Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect

Autores
Giorgi, Exequiel David; Genovés, Sofía; Díaz, María Eugenia; Municoy, Sofia; Desimone, Martín Federico; de Marzi, Mauricio Cesar
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Silica NPs (SiNPs) used as a platform to deliver molecules have huge potential for biomedical applications. In order to generate new immunomodulatory tools, 2 variants of SiNPs were synthesized and 3 proteins were adsorbed over their surface: bovine serum albumin and the cytokines IL-1β and TGF-β. Protein adsorption was analyzed according to Langmuir and Freundlich models. The adsorption isotherm of IL-1β on both SiNP variants had a good fit to the Freundlich model, indicating the formation of a protein multilayer around the NPs. For BSA and TGF-β isotherms, the fit to the Langmuir model was better, evidencing the presence of a protein monolayer on the NPs. SiNPs@TGF-β complexes were tested in THP-1 cells (human monocytes cells). The complexes reduced cellular metabolic activity and did not cause an increase in nitric oxide expression, which is related to the immunosuppressive activity of TGF-β, but was potentiated and prolonged over time compared to the cytokine alone. These nanocomplexes could be important tools for use as nanoinmunomodulators (NIMs) for the therapeutic treatment of inflammatory diseases.
Fil: Giorgi, Exequiel David. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Genovés, Sofía. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Díaz, María Eugenia. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Municoy, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: de Marzi, Mauricio Cesar. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Materia
immunomodulatory proteins
silica nanoparticles
nanoimmunomodulators
adsorption
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229052
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/229052
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effectGiorgi, Exequiel DavidGenovés, SofíaDíaz, María EugeniaMunicoy, SofiaDesimone, Martín Federicode Marzi, Mauricio Cesarimmunomodulatory proteinssilica nanoparticlesnanoimmunomodulatorsadsorptionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Silica NPs (SiNPs) used as a platform to deliver molecules have huge potential for biomedical applications. In order to generate new immunomodulatory tools, 2 variants of SiNPs were synthesized and 3 proteins were adsorbed over their surface: bovine serum albumin and the cytokines IL-1β and TGF-β. Protein adsorption was analyzed according to Langmuir and Freundlich models. The adsorption isotherm of IL-1β on both SiNP variants had a good fit to the Freundlich model, indicating the formation of a protein multilayer around the NPs. For BSA and TGF-β isotherms, the fit to the Langmuir model was better, evidencing the presence of a protein monolayer on the NPs. SiNPs@TGF-β complexes were tested in THP-1 cells (human monocytes cells). The complexes reduced cellular metabolic activity and did not cause an increase in nitric oxide expression, which is related to the immunosuppressive activity of TGF-β, but was potentiated and prolonged over time compared to the cytokine alone. These nanocomplexes could be important tools for use as nanoinmunomodulators (NIMs) for the therapeutic treatment of inflammatory diseases.Fil: Giorgi, Exequiel David. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Genovés, Sofía. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Díaz, María Eugenia. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Municoy, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: de Marzi, Mauricio Cesar. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaRoyal Society of Chemistry2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229052Giorgi, Exequiel David; Genovés, Sofía; Díaz, María Eugenia; Municoy, Sofia; Desimone, Martín Federico; et al.; Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect; Royal Society of Chemistry; Materials Advances; 5; 2; 12-2023; 777-7872633-5409CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/D3MA00776Finfo:eu-repo/semantics/altIdentifier/doi/10.1039/d3ma00776finfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:33Zoai:ri.conicet.gov.ar:11336/229052instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:33.479CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
title Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
spellingShingle Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
Giorgi, Exequiel David
immunomodulatory proteins
silica nanoparticles
nanoimmunomodulators
adsorption
title_short Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
title_full Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
title_fullStr Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
title_full_unstemmed Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
title_sort Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect
dc.creator.none.fl_str_mv Giorgi, Exequiel David
Genovés, Sofía
Díaz, María Eugenia
Municoy, Sofia
Desimone, Martín Federico
de Marzi, Mauricio Cesar
author Giorgi, Exequiel David
author_facet Giorgi, Exequiel David
Genovés, Sofía
Díaz, María Eugenia
Municoy, Sofia
Desimone, Martín Federico
de Marzi, Mauricio Cesar
author_role author
author2 Genovés, Sofía
Díaz, María Eugenia
Municoy, Sofia
Desimone, Martín Federico
de Marzi, Mauricio Cesar
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv immunomodulatory proteins
silica nanoparticles
nanoimmunomodulators
adsorption
topic immunomodulatory proteins
silica nanoparticles
nanoimmunomodulators
adsorption
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Silica NPs (SiNPs) used as a platform to deliver molecules have huge potential for biomedical applications. In order to generate new immunomodulatory tools, 2 variants of SiNPs were synthesized and 3 proteins were adsorbed over their surface: bovine serum albumin and the cytokines IL-1β and TGF-β. Protein adsorption was analyzed according to Langmuir and Freundlich models. The adsorption isotherm of IL-1β on both SiNP variants had a good fit to the Freundlich model, indicating the formation of a protein multilayer around the NPs. For BSA and TGF-β isotherms, the fit to the Langmuir model was better, evidencing the presence of a protein monolayer on the NPs. SiNPs@TGF-β complexes were tested in THP-1 cells (human monocytes cells). The complexes reduced cellular metabolic activity and did not cause an increase in nitric oxide expression, which is related to the immunosuppressive activity of TGF-β, but was potentiated and prolonged over time compared to the cytokine alone. These nanocomplexes could be important tools for use as nanoinmunomodulators (NIMs) for the therapeutic treatment of inflammatory diseases.
Fil: Giorgi, Exequiel David. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Genovés, Sofía. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Díaz, María Eugenia. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Municoy, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Desimone, Martín Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: de Marzi, Mauricio Cesar. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
description Silica NPs (SiNPs) used as a platform to deliver molecules have huge potential for biomedical applications. In order to generate new immunomodulatory tools, 2 variants of SiNPs were synthesized and 3 proteins were adsorbed over their surface: bovine serum albumin and the cytokines IL-1β and TGF-β. Protein adsorption was analyzed according to Langmuir and Freundlich models. The adsorption isotherm of IL-1β on both SiNP variants had a good fit to the Freundlich model, indicating the formation of a protein multilayer around the NPs. For BSA and TGF-β isotherms, the fit to the Langmuir model was better, evidencing the presence of a protein monolayer on the NPs. SiNPs@TGF-β complexes were tested in THP-1 cells (human monocytes cells). The complexes reduced cellular metabolic activity and did not cause an increase in nitric oxide expression, which is related to the immunosuppressive activity of TGF-β, but was potentiated and prolonged over time compared to the cytokine alone. These nanocomplexes could be important tools for use as nanoinmunomodulators (NIMs) for the therapeutic treatment of inflammatory diseases.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229052
Giorgi, Exequiel David; Genovés, Sofía; Díaz, María Eugenia; Municoy, Sofia; Desimone, Martín Federico; et al.; Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect; Royal Society of Chemistry; Materials Advances; 5; 2; 12-2023; 777-787
2633-5409
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229052
identifier_str_mv Giorgi, Exequiel David; Genovés, Sofía; Díaz, María Eugenia; Municoy, Sofia; Desimone, Martín Federico; et al.; Adsorption of immunomodulatory proteins over silica nanoparticles and the in vitro effect; Royal Society of Chemistry; Materials Advances; 5; 2; 12-2023; 777-787
2633-5409
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/D3MA00776F
info:eu-repo/semantics/altIdentifier/doi/10.1039/d3ma00776f
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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