Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment
- Autores
- Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; Bosch, Carme; Verdum, Maria; Jove, Patricia; de Pablo, Joan; Morató, Jordi
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth.
Fil: Aguilar, Lorena. Universidad Politécnica de Catalunya; España
Fil: Perez, Leonardo Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería-Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Gallegos, Ángel. Universidad Politécnica de Catalunya; España
Fil: Fores, Eva. Universidad Politécnica de Catalunya; España
Fil: Arias, Carlos A.. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca
Fil: Bosch, Carme. No especifíca;
Fil: Verdum, Maria. No especifíca;
Fil: Jove, Patricia. No especifíca;
Fil: de Pablo, Joan. Universidad Politécnica de Catalunya; España
Fil: Morató, Jordi. Universidad Politécnica de Catalunya; España - Materia
-
AERATION
CORK
DENITRIFYING BACTERIA
NITRATE POLLUTION
VERTICAL-FLOW
WINERY WASTEWATER - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/217754
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Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatmentAguilar, LorenaPerez, Leonardo MartinGallegos, ÁngelFores, EvaArias, Carlos A.Bosch, CarmeVerdum, MariaJove, Patriciade Pablo, JoanMorató, JordiAERATIONCORKDENITRIFYING BACTERIANITRATE POLLUTIONVERTICAL-FLOWWINERY WASTEWATERhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth.Fil: Aguilar, Lorena. Universidad Politécnica de Catalunya; EspañaFil: Perez, Leonardo Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería-Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Gallegos, Ángel. Universidad Politécnica de Catalunya; EspañaFil: Fores, Eva. Universidad Politécnica de Catalunya; EspañaFil: Arias, Carlos A.. Aarhus University. Aarhus Institute Of Advanced Studies.; DinamarcaFil: Bosch, Carme. No especifíca;Fil: Verdum, Maria. No especifíca;Fil: Jove, Patricia. No especifíca;Fil: de Pablo, Joan. Universidad Politécnica de Catalunya; EspañaFil: Morató, Jordi. Universidad Politécnica de Catalunya; EspañaElsevier Science2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217754Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; et al.; Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment; Elsevier Science; Ecological Engineering; 185; 12-2022; 1-70925-8574CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925857422002427info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoleng.2022.106781info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:58Zoai:ri.conicet.gov.ar:11336/217754instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:58.529CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
title |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
spellingShingle |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment Aguilar, Lorena AERATION CORK DENITRIFYING BACTERIA NITRATE POLLUTION VERTICAL-FLOW WINERY WASTEWATER |
title_short |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
title_full |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
title_fullStr |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
title_full_unstemmed |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
title_sort |
Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment |
dc.creator.none.fl_str_mv |
Aguilar, Lorena Perez, Leonardo Martin Gallegos, Ángel Fores, Eva Arias, Carlos A. Bosch, Carme Verdum, Maria Jove, Patricia de Pablo, Joan Morató, Jordi |
author |
Aguilar, Lorena |
author_facet |
Aguilar, Lorena Perez, Leonardo Martin Gallegos, Ángel Fores, Eva Arias, Carlos A. Bosch, Carme Verdum, Maria Jove, Patricia de Pablo, Joan Morató, Jordi |
author_role |
author |
author2 |
Perez, Leonardo Martin Gallegos, Ángel Fores, Eva Arias, Carlos A. Bosch, Carme Verdum, Maria Jove, Patricia de Pablo, Joan Morató, Jordi |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
AERATION CORK DENITRIFYING BACTERIA NITRATE POLLUTION VERTICAL-FLOW WINERY WASTEWATER |
topic |
AERATION CORK DENITRIFYING BACTERIA NITRATE POLLUTION VERTICAL-FLOW WINERY WASTEWATER |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth. Fil: Aguilar, Lorena. Universidad Politécnica de Catalunya; España Fil: Perez, Leonardo Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería-Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina Fil: Gallegos, Ángel. Universidad Politécnica de Catalunya; España Fil: Fores, Eva. Universidad Politécnica de Catalunya; España Fil: Arias, Carlos A.. Aarhus University. Aarhus Institute Of Advanced Studies.; Dinamarca Fil: Bosch, Carme. No especifíca; Fil: Verdum, Maria. No especifíca; Fil: Jove, Patricia. No especifíca; Fil: de Pablo, Joan. Universidad Politécnica de Catalunya; España Fil: Morató, Jordi. Universidad Politécnica de Catalunya; España |
description |
The wine industry produces large quantities of wastewater that often contains high levels of organic matter and nutrients, like nitrogen and phosphorous. In particular, nitrogen pollution can be harmful, even at low levels, since infants are vulnerable to nitrates in drinking water, and excess nitrogen can harm the health of soils and waterways. Herein, an innovative compact, modular, and mobile treatment wetland (TW) using cork by product as the only granular media was used to treat the final effluents from the Codorniu winery (Sant Sadurní d'Anoia, Spain). The TW was operated during two 5-month periods with or without intermittent induced aeration (40 min cycles, 90 L/min air flow rate). Nitrate (N-NO3) and total nitrogen (TN) removal were higher (52.8% and 46.8%, respectively, p < 0.05) during TW operation under aerated-condition. Additionally, qPCR analysis of 16S rRNA, nirS and nosZ genes revealed that intermittent induced aeration facilitates N-NO3 reduction by the stimulation of denitrifying bacteria in the TW biofilm (11.4% increase in nirS copies number/g cork sample, p < 0.05) as well as increasing the number of heterotrophic bacteria adhered to cork (25.5% increase in 16S rRNA copies number/g cork sample, p < 0.05). Moreover, SEM images demonstrated the suitability of cork as a resistant filter media for TW after long-term system operation (1.5 years). In conclusion, our results suggest that aeration improved nitrogen compounds removal compared to the non-aerated period, without affecting phosphorous elimination. Additionally, residual cork is presented here in a circular bioeconomy view, as a suitable filling media to treat winery wastewater that can provide additional carbon source to increase C/N rate stimulating denitrification, as well as a reliable organic substrate for biomass growth. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/217754 Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; et al.; Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment; Elsevier Science; Ecological Engineering; 185; 12-2022; 1-7 0925-8574 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/217754 |
identifier_str_mv |
Aguilar, Lorena; Perez, Leonardo Martin; Gallegos, Ángel; Fores, Eva; Arias, Carlos A.; et al.; Effect of aeration on nitrogen removal-associated microbial community in an innovative vertical cork-based constructed wetland for winery wastewater treatment; Elsevier Science; Ecological Engineering; 185; 12-2022; 1-7 0925-8574 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925857422002427 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoleng.2022.106781 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |