The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification

Autores
Daverio, Maria Silvana; Di Rocco, Florencia; Vidal Rioja, Lidia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Growth hormone (GH) is a peptide hormone found in most vertebrates, which has important effects on the muscular development during growth as well as on the production and composition of milk during lactation. Here, we report the sequence and organization of the GH gene, its promoter region and several single nucleotide polymorphisms (SNPs) in the llama (Lama glama), a domestic camelid from South America. Like in other mammals, the GH gene in the llama is organized in five exons separated by four introns. Comparison of its coding sequence with that of other cetartiodactyl species showed identities that ranged from 90.3% when compared with that of sheep to 99.1% when compared with that of camel (Camelus dromedarius). Introns were less conserved, particularly Intron C, which in the llama was found to be considerably shorter than in other species. In the promoter region, the TATA box showed a T/C substitution in the first base, also present in the camel. Other regulatory elements of the promoter region are fully conserved, except Sp1, CRE, and the proximal Pit-1 element, which present substitutions with respect to other cetartiodactyl species. Sequencing of the GH gene in a sample of 10 llamas allowed us to identify 15 SNPs, mainly located in non-coding regions. However, three of them were found in the promoter and the 5'-UTR of Exon 1, regions involved in transcription and translation processes. Moreover, in other species, intronic polymorphisms were found to be associated with growth and carcass traits. Therefore, further studies in llama herds will be necessary to infer the role of the polymorphisms found in this paper. © 2011 Elsevier B.V.
Fil: Daverio, Maria Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Di Rocco, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Vidal Rioja, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Materia
Growth Hormone
Llama
Nucleotide Sequence
Polymorphisms
Promoter
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67865
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67865
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identificationDaverio, Maria SilvanaDi Rocco, FlorenciaVidal Rioja, LidiaGrowth HormoneLlamaNucleotide SequencePolymorphismsPromoterhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Growth hormone (GH) is a peptide hormone found in most vertebrates, which has important effects on the muscular development during growth as well as on the production and composition of milk during lactation. Here, we report the sequence and organization of the GH gene, its promoter region and several single nucleotide polymorphisms (SNPs) in the llama (Lama glama), a domestic camelid from South America. Like in other mammals, the GH gene in the llama is organized in five exons separated by four introns. Comparison of its coding sequence with that of other cetartiodactyl species showed identities that ranged from 90.3% when compared with that of sheep to 99.1% when compared with that of camel (Camelus dromedarius). Introns were less conserved, particularly Intron C, which in the llama was found to be considerably shorter than in other species. In the promoter region, the TATA box showed a T/C substitution in the first base, also present in the camel. Other regulatory elements of the promoter region are fully conserved, except Sp1, CRE, and the proximal Pit-1 element, which present substitutions with respect to other cetartiodactyl species. Sequencing of the GH gene in a sample of 10 llamas allowed us to identify 15 SNPs, mainly located in non-coding regions. However, three of them were found in the promoter and the 5'-UTR of Exon 1, regions involved in transcription and translation processes. Moreover, in other species, intronic polymorphisms were found to be associated with growth and carcass traits. Therefore, further studies in llama herds will be necessary to infer the role of the polymorphisms found in this paper. © 2011 Elsevier B.V.Fil: Daverio, Maria Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Di Rocco, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Vidal Rioja, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaElsevier Science2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67865Daverio, Maria Silvana; Di Rocco, Florencia; Vidal Rioja, Lidia; The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification; Elsevier Science; Journal of Small Ruminant Research; 103; 2-3; 4-2012; 108-1110921-4488CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2011.08.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448811003038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:04Zoai:ri.conicet.gov.ar:11336/67865instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:05.085CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
title The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
spellingShingle The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
Daverio, Maria Silvana
Growth Hormone
Llama
Nucleotide Sequence
Polymorphisms
Promoter
title_short The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
title_full The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
title_fullStr The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
title_full_unstemmed The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
title_sort The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification
dc.creator.none.fl_str_mv Daverio, Maria Silvana
Di Rocco, Florencia
Vidal Rioja, Lidia
author Daverio, Maria Silvana
author_facet Daverio, Maria Silvana
Di Rocco, Florencia
Vidal Rioja, Lidia
author_role author
author2 Di Rocco, Florencia
Vidal Rioja, Lidia
author2_role author
author
dc.subject.none.fl_str_mv Growth Hormone
Llama
Nucleotide Sequence
Polymorphisms
Promoter
topic Growth Hormone
Llama
Nucleotide Sequence
Polymorphisms
Promoter
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Growth hormone (GH) is a peptide hormone found in most vertebrates, which has important effects on the muscular development during growth as well as on the production and composition of milk during lactation. Here, we report the sequence and organization of the GH gene, its promoter region and several single nucleotide polymorphisms (SNPs) in the llama (Lama glama), a domestic camelid from South America. Like in other mammals, the GH gene in the llama is organized in five exons separated by four introns. Comparison of its coding sequence with that of other cetartiodactyl species showed identities that ranged from 90.3% when compared with that of sheep to 99.1% when compared with that of camel (Camelus dromedarius). Introns were less conserved, particularly Intron C, which in the llama was found to be considerably shorter than in other species. In the promoter region, the TATA box showed a T/C substitution in the first base, also present in the camel. Other regulatory elements of the promoter region are fully conserved, except Sp1, CRE, and the proximal Pit-1 element, which present substitutions with respect to other cetartiodactyl species. Sequencing of the GH gene in a sample of 10 llamas allowed us to identify 15 SNPs, mainly located in non-coding regions. However, three of them were found in the promoter and the 5'-UTR of Exon 1, regions involved in transcription and translation processes. Moreover, in other species, intronic polymorphisms were found to be associated with growth and carcass traits. Therefore, further studies in llama herds will be necessary to infer the role of the polymorphisms found in this paper. © 2011 Elsevier B.V.
Fil: Daverio, Maria Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Di Rocco, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Vidal Rioja, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
description Growth hormone (GH) is a peptide hormone found in most vertebrates, which has important effects on the muscular development during growth as well as on the production and composition of milk during lactation. Here, we report the sequence and organization of the GH gene, its promoter region and several single nucleotide polymorphisms (SNPs) in the llama (Lama glama), a domestic camelid from South America. Like in other mammals, the GH gene in the llama is organized in five exons separated by four introns. Comparison of its coding sequence with that of other cetartiodactyl species showed identities that ranged from 90.3% when compared with that of sheep to 99.1% when compared with that of camel (Camelus dromedarius). Introns were less conserved, particularly Intron C, which in the llama was found to be considerably shorter than in other species. In the promoter region, the TATA box showed a T/C substitution in the first base, also present in the camel. Other regulatory elements of the promoter region are fully conserved, except Sp1, CRE, and the proximal Pit-1 element, which present substitutions with respect to other cetartiodactyl species. Sequencing of the GH gene in a sample of 10 llamas allowed us to identify 15 SNPs, mainly located in non-coding regions. However, three of them were found in the promoter and the 5'-UTR of Exon 1, regions involved in transcription and translation processes. Moreover, in other species, intronic polymorphisms were found to be associated with growth and carcass traits. Therefore, further studies in llama herds will be necessary to infer the role of the polymorphisms found in this paper. © 2011 Elsevier B.V.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67865
Daverio, Maria Silvana; Di Rocco, Florencia; Vidal Rioja, Lidia; The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification; Elsevier Science; Journal of Small Ruminant Research; 103; 2-3; 4-2012; 108-111
0921-4488
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67865
identifier_str_mv Daverio, Maria Silvana; Di Rocco, Florencia; Vidal Rioja, Lidia; The llama (Lama glama) growth hormone gene: Sequence, organization and SNP identification; Elsevier Science; Journal of Small Ruminant Research; 103; 2-3; 4-2012; 108-111
0921-4488
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2011.08.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448811003038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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