low-red to far-red light ratio are required to rapidly enhance phototropism in Arabidopsis seedlings. However, prolonged low blue light treatments are sufficient to promote phototropism through reduced cryptochrome1 (cry1) activation. The enhanced phototropic response of cry1 mutants in the lab and in response to natural canopies depends on PHYTOCHROME INTERACTING FACTORs (PIFs). In favorable light conditions, cry1 limits the expression of PIF4, while in low blue light, PIF4 expression increases, which contributes to phototropic enhancement. The analysis of quantitative DII-Venus, an auxin signaling reporter, indicates that low blue light leads to enhanced auxin signaling in the hypocotyl and, upon phototropic stimulation, a steeper auxin signaling gradient across the hypocotyl. We conclude that phototropic enhancement by canopy shade results from the combined activities of phytochrome B and cry1 that converge on PIF regulation.
Fil: Boccaccini, Alessandra. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Legris, Martina. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krahmer, Johanna. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Allenbach-Petrolati, Laure. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Goyal, Anupama. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Galvan Ampudia, Carlos. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Vernoux, Teva. Universite Lyon 2; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Karayekov, Elizabeth Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Fankhausera, Christian. Universite de Lausanne; Suiza
Materia
cryptochrome
PIF4
blue light
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168435
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/168435
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expressionBoccaccini, AlessandraLegris, MartinaKrahmer, JohannaAllenbach-Petrolati, LaureGoyal, AnupamaGalvan Ampudia, CarlosVernoux, TevaKarayekov, Elizabeth IreneCasal, Jorge JoséFankhausera, ChristiancryptochromePIF4blue lighthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Shade-avoiding plants, including Arabidopsis (Arabidopsis thaliana), display a number of growth responses, such as elongation of stem-like structures and repositioning of leaves, elicited by shade cues, including a reduction in the blue and red portions of the solar spectrum and a low-red to far-red ratio. Shade also promotes phototropism of de-etiolated seedlings through repression of phytochrome B, presumably to enhance capture of unfiltered sunlight. Here we show that both low blue light and a low-red to far-red light ratio are required to rapidly enhance phototropism in Arabidopsis seedlings. However, prolonged low blue light treatments are sufficient to promote phototropism through reduced cryptochrome1 (cry1) activation. The enhanced phototropic response of cry1 mutants in the lab and in response to natural canopies depends on PHYTOCHROME INTERACTING FACTORs (PIFs). In favorable light conditions, cry1 limits the expression of PIF4, while in low blue light, PIF4 expression increases, which contributes to phototropic enhancement. The analysis of quantitative DII-Venus, an auxin signaling reporter, indicates that low blue light leads to enhanced auxin signaling in the hypocotyl and, upon phototropic stimulation, a steeper auxin signaling gradient across the hypocotyl. We conclude that phototropic enhancement by canopy shade results from the combined activities of phytochrome B and cry1 that converge on PIF regulation.Fil: Boccaccini, Alessandra. Université de Lausanne. Faculté de Biologie et de Médecine; SuizaFil: Legris, Martina. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Krahmer, Johanna. Université de Lausanne. Faculté de Biologie et de Médecine; SuizaFil: Allenbach-Petrolati, Laure. Université de Lausanne. Faculté de Biologie et de Médecine; SuizaFil: Goyal, Anupama. Université de Lausanne. Faculté de Biologie et de Médecine; SuizaFil: Galvan Ampudia, Carlos. Université de Lausanne. Faculté de Biologie et de Médecine; SuizaFil: Vernoux, Teva. Universite Lyon 2; Francia. Centre National de la Recherche Scientifique; FranciaFil: Karayekov, Elizabeth Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Fankhausera, Christian. Universite de Lausanne; SuizaAmerican Society of Plant Biologist2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168435Boccaccini, Alessandra; Legris, Martina; Krahmer, Johanna; Allenbach-Petrolati, Laure; Goyal, Anupama; et al.; Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression; American Society of Plant Biologist; Plant Physiology; 183; 4; 6-2020; 1780-17930032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/lookup/doi/10.1104/pp.20.00243info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.20.00243info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:22Zoai:ri.conicet.gov.ar:11336/168435instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:22.754CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
title Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
spellingShingle Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
Boccaccini, Alessandra
cryptochrome
PIF4
blue light
title_short Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
title_full Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
title_fullStr Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
title_full_unstemmed Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
title_sort Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression
dc.creator.none.fl_str_mv Boccaccini, Alessandra
Legris, Martina
Krahmer, Johanna
Allenbach-Petrolati, Laure
Goyal, Anupama
Galvan Ampudia, Carlos
Vernoux, Teva
Karayekov, Elizabeth Irene
Casal, Jorge José
Fankhausera, Christian
author Boccaccini, Alessandra
author_facet Boccaccini, Alessandra
Legris, Martina
Krahmer, Johanna
Allenbach-Petrolati, Laure
Goyal, Anupama
Galvan Ampudia, Carlos
Vernoux, Teva
Karayekov, Elizabeth Irene
Casal, Jorge José
Fankhausera, Christian
author_role author
author2 Legris, Martina
Krahmer, Johanna
Allenbach-Petrolati, Laure
Goyal, Anupama
Galvan Ampudia, Carlos
Vernoux, Teva
Karayekov, Elizabeth Irene
Casal, Jorge José
Fankhausera, Christian
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv cryptochrome
PIF4
blue light
topic cryptochrome
PIF4
blue light
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Shade-avoiding plants, including Arabidopsis (Arabidopsis thaliana), display a number of growth responses, such as elongation of stem-like structures and repositioning of leaves, elicited by shade cues, including a reduction in the blue and red portions of the solar spectrum and a low-red to far-red ratio. Shade also promotes phototropism of de-etiolated seedlings through repression of phytochrome B, presumably to enhance capture of unfiltered sunlight. Here we show that both low blue light and a low-red to far-red light ratio are required to rapidly enhance phototropism in Arabidopsis seedlings. However, prolonged low blue light treatments are sufficient to promote phototropism through reduced cryptochrome1 (cry1) activation. The enhanced phototropic response of cry1 mutants in the lab and in response to natural canopies depends on PHYTOCHROME INTERACTING FACTORs (PIFs). In favorable light conditions, cry1 limits the expression of PIF4, while in low blue light, PIF4 expression increases, which contributes to phototropic enhancement. The analysis of quantitative DII-Venus, an auxin signaling reporter, indicates that low blue light leads to enhanced auxin signaling in the hypocotyl and, upon phototropic stimulation, a steeper auxin signaling gradient across the hypocotyl. We conclude that phototropic enhancement by canopy shade results from the combined activities of phytochrome B and cry1 that converge on PIF regulation.
Fil: Boccaccini, Alessandra. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Legris, Martina. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krahmer, Johanna. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Allenbach-Petrolati, Laure. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Goyal, Anupama. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Galvan Ampudia, Carlos. Université de Lausanne. Faculté de Biologie et de Médecine; Suiza
Fil: Vernoux, Teva. Universite Lyon 2; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Karayekov, Elizabeth Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Fankhausera, Christian. Universite de Lausanne; Suiza
description Shade-avoiding plants, including Arabidopsis (Arabidopsis thaliana), display a number of growth responses, such as elongation of stem-like structures and repositioning of leaves, elicited by shade cues, including a reduction in the blue and red portions of the solar spectrum and a low-red to far-red ratio. Shade also promotes phototropism of de-etiolated seedlings through repression of phytochrome B, presumably to enhance capture of unfiltered sunlight. Here we show that both low blue light and a low-red to far-red light ratio are required to rapidly enhance phototropism in Arabidopsis seedlings. However, prolonged low blue light treatments are sufficient to promote phototropism through reduced cryptochrome1 (cry1) activation. The enhanced phototropic response of cry1 mutants in the lab and in response to natural canopies depends on PHYTOCHROME INTERACTING FACTORs (PIFs). In favorable light conditions, cry1 limits the expression of PIF4, while in low blue light, PIF4 expression increases, which contributes to phototropic enhancement. The analysis of quantitative DII-Venus, an auxin signaling reporter, indicates that low blue light leads to enhanced auxin signaling in the hypocotyl and, upon phototropic stimulation, a steeper auxin signaling gradient across the hypocotyl. We conclude that phototropic enhancement by canopy shade results from the combined activities of phytochrome B and cry1 that converge on PIF regulation.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168435
Boccaccini, Alessandra; Legris, Martina; Krahmer, Johanna; Allenbach-Petrolati, Laure; Goyal, Anupama; et al.; Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression; American Society of Plant Biologist; Plant Physiology; 183; 4; 6-2020; 1780-1793
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168435
identifier_str_mv Boccaccini, Alessandra; Legris, Martina; Krahmer, Johanna; Allenbach-Petrolati, Laure; Goyal, Anupama; et al.; Low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition of PIF4 expression; American Society of Plant Biologist; Plant Physiology; 183; 4; 6-2020; 1780-1793
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantphysiol.org/lookup/doi/10.1104/pp.20.00243
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.20.00243
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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