Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion

Autores
Delgado, Silvia Marcela; Escudero, Carla Gimena; Casais, Marilina; Gordillo, Mauricio Miguel; Anzulovich Miranda, Ana Cecilia; Sosa, Zulema Yolanda; Rastrilla, Ana Maria
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Both peripheral innervation and nitric oxide (NO) participate in ovarian steroidogenesis. The aims of the work were (1) to investigate whether ganglionic noradrenergic (NE) and cholinergic (Ach) stimulus modify the ovarian steroids and NO release and (2) to examine the effect of those stimuli on the mRNA expression of 3β-HSD and P450 aromatase in the ovary. The experiments were carried out using the ex vivo coeliac ganglionsuperior ovarian nerveovary (CGSONO) system of rats in the first oestral cycle. The system was incubated in a buffer solution for 120 min, with the ganglion and ovary located in different compartments and linked by the SON. NE and Ach were added into the ganglion compartment. Both NE and Ach predominantly induced ovarian release of androstenedione and oestradiol while inhibited progesterone release. Ovarian NO release increased after ganglionic stimulation during proestrous while its secretion decreased during the diestrous. Noteworthily, 3β-HSD and P450 aromatase expression were modulated by neural stimulation. In the follicular phase, Ach in CG increased 3β-HSD and NE increased P450 aromatase. In the luteal phase both neurotransmitters increased 3β-HSD and Ach increased P450 aromatase transcript levels. All above observations suggest that the preponderancy of an either noradrenergic or cholinergic effect would depend on the stage of the first oestral cycle in the rat. The ovarian response to noradrenergic and cholinergic stimuli on GC, via SON, is strongly linked to oestral-stage-specific ovarian structures and their secretion products.
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Casais, Marilina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Gordillo, Mauricio Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Materia
Coeliac Ganglion
First Oestral Cycle
Ovary
P450 Aromatase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80385
Acceder
id CONICETDig_e1cb98ee48dcfb7df9000cf7c9bf6b41
oai_identifier_str oai:ri.conicet.gov.ar:11336/80385
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglionDelgado, Silvia MarcelaEscudero, Carla GimenaCasais, MarilinaGordillo, Mauricio MiguelAnzulovich Miranda, Ana CeciliaSosa, Zulema YolandaRastrilla, Ana MariaCoeliac GanglionFirst Oestral CycleOvaryP450 Aromatasehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Both peripheral innervation and nitric oxide (NO) participate in ovarian steroidogenesis. The aims of the work were (1) to investigate whether ganglionic noradrenergic (NE) and cholinergic (Ach) stimulus modify the ovarian steroids and NO release and (2) to examine the effect of those stimuli on the mRNA expression of 3β-HSD and P450 aromatase in the ovary. The experiments were carried out using the ex vivo coeliac ganglionsuperior ovarian nerveovary (CGSONO) system of rats in the first oestral cycle. The system was incubated in a buffer solution for 120 min, with the ganglion and ovary located in different compartments and linked by the SON. NE and Ach were added into the ganglion compartment. Both NE and Ach predominantly induced ovarian release of androstenedione and oestradiol while inhibited progesterone release. Ovarian NO release increased after ganglionic stimulation during proestrous while its secretion decreased during the diestrous. Noteworthily, 3β-HSD and P450 aromatase expression were modulated by neural stimulation. In the follicular phase, Ach in CG increased 3β-HSD and NE increased P450 aromatase. In the luteal phase both neurotransmitters increased 3β-HSD and Ach increased P450 aromatase transcript levels. All above observations suggest that the preponderancy of an either noradrenergic or cholinergic effect would depend on the stage of the first oestral cycle in the rat. The ovarian response to noradrenergic and cholinergic stimuli on GC, via SON, is strongly linked to oestral-stage-specific ovarian structures and their secretion products.Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Casais, Marilina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Gordillo, Mauricio Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaElsevier Science Inc2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80385Delgado, Silvia Marcela; Escudero, Carla Gimena; Casais, Marilina; Gordillo, Mauricio Miguel; Anzulovich Miranda, Ana Cecilia; et al.; Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion; Elsevier Science Inc; Steroids; 75; 10; 10-2010; 685-6940039-128XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.steroids.2010.04.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039128X10001108info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:28Zoai:ri.conicet.gov.ar:11336/80385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:28.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
title Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
spellingShingle Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
Delgado, Silvia Marcela
Coeliac Ganglion
First Oestral Cycle
Ovary
P450 Aromatase
title_short Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
title_full Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
title_fullStr Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
title_full_unstemmed Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
title_sort Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion
dc.creator.none.fl_str_mv Delgado, Silvia Marcela
Escudero, Carla Gimena
Casais, Marilina
Gordillo, Mauricio Miguel
Anzulovich Miranda, Ana Cecilia
Sosa, Zulema Yolanda
Rastrilla, Ana Maria
author Delgado, Silvia Marcela
author_facet Delgado, Silvia Marcela
Escudero, Carla Gimena
Casais, Marilina
Gordillo, Mauricio Miguel
Anzulovich Miranda, Ana Cecilia
Sosa, Zulema Yolanda
Rastrilla, Ana Maria
author_role author
author2 Escudero, Carla Gimena
Casais, Marilina
Gordillo, Mauricio Miguel
Anzulovich Miranda, Ana Cecilia
Sosa, Zulema Yolanda
Rastrilla, Ana Maria
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Coeliac Ganglion
First Oestral Cycle
Ovary
P450 Aromatase
topic Coeliac Ganglion
First Oestral Cycle
Ovary
P450 Aromatase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Both peripheral innervation and nitric oxide (NO) participate in ovarian steroidogenesis. The aims of the work were (1) to investigate whether ganglionic noradrenergic (NE) and cholinergic (Ach) stimulus modify the ovarian steroids and NO release and (2) to examine the effect of those stimuli on the mRNA expression of 3β-HSD and P450 aromatase in the ovary. The experiments were carried out using the ex vivo coeliac ganglionsuperior ovarian nerveovary (CGSONO) system of rats in the first oestral cycle. The system was incubated in a buffer solution for 120 min, with the ganglion and ovary located in different compartments and linked by the SON. NE and Ach were added into the ganglion compartment. Both NE and Ach predominantly induced ovarian release of androstenedione and oestradiol while inhibited progesterone release. Ovarian NO release increased after ganglionic stimulation during proestrous while its secretion decreased during the diestrous. Noteworthily, 3β-HSD and P450 aromatase expression were modulated by neural stimulation. In the follicular phase, Ach in CG increased 3β-HSD and NE increased P450 aromatase. In the luteal phase both neurotransmitters increased 3β-HSD and Ach increased P450 aromatase transcript levels. All above observations suggest that the preponderancy of an either noradrenergic or cholinergic effect would depend on the stage of the first oestral cycle in the rat. The ovarian response to noradrenergic and cholinergic stimuli on GC, via SON, is strongly linked to oestral-stage-specific ovarian structures and their secretion products.
Fil: Delgado, Silvia Marcela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Escudero, Carla Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Casais, Marilina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Gordillo, Mauricio Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
description Both peripheral innervation and nitric oxide (NO) participate in ovarian steroidogenesis. The aims of the work were (1) to investigate whether ganglionic noradrenergic (NE) and cholinergic (Ach) stimulus modify the ovarian steroids and NO release and (2) to examine the effect of those stimuli on the mRNA expression of 3β-HSD and P450 aromatase in the ovary. The experiments were carried out using the ex vivo coeliac ganglionsuperior ovarian nerveovary (CGSONO) system of rats in the first oestral cycle. The system was incubated in a buffer solution for 120 min, with the ganglion and ovary located in different compartments and linked by the SON. NE and Ach were added into the ganglion compartment. Both NE and Ach predominantly induced ovarian release of androstenedione and oestradiol while inhibited progesterone release. Ovarian NO release increased after ganglionic stimulation during proestrous while its secretion decreased during the diestrous. Noteworthily, 3β-HSD and P450 aromatase expression were modulated by neural stimulation. In the follicular phase, Ach in CG increased 3β-HSD and NE increased P450 aromatase. In the luteal phase both neurotransmitters increased 3β-HSD and Ach increased P450 aromatase transcript levels. All above observations suggest that the preponderancy of an either noradrenergic or cholinergic effect would depend on the stage of the first oestral cycle in the rat. The ovarian response to noradrenergic and cholinergic stimuli on GC, via SON, is strongly linked to oestral-stage-specific ovarian structures and their secretion products.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80385
Delgado, Silvia Marcela; Escudero, Carla Gimena; Casais, Marilina; Gordillo, Mauricio Miguel; Anzulovich Miranda, Ana Cecilia; et al.; Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion; Elsevier Science Inc; Steroids; 75; 10; 10-2010; 685-694
0039-128X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80385
identifier_str_mv Delgado, Silvia Marcela; Escudero, Carla Gimena; Casais, Marilina; Gordillo, Mauricio Miguel; Anzulovich Miranda, Ana Cecilia; et al.; Ovaric physiology in the first oestral cycle: Influence of noradrenergic and cholinergic neural stimuli from coeliac ganglion; Elsevier Science Inc; Steroids; 75; 10; 10-2010; 685-694
0039-128X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.steroids.2010.04.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039128X10001108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269463673569280
score 13.13397

Publicaciones similares