Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis

Autores
Sobarzo, Cristian Marcelo; Lustig, Livia; Ponzio, Roberto Oscar; Denduchis, Berta
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigated the effect of DEHP exposure on N-cadherin and -, - and p120-catenin immunoreactivities in the rat testis. DEHP was administered by daily gavage to 25-day-old male Sprague–Dawley rats at a dose of 2 g DEHP/5 ml corn oil/kg body weight for 2 days or 7 days. Control rats were treated with corn oil vehicle under the same conditions. Animals were killed at 24 h after the last treatment. Another group of rats treated with DEHP or corn oil vehicle (control group) for 2 days were held for 30 days without treatment to observe recovery. Testes were analyzed for histopathology, TUNEL staining, immunofluorescence (IF) and Western blot analyses. Animals exposed to DEHP for 2 days or 7 days showed severe alterations of seminiferous tubules characterized by germ cell sloughing. Animals from the longer term recovery group treated with DEHP showed foci of delayed spermatogenesis. A linear and continuous pattern of N-cadherin was observed in the basal compartment of the seminiferous tubules. A similar pattern but with higher IF intensity was observed for N-cadherin in rats treated with DEHP for 2 days or 7 days, compared to control animals. The -, - and p120-catenins were detected in the basal compartment of seminiferous tubules in similar localization and IF pattern for DEHP and control groups. A significant increase in testicular N-cadherin and -catenin levels was detected by Western blot analysis in DEHP-exposed versus control rats. No variations in N-cadherin or catenin expression were detected in the recovery groups. These findings demonstrate that DEHP induces an up-regulation of N-cadherin and -catenin expression and may perturb cell–cell adhesion phenomena in the seminiferous tubule.
Fil: Sobarzo, Cristian Marcelo. Universidad Católica de Temuco; Chile. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Lustig, Livia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ponzio, Roberto Oscar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Denduchis, Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Materia
Testis
Sertoli cell toxican
Di-(2-ethylhexyl) phthalate (DEHP)
N-cadherin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242053
Acceder
id CONICETDig_e26497674292b8e7f06afff70efd7a0f
oai_identifier_str oai:ri.conicet.gov.ar:11336/242053
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testisSobarzo, Cristian MarceloLustig, LiviaPonzio, Roberto OscarDenduchis, BertaTestisSertoli cell toxicanDi-(2-ethylhexyl) phthalate (DEHP)N-cadherinThis study investigated the effect of DEHP exposure on N-cadherin and -, - and p120-catenin immunoreactivities in the rat testis. DEHP was administered by daily gavage to 25-day-old male Sprague–Dawley rats at a dose of 2 g DEHP/5 ml corn oil/kg body weight for 2 days or 7 days. Control rats were treated with corn oil vehicle under the same conditions. Animals were killed at 24 h after the last treatment. Another group of rats treated with DEHP or corn oil vehicle (control group) for 2 days were held for 30 days without treatment to observe recovery. Testes were analyzed for histopathology, TUNEL staining, immunofluorescence (IF) and Western blot analyses. Animals exposed to DEHP for 2 days or 7 days showed severe alterations of seminiferous tubules characterized by germ cell sloughing. Animals from the longer term recovery group treated with DEHP showed foci of delayed spermatogenesis. A linear and continuous pattern of N-cadherin was observed in the basal compartment of the seminiferous tubules. A similar pattern but with higher IF intensity was observed for N-cadherin in rats treated with DEHP for 2 days or 7 days, compared to control animals. The -, - and p120-catenins were detected in the basal compartment of seminiferous tubules in similar localization and IF pattern for DEHP and control groups. A significant increase in testicular N-cadherin and -catenin levels was detected by Western blot analysis in DEHP-exposed versus control rats. No variations in N-cadherin or catenin expression were detected in the recovery groups. These findings demonstrate that DEHP induces an up-regulation of N-cadherin and -catenin expression and may perturb cell–cell adhesion phenomena in the seminiferous tubule.Fil: Sobarzo, Cristian Marcelo. Universidad Católica de Temuco; Chile. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Lustig, Livia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ponzio, Roberto Oscar. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Denduchis, Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaPergamon-Elsevier Science Ltd2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242053Sobarzo, Cristian Marcelo; Lustig, Livia; Ponzio, Roberto Oscar; Denduchis, Berta; Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis; Pergamon-Elsevier Science Ltd; Reproductive Toxicology; 22; 1; 12-2006; 77-860890-6238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0890623806000591info:eu-repo/semantics/altIdentifier/doi/10.1016/j.reprotox.2006.02.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:56Zoai:ri.conicet.gov.ar:11336/242053instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:57.14CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
title Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
spellingShingle Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
Sobarzo, Cristian Marcelo
Testis
Sertoli cell toxican
Di-(2-ethylhexyl) phthalate (DEHP)
N-cadherin
title_short Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
title_full Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
title_fullStr Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
title_full_unstemmed Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
title_sort Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis
dc.creator.none.fl_str_mv Sobarzo, Cristian Marcelo
Lustig, Livia
Ponzio, Roberto Oscar
Denduchis, Berta
author Sobarzo, Cristian Marcelo
author_facet Sobarzo, Cristian Marcelo
Lustig, Livia
Ponzio, Roberto Oscar
Denduchis, Berta
author_role author
author2 Lustig, Livia
Ponzio, Roberto Oscar
Denduchis, Berta
author2_role author
author
author
dc.subject.none.fl_str_mv Testis
Sertoli cell toxican
Di-(2-ethylhexyl) phthalate (DEHP)
N-cadherin
topic Testis
Sertoli cell toxican
Di-(2-ethylhexyl) phthalate (DEHP)
N-cadherin
dc.description.none.fl_txt_mv This study investigated the effect of DEHP exposure on N-cadherin and -, - and p120-catenin immunoreactivities in the rat testis. DEHP was administered by daily gavage to 25-day-old male Sprague–Dawley rats at a dose of 2 g DEHP/5 ml corn oil/kg body weight for 2 days or 7 days. Control rats were treated with corn oil vehicle under the same conditions. Animals were killed at 24 h after the last treatment. Another group of rats treated with DEHP or corn oil vehicle (control group) for 2 days were held for 30 days without treatment to observe recovery. Testes were analyzed for histopathology, TUNEL staining, immunofluorescence (IF) and Western blot analyses. Animals exposed to DEHP for 2 days or 7 days showed severe alterations of seminiferous tubules characterized by germ cell sloughing. Animals from the longer term recovery group treated with DEHP showed foci of delayed spermatogenesis. A linear and continuous pattern of N-cadherin was observed in the basal compartment of the seminiferous tubules. A similar pattern but with higher IF intensity was observed for N-cadherin in rats treated with DEHP for 2 days or 7 days, compared to control animals. The -, - and p120-catenins were detected in the basal compartment of seminiferous tubules in similar localization and IF pattern for DEHP and control groups. A significant increase in testicular N-cadherin and -catenin levels was detected by Western blot analysis in DEHP-exposed versus control rats. No variations in N-cadherin or catenin expression were detected in the recovery groups. These findings demonstrate that DEHP induces an up-regulation of N-cadherin and -catenin expression and may perturb cell–cell adhesion phenomena in the seminiferous tubule.
Fil: Sobarzo, Cristian Marcelo. Universidad Católica de Temuco; Chile. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Lustig, Livia. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ponzio, Roberto Oscar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Denduchis, Berta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description This study investigated the effect of DEHP exposure on N-cadherin and -, - and p120-catenin immunoreactivities in the rat testis. DEHP was administered by daily gavage to 25-day-old male Sprague–Dawley rats at a dose of 2 g DEHP/5 ml corn oil/kg body weight for 2 days or 7 days. Control rats were treated with corn oil vehicle under the same conditions. Animals were killed at 24 h after the last treatment. Another group of rats treated with DEHP or corn oil vehicle (control group) for 2 days were held for 30 days without treatment to observe recovery. Testes were analyzed for histopathology, TUNEL staining, immunofluorescence (IF) and Western blot analyses. Animals exposed to DEHP for 2 days or 7 days showed severe alterations of seminiferous tubules characterized by germ cell sloughing. Animals from the longer term recovery group treated with DEHP showed foci of delayed spermatogenesis. A linear and continuous pattern of N-cadherin was observed in the basal compartment of the seminiferous tubules. A similar pattern but with higher IF intensity was observed for N-cadherin in rats treated with DEHP for 2 days or 7 days, compared to control animals. The -, - and p120-catenins were detected in the basal compartment of seminiferous tubules in similar localization and IF pattern for DEHP and control groups. A significant increase in testicular N-cadherin and -catenin levels was detected by Western blot analysis in DEHP-exposed versus control rats. No variations in N-cadherin or catenin expression were detected in the recovery groups. These findings demonstrate that DEHP induces an up-regulation of N-cadherin and -catenin expression and may perturb cell–cell adhesion phenomena in the seminiferous tubule.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242053
Sobarzo, Cristian Marcelo; Lustig, Livia; Ponzio, Roberto Oscar; Denduchis, Berta; Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis; Pergamon-Elsevier Science Ltd; Reproductive Toxicology; 22; 1; 12-2006; 77-86
0890-6238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242053
identifier_str_mv Sobarzo, Cristian Marcelo; Lustig, Livia; Ponzio, Roberto Oscar; Denduchis, Berta; Effect of di-(2-ethylhexyl) phthalate on N-cadherin and catenin protein expression in rat testis; Pergamon-Elsevier Science Ltd; Reproductive Toxicology; 22; 1; 12-2006; 77-86
0890-6238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0890623806000591
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.reprotox.2006.02.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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