A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production

Autores
Chiappini, Santiago Andrés; Kormes, Diego J.; Bonetto, Maria Celina; Sacco, Natalia Jimena; Corton, Eduardo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As aerobic respiration proceeds it consumes oxygen and produces carbon dioxide; and the relation between these two parameters, the respiratory quotient (RQ), is related to the type of substances being respired. Therefore, for a given water or wastewater origin, a more or less stable RQ is expected, making possible the estimation of BOD5 by means of CO2 production measurement. The microbial breathing intensity was continuously measured using a potentiometric CO2 electrode as transducer. We named this new biosensor BODstCO2. Proof of concept in this study was carried out with Saccharomyces cerevisiae or a BODSEED mixed microbial community as a biological recognition component. The effect of microbial load (0.5, 5, and 25 mg cm-2 membrane, dry weight) over the apparent linear range (up to 670 mg L-1 BOD5, 5 mg cm-2 membrane), detection limit (ca. 1 mg L-1 BOD5), stabilization time, reproducibility (typically better than 10%) and bio-membrane type (membrane or PVA hydrogel entrapment) were studied. When the Nernstian biosensor response was used for calibration, up to 20,000 mg L-1 glucose standard was measured without sample dilution. BOD calibrations were accomplished using the two more commonly used standard artificial wastewaters, GGA and OECD solutions. The results showed that the potentiometric CO2 electrode was an useful transducer, allowing us to build, calibrate and characterize a BOD-like biosensor. Moreover, limitations present at oxygen amperometric electrode (customarily used as BOD biosensor-based transducer) such as oxygen low solubility and its reduction at the cathode were avoided.
Fil: Chiappini, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad de Buenos Aires; Argentina
Fil: Kormes, Diego J.. Universidad de Buenos Aires; Argentina
Fil: Bonetto, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Sacco, Natalia Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Corton, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
BOD
BODSTCO2
BODSEED
CARBON DIOXIDE
POTENTIOMETRY
PVA
SACCHAROMYCES CEREVISIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211808
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/211808
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A new microbial biosensor for organic water pollution based on measurement of carbon dioxide productionChiappini, Santiago AndrésKormes, Diego J.Bonetto, Maria CelinaSacco, Natalia JimenaCorton, EduardoBODBODSTCO2BODSEEDCARBON DIOXIDEPOTENTIOMETRYPVASACCHAROMYCES CEREVISIAEhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2As aerobic respiration proceeds it consumes oxygen and produces carbon dioxide; and the relation between these two parameters, the respiratory quotient (RQ), is related to the type of substances being respired. Therefore, for a given water or wastewater origin, a more or less stable RQ is expected, making possible the estimation of BOD5 by means of CO2 production measurement. The microbial breathing intensity was continuously measured using a potentiometric CO2 electrode as transducer. We named this new biosensor BODstCO2. Proof of concept in this study was carried out with Saccharomyces cerevisiae or a BODSEED mixed microbial community as a biological recognition component. The effect of microbial load (0.5, 5, and 25 mg cm-2 membrane, dry weight) over the apparent linear range (up to 670 mg L-1 BOD5, 5 mg cm-2 membrane), detection limit (ca. 1 mg L-1 BOD5), stabilization time, reproducibility (typically better than 10%) and bio-membrane type (membrane or PVA hydrogel entrapment) were studied. When the Nernstian biosensor response was used for calibration, up to 20,000 mg L-1 glucose standard was measured without sample dilution. BOD calibrations were accomplished using the two more commonly used standard artificial wastewaters, GGA and OECD solutions. The results showed that the potentiometric CO2 electrode was an useful transducer, allowing us to build, calibrate and characterize a BOD-like biosensor. Moreover, limitations present at oxygen amperometric electrode (customarily used as BOD biosensor-based transducer) such as oxygen low solubility and its reduction at the cathode were avoided.Fil: Chiappini, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad de Buenos Aires; ArgentinaFil: Kormes, Diego J.. Universidad de Buenos Aires; ArgentinaFil: Bonetto, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Sacco, Natalia Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Corton, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaElsevier Science SA2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211808Chiappini, Santiago Andrés; Kormes, Diego J.; Bonetto, Maria Celina; Sacco, Natalia Jimena; Corton, Eduardo; A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production; Elsevier Science SA; Sensors and Actuators B: Chemical; 148; 1; 6-2010; 103-1090925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400510003837info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.04.039info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:39Zoai:ri.conicet.gov.ar:11336/211808instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:39.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
title A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
spellingShingle A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
Chiappini, Santiago Andrés
BOD
BODSTCO2
BODSEED
CARBON DIOXIDE
POTENTIOMETRY
PVA
SACCHAROMYCES CEREVISIAE
title_short A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
title_full A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
title_fullStr A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
title_full_unstemmed A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
title_sort A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production
dc.creator.none.fl_str_mv Chiappini, Santiago Andrés
Kormes, Diego J.
Bonetto, Maria Celina
Sacco, Natalia Jimena
Corton, Eduardo
author Chiappini, Santiago Andrés
author_facet Chiappini, Santiago Andrés
Kormes, Diego J.
Bonetto, Maria Celina
Sacco, Natalia Jimena
Corton, Eduardo
author_role author
author2 Kormes, Diego J.
Bonetto, Maria Celina
Sacco, Natalia Jimena
Corton, Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv BOD
BODSTCO2
BODSEED
CARBON DIOXIDE
POTENTIOMETRY
PVA
SACCHAROMYCES CEREVISIAE
topic BOD
BODSTCO2
BODSEED
CARBON DIOXIDE
POTENTIOMETRY
PVA
SACCHAROMYCES CEREVISIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv As aerobic respiration proceeds it consumes oxygen and produces carbon dioxide; and the relation between these two parameters, the respiratory quotient (RQ), is related to the type of substances being respired. Therefore, for a given water or wastewater origin, a more or less stable RQ is expected, making possible the estimation of BOD5 by means of CO2 production measurement. The microbial breathing intensity was continuously measured using a potentiometric CO2 electrode as transducer. We named this new biosensor BODstCO2. Proof of concept in this study was carried out with Saccharomyces cerevisiae or a BODSEED mixed microbial community as a biological recognition component. The effect of microbial load (0.5, 5, and 25 mg cm-2 membrane, dry weight) over the apparent linear range (up to 670 mg L-1 BOD5, 5 mg cm-2 membrane), detection limit (ca. 1 mg L-1 BOD5), stabilization time, reproducibility (typically better than 10%) and bio-membrane type (membrane or PVA hydrogel entrapment) were studied. When the Nernstian biosensor response was used for calibration, up to 20,000 mg L-1 glucose standard was measured without sample dilution. BOD calibrations were accomplished using the two more commonly used standard artificial wastewaters, GGA and OECD solutions. The results showed that the potentiometric CO2 electrode was an useful transducer, allowing us to build, calibrate and characterize a BOD-like biosensor. Moreover, limitations present at oxygen amperometric electrode (customarily used as BOD biosensor-based transducer) such as oxygen low solubility and its reduction at the cathode were avoided.
Fil: Chiappini, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad de Buenos Aires; Argentina
Fil: Kormes, Diego J.. Universidad de Buenos Aires; Argentina
Fil: Bonetto, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Sacco, Natalia Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Corton, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description As aerobic respiration proceeds it consumes oxygen and produces carbon dioxide; and the relation between these two parameters, the respiratory quotient (RQ), is related to the type of substances being respired. Therefore, for a given water or wastewater origin, a more or less stable RQ is expected, making possible the estimation of BOD5 by means of CO2 production measurement. The microbial breathing intensity was continuously measured using a potentiometric CO2 electrode as transducer. We named this new biosensor BODstCO2. Proof of concept in this study was carried out with Saccharomyces cerevisiae or a BODSEED mixed microbial community as a biological recognition component. The effect of microbial load (0.5, 5, and 25 mg cm-2 membrane, dry weight) over the apparent linear range (up to 670 mg L-1 BOD5, 5 mg cm-2 membrane), detection limit (ca. 1 mg L-1 BOD5), stabilization time, reproducibility (typically better than 10%) and bio-membrane type (membrane or PVA hydrogel entrapment) were studied. When the Nernstian biosensor response was used for calibration, up to 20,000 mg L-1 glucose standard was measured without sample dilution. BOD calibrations were accomplished using the two more commonly used standard artificial wastewaters, GGA and OECD solutions. The results showed that the potentiometric CO2 electrode was an useful transducer, allowing us to build, calibrate and characterize a BOD-like biosensor. Moreover, limitations present at oxygen amperometric electrode (customarily used as BOD biosensor-based transducer) such as oxygen low solubility and its reduction at the cathode were avoided.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211808
Chiappini, Santiago Andrés; Kormes, Diego J.; Bonetto, Maria Celina; Sacco, Natalia Jimena; Corton, Eduardo; A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production; Elsevier Science SA; Sensors and Actuators B: Chemical; 148; 1; 6-2010; 103-109
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211808
identifier_str_mv Chiappini, Santiago Andrés; Kormes, Diego J.; Bonetto, Maria Celina; Sacco, Natalia Jimena; Corton, Eduardo; A new microbial biosensor for organic water pollution based on measurement of carbon dioxide production; Elsevier Science SA; Sensors and Actuators B: Chemical; 148; 1; 6-2010; 103-109
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400510003837
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.04.039
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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