Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio

Autores
Perez, Federico; Hujer, Andrea M.; Marshall, Steven H.; Ray, Amy, J.; Rather, Philip N.; Suwantaratf, Nuntra; Dumford III, Donald; O'Shea, Patrick; Domitrovic, T. Nicholas J.; Salata, Robert A.; Chavda, Kalyan D.; Chen, Liang; Kreiswirth, Barry N; Vila, Alejandro Jose; Haussler, Susanne; Jacobs, Michael R.; Bonomo, Robert A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Carbapenems are a mainstay of treatment for infections caused by Pseudomonas aeruginosa. Carbapenem resistance mediated by metallo-β-lactamases (MBLs) remains uncommon in the United States, despite the worldwide emergence of this group of enzymes. Between March 2012 and May 2013, we detected MBL-producing P. aeruginosa in a university-affiliated health care system in northeast Ohio. We examined the clinical characteristics and outcomes of patients, defined the resistance determinants and structure of the genetic element harboring the blaMBL gene through genome sequencing, and typed MBL-producing P. aeruginosa isolates using pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR (rep-PCR), and multilocus sequence typing (MLST). Seven patients were affected that were hospitalized at three community hospitals, a long-term-care facility, and a tertiary care center; one of the patients died as a result of infection. Isolates belonged to sequence type 233 (ST233) and were extensively drug resistant (XDR), including resistance to all fluoroquinolones, aminoglycosides, and β-lactams; two isolates were nonsusceptible to colistin. The blaMBL gene was identified as blaVIM-2 contained within a class 1 integron (In559), similar to the cassette array previously detected in isolates from Norway, Russia, Taiwan, and Chicago, IL. Genomic sequencing and assembly revealed that In559 was part of a novel 35-kb region that also included a Tn501-like transposon and Salmonella genomic island 2 (SGI2)-homologous sequences. This analysis of XDR strains producing VIM-2 from northeast Ohio revealed a novel recombination event between Salmonella and P. aeruginosa, heralding a new antibiotic resistance threat in this region's health care system
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Fil: Hujer, Andrea M.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Fil: Marshall, Steven H.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos
Fil: Ray, Amy, J.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: Rather, Philip N.. Emory University School of Medicine. Atlanta; Estados Unidos. Department of Veterans Affairs Medical Center. Decatur; Estados Unidos
Fil: Suwantaratf, Nuntra. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: Dumford III, Donald. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: O'Shea, Patrick. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos
Fil: Domitrovic, T. Nicholas J.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos
Fil: Salata, Robert A.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: Chavda, Kalyan D.. Rutgers University; Estados Unidos
Fil: Chen, Liang. Rutgers University; Estados Unidos
Fil: Kreiswirth, Barry N. Rutgers University; Estados Unidos
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Haussler, Susanne. Hannover Medical School and Helmholtz Center for Infection Research; Alemania
Fil: Jacobs, Michael R.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Materia
Carbapenem
antibiotic resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29432
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/29432
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast OhioPerez, FedericoHujer, Andrea M.Marshall, Steven H.Ray, Amy, J.Rather, Philip N.Suwantaratf, NuntraDumford III, DonaldO'Shea, PatrickDomitrovic, T. Nicholas J.Salata, Robert A.Chavda, Kalyan D.Chen, LiangKreiswirth, Barry NVila, Alejandro JoseHaussler, SusanneJacobs, Michael R.Bonomo, Robert A.Carbapenemantibiotic resistancehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Carbapenems are a mainstay of treatment for infections caused by Pseudomonas aeruginosa. Carbapenem resistance mediated by metallo-β-lactamases (MBLs) remains uncommon in the United States, despite the worldwide emergence of this group of enzymes. Between March 2012 and May 2013, we detected MBL-producing P. aeruginosa in a university-affiliated health care system in northeast Ohio. We examined the clinical characteristics and outcomes of patients, defined the resistance determinants and structure of the genetic element harboring the blaMBL gene through genome sequencing, and typed MBL-producing P. aeruginosa isolates using pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR (rep-PCR), and multilocus sequence typing (MLST). Seven patients were affected that were hospitalized at three community hospitals, a long-term-care facility, and a tertiary care center; one of the patients died as a result of infection. Isolates belonged to sequence type 233 (ST233) and were extensively drug resistant (XDR), including resistance to all fluoroquinolones, aminoglycosides, and β-lactams; two isolates were nonsusceptible to colistin. The blaMBL gene was identified as blaVIM-2 contained within a class 1 integron (In559), similar to the cassette array previously detected in isolates from Norway, Russia, Taiwan, and Chicago, IL. Genomic sequencing and assembly revealed that In559 was part of a novel 35-kb region that also included a Tn501-like transposon and Salmonella genomic island 2 (SGI2)-homologous sequences. This analysis of XDR strains producing VIM-2 from northeast Ohio revealed a novel recombination event between Salmonella and P. aeruginosa, heralding a new antibiotic resistance threat in this region's health care systemFil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados UnidosFil: Hujer, Andrea M.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados UnidosFil: Marshall, Steven H.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados UnidosFil: Ray, Amy, J.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos. University Hospitals Case Medical Center. Cleveland; Estados UnidosFil: Rather, Philip N.. Emory University School of Medicine. Atlanta; Estados Unidos. Department of Veterans Affairs Medical Center. Decatur; Estados UnidosFil: Suwantaratf, Nuntra. University Hospitals Case Medical Center. Cleveland; Estados UnidosFil: Dumford III, Donald. University Hospitals Case Medical Center. Cleveland; Estados UnidosFil: O'Shea, Patrick. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados UnidosFil: Domitrovic, T. Nicholas J.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados UnidosFil: Salata, Robert A.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos. University Hospitals Case Medical Center. Cleveland; Estados UnidosFil: Chavda, Kalyan D.. Rutgers University; Estados UnidosFil: Chen, Liang. Rutgers University; Estados UnidosFil: Kreiswirth, Barry N. Rutgers University; Estados UnidosFil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Haussler, Susanne. Hannover Medical School and Helmholtz Center for Infection Research; AlemaniaFil: Jacobs, Michael R.. Case Western Reserve University School of Medicine. Cleveland; Estados UnidosFil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados UnidosAmerican Society For Microbiology2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29432Perez, Federico; Hujer, Andrea M.; Marshall, Steven H.; Ray, Amy, J.; Rather, Philip N.; et al.; Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 58; 10; 10-2014; 5929-59350066-4804CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/58/10/5929info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.02372-14info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:11Zoai:ri.conicet.gov.ar:11336/29432instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:11.765CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
title Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
spellingShingle Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
Perez, Federico
Carbapenem
antibiotic resistance
title_short Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
title_full Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
title_fullStr Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
title_full_unstemmed Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
title_sort Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio
dc.creator.none.fl_str_mv Perez, Federico
Hujer, Andrea M.
Marshall, Steven H.
Ray, Amy, J.
Rather, Philip N.
Suwantaratf, Nuntra
Dumford III, Donald
O'Shea, Patrick
Domitrovic, T. Nicholas J.
Salata, Robert A.
Chavda, Kalyan D.
Chen, Liang
Kreiswirth, Barry N
Vila, Alejandro Jose
Haussler, Susanne
Jacobs, Michael R.
Bonomo, Robert A.
author Perez, Federico
author_facet Perez, Federico
Hujer, Andrea M.
Marshall, Steven H.
Ray, Amy, J.
Rather, Philip N.
Suwantaratf, Nuntra
Dumford III, Donald
O'Shea, Patrick
Domitrovic, T. Nicholas J.
Salata, Robert A.
Chavda, Kalyan D.
Chen, Liang
Kreiswirth, Barry N
Vila, Alejandro Jose
Haussler, Susanne
Jacobs, Michael R.
Bonomo, Robert A.
author_role author
author2 Hujer, Andrea M.
Marshall, Steven H.
Ray, Amy, J.
Rather, Philip N.
Suwantaratf, Nuntra
Dumford III, Donald
O'Shea, Patrick
Domitrovic, T. Nicholas J.
Salata, Robert A.
Chavda, Kalyan D.
Chen, Liang
Kreiswirth, Barry N
Vila, Alejandro Jose
Haussler, Susanne
Jacobs, Michael R.
Bonomo, Robert A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Carbapenem
antibiotic resistance
topic Carbapenem
antibiotic resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Carbapenems are a mainstay of treatment for infections caused by Pseudomonas aeruginosa. Carbapenem resistance mediated by metallo-β-lactamases (MBLs) remains uncommon in the United States, despite the worldwide emergence of this group of enzymes. Between March 2012 and May 2013, we detected MBL-producing P. aeruginosa in a university-affiliated health care system in northeast Ohio. We examined the clinical characteristics and outcomes of patients, defined the resistance determinants and structure of the genetic element harboring the blaMBL gene through genome sequencing, and typed MBL-producing P. aeruginosa isolates using pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR (rep-PCR), and multilocus sequence typing (MLST). Seven patients were affected that were hospitalized at three community hospitals, a long-term-care facility, and a tertiary care center; one of the patients died as a result of infection. Isolates belonged to sequence type 233 (ST233) and were extensively drug resistant (XDR), including resistance to all fluoroquinolones, aminoglycosides, and β-lactams; two isolates were nonsusceptible to colistin. The blaMBL gene was identified as blaVIM-2 contained within a class 1 integron (In559), similar to the cassette array previously detected in isolates from Norway, Russia, Taiwan, and Chicago, IL. Genomic sequencing and assembly revealed that In559 was part of a novel 35-kb region that also included a Tn501-like transposon and Salmonella genomic island 2 (SGI2)-homologous sequences. This analysis of XDR strains producing VIM-2 from northeast Ohio revealed a novel recombination event between Salmonella and P. aeruginosa, heralding a new antibiotic resistance threat in this region's health care system
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Fil: Hujer, Andrea M.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Fil: Marshall, Steven H.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos
Fil: Ray, Amy, J.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: Rather, Philip N.. Emory University School of Medicine. Atlanta; Estados Unidos. Department of Veterans Affairs Medical Center. Decatur; Estados Unidos
Fil: Suwantaratf, Nuntra. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: Dumford III, Donald. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: O'Shea, Patrick. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos
Fil: Domitrovic, T. Nicholas J.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos
Fil: Salata, Robert A.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos. University Hospitals Case Medical Center. Cleveland; Estados Unidos
Fil: Chavda, Kalyan D.. Rutgers University; Estados Unidos
Fil: Chen, Liang. Rutgers University; Estados Unidos
Fil: Kreiswirth, Barry N. Rutgers University; Estados Unidos
Fil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Haussler, Susanne. Hannover Medical School and Helmholtz Center for Infection Research; Alemania
Fil: Jacobs, Michael R.. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Cleveland; Estados Unidos. Case Western Reserve University School of Medicine. Cleveland; Estados Unidos
description Carbapenems are a mainstay of treatment for infections caused by Pseudomonas aeruginosa. Carbapenem resistance mediated by metallo-β-lactamases (MBLs) remains uncommon in the United States, despite the worldwide emergence of this group of enzymes. Between March 2012 and May 2013, we detected MBL-producing P. aeruginosa in a university-affiliated health care system in northeast Ohio. We examined the clinical characteristics and outcomes of patients, defined the resistance determinants and structure of the genetic element harboring the blaMBL gene through genome sequencing, and typed MBL-producing P. aeruginosa isolates using pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR (rep-PCR), and multilocus sequence typing (MLST). Seven patients were affected that were hospitalized at three community hospitals, a long-term-care facility, and a tertiary care center; one of the patients died as a result of infection. Isolates belonged to sequence type 233 (ST233) and were extensively drug resistant (XDR), including resistance to all fluoroquinolones, aminoglycosides, and β-lactams; two isolates were nonsusceptible to colistin. The blaMBL gene was identified as blaVIM-2 contained within a class 1 integron (In559), similar to the cassette array previously detected in isolates from Norway, Russia, Taiwan, and Chicago, IL. Genomic sequencing and assembly revealed that In559 was part of a novel 35-kb region that also included a Tn501-like transposon and Salmonella genomic island 2 (SGI2)-homologous sequences. This analysis of XDR strains producing VIM-2 from northeast Ohio revealed a novel recombination event between Salmonella and P. aeruginosa, heralding a new antibiotic resistance threat in this region's health care system
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29432
Perez, Federico; Hujer, Andrea M.; Marshall, Steven H.; Ray, Amy, J.; Rather, Philip N.; et al.; Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 58; 10; 10-2014; 5929-5935
0066-4804
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29432
identifier_str_mv Perez, Federico; Hujer, Andrea M.; Marshall, Steven H.; Ray, Amy, J.; Rather, Philip N.; et al.; Extensively Drug-Resistant Pseudomonas aeruginosa Isolates Containing blaVIM-2 and Elements of Salmonella Genomic Island 2: a New Genetic Resistance Determinant in Northeast Ohio; American Society For Microbiology; Antimicrobial Agents And Chemotherapy; 58; 10; 10-2014; 5929-5935
0066-4804
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://aac.asm.org/content/58/10/5929
info:eu-repo/semantics/altIdentifier/doi/10.1128/AAC.02372-14
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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