Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes
- Autores
- Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society.
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Alicante; España
Fil: Berna, Antonio. Universidad de Alicante; España
Fil: Feliu, Juan Miguel. Universidad de Alicante; España - Materia
-
Ir Spectroscopy
Bacterial Adhesion
Interfacial Chemistry - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/72537
Ver los metadatos del registro completo
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Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodesBusalmen, Juan PabloBerna, AntonioFeliu, Juan MiguelIr SpectroscopyBacterial AdhesionInterfacial Chemistryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society.Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Alicante; EspañaFil: Berna, Antonio. Universidad de Alicante; EspañaFil: Feliu, Juan Miguel. Universidad de Alicante; EspañaAmerican Chemical Society2007-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72537Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel; Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes; American Chemical Society; Langmuir; 23; 11; 5-2007; 6459-64660743-7463CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/la700406qinfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la700406qinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:38Zoai:ri.conicet.gov.ar:11336/72537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:38.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
title |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
spellingShingle |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes Busalmen, Juan Pablo Ir Spectroscopy Bacterial Adhesion Interfacial Chemistry |
title_short |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
title_full |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
title_fullStr |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
title_full_unstemmed |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
title_sort |
Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes |
dc.creator.none.fl_str_mv |
Busalmen, Juan Pablo Berna, Antonio Feliu, Juan Miguel |
author |
Busalmen, Juan Pablo |
author_facet |
Busalmen, Juan Pablo Berna, Antonio Feliu, Juan Miguel |
author_role |
author |
author2 |
Berna, Antonio Feliu, Juan Miguel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Ir Spectroscopy Bacterial Adhesion Interfacial Chemistry |
topic |
Ir Spectroscopy Bacterial Adhesion Interfacial Chemistry |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society. Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Alicante; España Fil: Berna, Antonio. Universidad de Alicante; España Fil: Feliu, Juan Miguel. Universidad de Alicante; España |
description |
The interaction between bacterial cells of Pseudomonas fluorescens (ATCC 17552) and gold electrodes was analyzed by cyclic voltammetry (CV) and attenuated total reflection-surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). The voltammetric evaluation of cell adsorption showed a decrease in the double-layer capacitance of polyoriented single-crystal gold electrodes with cell adhesion. As followed by IR spectroscopy in the ATR configuration, the adsorption of bacterial cells onto thin-film gold electrodes was mainly indicated by the increase in intensity with time of amide I and amide II protein-related bands at 1664 and 1549 cm-1, respectively. Bands at 1448 and 2900 cm-1 corresponding to the scissoring and the stretching bands of CH2 were also detected, together with a minor peak at 1407 cm-1 due to the vs COO- stretching. Weak signals at 1237 cm-1 were due to amide III, and a broad band between 1100 and 1200cm-1 indicated the presence of alcohol groups. Bacteria were found to displace water molecules and anions coadsorbed on the surface in order to interact with the electrode intimately. This fact was evidenced in the SEIRAS spectra by the negative features appearing at 3450 and 3575 cm -1, corresponding to interfacial water directly interacting with the electrode and water associated with chloride ions adsorbed on the electrode, respectively. Experiments in deuterated water confirmed these assignments and allowed a better estimation of amide absorption bands. In CV experiments, an oxidation process was observed at potentials higher than 0.4 V that was dependent on the exposure time of electrodes in concentrated bacterial suspensions. Adsorbed bacterial cells were found to get closer to the gold surface during oxidation, as indicated by the concomitant increment in the main IR bacterial signals including amideI, a sharp band at 1240 cm-1, and a broad one at 1120 cm-1 related to phosphate groups in the bacterial membranes. It is proposed to be due to the oxidation of lipopolysaccharides on the outermost bacterial surface. © 2007 American Chemical Society. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/72537 Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel; Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes; American Chemical Society; Langmuir; 23; 11; 5-2007; 6459-6466 0743-7463 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/72537 |
identifier_str_mv |
Busalmen, Juan Pablo; Berna, Antonio; Feliu, Juan Miguel; Spectroelectrochemical examination of the interaction between bacterial cells and gold electrodes; American Chemical Society; Langmuir; 23; 11; 5-2007; 6459-6466 0743-7463 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1021/la700406q info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/la700406q |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614169256525824 |
score |
13.070432 |