The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium

Autores
Mateos, Melina Valeria; Kamerbeek, Constanza Belén; Giusto, Norma Maria; Salvador, Gabriela Alejandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The retinal pigment epithelium (RPE) plays an important immunological role in the retina and it is involved in many ocular inflammatory diseases that may end in loss of vision and blindness. In this work the role of phospholipase D (PLD) classical isoforms, PLD1 and PLD2, in the inflammatory response of human RPE cells (ARPE-19) was studied. ARPE-19 cells exposed to lipopolysaccharide (LPS, 10 µg/ml) displayed increased levels of NO production and diminished mitochondrial function after 48 h of incubation. Furthermore, 24 h LPS treatment strongly induced cyclooxygenase-2 (COX-2) expression and activation of extracellular signal-regulated kinase (ERK1/2). EGFP-PLDs showed the typical subcellular localization, perinuclear for PLD1 and plasma membrane for PLD2. LPS increased PLD activity by 90% with respect to the control. The presence of PLD1 inhibitor (EVJ 0.15 µM) or PLD2 inhibitor (APV 0.5 µM) reduced LPS-induced COX-2 induction but only PLD2 inhibition reduced ERK1/2 activation. Mitochondrial function was restored after inhibition of PLD2 and ERK1/2. These findings evidence the participation of PLD2 as a promoter of RPE inflammatory response through ERK1/2 and COX-2 regulation. Our results demonstrate for the first time distinctive roles of PLD isoforms in pathological conditions in RPE.
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Kamerbeek, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Materia
Arpe-19 Cells
Retinal Pigment Epithelium
Inflammation
Phospholipase D
Cyclooxygenase-2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4503
Acceder
id CONICETDig_dfbcba5df4718c0c73a267450e606640
oai_identifier_str oai:ri.conicet.gov.ar:11336/4503
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The phospholipase D pathway mediates the inflammatory response of the retinal pigment epitheliumMateos, Melina ValeriaKamerbeek, Constanza BelénGiusto, Norma MariaSalvador, Gabriela AlejandraArpe-19 CellsRetinal Pigment EpitheliumInflammationPhospholipase DCyclooxygenase-2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The retinal pigment epithelium (RPE) plays an important immunological role in the retina and it is involved in many ocular inflammatory diseases that may end in loss of vision and blindness. In this work the role of phospholipase D (PLD) classical isoforms, PLD1 and PLD2, in the inflammatory response of human RPE cells (ARPE-19) was studied. ARPE-19 cells exposed to lipopolysaccharide (LPS, 10 µg/ml) displayed increased levels of NO production and diminished mitochondrial function after 48 h of incubation. Furthermore, 24 h LPS treatment strongly induced cyclooxygenase-2 (COX-2) expression and activation of extracellular signal-regulated kinase (ERK1/2). EGFP-PLDs showed the typical subcellular localization, perinuclear for PLD1 and plasma membrane for PLD2. LPS increased PLD activity by 90% with respect to the control. The presence of PLD1 inhibitor (EVJ 0.15 µM) or PLD2 inhibitor (APV 0.5 µM) reduced LPS-induced COX-2 induction but only PLD2 inhibition reduced ERK1/2 activation. Mitochondrial function was restored after inhibition of PLD2 and ERK1/2. These findings evidence the participation of PLD2 as a promoter of RPE inflammatory response through ERK1/2 and COX-2 regulation. Our results demonstrate for the first time distinctive roles of PLD isoforms in pathological conditions in RPE.Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Kamerbeek, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaPergamon-elsevier Science Ltd2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4503Mateos, Melina Valeria; Kamerbeek, Constanza Belén; Giusto, Norma Maria; Salvador, Gabriela Alejandra; The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium; Pergamon-elsevier Science Ltd; International Journal Of Biochemistry And Cellular Biology; 55; 8-2014; 119-1281357-2725enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/25172550info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocel.2014.08.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:11:49Zoai:ri.conicet.gov.ar:11336/4503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:49.822CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
title The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
spellingShingle The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
Mateos, Melina Valeria
Arpe-19 Cells
Retinal Pigment Epithelium
Inflammation
Phospholipase D
Cyclooxygenase-2
title_short The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
title_full The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
title_fullStr The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
title_full_unstemmed The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
title_sort The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium
dc.creator.none.fl_str_mv Mateos, Melina Valeria
Kamerbeek, Constanza Belén
Giusto, Norma Maria
Salvador, Gabriela Alejandra
author Mateos, Melina Valeria
author_facet Mateos, Melina Valeria
Kamerbeek, Constanza Belén
Giusto, Norma Maria
Salvador, Gabriela Alejandra
author_role author
author2 Kamerbeek, Constanza Belén
Giusto, Norma Maria
Salvador, Gabriela Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Arpe-19 Cells
Retinal Pigment Epithelium
Inflammation
Phospholipase D
Cyclooxygenase-2
topic Arpe-19 Cells
Retinal Pigment Epithelium
Inflammation
Phospholipase D
Cyclooxygenase-2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The retinal pigment epithelium (RPE) plays an important immunological role in the retina and it is involved in many ocular inflammatory diseases that may end in loss of vision and blindness. In this work the role of phospholipase D (PLD) classical isoforms, PLD1 and PLD2, in the inflammatory response of human RPE cells (ARPE-19) was studied. ARPE-19 cells exposed to lipopolysaccharide (LPS, 10 µg/ml) displayed increased levels of NO production and diminished mitochondrial function after 48 h of incubation. Furthermore, 24 h LPS treatment strongly induced cyclooxygenase-2 (COX-2) expression and activation of extracellular signal-regulated kinase (ERK1/2). EGFP-PLDs showed the typical subcellular localization, perinuclear for PLD1 and plasma membrane for PLD2. LPS increased PLD activity by 90% with respect to the control. The presence of PLD1 inhibitor (EVJ 0.15 µM) or PLD2 inhibitor (APV 0.5 µM) reduced LPS-induced COX-2 induction but only PLD2 inhibition reduced ERK1/2 activation. Mitochondrial function was restored after inhibition of PLD2 and ERK1/2. These findings evidence the participation of PLD2 as a promoter of RPE inflammatory response through ERK1/2 and COX-2 regulation. Our results demonstrate for the first time distinctive roles of PLD isoforms in pathological conditions in RPE.
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Kamerbeek, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
description The retinal pigment epithelium (RPE) plays an important immunological role in the retina and it is involved in many ocular inflammatory diseases that may end in loss of vision and blindness. In this work the role of phospholipase D (PLD) classical isoforms, PLD1 and PLD2, in the inflammatory response of human RPE cells (ARPE-19) was studied. ARPE-19 cells exposed to lipopolysaccharide (LPS, 10 µg/ml) displayed increased levels of NO production and diminished mitochondrial function after 48 h of incubation. Furthermore, 24 h LPS treatment strongly induced cyclooxygenase-2 (COX-2) expression and activation of extracellular signal-regulated kinase (ERK1/2). EGFP-PLDs showed the typical subcellular localization, perinuclear for PLD1 and plasma membrane for PLD2. LPS increased PLD activity by 90% with respect to the control. The presence of PLD1 inhibitor (EVJ 0.15 µM) or PLD2 inhibitor (APV 0.5 µM) reduced LPS-induced COX-2 induction but only PLD2 inhibition reduced ERK1/2 activation. Mitochondrial function was restored after inhibition of PLD2 and ERK1/2. These findings evidence the participation of PLD2 as a promoter of RPE inflammatory response through ERK1/2 and COX-2 regulation. Our results demonstrate for the first time distinctive roles of PLD isoforms in pathological conditions in RPE.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4503
Mateos, Melina Valeria; Kamerbeek, Constanza Belén; Giusto, Norma Maria; Salvador, Gabriela Alejandra; The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium; Pergamon-elsevier Science Ltd; International Journal Of Biochemistry And Cellular Biology; 55; 8-2014; 119-128
1357-2725
url http://hdl.handle.net/11336/4503
identifier_str_mv Mateos, Melina Valeria; Kamerbeek, Constanza Belén; Giusto, Norma Maria; Salvador, Gabriela Alejandra; The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium; Pergamon-elsevier Science Ltd; International Journal Of Biochemistry And Cellular Biology; 55; 8-2014; 119-128
1357-2725
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/25172550
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocel.2014.08.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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