Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
- Autores
- Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Jennings Gee, Jamie. Wake Forest School of Medicine; Estados Unidos
Fil: Dubey, Purnima. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Deora, Rajendar. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos - Materia
-
BIOFILMS
BORDETELLA PERTUSSIS
HYPERBIOFILM
VIRULENCE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/49437
Ver los metadatos del registro completo
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Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traitsCattelan, NataliaJennings Gee, JamieDubey, PurnimaYantorno, Osvaldo MiguelDeora, RajendarBIOFILMSBORDETELLA PERTUSSISHYPERBIOFILMVIRULENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Jennings Gee, Jamie. Wake Forest School of Medicine; Estados UnidosFil: Dubey, Purnima. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados UnidosFil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Deora, Rajendar. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados UnidosAmerican Society for Microbiology2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49437Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar; Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits; American Society for Microbiology; Infection and Immunity; 85; 12; 9-2017; 1-45; e00373-170019-9567CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/lookup/doi/10.1128/IAI.00373-17info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00373-17info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:01Zoai:ri.conicet.gov.ar:11336/49437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:02.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
title |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
spellingShingle |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits Cattelan, Natalia BIOFILMS BORDETELLA PERTUSSIS HYPERBIOFILM VIRULENCE |
title_short |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
title_full |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
title_fullStr |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
title_full_unstemmed |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
title_sort |
Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits |
dc.creator.none.fl_str_mv |
Cattelan, Natalia Jennings Gee, Jamie Dubey, Purnima Yantorno, Osvaldo Miguel Deora, Rajendar |
author |
Cattelan, Natalia |
author_facet |
Cattelan, Natalia Jennings Gee, Jamie Dubey, Purnima Yantorno, Osvaldo Miguel Deora, Rajendar |
author_role |
author |
author2 |
Jennings Gee, Jamie Dubey, Purnima Yantorno, Osvaldo Miguel Deora, Rajendar |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
BIOFILMS BORDETELLA PERTUSSIS HYPERBIOFILM VIRULENCE |
topic |
BIOFILMS BORDETELLA PERTUSSIS HYPERBIOFILM VIRULENCE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation. Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Jennings Gee, Jamie. Wake Forest School of Medicine; Estados Unidos Fil: Dubey, Purnima. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Deora, Rajendar. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos |
description |
Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/49437 Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar; Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits; American Society for Microbiology; Infection and Immunity; 85; 12; 9-2017; 1-45; e00373-17 0019-9567 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/49437 |
identifier_str_mv |
Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar; Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits; American Society for Microbiology; Infection and Immunity; 85; 12; 9-2017; 1-45; e00373-17 0019-9567 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/lookup/doi/10.1128/IAI.00373-17 info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00373-17 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |