Bio-vaterite formation by glycoproteins from freshwater pearls

Autores
Natoli, Antonino; Wiens, Matthias; Schröder, Heinz Christoph; Stifanic, Mauro; Batel, Renato; Soldati, Analía Leticia; Jacob, Dorrit E.; Müller, Werner E.G.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A 48 kDa acidic and putative calcium-binding glycoprotein was isolated from pearls of the freshwater mussel Hyriopsis cumingii. This protein was compared with a related 46 kDa polypeptide, obtained from the nacreous shell of the same species. Separation by two-dimensional gel electrophoresis revealed that the difference in molecular weight is due to the higher extent of glycosylation of the 48 kDa protein existing in pearls. Evidence is presented that the sugar moieties of the protein contribute to crystal growth, starting with the nucleation step. In in vitro precipitation experiments, the 48 kDa glycoprotein of pearls directed the formation of round-shaped vaterite crystals while the 46 kDa glycoprotein of shells promoted formation of small irregular calcite particles. Furthermore, both proteins, 48 kDa/46 kDa, comprised carbonic anhydrase activity that has been implicated in CaCO3 formation. Thus, a function of the isolated glycoproteins in biomineralization is proposed together with the mechanism by which they can stabilize different calcium carbonate polymorphs.
Fil: Natoli, Antonino. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Wiens, Matthias. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Schröder, Heinz Christoph. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Stifanic, Mauro. “Ruder Boskovic” Institute; Croacia
Fil: Batel, Renato. “Ruder Boskovic” Institute; Croacia
Fil: Soldati, Analía Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Jacob, Dorrit E.. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Müller, Werner E.G.. Johannes Gutenberg Universitat Mainz; Alemania
Materia
BIOMINERALIZATION
CALCIUM CARBONATE PRECIPITATION
CARBONIC ANHYDRASE
FRESHWATER PEARLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281410
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bio-vaterite formation by glycoproteins from freshwater pearlsNatoli, AntoninoWiens, MatthiasSchröder, Heinz ChristophStifanic, MauroBatel, RenatoSoldati, Analía LeticiaJacob, Dorrit E.Müller, Werner E.G.BIOMINERALIZATIONCALCIUM CARBONATE PRECIPITATIONCARBONIC ANHYDRASEFRESHWATER PEARLShttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2A 48 kDa acidic and putative calcium-binding glycoprotein was isolated from pearls of the freshwater mussel Hyriopsis cumingii. This protein was compared with a related 46 kDa polypeptide, obtained from the nacreous shell of the same species. Separation by two-dimensional gel electrophoresis revealed that the difference in molecular weight is due to the higher extent of glycosylation of the 48 kDa protein existing in pearls. Evidence is presented that the sugar moieties of the protein contribute to crystal growth, starting with the nucleation step. In in vitro precipitation experiments, the 48 kDa glycoprotein of pearls directed the formation of round-shaped vaterite crystals while the 46 kDa glycoprotein of shells promoted formation of small irregular calcite particles. Furthermore, both proteins, 48 kDa/46 kDa, comprised carbonic anhydrase activity that has been implicated in CaCO3 formation. Thus, a function of the isolated glycoproteins in biomineralization is proposed together with the mechanism by which they can stabilize different calcium carbonate polymorphs.Fil: Natoli, Antonino. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Wiens, Matthias. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Schröder, Heinz Christoph. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Stifanic, Mauro. “Ruder Boskovic” Institute; CroaciaFil: Batel, Renato. “Ruder Boskovic” Institute; CroaciaFil: Soldati, Analía Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Jacob, Dorrit E.. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Müller, Werner E.G.. Johannes Gutenberg Universitat Mainz; AlemaniaPergamon-Elsevier Science Ltd2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281410Natoli, Antonino; Wiens, Matthias; Schröder, Heinz Christoph; Stifanic, Mauro; Batel, Renato; et al.; Bio-vaterite formation by glycoproteins from freshwater pearls; Pergamon-Elsevier Science Ltd; Micron; 41; 4; 6-2010; 359-3660968-4328CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0968432810000041info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micron.2010.01.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-04T10:51:43Zoai:ri.conicet.gov.ar:11336/281410instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-04 10:51:43.55CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bio-vaterite formation by glycoproteins from freshwater pearls
title Bio-vaterite formation by glycoproteins from freshwater pearls
spellingShingle Bio-vaterite formation by glycoproteins from freshwater pearls
Natoli, Antonino
BIOMINERALIZATION
CALCIUM CARBONATE PRECIPITATION
CARBONIC ANHYDRASE
FRESHWATER PEARLS
title_short Bio-vaterite formation by glycoproteins from freshwater pearls
title_full Bio-vaterite formation by glycoproteins from freshwater pearls
title_fullStr Bio-vaterite formation by glycoproteins from freshwater pearls
title_full_unstemmed Bio-vaterite formation by glycoproteins from freshwater pearls
title_sort Bio-vaterite formation by glycoproteins from freshwater pearls
dc.creator.none.fl_str_mv Natoli, Antonino
Wiens, Matthias
Schröder, Heinz Christoph
Stifanic, Mauro
Batel, Renato
Soldati, Analía Leticia
Jacob, Dorrit E.
Müller, Werner E.G.
author Natoli, Antonino
author_facet Natoli, Antonino
Wiens, Matthias
Schröder, Heinz Christoph
Stifanic, Mauro
Batel, Renato
Soldati, Analía Leticia
Jacob, Dorrit E.
Müller, Werner E.G.
author_role author
author2 Wiens, Matthias
Schröder, Heinz Christoph
Stifanic, Mauro
Batel, Renato
Soldati, Analía Leticia
Jacob, Dorrit E.
Müller, Werner E.G.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOMINERALIZATION
CALCIUM CARBONATE PRECIPITATION
CARBONIC ANHYDRASE
FRESHWATER PEARLS
topic BIOMINERALIZATION
CALCIUM CARBONATE PRECIPITATION
CARBONIC ANHYDRASE
FRESHWATER PEARLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A 48 kDa acidic and putative calcium-binding glycoprotein was isolated from pearls of the freshwater mussel Hyriopsis cumingii. This protein was compared with a related 46 kDa polypeptide, obtained from the nacreous shell of the same species. Separation by two-dimensional gel electrophoresis revealed that the difference in molecular weight is due to the higher extent of glycosylation of the 48 kDa protein existing in pearls. Evidence is presented that the sugar moieties of the protein contribute to crystal growth, starting with the nucleation step. In in vitro precipitation experiments, the 48 kDa glycoprotein of pearls directed the formation of round-shaped vaterite crystals while the 46 kDa glycoprotein of shells promoted formation of small irregular calcite particles. Furthermore, both proteins, 48 kDa/46 kDa, comprised carbonic anhydrase activity that has been implicated in CaCO3 formation. Thus, a function of the isolated glycoproteins in biomineralization is proposed together with the mechanism by which they can stabilize different calcium carbonate polymorphs.
Fil: Natoli, Antonino. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Wiens, Matthias. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Schröder, Heinz Christoph. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Stifanic, Mauro. “Ruder Boskovic” Institute; Croacia
Fil: Batel, Renato. “Ruder Boskovic” Institute; Croacia
Fil: Soldati, Analía Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Jacob, Dorrit E.. Johannes Gutenberg Universitat Mainz; Alemania
Fil: Müller, Werner E.G.. Johannes Gutenberg Universitat Mainz; Alemania
description A 48 kDa acidic and putative calcium-binding glycoprotein was isolated from pearls of the freshwater mussel Hyriopsis cumingii. This protein was compared with a related 46 kDa polypeptide, obtained from the nacreous shell of the same species. Separation by two-dimensional gel electrophoresis revealed that the difference in molecular weight is due to the higher extent of glycosylation of the 48 kDa protein existing in pearls. Evidence is presented that the sugar moieties of the protein contribute to crystal growth, starting with the nucleation step. In in vitro precipitation experiments, the 48 kDa glycoprotein of pearls directed the formation of round-shaped vaterite crystals while the 46 kDa glycoprotein of shells promoted formation of small irregular calcite particles. Furthermore, both proteins, 48 kDa/46 kDa, comprised carbonic anhydrase activity that has been implicated in CaCO3 formation. Thus, a function of the isolated glycoproteins in biomineralization is proposed together with the mechanism by which they can stabilize different calcium carbonate polymorphs.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281410
Natoli, Antonino; Wiens, Matthias; Schröder, Heinz Christoph; Stifanic, Mauro; Batel, Renato; et al.; Bio-vaterite formation by glycoproteins from freshwater pearls; Pergamon-Elsevier Science Ltd; Micron; 41; 4; 6-2010; 359-366
0968-4328
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281410
identifier_str_mv Natoli, Antonino; Wiens, Matthias; Schröder, Heinz Christoph; Stifanic, Mauro; Batel, Renato; et al.; Bio-vaterite formation by glycoproteins from freshwater pearls; Pergamon-Elsevier Science Ltd; Micron; 41; 4; 6-2010; 359-366
0968-4328
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0968432810000041
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micron.2010.01.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1867098224301441024
score 12.832306

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