Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex
- Autores
- Chang, Eileen I.; Zárate, Miguel A.; Rabaglino, Maria Belen; Richards, Elaine M.; Arndt, Thomas J.; Keller Wood, Maureen; Wood, Charles E.
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Transient hypoxia in pregnancy stimulates a physiological reflex response that redistributes blood flow and defends oxygen delivery to the fetal brain. We designed the present experiment to test the hypotheses that transient hypoxia produces damage of the cerebral cortex and that ketamine, an antagonist of NMDA receptors and a known anti-inflammatory agent, reduces the damage. Late gestation, chronically catheterized fetal sheep were subjected to a 30-min period of ventilatory hypoxia that decreased fetal PaO2 from 17 ± 1 to 10 ± 1 mmHg, or normoxia (PaO2 17 ± 1 mmHg), with or without pretreatment (10 min before hypoxia/normoxia) with ketamine (3 mg/kg, i.v.). One day (24 h) after hypoxia/normoxia, fetal cerebral cortex was removed and mRNA extracted for transcriptomics and systems biology analysis (n = 3-5 per group). Hypoxia stimulated a transcriptomic response consistent with a reduction in cellular metabolism and an increase in inflammation. Ketamine pretreatment reduced both of these responses. The inflammation response modeled with transcriptomic systems biology was validated by immunohistochemistry and showed increased abundance of microglia/macrophages after hypoxia in the cerebral cortical tissue that ketamine significantly reduced. We conclude that transient hypoxia produces inflammation of the fetal cerebral cortex and that ketamine, in a standard clinical dose, reduces the inflammation response.
Fil: Chang, Eileen I.. University of Florida; Estados Unidos
Fil: Zárate, Miguel A.. University of Florida; Estados Unidos
Fil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Richards, Elaine M.. University of Florida; Estados Unidos
Fil: Arndt, Thomas J.. University of Florida; Estados Unidos
Fil: Keller Wood, Maureen. University of Florida; Estados Unidos
Fil: Wood, Charles E.. University of Florida; Estados Unidos - Materia
-
FETAL HYPOXIA
FRONTAL CORTEX
IMMUNE RESPONSE
KETAMINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/180065
Ver los metadatos del registro completo
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Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortexChang, Eileen I.Zárate, Miguel A.Rabaglino, Maria BelenRichards, Elaine M.Arndt, Thomas J.Keller Wood, MaureenWood, Charles E.FETAL HYPOXIAFRONTAL CORTEXIMMUNE RESPONSEKETAMINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Transient hypoxia in pregnancy stimulates a physiological reflex response that redistributes blood flow and defends oxygen delivery to the fetal brain. We designed the present experiment to test the hypotheses that transient hypoxia produces damage of the cerebral cortex and that ketamine, an antagonist of NMDA receptors and a known anti-inflammatory agent, reduces the damage. Late gestation, chronically catheterized fetal sheep were subjected to a 30-min period of ventilatory hypoxia that decreased fetal PaO2 from 17 ± 1 to 10 ± 1 mmHg, or normoxia (PaO2 17 ± 1 mmHg), with or without pretreatment (10 min before hypoxia/normoxia) with ketamine (3 mg/kg, i.v.). One day (24 h) after hypoxia/normoxia, fetal cerebral cortex was removed and mRNA extracted for transcriptomics and systems biology analysis (n = 3-5 per group). Hypoxia stimulated a transcriptomic response consistent with a reduction in cellular metabolism and an increase in inflammation. Ketamine pretreatment reduced both of these responses. The inflammation response modeled with transcriptomic systems biology was validated by immunohistochemistry and showed increased abundance of microglia/macrophages after hypoxia in the cerebral cortical tissue that ketamine significantly reduced. We conclude that transient hypoxia produces inflammation of the fetal cerebral cortex and that ketamine, in a standard clinical dose, reduces the inflammation response.Fil: Chang, Eileen I.. University of Florida; Estados UnidosFil: Zárate, Miguel A.. University of Florida; Estados UnidosFil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Richards, Elaine M.. University of Florida; Estados UnidosFil: Arndt, Thomas J.. University of Florida; Estados UnidosFil: Keller Wood, Maureen. University of Florida; Estados UnidosFil: Wood, Charles E.. University of Florida; Estados UnidosWiley Blackwell Publishing, Inc2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180065Chang, Eileen I.; Zárate, Miguel A.; Rabaglino, Maria Belen; Richards, Elaine M.; Arndt, Thomas J.; et al.; Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex; Wiley Blackwell Publishing, Inc; Physiological Reports; 4; 6; 3-2016; 1-152051-817XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/full/10.14814/phy2.12741info:eu-repo/semantics/altIdentifier/doi/10.14814/phy2.12741info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:25Zoai:ri.conicet.gov.ar:11336/180065instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:25.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
title |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
spellingShingle |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex Chang, Eileen I. FETAL HYPOXIA FRONTAL CORTEX IMMUNE RESPONSE KETAMINE |
title_short |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
title_full |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
title_fullStr |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
title_full_unstemmed |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
title_sort |
Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex |
dc.creator.none.fl_str_mv |
Chang, Eileen I. Zárate, Miguel A. Rabaglino, Maria Belen Richards, Elaine M. Arndt, Thomas J. Keller Wood, Maureen Wood, Charles E. |
author |
Chang, Eileen I. |
author_facet |
Chang, Eileen I. Zárate, Miguel A. Rabaglino, Maria Belen Richards, Elaine M. Arndt, Thomas J. Keller Wood, Maureen Wood, Charles E. |
author_role |
author |
author2 |
Zárate, Miguel A. Rabaglino, Maria Belen Richards, Elaine M. Arndt, Thomas J. Keller Wood, Maureen Wood, Charles E. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
FETAL HYPOXIA FRONTAL CORTEX IMMUNE RESPONSE KETAMINE |
topic |
FETAL HYPOXIA FRONTAL CORTEX IMMUNE RESPONSE KETAMINE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Transient hypoxia in pregnancy stimulates a physiological reflex response that redistributes blood flow and defends oxygen delivery to the fetal brain. We designed the present experiment to test the hypotheses that transient hypoxia produces damage of the cerebral cortex and that ketamine, an antagonist of NMDA receptors and a known anti-inflammatory agent, reduces the damage. Late gestation, chronically catheterized fetal sheep were subjected to a 30-min period of ventilatory hypoxia that decreased fetal PaO2 from 17 ± 1 to 10 ± 1 mmHg, or normoxia (PaO2 17 ± 1 mmHg), with or without pretreatment (10 min before hypoxia/normoxia) with ketamine (3 mg/kg, i.v.). One day (24 h) after hypoxia/normoxia, fetal cerebral cortex was removed and mRNA extracted for transcriptomics and systems biology analysis (n = 3-5 per group). Hypoxia stimulated a transcriptomic response consistent with a reduction in cellular metabolism and an increase in inflammation. Ketamine pretreatment reduced both of these responses. The inflammation response modeled with transcriptomic systems biology was validated by immunohistochemistry and showed increased abundance of microglia/macrophages after hypoxia in the cerebral cortical tissue that ketamine significantly reduced. We conclude that transient hypoxia produces inflammation of the fetal cerebral cortex and that ketamine, in a standard clinical dose, reduces the inflammation response. Fil: Chang, Eileen I.. University of Florida; Estados Unidos Fil: Zárate, Miguel A.. University of Florida; Estados Unidos Fil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina Fil: Richards, Elaine M.. University of Florida; Estados Unidos Fil: Arndt, Thomas J.. University of Florida; Estados Unidos Fil: Keller Wood, Maureen. University of Florida; Estados Unidos Fil: Wood, Charles E.. University of Florida; Estados Unidos |
description |
Transient hypoxia in pregnancy stimulates a physiological reflex response that redistributes blood flow and defends oxygen delivery to the fetal brain. We designed the present experiment to test the hypotheses that transient hypoxia produces damage of the cerebral cortex and that ketamine, an antagonist of NMDA receptors and a known anti-inflammatory agent, reduces the damage. Late gestation, chronically catheterized fetal sheep were subjected to a 30-min period of ventilatory hypoxia that decreased fetal PaO2 from 17 ± 1 to 10 ± 1 mmHg, or normoxia (PaO2 17 ± 1 mmHg), with or without pretreatment (10 min before hypoxia/normoxia) with ketamine (3 mg/kg, i.v.). One day (24 h) after hypoxia/normoxia, fetal cerebral cortex was removed and mRNA extracted for transcriptomics and systems biology analysis (n = 3-5 per group). Hypoxia stimulated a transcriptomic response consistent with a reduction in cellular metabolism and an increase in inflammation. Ketamine pretreatment reduced both of these responses. The inflammation response modeled with transcriptomic systems biology was validated by immunohistochemistry and showed increased abundance of microglia/macrophages after hypoxia in the cerebral cortical tissue that ketamine significantly reduced. We conclude that transient hypoxia produces inflammation of the fetal cerebral cortex and that ketamine, in a standard clinical dose, reduces the inflammation response. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/180065 Chang, Eileen I.; Zárate, Miguel A.; Rabaglino, Maria Belen; Richards, Elaine M.; Arndt, Thomas J.; et al.; Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex; Wiley Blackwell Publishing, Inc; Physiological Reports; 4; 6; 3-2016; 1-15 2051-817X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/180065 |
identifier_str_mv |
Chang, Eileen I.; Zárate, Miguel A.; Rabaglino, Maria Belen; Richards, Elaine M.; Arndt, Thomas J.; et al.; Ketamine decreases inflammatory and immune pathways after transient hypoxia in late gestation fetal cerebral cortex; Wiley Blackwell Publishing, Inc; Physiological Reports; 4; 6; 3-2016; 1-15 2051-817X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://physoc.onlinelibrary.wiley.com/doi/full/10.14814/phy2.12741 info:eu-repo/semantics/altIdentifier/doi/10.14814/phy2.12741 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268601332006912 |
score |
13.13397 |