Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits

Autores
Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Jennings Gee, Jamie. Wake Forest School of Medicine; Estados Unidos
Fil: Dubey, Purnima. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Deora, Rajendar. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos
Materia
BIOFILMS
BORDETELLA PERTUSSIS
HYPERBIOFILM
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49437
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traitsCattelan, NataliaJennings Gee, JamieDubey, PurnimaYantorno, Osvaldo MiguelDeora, RajendarBIOFILMSBORDETELLA PERTUSSISHYPERBIOFILMVIRULENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Jennings Gee, Jamie. Wake Forest School of Medicine; Estados UnidosFil: Dubey, Purnima. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados UnidosFil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Deora, Rajendar. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados UnidosAmerican Society for Microbiology2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49437Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar; Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits; American Society for Microbiology; Infection and Immunity; 85; 12; 9-2017; 1-45; e00373-170019-9567CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/lookup/doi/10.1128/IAI.00373-17info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00373-17info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:59Zoai:ri.conicet.gov.ar:11336/49437instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:59.313CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
title Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
spellingShingle Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
Cattelan, Natalia
BIOFILMS
BORDETELLA PERTUSSIS
HYPERBIOFILM
VIRULENCE
title_short Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
title_full Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
title_fullStr Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
title_full_unstemmed Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
title_sort Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits
dc.creator.none.fl_str_mv Cattelan, Natalia
Jennings Gee, Jamie
Dubey, Purnima
Yantorno, Osvaldo Miguel
Deora, Rajendar
author Cattelan, Natalia
author_facet Cattelan, Natalia
Jennings Gee, Jamie
Dubey, Purnima
Yantorno, Osvaldo Miguel
Deora, Rajendar
author_role author
author2 Jennings Gee, Jamie
Dubey, Purnima
Yantorno, Osvaldo Miguel
Deora, Rajendar
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOFILMS
BORDETELLA PERTUSSIS
HYPERBIOFILM
VIRULENCE
topic BIOFILMS
BORDETELLA PERTUSSIS
HYPERBIOFILM
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.
Fil: Cattelan, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Jennings Gee, Jamie. Wake Forest School of Medicine; Estados Unidos
Fil: Dubey, Purnima. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Deora, Rajendar. Wake Forest School of Medicine; Estados Unidos. Ohio State University; Estados Unidos
description Pertussis, or whooping cough, caused by the obligate human pathogen Bordetella pertussis is undergoing a worldwide resurgence. The majority of studies of this pathogen are conducted with laboratory-adapted strains which may not be representative of the species as a whole. Biofilm formation by B. pertussis plays an important role in pathogenesis. We conducted a side-by-side comparison of the biofilm-forming abilities of the prototype laboratory strains and the currently circulating isolates from two countries with different vaccination programs. Compared to the reference strain, all strains examined herein formed biofilms at high levels. Biofilm structural analyses revealed country-specific differences, with strains from the United States forming more structured biofilms. Bacterial hyperaggregation and reciprocal expression of biofilm-promoting and -inhibitory factors were observed in clinical isolates. An association of increased biofilm formation with augmented epithelial cell adhesion and higher levels of bacterial colonization in the mouse nose and trachea was detected. To our knowledge, this work links for the first time increased biofilm formation in bacteria with a colonization advantage in an animal model. We propose that the enhanced biofilm-forming capacity of currently circulating strains contributes to their persistence, transmission, and continued circulation.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49437
Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar; Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits; American Society for Microbiology; Infection and Immunity; 85; 12; 9-2017; 1-45; e00373-17
0019-9567
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49437
identifier_str_mv Cattelan, Natalia; Jennings Gee, Jamie; Dubey, Purnima; Yantorno, Osvaldo Miguel; Deora, Rajendar; Hyperbiofilm formation by Bordetella pertussis strains correlates with enhanced virulence traits; American Society for Microbiology; Infection and Immunity; 85; 12; 9-2017; 1-45; e00373-17
0019-9567
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/lookup/doi/10.1128/IAI.00373-17
info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00373-17
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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