Collection, handling and care of cephalopod eggs

• Autores: Villanueva, Roger; Darmaillacq, Anne Sophie; Kuba, Michael J.; Roumbedakis, Katina; Nabhitabhata, Jaruwat; Almansa, Eduardo; Caamal Monsreal, Claudia; Court, Melanie; Dickel, Ludovic; Durante, Erica D.; Elagoz, Ali M.; Escolar, Oscar; Fernández Álvarez, Fernando Ángel; Galindo Sánchez, Clara E.; Ganias, Kostas; Gibbons, Connor J.; Goerger, Alice; Gouveneaux, Anaïd; Grasse, Bret; Guerra, Ángel; Jozet Alves, Christelle; Lacoue Labarthe, Thomas; Martínez Yebra, Lucía; Ortiz, Nicolás; Medeiros, Sylvia L. S.; Tomano, Satoshi; Trueblood, Lloyd A.; Vidal, Erica A. G.; Vilarnau, Diego G.; Voss, Gjendine
• Año de publicación: 2025
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: CEPHALOPODS EMBRYOLOGY; EGG HANDLING TECHNIQUES; EMBRYONIC DEVELOPMENT; ETHICS IN CEPHALOPODS RESEARCH
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Research on cephalopod early ontogeny has significantly advanced in recent decades, including embryo organogenesis and neurogenesis and early behavioural adaptations, particularly in commercially important and coastal species. Within this context, here we compiled current knowledge on the collection, handling and care under experimental conditions and monitoring in the field of cephalopod eggs and egg masses. It covers field observations and egg collection methods, as well as laboratory incubation protocols for eggs maintained with and without maternal care. It is emphasized how abiotic and biotic factors, including temperature, salinity, oxygen and maternal condition, shape embryonic development and hatchling survival and viability. Monitoring methods for cephalopod egg masses in the wild and the effects of natural threats such as storms and predators are reviewed. Anthropogenic impacts such as pollution, fisheries and climate change are also discussed. Technological advances have enabled finer analysis of neural development as the embryo grows, while ethical considerations regarding embryonic sentience capacity and welfare conditions are becoming central to current experimental designs. Based on current knowledge, it is recommended to apply ethical considerations and the 3Rs principle (Replacement, Reduction, Refinement) for cephalopod embryos following the onset of organogenesis. Thus, a list of potential indicators of health and welfare that could be used for assessing and monitoring cephalopod embryos and hatchlings is proposed. Overall, this synthesis aims to serve as a guide for advancing egg collection and laboratory incubation methodologies, as well as adopting ethical handling protocols to improve cephalopod embryo care and quality.
Fil: Villanueva, Roger. Instituto de Ciencias del Mar; España. Consejo Superior de Investigaciones Científicas; España
Fil: Darmaillacq, Anne Sophie. Universite de Caen Basse Normandie; Francia
Fil: Kuba, Michael J.. University of Naples; Italia
Fil: Roumbedakis, Katina. Instituto de Ciencias del Mar; España. Consejo Superior de Investigaciones Científicas; España
Fil: Nabhitabhata, Jaruwat. Universiti Sains Malaysia; Malasia
Fil: Almansa, Eduardo. Centro Oceanográfico de Canarias; España
Fil: Caamal Monsreal, Claudia. Universidad Nacional Autonoma de Mexico. Facultad de Ciencias. Unidad Multidisciplinaria de Docencia E Investigación.; México
Fil: Court, Melanie. Universidade Nova de Lisboa; Portugal
Fil: Dickel, Ludovic. Université de Caen Normandie; Francia
Fil: Durante, Erica D.. University of South Australia; Australia
Fil: Elagoz, Ali M.. Katholieke Universiteit Leuven; Bélgica
Fil: Escolar, Oscar. Instituto de Ciencias del Mar; España. Consejo Superior de Investigaciones Científicas; España
Fil: Fernández Álvarez, Fernando Ángel. Centro Oceanográfico de Gijón; España
Fil: Galindo Sánchez, Clara E.. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México
Fil: Ganias, Kostas. Aristotle University of Thessaloniki; Grecia
Fil: Gibbons, Connor J.. Columbia University; Estados Unidos
Fil: Goerger, Alice. Universite de Rennes I; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Gouveneaux, Anaïd. Université de Caen Normandie; Francia. Universite de Rennes I; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Grasse, Bret. University Of Chicago. Marine Biological Laboratory;
Fil: Guerra, Ángel. Instituto de Investigaciones Marinas; España. Consejo Superior de Investigaciones Científicas; España
Fil: Jozet Alves, Christelle. Université de Caen Normandie; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Lacoue Labarthe, Thomas. La Rochelle Université; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Martínez Yebra, Lucía. Sociedad Española de Malacología; España
Fil: Ortiz, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Medeiros, Sylvia L. S.. Universidad Tecnológica Nacional; Argentina
Fil: Tomano, Satoshi. University of Tokyo; Japón
Fil: Trueblood, Lloyd A.. La Sierra University; Estados Unidos
Fil: Vidal, Erica A. G.. University of Parana; Brasil
Fil: Vilarnau, Diego G.. Institut de Ciencies del Mar; España. Consejo Superior de Investigaciones Científicas; España
Fil: Voss, Gjendine. University Of Chicago. Marine Biological Laboratory;