Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes

Autores
Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society.
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Alicante; España
Fil: Berna, Antonio. Universidad de Alicante; España
Fil: Feliu, Juan Miguel. Universidad de Alicante; España
Materia
Ir Spectroscopy
Bacterial Adhesion
Interfacial Chemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72537

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network_name_str CONICET Digital (CONICET)
spelling Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodesBusalmen, Juan PabloBerna, AntonioFeliu, Juan MiguelIr SpectroscopyBacterial AdhesionInterfacial Chemistryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society.Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Alicante; EspañaFil: Berna, Antonio. Universidad de Alicante; EspañaFil: Feliu, Juan Miguel. Universidad de Alicante; EspañaAmerican Chemical Society2007-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72537Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel; Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes; American Chemical Society; Langmuir; 23; 11; 5-2007; 6459-64660743-7463CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/la700406qinfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la700406qinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:38Zoai:ri.conicet.gov.ar:11336/72537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:38.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
title Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
spellingShingle Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
Busalmen, Juan Pablo
Ir Spectroscopy
Bacterial Adhesion
Interfacial Chemistry
title_short Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
title_full Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
title_fullStr Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
title_full_unstemmed Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
title_sort Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
dc.creator.none.fl_str_mv Busalmen, Juan Pablo
Berna, Antonio
Feliu, Juan Miguel
author Busalmen, Juan Pablo
author_facet Busalmen, Juan Pablo
Berna, Antonio
Feliu, Juan Miguel
author_role author
author2 Berna, Antonio
Feliu, Juan Miguel
author2_role author
author
dc.subject.none.fl_str_mv Ir Spectroscopy
Bacterial Adhesion
Interfacial Chemistry
topic Ir Spectroscopy
Bacterial Adhesion
Interfacial Chemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society.
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Alicante; España
Fil: Berna, Antonio. Universidad de Alicante; España
Fil: Feliu, Juan Miguel. Universidad de Alicante; España
description The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society.
publishDate 2007
dc.date.none.fl_str_mv 2007-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72537
Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel; Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes; American Chemical Society; Langmuir; 23; 11; 5-2007; 6459-6466
0743-7463
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72537
identifier_str_mv Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel; Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes; American Chemical Society; Langmuir; 23; 11; 5-2007; 6459-6466
0743-7463
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/la700406q
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la700406q
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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