A gender comparison of postobstructive injury in the rat kidney

Autores
Quinlan, Mark R.; Cronin, Peter; Daly, Padraig J.; Watson, Ronald W. G.; Manucha, Walter Ariel Fernando; Docherty, Neil G.; Fitzpatrick, John M.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background/Aims: Unilateral ureteral obstruction (UUO) results in renal injury. Studies report increased injury indices in male rats following UUO. Our study examined whether this gender-based renal response to UUO was reflected in sustained differences following relief of obstruction. Methods: Adult male/female rats (200-400 g) were subjected to either sham surgery (S/RN) or UUO (UUO/RN). At 24 h, obstruc- tion was relieved and all animals underwent contralateral nephrectomy. Five days after initial surgery, animals were placed in metabolic cages and given water ad libitum for 24 h followed by a 24-hour period of complete water restriction. On day 7, animals were euthanized and samples harvested. Tubular injury, urinary volume/osmolality, creatinine clearance, plasma arginine vasopressin, renal medullary V2 receptor and aquaporin 2 (AQP2) expression were measured. Results: Male UUO/RN rats showed increased renal apoptotic injury and reduced creatinine clearance rates (glomerular filtration rate) vs. females. No gender-dependent differences were observed in urinary osmolality or concentrating ability. AQP2 expression increased post-obstruction. Conclusion: Increased injury in males following UUO remains manifest during early recovery after release of obstruction. Despite this, the grade of postobstructive diuresis is not significantly altered between sexes. This may reflect reduced glomerular filtration rate and elevated AQP2 in male rats.
Fil: Quinlan, Mark R.. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. University College Dublin; Irlanda
Fil: Cronin, Peter. University College Dublin; Irlanda
Fil: Daly, Padraig J.. University College Dublin; Irlanda. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda
Fil: Watson, Ronald W. G.. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. University College Dublin; Irlanda
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Docherty, Neil G.. Trinity College Dublin; Irlanda
Fil: Fitzpatrick, John M.. University College Dublin; Irlanda. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. Mater Misericordiae University Hospital; Irlanda
Materia
Apoptosis
Aquaporin
Diuresis
Ureteral Obstruction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79284
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79284
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A gender comparison of postobstructive injury in the rat kidneyQuinlan, Mark R.Cronin, PeterDaly, Padraig J.Watson, Ronald W. G.Manucha, Walter Ariel FernandoDocherty, Neil G.Fitzpatrick, John M.ApoptosisAquaporinDiuresisUreteral Obstructionhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background/Aims: Unilateral ureteral obstruction (UUO) results in renal injury. Studies report increased injury indices in male rats following UUO. Our study examined whether this gender-based renal response to UUO was reflected in sustained differences following relief of obstruction. Methods: Adult male/female rats (200-400 g) were subjected to either sham surgery (S/RN) or UUO (UUO/RN). At 24 h, obstruc- tion was relieved and all animals underwent contralateral nephrectomy. Five days after initial surgery, animals were placed in metabolic cages and given water ad libitum for 24 h followed by a 24-hour period of complete water restriction. On day 7, animals were euthanized and samples harvested. Tubular injury, urinary volume/osmolality, creatinine clearance, plasma arginine vasopressin, renal medullary V2 receptor and aquaporin 2 (AQP2) expression were measured. Results: Male UUO/RN rats showed increased renal apoptotic injury and reduced creatinine clearance rates (glomerular filtration rate) vs. females. No gender-dependent differences were observed in urinary osmolality or concentrating ability. AQP2 expression increased post-obstruction. Conclusion: Increased injury in males following UUO remains manifest during early recovery after release of obstruction. Despite this, the grade of postobstructive diuresis is not significantly altered between sexes. This may reflect reduced glomerular filtration rate and elevated AQP2 in male rats.Fil: Quinlan, Mark R.. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. University College Dublin; IrlandaFil: Cronin, Peter. University College Dublin; IrlandaFil: Daly, Padraig J.. University College Dublin; Irlanda. The Conway Institute Of Biomolecular And Biomedical Science; IrlandaFil: Watson, Ronald W. G.. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. University College Dublin; IrlandaFil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Docherty, Neil G.. Trinity College Dublin; IrlandaFil: Fitzpatrick, John M.. University College Dublin; Irlanda. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. Mater Misericordiae University Hospital; IrlandaKarger2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79284Quinlan, Mark R.; Cronin, Peter; Daly, Padraig J.; Watson, Ronald W. G.; Manucha, Walter Ariel Fernando; et al.; A gender comparison of postobstructive injury in the rat kidney; Karger; Kidney And Blood Pressure Research; 33; 4; 8-2010; 266-2731420-40961423-0143CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000317934info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/article/Abstract/317934info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:38Zoai:ri.conicet.gov.ar:11336/79284instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:38.783CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A gender comparison of postobstructive injury in the rat kidney
title A gender comparison of postobstructive injury in the rat kidney
spellingShingle A gender comparison of postobstructive injury in the rat kidney
Quinlan, Mark R.
Apoptosis
Aquaporin
Diuresis
Ureteral Obstruction
title_short A gender comparison of postobstructive injury in the rat kidney
title_full A gender comparison of postobstructive injury in the rat kidney
title_fullStr A gender comparison of postobstructive injury in the rat kidney
title_full_unstemmed A gender comparison of postobstructive injury in the rat kidney
title_sort A gender comparison of postobstructive injury in the rat kidney
dc.creator.none.fl_str_mv Quinlan, Mark R.
Cronin, Peter
Daly, Padraig J.
Watson, Ronald W. G.
Manucha, Walter Ariel Fernando
Docherty, Neil G.
Fitzpatrick, John M.
author Quinlan, Mark R.
author_facet Quinlan, Mark R.
Cronin, Peter
Daly, Padraig J.
Watson, Ronald W. G.
Manucha, Walter Ariel Fernando
Docherty, Neil G.
Fitzpatrick, John M.
author_role author
author2 Cronin, Peter
Daly, Padraig J.
Watson, Ronald W. G.
Manucha, Walter Ariel Fernando
Docherty, Neil G.
Fitzpatrick, John M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Aquaporin
Diuresis
Ureteral Obstruction
topic Apoptosis
Aquaporin
Diuresis
Ureteral Obstruction
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background/Aims: Unilateral ureteral obstruction (UUO) results in renal injury. Studies report increased injury indices in male rats following UUO. Our study examined whether this gender-based renal response to UUO was reflected in sustained differences following relief of obstruction. Methods: Adult male/female rats (200-400 g) were subjected to either sham surgery (S/RN) or UUO (UUO/RN). At 24 h, obstruc- tion was relieved and all animals underwent contralateral nephrectomy. Five days after initial surgery, animals were placed in metabolic cages and given water ad libitum for 24 h followed by a 24-hour period of complete water restriction. On day 7, animals were euthanized and samples harvested. Tubular injury, urinary volume/osmolality, creatinine clearance, plasma arginine vasopressin, renal medullary V2 receptor and aquaporin 2 (AQP2) expression were measured. Results: Male UUO/RN rats showed increased renal apoptotic injury and reduced creatinine clearance rates (glomerular filtration rate) vs. females. No gender-dependent differences were observed in urinary osmolality or concentrating ability. AQP2 expression increased post-obstruction. Conclusion: Increased injury in males following UUO remains manifest during early recovery after release of obstruction. Despite this, the grade of postobstructive diuresis is not significantly altered between sexes. This may reflect reduced glomerular filtration rate and elevated AQP2 in male rats.
Fil: Quinlan, Mark R.. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. University College Dublin; Irlanda
Fil: Cronin, Peter. University College Dublin; Irlanda
Fil: Daly, Padraig J.. University College Dublin; Irlanda. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda
Fil: Watson, Ronald W. G.. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. University College Dublin; Irlanda
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Docherty, Neil G.. Trinity College Dublin; Irlanda
Fil: Fitzpatrick, John M.. University College Dublin; Irlanda. The Conway Institute Of Biomolecular And Biomedical Science; Irlanda. Mater Misericordiae University Hospital; Irlanda
description Background/Aims: Unilateral ureteral obstruction (UUO) results in renal injury. Studies report increased injury indices in male rats following UUO. Our study examined whether this gender-based renal response to UUO was reflected in sustained differences following relief of obstruction. Methods: Adult male/female rats (200-400 g) were subjected to either sham surgery (S/RN) or UUO (UUO/RN). At 24 h, obstruc- tion was relieved and all animals underwent contralateral nephrectomy. Five days after initial surgery, animals were placed in metabolic cages and given water ad libitum for 24 h followed by a 24-hour period of complete water restriction. On day 7, animals were euthanized and samples harvested. Tubular injury, urinary volume/osmolality, creatinine clearance, plasma arginine vasopressin, renal medullary V2 receptor and aquaporin 2 (AQP2) expression were measured. Results: Male UUO/RN rats showed increased renal apoptotic injury and reduced creatinine clearance rates (glomerular filtration rate) vs. females. No gender-dependent differences were observed in urinary osmolality or concentrating ability. AQP2 expression increased post-obstruction. Conclusion: Increased injury in males following UUO remains manifest during early recovery after release of obstruction. Despite this, the grade of postobstructive diuresis is not significantly altered between sexes. This may reflect reduced glomerular filtration rate and elevated AQP2 in male rats.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79284
Quinlan, Mark R.; Cronin, Peter; Daly, Padraig J.; Watson, Ronald W. G.; Manucha, Walter Ariel Fernando; et al.; A gender comparison of postobstructive injury in the rat kidney; Karger; Kidney And Blood Pressure Research; 33; 4; 8-2010; 266-273
1420-4096
1423-0143
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79284
identifier_str_mv Quinlan, Mark R.; Cronin, Peter; Daly, Padraig J.; Watson, Ronald W. G.; Manucha, Walter Ariel Fernando; et al.; A gender comparison of postobstructive injury in the rat kidney; Karger; Kidney And Blood Pressure Research; 33; 4; 8-2010; 266-273
1420-4096
1423-0143
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000317934
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/article/Abstract/317934
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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