Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats

Autores
Ortega, Hugo Hector; Salvetti, Natalia Raquel; Baravalle, Celina; Lorente, J. A.; Mira, G. A.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by estradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by estradiol. Adult male rats were injected daily with either 50 µg of estradiol or estradiol plus testosterone propionate (25mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by RIA. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against Chromogranin A, S-100 Protein, P Substance, Synaptofisin, Neurofilament, Gliofibrillary Acidic Protein and Neuron Specific Enolase were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In estradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled. Keywords: rat – Leydig cell – neuroendocrin markers– estrogens
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lorente, J. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Mira, G. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
neuroendocrine markers
Leydig cells
rats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106558
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/106558
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult RatsOrtega, Hugo HectorSalvetti, Natalia RaquelBaravalle, CelinaLorente, J. A.Mira, G. A.neuroendocrine markersLeydig cellsratshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by estradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by estradiol. Adult male rats were injected daily with either 50 µg of estradiol or estradiol plus testosterone propionate (25mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by RIA. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against Chromogranin A, S-100 Protein, P Substance, Synaptofisin, Neurofilament, Gliofibrillary Acidic Protein and Neuron Specific Enolase were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In estradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled. Keywords: rat – Leydig cell – neuroendocrin markers– estrogensFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Lorente, J. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Mira, G. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaWiley Blackwell Publishing, Inc2006-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106558Ortega, Hugo Hector; Salvetti, Natalia Raquel; Baravalle, Celina; Lorente, J. A.; Mira, G. A.; Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 41; 3; 6-2006; 204-2090936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2006.00669.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:23Zoai:ri.conicet.gov.ar:11336/106558instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:23.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
title Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
spellingShingle Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
Ortega, Hugo Hector
neuroendocrine markers
Leydig cells
rats
title_short Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
title_full Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
title_fullStr Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
title_full_unstemmed Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
title_sort Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats
dc.creator.none.fl_str_mv Ortega, Hugo Hector
Salvetti, Natalia Raquel
Baravalle, Celina
Lorente, J. A.
Mira, G. A.
author Ortega, Hugo Hector
author_facet Ortega, Hugo Hector
Salvetti, Natalia Raquel
Baravalle, Celina
Lorente, J. A.
Mira, G. A.
author_role author
author2 Salvetti, Natalia Raquel
Baravalle, Celina
Lorente, J. A.
Mira, G. A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv neuroendocrine markers
Leydig cells
rats
topic neuroendocrine markers
Leydig cells
rats
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by estradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by estradiol. Adult male rats were injected daily with either 50 µg of estradiol or estradiol plus testosterone propionate (25mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by RIA. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against Chromogranin A, S-100 Protein, P Substance, Synaptofisin, Neurofilament, Gliofibrillary Acidic Protein and Neuron Specific Enolase were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In estradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled. Keywords: rat – Leydig cell – neuroendocrin markers– estrogens
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lorente, J. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Mira, G. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description The objectives of this work were to determine the changes in the expression of neuroendocrine markers in Leydig cell by estradiol treatment, and to determine whether testosterone is able to recover partially the effects of hormonal suppression induced by estradiol. Adult male rats were injected daily with either 50 µg of estradiol or estradiol plus testosterone propionate (25mg every 3 days) for 15 days. The animals were sacrificed and testicles were dissected and processed by routine histological protocols. FSH and LH serum levels were determined by RIA. The visualization of antigens was achieved by the streptavidin-peroxidase immunohistochemical method. Antibodies against Chromogranin A, S-100 Protein, P Substance, Synaptofisin, Neurofilament, Gliofibrillary Acidic Protein and Neuron Specific Enolase were used. The mean LH and FSH serum concentrations were consistently suppressed with hormonal treatments. Intermediate filaments (NF and GFAP) showed no difference in their expression. The expression of S-100, NSE and SYN was significantly lower in both hormone-treated groups. In estradiol-treated rats, the immunoreactivity of CrA and SP decreased significantly but was restored after testosterone supplementation. Although the nature and functions of many of these substances in Leydig cells remain unknown, these results are consistent with the hypothesis that the expression of some neuroendocrine markers is hormonally controlled. Keywords: rat – Leydig cell – neuroendocrin markers– estrogens
publishDate 2006
dc.date.none.fl_str_mv 2006-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106558
Ortega, Hugo Hector; Salvetti, Natalia Raquel; Baravalle, Celina; Lorente, J. A.; Mira, G. A.; Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 41; 3; 6-2006; 204-209
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106558
identifier_str_mv Ortega, Hugo Hector; Salvetti, Natalia Raquel; Baravalle, Celina; Lorente, J. A.; Mira, G. A.; Oestradiol Induced Inhibition of Neuroendocrine Marker Expression in Leydig Cells of Adult Rats; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 41; 3; 6-2006; 204-209
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2006.00669.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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