The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos

Autores
Herkovits, Jorge; Perez Coll, Cristina Silvia; Stockert, Juan C.; Blázquez, Alfonso
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The photodynamic action is exerted by photogenerated reactive oxygen species (ROS) produced after cellular accumulation of a photosensitizer and irradiation with visible light. AMPHITOX test was employed successfully for standardized hazard assessment studies including phototoxic effects as well as the possibility to prevent/reduce adverse effects of different environmental agents. The main purpose of this study was to evaluate the toxicity and phototoxicity of 6 dyes and to establish the relationship between adverse effects and possible photodynamic activity. For this purpose the NOEC-48 h values were evaluated by means of  the AMPHITOX test conducted with Bufo arenarum embryos at the end of their development (stage 25) for the following dyes: acridine orange (AO), phloxin (Ph), phloxin B (PhB), rose bengal B (RB), toluidine blue O (TB), and pyronine Y (PY), dissolved in AMPHITOX solution (AS). In previous studies, it was determined that irradiation with white light at a dose of 43.2 J/cm2 (72 mW/cm2 for 10 min) is appropriate to explore the photodynamic action of chemicals. Thus, for the photodynamic experiments, embryos were treated with a range of concentrations bellow the NOEC values and after 48 h of exposure they were thoroughly washed and irradiated. The NOEC-48 h values (in mg/L) for chemical toxicity and NOEC-48 h values after light irradiation were: AO 1 and 0.5; PhB 1 and 0.7; RB 5 and 1; TB 10 and 5; Ph 10 and 10; PY 20 and 20, respectively. The survival of control embryos (maintained in AS and irradiated for 10 min) was not affected. By comparing the chemical/photochemical toxicity ratios of these substances it results that the order for photodynamic action could be expressed as RB > TB > AO > PhB, the remaining dyes showing no phototoxicity. These results point out the possibility to evaluate the photodynamic effect of chemicals by means of a simple toxicity test employing amphibian embryos.
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente; Argentina. Universidad Autónoma de Madrid; España
Fil: Blázquez, Alfonso. Universidad Autónoma de Madrid; España
Materia
photodynamic action
dyes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243328
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243328
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryosHerkovits, JorgePerez Coll, Cristina SilviaStockert, Juan C.Blázquez, Alfonsophotodynamic actiondyeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The photodynamic action is exerted by photogenerated reactive oxygen species (ROS) produced after cellular accumulation of a photosensitizer and irradiation with visible light. AMPHITOX test was employed successfully for standardized hazard assessment studies including phototoxic effects as well as the possibility to prevent/reduce adverse effects of different environmental agents. The main purpose of this study was to evaluate the toxicity and phototoxicity of 6 dyes and to establish the relationship between adverse effects and possible photodynamic activity. For this purpose the NOEC-48 h values were evaluated by means of  the AMPHITOX test conducted with Bufo arenarum embryos at the end of their development (stage 25) for the following dyes: acridine orange (AO), phloxin (Ph), phloxin B (PhB), rose bengal B (RB), toluidine blue O (TB), and pyronine Y (PY), dissolved in AMPHITOX solution (AS). In previous studies, it was determined that irradiation with white light at a dose of 43.2 J/cm2 (72 mW/cm2 for 10 min) is appropriate to explore the photodynamic action of chemicals. Thus, for the photodynamic experiments, embryos were treated with a range of concentrations bellow the NOEC values and after 48 h of exposure they were thoroughly washed and irradiated. The NOEC-48 h values (in mg/L) for chemical toxicity and NOEC-48 h values after light irradiation were: AO 1 and 0.5; PhB 1 and 0.7; RB 5 and 1; TB 10 and 5; Ph 10 and 10; PY 20 and 20, respectively. The survival of control embryos (maintained in AS and irradiated for 10 min) was not affected. By comparing the chemical/photochemical toxicity ratios of these substances it results that the order for photodynamic action could be expressed as RB > TB > AO > PhB, the remaining dyes showing no phototoxicity. These results point out the possibility to evaluate the photodynamic effect of chemicals by means of a simple toxicity test employing amphibian embryos.Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente; Argentina. Universidad Autónoma de Madrid; EspañaFil: Blázquez, Alfonso. Universidad Autónoma de Madrid; EspañaDr Jyoti Garg2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243328Herkovits, Jorge; Perez Coll, Cristina Silvia; Stockert, Juan C.; Blázquez, Alfonso; The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos; Dr Jyoti Garg; Research Journal Of Chemistry And Environment; 11; 12-2007; 86-910972-0626CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:13Zoai:ri.conicet.gov.ar:11336/243328instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:13.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
title The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
spellingShingle The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
Herkovits, Jorge
photodynamic action
dyes
title_short The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
title_full The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
title_fullStr The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
title_full_unstemmed The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
title_sort The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos
dc.creator.none.fl_str_mv Herkovits, Jorge
Perez Coll, Cristina Silvia
Stockert, Juan C.
Blázquez, Alfonso
author Herkovits, Jorge
author_facet Herkovits, Jorge
Perez Coll, Cristina Silvia
Stockert, Juan C.
Blázquez, Alfonso
author_role author
author2 Perez Coll, Cristina Silvia
Stockert, Juan C.
Blázquez, Alfonso
author2_role author
author
author
dc.subject.none.fl_str_mv photodynamic action
dyes
topic photodynamic action
dyes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The photodynamic action is exerted by photogenerated reactive oxygen species (ROS) produced after cellular accumulation of a photosensitizer and irradiation with visible light. AMPHITOX test was employed successfully for standardized hazard assessment studies including phototoxic effects as well as the possibility to prevent/reduce adverse effects of different environmental agents. The main purpose of this study was to evaluate the toxicity and phototoxicity of 6 dyes and to establish the relationship between adverse effects and possible photodynamic activity. For this purpose the NOEC-48 h values were evaluated by means of  the AMPHITOX test conducted with Bufo arenarum embryos at the end of their development (stage 25) for the following dyes: acridine orange (AO), phloxin (Ph), phloxin B (PhB), rose bengal B (RB), toluidine blue O (TB), and pyronine Y (PY), dissolved in AMPHITOX solution (AS). In previous studies, it was determined that irradiation with white light at a dose of 43.2 J/cm2 (72 mW/cm2 for 10 min) is appropriate to explore the photodynamic action of chemicals. Thus, for the photodynamic experiments, embryos were treated with a range of concentrations bellow the NOEC values and after 48 h of exposure they were thoroughly washed and irradiated. The NOEC-48 h values (in mg/L) for chemical toxicity and NOEC-48 h values after light irradiation were: AO 1 and 0.5; PhB 1 and 0.7; RB 5 and 1; TB 10 and 5; Ph 10 and 10; PY 20 and 20, respectively. The survival of control embryos (maintained in AS and irradiated for 10 min) was not affected. By comparing the chemical/photochemical toxicity ratios of these substances it results that the order for photodynamic action could be expressed as RB > TB > AO > PhB, the remaining dyes showing no phototoxicity. These results point out the possibility to evaluate the photodynamic effect of chemicals by means of a simple toxicity test employing amphibian embryos.
Fil: Herkovits, Jorge. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Coll, Cristina Silvia. Fundación Pro Salud y Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Stockert, Juan C.. Fundación Pro Salud y Medio Ambiente; Argentina. Universidad Autónoma de Madrid; España
Fil: Blázquez, Alfonso. Universidad Autónoma de Madrid; España
description The photodynamic action is exerted by photogenerated reactive oxygen species (ROS) produced after cellular accumulation of a photosensitizer and irradiation with visible light. AMPHITOX test was employed successfully for standardized hazard assessment studies including phototoxic effects as well as the possibility to prevent/reduce adverse effects of different environmental agents. The main purpose of this study was to evaluate the toxicity and phototoxicity of 6 dyes and to establish the relationship between adverse effects and possible photodynamic activity. For this purpose the NOEC-48 h values were evaluated by means of  the AMPHITOX test conducted with Bufo arenarum embryos at the end of their development (stage 25) for the following dyes: acridine orange (AO), phloxin (Ph), phloxin B (PhB), rose bengal B (RB), toluidine blue O (TB), and pyronine Y (PY), dissolved in AMPHITOX solution (AS). In previous studies, it was determined that irradiation with white light at a dose of 43.2 J/cm2 (72 mW/cm2 for 10 min) is appropriate to explore the photodynamic action of chemicals. Thus, for the photodynamic experiments, embryos were treated with a range of concentrations bellow the NOEC values and after 48 h of exposure they were thoroughly washed and irradiated. The NOEC-48 h values (in mg/L) for chemical toxicity and NOEC-48 h values after light irradiation were: AO 1 and 0.5; PhB 1 and 0.7; RB 5 and 1; TB 10 and 5; Ph 10 and 10; PY 20 and 20, respectively. The survival of control embryos (maintained in AS and irradiated for 10 min) was not affected. By comparing the chemical/photochemical toxicity ratios of these substances it results that the order for photodynamic action could be expressed as RB > TB > AO > PhB, the remaining dyes showing no phototoxicity. These results point out the possibility to evaluate the photodynamic effect of chemicals by means of a simple toxicity test employing amphibian embryos.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243328
Herkovits, Jorge; Perez Coll, Cristina Silvia; Stockert, Juan C.; Blázquez, Alfonso; The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos; Dr Jyoti Garg; Research Journal Of Chemistry And Environment; 11; 12-2007; 86-91
0972-0626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243328
identifier_str_mv Herkovits, Jorge; Perez Coll, Cristina Silvia; Stockert, Juan C.; Blázquez, Alfonso; The screening of photodynamic toxicity of dyes by means of a bioassay using amphibian embryos; Dr Jyoti Garg; Research Journal Of Chemistry And Environment; 11; 12-2007; 86-91
0972-0626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Dr Jyoti Garg
publisher.none.fl_str_mv Dr Jyoti Garg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083378922651648
score 13.22299

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