Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment

Autores
Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; Bosch, Carme; Verdum, Maria; Jove, Patricia; de Pablo, Joan; Morató, Jordi
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth.
Fil: Aguilar, Lorena. Universidad Politécnica de Catalunya; España
Fil: Perez, Leonardo Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería-Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Gallegos, Ángel. Universidad Politécnica de Catalunya; España
Fil: Fores, Eva. Universidad Politécnica de Catalunya; España
Fil: Arias, Carlos A.. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Bosch, Carme. No especifíca;
Fil: Verdum, Maria. No especifíca;
Fil: Jove, Patricia. No especifíca;
Fil: de Pablo, Joan. Universidad Politécnica de Catalunya; España
Fil: Morató, Jordi. Universidad Politécnica de Catalunya; España
Materia
AERATION
CORK
DENITRIFYING BACTERIA
NITRATE POLLUTION
VERTICAL-FLOW
WINERY WASTEWATER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217754
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/217754
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatmentAguilar, LorenaPerez, Leonardo MartinGallegos, ÁngelFores, EvaArias, Carlos A.Bosch, CarmeVerdum, MariaJove, Patriciade Pablo, JoanMorató, JordiAERATIONCORKDENITRIFYING BACTERIANITRATE POLLUTIONVERTICAL-FLOWWINERY WASTEWATERhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth.Fil: Aguilar, Lorena. Universidad Politécnica de Catalunya; EspañaFil: Perez, Leonardo Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería-Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Gallegos, Ángel. Universidad Politécnica de Catalunya; EspañaFil: Fores, Eva. Universidad Politécnica de Catalunya; EspañaFil: Arias, Carlos A.. Aarhus University. Aarhus Institute Of Advanced Studies.; DinamarcaFil: Bosch, Carme. No especifíca;Fil: Verdum, Maria. No especifíca;Fil: Jove, Patricia. No especifíca;Fil: de Pablo, Joan. Universidad Politécnica de Catalunya; EspañaFil: Morató, Jordi. Universidad Politécnica de Catalunya; EspañaElsevier Science2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217754Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; et al.; Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment; Elsevier Science; Ecological Engineering; 185; 12-2022; 1-70925-8574CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925857422002427info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoleng.2022.106781info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:12Zoai:ri.conicet.gov.ar:11336/217754instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:13.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
title Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
spellingShingle Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
Aguilar, Lorena
AERATION
CORK
DENITRIFYING BACTERIA
NITRATE POLLUTION
VERTICAL-FLOW
WINERY WASTEWATER
title_short Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
title_full Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
title_fullStr Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
title_full_unstemmed Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
title_sort Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
dc.creator.none.fl_str_mv Aguilar, Lorena
Perez, Leonardo Martin
Gallegos, Ángel
Fores, Eva
Arias, Carlos A.
Bosch, Carme
Verdum, Maria
Jove, Patricia
de Pablo, Joan
Morató, Jordi
author Aguilar, Lorena
author_facet Aguilar, Lorena
Perez, Leonardo Martin
Gallegos, Ángel
Fores, Eva
Arias, Carlos A.
Bosch, Carme
Verdum, Maria
Jove, Patricia
de Pablo, Joan
Morató, Jordi
author_role author
author2 Perez, Leonardo Martin
Gallegos, Ángel
Fores, Eva
Arias, Carlos A.
Bosch, Carme
Verdum, Maria
Jove, Patricia
de Pablo, Joan
Morató, Jordi
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AERATION
CORK
DENITRIFYING BACTERIA
NITRATE POLLUTION
VERTICAL-FLOW
WINERY WASTEWATER
topic AERATION
CORK
DENITRIFYING BACTERIA
NITRATE POLLUTION
VERTICAL-FLOW
WINERY WASTEWATER
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth.
Fil: Aguilar, Lorena. Universidad Politécnica de Catalunya; España
Fil: Perez, Leonardo Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería-Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Gallegos, Ángel. Universidad Politécnica de Catalunya; España
Fil: Fores, Eva. Universidad Politécnica de Catalunya; España
Fil: Arias, Carlos A.. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Bosch, Carme. No especifíca;
Fil: Verdum, Maria. No especifíca;
Fil: Jove, Patricia. No especifíca;
Fil: de Pablo, Joan. Universidad Politécnica de Catalunya; España
Fil: Morató, Jordi. Universidad Politécnica de Catalunya; España
description The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217754
Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; et al.; Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment; Elsevier Science; Ecological Engineering; 185; 12-2022; 1-7
0925-8574
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217754
identifier_str_mv Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; et al.; Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment; Elsevier Science; Ecological Engineering; 185; 12-2022; 1-7
0925-8574
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925857422002427
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoleng.2022.106781
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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