Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops

Autores
Lajmanovich, Rafael Carlos; Peltzer, Paola; Junges, Celina Maria; Attademo, Andres Maximiliano; Sanchez, Laura Cecilia; Basso, Agustin
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soybean fields provide habitats for many species of amphibians. However, the persistence and health of amphibian populations may be at risk from the increasing use of pesticides and other agricultural chemicals. We examined the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterases (CbEs) in 11 syntopic species of larval anurans. In vitro effects of malaoxon causing 50% BChE inhibition (IC50) were also studied. In addition, we calculated a relative risk index (RI) based on the geographic distributions of the anurans, the phenology of soybean cultivation, and basal enzymatic values related to potential pesticide detoxification. Among the 11 species, AChE activity varied from 17.5±1.6 to 68.2±4.7nmolmin-1mg-1 protein (PT). BChE activity also varied significantly, ranging from 3.3±0.4 to 7.5±0.4nmolmin-1mg-1PT. Both measures of CbE activities varied widely (CbE α-NA: 2.1±0.5-12.4±1.1nmolmin-1mg-1PT; CbE-4NPV: 21.8±1.8-102.6±7.9nmolmin-1mg-1PT). We also corroborate that lower BChE activity levels for the tadpoles were associated at minor IC50 values. The results of this study demonstrate significant variation in enzymatic levels among several tadpole species and intermediate to high RI values for 7 species. Based on these results, it appears that a conversion of native ecosystems to soybean crops may lead to increased ecological risk for anuran amphibians.
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Attademo, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Sanchez, Laura Cecilia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Basso, Agustin. Universidad Nacional del Litoral; Argentina
Materia
AGROECOSYSTEMS
AMPHIBIAN
B-ESTERASES
ECOLOGICAL RISK
LANDSCAPE MANAGEMENT
SOYBEAN CROPS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97706
Acceder
id CONICETDig_e2099298fa13ab55c9b4459b44cd6ec3
oai_identifier_str oai:ri.conicet.gov.ar:11336/97706
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean cropsLajmanovich, Rafael CarlosPeltzer, PaolaJunges, Celina MariaAttademo, Andres MaximilianoSanchez, Laura CeciliaBasso, AgustinAGROECOSYSTEMSAMPHIBIANB-ESTERASESECOLOGICAL RISKLANDSCAPE MANAGEMENTSOYBEAN CROPShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soybean fields provide habitats for many species of amphibians. However, the persistence and health of amphibian populations may be at risk from the increasing use of pesticides and other agricultural chemicals. We examined the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterases (CbEs) in 11 syntopic species of larval anurans. In vitro effects of malaoxon causing 50% BChE inhibition (IC50) were also studied. In addition, we calculated a relative risk index (RI) based on the geographic distributions of the anurans, the phenology of soybean cultivation, and basal enzymatic values related to potential pesticide detoxification. Among the 11 species, AChE activity varied from 17.5±1.6 to 68.2±4.7nmolmin-1mg-1 protein (PT). BChE activity also varied significantly, ranging from 3.3±0.4 to 7.5±0.4nmolmin-1mg-1PT. Both measures of CbE activities varied widely (CbE α-NA: 2.1±0.5-12.4±1.1nmolmin-1mg-1PT; CbE-4NPV: 21.8±1.8-102.6±7.9nmolmin-1mg-1PT). We also corroborate that lower BChE activity levels for the tadpoles were associated at minor IC50 values. The results of this study demonstrate significant variation in enzymatic levels among several tadpole species and intermediate to high RI values for 7 species. Based on these results, it appears that a conversion of native ecosystems to soybean crops may lead to increased ecological risk for anuran amphibians.Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Junges, Celina Maria. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Attademo, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Sanchez, Laura Cecilia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Basso, Agustin. Universidad Nacional del Litoral; ArgentinaAcademic Press Inc Elsevier Science2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97706Lajmanovich, Rafael Carlos; Peltzer, Paola; Junges, Celina Maria; Attademo, Andres Maximiliano; Sanchez, Laura Cecilia; et al.; Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 73; 7; 10-2010; 1517-15240147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2010.07.047info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:36Zoai:ri.conicet.gov.ar:11336/97706instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:36.665CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
title Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
spellingShingle Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
Lajmanovich, Rafael Carlos
AGROECOSYSTEMS
AMPHIBIAN
B-ESTERASES
ECOLOGICAL RISK
LANDSCAPE MANAGEMENT
SOYBEAN CROPS
title_short Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
title_full Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
title_fullStr Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
title_full_unstemmed Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
title_sort Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops
dc.creator.none.fl_str_mv Lajmanovich, Rafael Carlos
Peltzer, Paola
Junges, Celina Maria
Attademo, Andres Maximiliano
Sanchez, Laura Cecilia
Basso, Agustin
author Lajmanovich, Rafael Carlos
author_facet Lajmanovich, Rafael Carlos
Peltzer, Paola
Junges, Celina Maria
Attademo, Andres Maximiliano
Sanchez, Laura Cecilia
Basso, Agustin
author_role author
author2 Peltzer, Paola
Junges, Celina Maria
Attademo, Andres Maximiliano
Sanchez, Laura Cecilia
Basso, Agustin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AGROECOSYSTEMS
AMPHIBIAN
B-ESTERASES
ECOLOGICAL RISK
LANDSCAPE MANAGEMENT
SOYBEAN CROPS
topic AGROECOSYSTEMS
AMPHIBIAN
B-ESTERASES
ECOLOGICAL RISK
LANDSCAPE MANAGEMENT
SOYBEAN CROPS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Soybean fields provide habitats for many species of amphibians. However, the persistence and health of amphibian populations may be at risk from the increasing use of pesticides and other agricultural chemicals. We examined the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterases (CbEs) in 11 syntopic species of larval anurans. In vitro effects of malaoxon causing 50% BChE inhibition (IC50) were also studied. In addition, we calculated a relative risk index (RI) based on the geographic distributions of the anurans, the phenology of soybean cultivation, and basal enzymatic values related to potential pesticide detoxification. Among the 11 species, AChE activity varied from 17.5±1.6 to 68.2±4.7nmolmin-1mg-1 protein (PT). BChE activity also varied significantly, ranging from 3.3±0.4 to 7.5±0.4nmolmin-1mg-1PT. Both measures of CbE activities varied widely (CbE α-NA: 2.1±0.5-12.4±1.1nmolmin-1mg-1PT; CbE-4NPV: 21.8±1.8-102.6±7.9nmolmin-1mg-1PT). We also corroborate that lower BChE activity levels for the tadpoles were associated at minor IC50 values. The results of this study demonstrate significant variation in enzymatic levels among several tadpole species and intermediate to high RI values for 7 species. Based on these results, it appears that a conversion of native ecosystems to soybean crops may lead to increased ecological risk for anuran amphibians.
Fil: Lajmanovich, Rafael Carlos. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peltzer, Paola. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Junges, Celina Maria. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Attademo, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Sanchez, Laura Cecilia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Basso, Agustin. Universidad Nacional del Litoral; Argentina
description Soybean fields provide habitats for many species of amphibians. However, the persistence and health of amphibian populations may be at risk from the increasing use of pesticides and other agricultural chemicals. We examined the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and carboxylesterases (CbEs) in 11 syntopic species of larval anurans. In vitro effects of malaoxon causing 50% BChE inhibition (IC50) were also studied. In addition, we calculated a relative risk index (RI) based on the geographic distributions of the anurans, the phenology of soybean cultivation, and basal enzymatic values related to potential pesticide detoxification. Among the 11 species, AChE activity varied from 17.5±1.6 to 68.2±4.7nmolmin-1mg-1 protein (PT). BChE activity also varied significantly, ranging from 3.3±0.4 to 7.5±0.4nmolmin-1mg-1PT. Both measures of CbE activities varied widely (CbE α-NA: 2.1±0.5-12.4±1.1nmolmin-1mg-1PT; CbE-4NPV: 21.8±1.8-102.6±7.9nmolmin-1mg-1PT). We also corroborate that lower BChE activity levels for the tadpoles were associated at minor IC50 values. The results of this study demonstrate significant variation in enzymatic levels among several tadpole species and intermediate to high RI values for 7 species. Based on these results, it appears that a conversion of native ecosystems to soybean crops may lead to increased ecological risk for anuran amphibians.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97706
Lajmanovich, Rafael Carlos; Peltzer, Paola; Junges, Celina Maria; Attademo, Andres Maximiliano; Sanchez, Laura Cecilia; et al.; Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 73; 7; 10-2010; 1517-1524
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97706
identifier_str_mv Lajmanovich, Rafael Carlos; Peltzer, Paola; Junges, Celina Maria; Attademo, Andres Maximiliano; Sanchez, Laura Cecilia; et al.; Activity levels of B-esterases in the tadpoles of 11 species of frogs in the middle Paraná River floodplain: Implication for ecological risk assessment of soybean crops; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 73; 7; 10-2010; 1517-1524
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2010.07.047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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