Phenotype and functional alterations of human NK cells by organophosphate pesticides
- Autores
- Friedrich, Adrián David; Sierra, Jessica Mariel; Regge, María Victoria; Santilli, Maria Cecilia; Trotta, Aldana; Secchiari, Florencia; Domaica, Carolina Ines; Fuertes, Mercedes Beatriz; Zwirner, Norberto Walter
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Chlorpyrifos (CPF) and Glyphosate (GLY) are organophosphate pesticides widely used in agriculture. Several evidences suggest that CPF- and GLY-based pesticides are genotoxic and their use has been linked to the increased frequency of malignancies observed in highly fumigated rural areas. However, their effect on the immune system, including cells involved in immunosurveillance of tumor cells, has been poorly explored. Here, we investigated the effects of two commercial formulations containing CPF (Clorpi48) and GLY (Roundup Plus) as well as their isolated active principles on the phenotype and function of human NK cells. First, we identified sub-apoptotic doses of these substances by flow cytometry performing a dose-response curve with human peripheral blood mononuclear cells (PMBC). Next, using such sub-apoptotic doses, we analyzed the phenotype of pesticide-treated NK cells. We observed a pesticide dose-dependent reduction in the expression of CD16 and CD62L on NK cells after a 24 h of culture with CPF, GLY, Roundup and Clorpi48. Moreover, a diminished frequency of IFN--producing NK cells was observed upon exposure of cytokine-stimulated NK cells to 5 mM GLY, 0.05 mM Roundup, 0.01 mM Clorpi48 but not to 0.02 mM CPF (p<0.05). Moreover, NK cell-mediated cytotoxicity against K562 cells was also affected by pesticide treatment. After 0.05 mM Roundup and 0.01 mM Clorpi48 treatment, cytotoxicity was reduced by 27% (p<0.01) and 29% (p<0.001), respectively. In accordance with previous scientific evidence, final formulations of pesticides (which include additional compounds such as polyethoxylated alkylamines surfactants that facilitate their absorption by cells and tissues) showed a more potent effect on phenotype and function of NK cells than the isolated compounds. Therefore, we conclude that GLY- and CPF-based pesticides affect NK phenotype and function, which might impact on their ability to detect and eliminate nascent tumor cells.
Fil: Friedrich, Adrián David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Bioquímica y Farmacia. Cátedra de Inmunología; Argentina
Fil: Sierra, Jessica Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Regge, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Santilli, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Trotta, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Secchiari, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Domaica, Carolina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Fuertes, Mercedes Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Zwirner, Norberto Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Annual Meeting of Bioscience Societies 2021: LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC), LXIX Annual Meeting of Sociedad Argentina de Inmunología(SAI), LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE), XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-Ar)
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clinica
Sociedad Argentina de Inmunología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicina - Materia
-
ORGANOPHOSPHATE PESTICIDES
NK CELLS
CYTOTOXICITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/152182
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Phenotype and functional alterations of human NK cells by organophosphate pesticidesFriedrich, Adrián DavidSierra, Jessica MarielRegge, María VictoriaSantilli, Maria CeciliaTrotta, AldanaSecchiari, FlorenciaDomaica, Carolina InesFuertes, Mercedes BeatrizZwirner, Norberto WalterORGANOPHOSPHATE PESTICIDESNK CELLSCYTOTOXICITYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chlorpyrifos (CPF) and Glyphosate (GLY) are organophosphate pesticides widely used in agriculture. Several evidences suggest that CPF- and GLY-based pesticides are genotoxic and their use has been linked to the increased frequency of malignancies observed in highly fumigated rural areas. However, their effect on the immune system, including cells involved in immunosurveillance of tumor cells, has been poorly explored. Here, we investigated the effects of two commercial formulations containing CPF (Clorpi48) and GLY (Roundup Plus) as well as their isolated active principles on the phenotype and function of human NK cells. First, we identified sub-apoptotic doses of these substances by flow cytometry performing a dose-response curve with human peripheral blood mononuclear cells (PMBC). Next, using such sub-apoptotic doses, we analyzed the phenotype of pesticide-treated NK cells. We observed a pesticide dose-dependent reduction in the expression of CD16 and CD62L on NK cells after a 24 h of culture with CPF, GLY, Roundup and Clorpi48. Moreover, a diminished frequency of IFN--producing NK cells was observed upon exposure of cytokine-stimulated NK cells to 5 mM GLY, 0.05 mM Roundup, 0.01 mM Clorpi48 but not to 0.02 mM CPF (p<0.05). Moreover, NK cell-mediated cytotoxicity against K562 cells was also affected by pesticide treatment. After 0.05 mM Roundup and 0.01 mM Clorpi48 treatment, cytotoxicity was reduced by 27% (p<0.01) and 29% (p<0.001), respectively. In accordance with previous scientific evidence, final formulations of pesticides (which include additional compounds such as polyethoxylated alkylamines surfactants that facilitate their absorption by cells and tissues) showed a more potent effect on phenotype and function of NK cells than the isolated compounds. Therefore, we conclude that GLY- and CPF-based pesticides affect NK phenotype and function, which might impact on their ability to detect and eliminate nascent tumor cells.Fil: Friedrich, Adrián David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Bioquímica y Farmacia. Cátedra de Inmunología; ArgentinaFil: Sierra, Jessica Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Regge, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Santilli, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Trotta, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Secchiari, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Domaica, Carolina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Fuertes, Mercedes Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Zwirner, Norberto Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaAnnual Meeting of Bioscience Societies 2021: LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC), LXIX Annual Meeting of Sociedad Argentina de Inmunología(SAI), LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE), XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-Ar)Buenos AiresArgentinaSociedad Argentina de Investigación ClinicaSociedad Argentina de InmunologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinaFundación Revista MedicinaCurino, Alejandro CarlosMaccioni. MarianaSchaiquevich, PaulaDuran, Hebe Alicia2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152182Phenotype and functional alterations of human NK cells by organophosphate pesticides; Annual Meeting of Bioscience Societies 2021: LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC), LXIX Annual Meeting of Sociedad Argentina de Inmunología(SAI), LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE), XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-Ar); Buenos Aires; Argentina; 2021; 138-1381669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:30Zoai:ri.conicet.gov.ar:11336/152182instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:30.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
title |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
spellingShingle |
Phenotype and functional alterations of human NK cells by organophosphate pesticides Friedrich, Adrián David ORGANOPHOSPHATE PESTICIDES NK CELLS CYTOTOXICITY |
title_short |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
title_full |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
title_fullStr |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
title_full_unstemmed |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
title_sort |
Phenotype and functional alterations of human NK cells by organophosphate pesticides |
dc.creator.none.fl_str_mv |
Friedrich, Adrián David Sierra, Jessica Mariel Regge, María Victoria Santilli, Maria Cecilia Trotta, Aldana Secchiari, Florencia Domaica, Carolina Ines Fuertes, Mercedes Beatriz Zwirner, Norberto Walter |
author |
Friedrich, Adrián David |
author_facet |
Friedrich, Adrián David Sierra, Jessica Mariel Regge, María Victoria Santilli, Maria Cecilia Trotta, Aldana Secchiari, Florencia Domaica, Carolina Ines Fuertes, Mercedes Beatriz Zwirner, Norberto Walter |
author_role |
author |
author2 |
Sierra, Jessica Mariel Regge, María Victoria Santilli, Maria Cecilia Trotta, Aldana Secchiari, Florencia Domaica, Carolina Ines Fuertes, Mercedes Beatriz Zwirner, Norberto Walter |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Curino, Alejandro Carlos Maccioni. Mariana Schaiquevich, Paula Duran, Hebe Alicia |
dc.subject.none.fl_str_mv |
ORGANOPHOSPHATE PESTICIDES NK CELLS CYTOTOXICITY |
topic |
ORGANOPHOSPHATE PESTICIDES NK CELLS CYTOTOXICITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Chlorpyrifos (CPF) and Glyphosate (GLY) are organophosphate pesticides widely used in agriculture. Several evidences suggest that CPF- and GLY-based pesticides are genotoxic and their use has been linked to the increased frequency of malignancies observed in highly fumigated rural areas. However, their effect on the immune system, including cells involved in immunosurveillance of tumor cells, has been poorly explored. Here, we investigated the effects of two commercial formulations containing CPF (Clorpi48) and GLY (Roundup Plus) as well as their isolated active principles on the phenotype and function of human NK cells. First, we identified sub-apoptotic doses of these substances by flow cytometry performing a dose-response curve with human peripheral blood mononuclear cells (PMBC). Next, using such sub-apoptotic doses, we analyzed the phenotype of pesticide-treated NK cells. We observed a pesticide dose-dependent reduction in the expression of CD16 and CD62L on NK cells after a 24 h of culture with CPF, GLY, Roundup and Clorpi48. Moreover, a diminished frequency of IFN--producing NK cells was observed upon exposure of cytokine-stimulated NK cells to 5 mM GLY, 0.05 mM Roundup, 0.01 mM Clorpi48 but not to 0.02 mM CPF (p<0.05). Moreover, NK cell-mediated cytotoxicity against K562 cells was also affected by pesticide treatment. After 0.05 mM Roundup and 0.01 mM Clorpi48 treatment, cytotoxicity was reduced by 27% (p<0.01) and 29% (p<0.001), respectively. In accordance with previous scientific evidence, final formulations of pesticides (which include additional compounds such as polyethoxylated alkylamines surfactants that facilitate their absorption by cells and tissues) showed a more potent effect on phenotype and function of NK cells than the isolated compounds. Therefore, we conclude that GLY- and CPF-based pesticides affect NK phenotype and function, which might impact on their ability to detect and eliminate nascent tumor cells. Fil: Friedrich, Adrián David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Bioquímica y Farmacia. Cátedra de Inmunología; Argentina Fil: Sierra, Jessica Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Regge, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Santilli, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Trotta, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Secchiari, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Domaica, Carolina Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Fuertes, Mercedes Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Zwirner, Norberto Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Annual Meeting of Bioscience Societies 2021: LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC), LXIX Annual Meeting of Sociedad Argentina de Inmunología(SAI), LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE), XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-Ar) Buenos Aires Argentina Sociedad Argentina de Investigación Clinica Sociedad Argentina de Inmunología Asociación Argentina de Farmacología Experimental Asociación Argentina de Nanomedicina |
description |
Chlorpyrifos (CPF) and Glyphosate (GLY) are organophosphate pesticides widely used in agriculture. Several evidences suggest that CPF- and GLY-based pesticides are genotoxic and their use has been linked to the increased frequency of malignancies observed in highly fumigated rural areas. However, their effect on the immune system, including cells involved in immunosurveillance of tumor cells, has been poorly explored. Here, we investigated the effects of two commercial formulations containing CPF (Clorpi48) and GLY (Roundup Plus) as well as their isolated active principles on the phenotype and function of human NK cells. First, we identified sub-apoptotic doses of these substances by flow cytometry performing a dose-response curve with human peripheral blood mononuclear cells (PMBC). Next, using such sub-apoptotic doses, we analyzed the phenotype of pesticide-treated NK cells. We observed a pesticide dose-dependent reduction in the expression of CD16 and CD62L on NK cells after a 24 h of culture with CPF, GLY, Roundup and Clorpi48. Moreover, a diminished frequency of IFN--producing NK cells was observed upon exposure of cytokine-stimulated NK cells to 5 mM GLY, 0.05 mM Roundup, 0.01 mM Clorpi48 but not to 0.02 mM CPF (p<0.05). Moreover, NK cell-mediated cytotoxicity against K562 cells was also affected by pesticide treatment. After 0.05 mM Roundup and 0.01 mM Clorpi48 treatment, cytotoxicity was reduced by 27% (p<0.01) and 29% (p<0.001), respectively. In accordance with previous scientific evidence, final formulations of pesticides (which include additional compounds such as polyethoxylated alkylamines surfactants that facilitate their absorption by cells and tissues) showed a more potent effect on phenotype and function of NK cells than the isolated compounds. Therefore, we conclude that GLY- and CPF-based pesticides affect NK phenotype and function, which might impact on their ability to detect and eliminate nascent tumor cells. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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http://hdl.handle.net/11336/152182 Phenotype and functional alterations of human NK cells by organophosphate pesticides; Annual Meeting of Bioscience Societies 2021: LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC), LXIX Annual Meeting of Sociedad Argentina de Inmunología(SAI), LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE), XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-Ar); Buenos Aires; Argentina; 2021; 138-138 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/152182 |
identifier_str_mv |
Phenotype and functional alterations of human NK cells by organophosphate pesticides; Annual Meeting of Bioscience Societies 2021: LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC), LXIX Annual Meeting of Sociedad Argentina de Inmunología(SAI), LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE), XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-Ar); Buenos Aires; Argentina; 2021; 138-138 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/ |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Fundación Revista Medicina |
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Fundación Revista Medicina |
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