Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir

Autores
Gerbino, Oscar Esteban; Carasi, Paula; Araujo Andrade, Cuauhtémoc; Tymczyszyn, Emma Elizabeth; Gomez Zavaglia, Andrea
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of S-layer proteins (SLP) on the Pb2+ sequestrant capacity by Lactobacillus kefir CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0–0.5 mM Pb(NO3)2. The maximum binding capacity (qmax) and the affinity coefficient (b) were calculated using the Langmuir equation. The structural effect of Pb2+ on microorganisms with and without SLP was determined using Raman spectroscopy. The bacterial interaction with Pb2+ led to a broadening in the phosphate bands (1,300–1,200 cm−1 region) and strong alterations on amide and carboxylate-related bands (νCOO− as and νCOO− s). Microorganisms without SLP removed higher percentages of Pb2+ and had higher qmax than those bearing SLP. Isolated SLP had much lower qmax and also removed lower percentages of Pb2+ than the corresponding whole microorganisms. The hydrofobicity of both strains dramatically dropped when removing SLP. When bearing SLP, strains do not expose a large amount of charged groups on their surfaces, thus making less efficient the Pb2+ removal. On the contrary, the extremely low hydrofobicity of microorganisms without SLP (and consequently, their higher capacity to remove Pb2+) can be explained on the basis of a greater exposure of charged chemical groups for the interaction with Pb2+. The viability of bacteria without SLP was not significantly lower than that of bacteria bearing SLP. However, microorganisms without SLP were more prone to the detrimental effect of Pb2+, thus suggesting that SLP acts as a protective rather than as a sequestrant layer.
Fil: Gerbino, Oscar Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina
Fil: Carasi, Paula. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Microbiología; Argentina
Fil: Araujo Andrade, Cuauhtémoc. Universidad Autonoma de Zacatecas; México
Fil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina
Fil: Gomez Zavaglia, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina
Materia
BIOSORPTION
LACTOBACILLUS KEFIR
LEAD
S-LAYER PROTEINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10104
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10104
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefirGerbino, Oscar EstebanCarasi, PaulaAraujo Andrade, CuauhtémocTymczyszyn, Emma ElizabethGomez Zavaglia, AndreaBIOSORPTIONLACTOBACILLUS KEFIRLEADS-LAYER PROTEINShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The role of S-layer proteins (SLP) on the Pb2+ sequestrant capacity by Lactobacillus kefir CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0–0.5 mM Pb(NO3)2. The maximum binding capacity (qmax) and the affinity coefficient (b) were calculated using the Langmuir equation. The structural effect of Pb2+ on microorganisms with and without SLP was determined using Raman spectroscopy. The bacterial interaction with Pb2+ led to a broadening in the phosphate bands (1,300–1,200 cm−1 region) and strong alterations on amide and carboxylate-related bands (νCOO− as and νCOO− s). Microorganisms without SLP removed higher percentages of Pb2+ and had higher qmax than those bearing SLP. Isolated SLP had much lower qmax and also removed lower percentages of Pb2+ than the corresponding whole microorganisms. The hydrofobicity of both strains dramatically dropped when removing SLP. When bearing SLP, strains do not expose a large amount of charged groups on their surfaces, thus making less efficient the Pb2+ removal. On the contrary, the extremely low hydrofobicity of microorganisms without SLP (and consequently, their higher capacity to remove Pb2+) can be explained on the basis of a greater exposure of charged chemical groups for the interaction with Pb2+. The viability of bacteria without SLP was not significantly lower than that of bacteria bearing SLP. However, microorganisms without SLP were more prone to the detrimental effect of Pb2+, thus suggesting that SLP acts as a protective rather than as a sequestrant layer.Fil: Gerbino, Oscar Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); ArgentinaFil: Carasi, Paula. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Microbiología; ArgentinaFil: Araujo Andrade, Cuauhtémoc. Universidad Autonoma de Zacatecas; MéxicoFil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; ArgentinaFil: Gomez Zavaglia, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); ArgentinaSpringer2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10104Gerbino, Oscar Esteban; Carasi, Paula; Araujo Andrade, Cuauhtémoc; Tymczyszyn, Emma Elizabeth; Gomez Zavaglia, Andrea; Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir; Springer; World Journal of Microbiology; 31; 4; 2-2015; 583-5920959-39931573-0972enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-015-1812-7info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11274-015-1812-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:14Zoai:ri.conicet.gov.ar:11336/10104instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:15.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
title Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
spellingShingle Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
Gerbino, Oscar Esteban
BIOSORPTION
LACTOBACILLUS KEFIR
LEAD
S-LAYER PROTEINS
title_short Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
title_full Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
title_fullStr Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
title_full_unstemmed Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
title_sort Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir
dc.creator.none.fl_str_mv Gerbino, Oscar Esteban
Carasi, Paula
Araujo Andrade, Cuauhtémoc
Tymczyszyn, Emma Elizabeth
Gomez Zavaglia, Andrea
author Gerbino, Oscar Esteban
author_facet Gerbino, Oscar Esteban
Carasi, Paula
Araujo Andrade, Cuauhtémoc
Tymczyszyn, Emma Elizabeth
Gomez Zavaglia, Andrea
author_role author
author2 Carasi, Paula
Araujo Andrade, Cuauhtémoc
Tymczyszyn, Emma Elizabeth
Gomez Zavaglia, Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOSORPTION
LACTOBACILLUS KEFIR
LEAD
S-LAYER PROTEINS
topic BIOSORPTION
LACTOBACILLUS KEFIR
LEAD
S-LAYER PROTEINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The role of S-layer proteins (SLP) on the Pb2+ sequestrant capacity by Lactobacillus kefir CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0–0.5 mM Pb(NO3)2. The maximum binding capacity (qmax) and the affinity coefficient (b) were calculated using the Langmuir equation. The structural effect of Pb2+ on microorganisms with and without SLP was determined using Raman spectroscopy. The bacterial interaction with Pb2+ led to a broadening in the phosphate bands (1,300–1,200 cm−1 region) and strong alterations on amide and carboxylate-related bands (νCOO− as and νCOO− s). Microorganisms without SLP removed higher percentages of Pb2+ and had higher qmax than those bearing SLP. Isolated SLP had much lower qmax and also removed lower percentages of Pb2+ than the corresponding whole microorganisms. The hydrofobicity of both strains dramatically dropped when removing SLP. When bearing SLP, strains do not expose a large amount of charged groups on their surfaces, thus making less efficient the Pb2+ removal. On the contrary, the extremely low hydrofobicity of microorganisms without SLP (and consequently, their higher capacity to remove Pb2+) can be explained on the basis of a greater exposure of charged chemical groups for the interaction with Pb2+. The viability of bacteria without SLP was not significantly lower than that of bacteria bearing SLP. However, microorganisms without SLP were more prone to the detrimental effect of Pb2+, thus suggesting that SLP acts as a protective rather than as a sequestrant layer.
Fil: Gerbino, Oscar Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina
Fil: Carasi, Paula. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Microbiología; Argentina
Fil: Araujo Andrade, Cuauhtémoc. Universidad Autonoma de Zacatecas; México
Fil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Area Ingenieria en Alimentos. Laboratorio de Microbiologia de Alimentos; Argentina
Fil: Gomez Zavaglia, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina
description The role of S-layer proteins (SLP) on the Pb2+ sequestrant capacity by Lactobacillus kefir CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0–0.5 mM Pb(NO3)2. The maximum binding capacity (qmax) and the affinity coefficient (b) were calculated using the Langmuir equation. The structural effect of Pb2+ on microorganisms with and without SLP was determined using Raman spectroscopy. The bacterial interaction with Pb2+ led to a broadening in the phosphate bands (1,300–1,200 cm−1 region) and strong alterations on amide and carboxylate-related bands (νCOO− as and νCOO− s). Microorganisms without SLP removed higher percentages of Pb2+ and had higher qmax than those bearing SLP. Isolated SLP had much lower qmax and also removed lower percentages of Pb2+ than the corresponding whole microorganisms. The hydrofobicity of both strains dramatically dropped when removing SLP. When bearing SLP, strains do not expose a large amount of charged groups on their surfaces, thus making less efficient the Pb2+ removal. On the contrary, the extremely low hydrofobicity of microorganisms without SLP (and consequently, their higher capacity to remove Pb2+) can be explained on the basis of a greater exposure of charged chemical groups for the interaction with Pb2+. The viability of bacteria without SLP was not significantly lower than that of bacteria bearing SLP. However, microorganisms without SLP were more prone to the detrimental effect of Pb2+, thus suggesting that SLP acts as a protective rather than as a sequestrant layer.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10104
Gerbino, Oscar Esteban; Carasi, Paula; Araujo Andrade, Cuauhtémoc; Tymczyszyn, Emma Elizabeth; Gomez Zavaglia, Andrea; Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir; Springer; World Journal of Microbiology; 31; 4; 2-2015; 583-592
0959-3993
1573-0972
url http://hdl.handle.net/11336/10104
identifier_str_mv Gerbino, Oscar Esteban; Carasi, Paula; Araujo Andrade, Cuauhtémoc; Tymczyszyn, Emma Elizabeth; Gomez Zavaglia, Andrea; Role of S-layer proteins in the biosorption capacity of lead by Lactobacillus kefir; Springer; World Journal of Microbiology; 31; 4; 2-2015; 583-592
0959-3993
1573-0972
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-015-1812-7
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11274-015-1812-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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