Role of GPER in the anterior pituitary gland focusing on lactotroph function

Autores
Camilletti, María Andrea; Abeledo Machado, Alejandra Inés; Ferraris, Maria Jimena; Pérez, Pablo Aníbal; Faraoni, Erika Yanil; Pisera, Daniel Alberto; Gutiérrez, Silvina; Diaz, Graciela Susana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ovarian steroids control a variety of physiological functions. They exert actions throughclassical nuclear steroid receptors, but rapid non-genomic actions through specificmembrane steroid receptors have been also described. In this study, we demonstratethat the G-protein-coupled estrogen receptor (GPER) is expressed in the rat pituitarygland and, at a high level, in the lactotroph population. Our results revealed that~40% of the anterior pituitary cells are GPER positive and ~35% of the lactotrophs areGPER positive. By immunocytochemical and immuno-electron-microscopy studies, wedemonstrated that GPER is localized in the plasmatic membrane but is also associatedto the endoplasmic reticulum in rat lactotrophs. Moreover, we found that local Gperexpression is regulated negatively by 17β-estradiol (E2) and progesterone (P4) andfluctuates during the estrus cycle, being minimal in proestrus. Interestingly, lack ofovarian steroids after an ovariectomy (OVX) significantly increased pituitary GPERexpression specifically in the three morphologically different subtypes of lactotrophs.We found a rapid estradiol stimulatory effect on PRL secretion mediated by GPER,both in vitro and ex vivo, using a GPER agonist G1, and this effect was prevented by theGPER antagonist G36, demonstrating a novel role for this receptor. Then, the increasedpituitary GPER expression after OVX could lead to alterations in the pituitary functionas all three lactotroph subtypes are target of GPER ligand and could be involved in thePRL secretion mediated by GPER. Therefore, it should be taken into consideration in theresponse of the gland to an eventual hormone replacement therapy
Fil: Camilletti, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Abeledo Machado, Alejandra Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Ferraris, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Pérez, Pablo Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Faraoni, Erika Yanil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pisera, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Gutiérrez, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
PITUITARY
LACTOTROPHS
GPER
PROLACTIN
OVARIECTOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103452
Acceder
id CONICETDig_de2e482c04c84c87e36d537e0aa3064a
oai_identifier_str oai:ri.conicet.gov.ar:11336/103452
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of GPER in the anterior pituitary gland focusing on lactotroph functionCamilletti, María AndreaAbeledo Machado, Alejandra InésFerraris, Maria JimenaPérez, Pablo AníbalFaraoni, Erika YanilPisera, Daniel AlbertoGutiérrez, SilvinaDiaz, Graciela SusanaPITUITARYLACTOTROPHSGPERPROLACTINOVARIECTOMYhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Ovarian steroids control a variety of physiological functions. They exert actions throughclassical nuclear steroid receptors, but rapid non-genomic actions through specificmembrane steroid receptors have been also described. In this study, we demonstratethat the G-protein-coupled estrogen receptor (GPER) is expressed in the rat pituitarygland and, at a high level, in the lactotroph population. Our results revealed that~40% of the anterior pituitary cells are GPER positive and ~35% of the lactotrophs areGPER positive. By immunocytochemical and immuno-electron-microscopy studies, wedemonstrated that GPER is localized in the plasmatic membrane but is also associatedto the endoplasmic reticulum in rat lactotrophs. Moreover, we found that local Gperexpression is regulated negatively by 17β-estradiol (E2) and progesterone (P4) andfluctuates during the estrus cycle, being minimal in proestrus. Interestingly, lack ofovarian steroids after an ovariectomy (OVX) significantly increased pituitary GPERexpression specifically in the three morphologically different subtypes of lactotrophs.We found a rapid estradiol stimulatory effect on PRL secretion mediated by GPER,both in vitro and ex vivo, using a GPER agonist G1, and this effect was prevented by theGPER antagonist G36, demonstrating a novel role for this receptor. Then, the increasedpituitary GPER expression after OVX could lead to alterations in the pituitary functionas all three lactotroph subtypes are target of GPER ligand and could be involved in thePRL secretion mediated by GPER. Therefore, it should be taken into consideration in theresponse of the gland to an eventual hormone replacement therapyFil: Camilletti, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Abeledo Machado, Alejandra Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Ferraris, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Pérez, Pablo Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Faraoni, Erika Yanil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pisera, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Gutiérrez, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaBioScientifica2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103452Camilletti, María Andrea; Abeledo Machado, Alejandra Inés; Ferraris, Maria Jimena; Pérez, Pablo Aníbal; Faraoni, Erika Yanil; et al.; Role of GPER in the anterior pituitary gland focusing on lactotroph function; BioScientifica; Journal of Endocrinology; 240; 2; 10-2018; 99-1100022-0795CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://joe.bioscientifica.com/view/journals/joe/240/2/JOE-18-0402.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-18-0402info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:10Zoai:ri.conicet.gov.ar:11336/103452instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:11.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of GPER in the anterior pituitary gland focusing on lactotroph function
title Role of GPER in the anterior pituitary gland focusing on lactotroph function
spellingShingle Role of GPER in the anterior pituitary gland focusing on lactotroph function
Camilletti, María Andrea
PITUITARY
LACTOTROPHS
GPER
PROLACTIN
OVARIECTOMY
title_short Role of GPER in the anterior pituitary gland focusing on lactotroph function
title_full Role of GPER in the anterior pituitary gland focusing on lactotroph function
title_fullStr Role of GPER in the anterior pituitary gland focusing on lactotroph function
title_full_unstemmed Role of GPER in the anterior pituitary gland focusing on lactotroph function
title_sort Role of GPER in the anterior pituitary gland focusing on lactotroph function
dc.creator.none.fl_str_mv Camilletti, María Andrea
Abeledo Machado, Alejandra Inés
Ferraris, Maria Jimena
Pérez, Pablo Aníbal
Faraoni, Erika Yanil
Pisera, Daniel Alberto
Gutiérrez, Silvina
Diaz, Graciela Susana
author Camilletti, María Andrea
author_facet Camilletti, María Andrea
Abeledo Machado, Alejandra Inés
Ferraris, Maria Jimena
Pérez, Pablo Aníbal
Faraoni, Erika Yanil
Pisera, Daniel Alberto
Gutiérrez, Silvina
Diaz, Graciela Susana
author_role author
author2 Abeledo Machado, Alejandra Inés
Ferraris, Maria Jimena
Pérez, Pablo Aníbal
Faraoni, Erika Yanil
Pisera, Daniel Alberto
Gutiérrez, Silvina
Diaz, Graciela Susana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PITUITARY
LACTOTROPHS
GPER
PROLACTIN
OVARIECTOMY
topic PITUITARY
LACTOTROPHS
GPER
PROLACTIN
OVARIECTOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ovarian steroids control a variety of physiological functions. They exert actions throughclassical nuclear steroid receptors, but rapid non-genomic actions through specificmembrane steroid receptors have been also described. In this study, we demonstratethat the G-protein-coupled estrogen receptor (GPER) is expressed in the rat pituitarygland and, at a high level, in the lactotroph population. Our results revealed that~40% of the anterior pituitary cells are GPER positive and ~35% of the lactotrophs areGPER positive. By immunocytochemical and immuno-electron-microscopy studies, wedemonstrated that GPER is localized in the plasmatic membrane but is also associatedto the endoplasmic reticulum in rat lactotrophs. Moreover, we found that local Gperexpression is regulated negatively by 17β-estradiol (E2) and progesterone (P4) andfluctuates during the estrus cycle, being minimal in proestrus. Interestingly, lack ofovarian steroids after an ovariectomy (OVX) significantly increased pituitary GPERexpression specifically in the three morphologically different subtypes of lactotrophs.We found a rapid estradiol stimulatory effect on PRL secretion mediated by GPER,both in vitro and ex vivo, using a GPER agonist G1, and this effect was prevented by theGPER antagonist G36, demonstrating a novel role for this receptor. Then, the increasedpituitary GPER expression after OVX could lead to alterations in the pituitary functionas all three lactotroph subtypes are target of GPER ligand and could be involved in thePRL secretion mediated by GPER. Therefore, it should be taken into consideration in theresponse of the gland to an eventual hormone replacement therapy
Fil: Camilletti, María Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Abeledo Machado, Alejandra Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Ferraris, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Pérez, Pablo Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Faraoni, Erika Yanil. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pisera, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Gutiérrez, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Diaz, Graciela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Ovarian steroids control a variety of physiological functions. They exert actions throughclassical nuclear steroid receptors, but rapid non-genomic actions through specificmembrane steroid receptors have been also described. In this study, we demonstratethat the G-protein-coupled estrogen receptor (GPER) is expressed in the rat pituitarygland and, at a high level, in the lactotroph population. Our results revealed that~40% of the anterior pituitary cells are GPER positive and ~35% of the lactotrophs areGPER positive. By immunocytochemical and immuno-electron-microscopy studies, wedemonstrated that GPER is localized in the plasmatic membrane but is also associatedto the endoplasmic reticulum in rat lactotrophs. Moreover, we found that local Gperexpression is regulated negatively by 17β-estradiol (E2) and progesterone (P4) andfluctuates during the estrus cycle, being minimal in proestrus. Interestingly, lack ofovarian steroids after an ovariectomy (OVX) significantly increased pituitary GPERexpression specifically in the three morphologically different subtypes of lactotrophs.We found a rapid estradiol stimulatory effect on PRL secretion mediated by GPER,both in vitro and ex vivo, using a GPER agonist G1, and this effect was prevented by theGPER antagonist G36, demonstrating a novel role for this receptor. Then, the increasedpituitary GPER expression after OVX could lead to alterations in the pituitary functionas all three lactotroph subtypes are target of GPER ligand and could be involved in thePRL secretion mediated by GPER. Therefore, it should be taken into consideration in theresponse of the gland to an eventual hormone replacement therapy
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103452
Camilletti, María Andrea; Abeledo Machado, Alejandra Inés; Ferraris, Maria Jimena; Pérez, Pablo Aníbal; Faraoni, Erika Yanil; et al.; Role of GPER in the anterior pituitary gland focusing on lactotroph function; BioScientifica; Journal of Endocrinology; 240; 2; 10-2018; 99-110
0022-0795
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103452
identifier_str_mv Camilletti, María Andrea; Abeledo Machado, Alejandra Inés; Ferraris, Maria Jimena; Pérez, Pablo Aníbal; Faraoni, Erika Yanil; et al.; Role of GPER in the anterior pituitary gland focusing on lactotroph function; BioScientifica; Journal of Endocrinology; 240; 2; 10-2018; 99-110
0022-0795
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://joe.bioscientifica.com/view/journals/joe/240/2/JOE-18-0402.xml
info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-18-0402
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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