Guanine crystals discovered in bacteria

Autores
Pavan, María Elisa; Movilla, Federico; Pavan, Esteban E.; Di Salvo, Florencia; López, Nancy Beatriz; Pettinari, María Julia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Guanine crystals are organic biogenic crystals found in many organisms. Due to their exceptionally high refractive index, they contribute to structural color and are responsible for the reflective effect in the skin and visual organs in animals such as fish, reptiles and spiders. Occurrence of these crystals in animals has been known for many years, and they have also been observed in eukaryotic microorganisms, but not in prokaryotes. In this work we report the discovery of extracellular crystals in bacteria, and reveal that they are composed of guanine, and particularly the unusual monohydrate form. We demonstrate the occurrence of these crystals in Aeromonas and other bacteria, and investigate the metabolic traits related to their synthesis. In all cases studied the presence of the guanine crystals in bacteria correlate with the absence of guanine deaminase, which could lead to guanine accumulation providing the substrate for crystal formation. Our finding of the hitherto unknown guanine crystal occurrence in prokaryotes extends the range of guanine crystal producing organisms to a new domain of life. Bacteria constitute a new and more accessible model to study the process of guanine crystal formation and assembly. This discovery opens countless chemical and biological questions, including those about the functional and adaptive significance of their production in these microorganisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications.Significance Guanine crystal formation is well known in animals such as fish, reptiles and arthropods (among other eukaryotic organisms), but its occurrence has never been reported in prokaryotes. This manuscript describes the discovery of extracellular guanine crystals in bacteria, and reveals that they are composed of the unusual monohydrate form of guanine. Knowledge of guanine crystal biosynthesis in bacteria could lead to a better understanding of their synthesis in other organisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications. Our finding extends the range of guanine crystal producing organisms to a new domain of life.
Fil: Pavan, María Elisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Movilla, Federico. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Pavan, Esteban E.. Politecnico di Milano; Italia
Fil: Di Salvo, Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: López, Nancy Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pettinari, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
GUANINE CRYSTALS
BACTERIUM
MELANIN
BIOGENIC CRYSTALS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215825
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215825
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Guanine crystals discovered in bacteriaPavan, María ElisaMovilla, FedericoPavan, Esteban E.Di Salvo, FlorenciaLópez, Nancy BeatrizPettinari, María JuliaGUANINE CRYSTALSBACTERIUMMELANINBIOGENIC CRYSTALShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Guanine crystals are organic biogenic crystals found in many organisms. Due to their exceptionally high refractive index, they contribute to structural color and are responsible for the reflective effect in the skin and visual organs in animals such as fish, reptiles and spiders. Occurrence of these crystals in animals has been known for many years, and they have also been observed in eukaryotic microorganisms, but not in prokaryotes. In this work we report the discovery of extracellular crystals in bacteria, and reveal that they are composed of guanine, and particularly the unusual monohydrate form. We demonstrate the occurrence of these crystals in Aeromonas and other bacteria, and investigate the metabolic traits related to their synthesis. In all cases studied the presence of the guanine crystals in bacteria correlate with the absence of guanine deaminase, which could lead to guanine accumulation providing the substrate for crystal formation. Our finding of the hitherto unknown guanine crystal occurrence in prokaryotes extends the range of guanine crystal producing organisms to a new domain of life. Bacteria constitute a new and more accessible model to study the process of guanine crystal formation and assembly. This discovery opens countless chemical and biological questions, including those about the functional and adaptive significance of their production in these microorganisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications.Significance Guanine crystal formation is well known in animals such as fish, reptiles and arthropods (among other eukaryotic organisms), but its occurrence has never been reported in prokaryotes. This manuscript describes the discovery of extracellular guanine crystals in bacteria, and reveals that they are composed of the unusual monohydrate form of guanine. Knowledge of guanine crystal biosynthesis in bacteria could lead to a better understanding of their synthesis in other organisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications. Our finding extends the range of guanine crystal producing organisms to a new domain of life.Fil: Pavan, María Elisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Movilla, Federico. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Pavan, Esteban E.. Politecnico di Milano; ItaliaFil: Di Salvo, Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: López, Nancy Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Pettinari, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaCold Spring Harbor Laboratory Press2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215825Pavan, María Elisa; Movilla, Federico; Pavan, Esteban E.; Di Salvo, Florencia; López, Nancy Beatriz; et al.; Guanine crystals discovered in bacteria; Cold Spring Harbor Laboratory Press; bioRxiv; 12-2022; 1-172692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1101/2022.12.02.518751info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2022.12.02.518751v2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:16Zoai:ri.conicet.gov.ar:11336/215825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:17.252CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Guanine crystals discovered in bacteria
title Guanine crystals discovered in bacteria
spellingShingle Guanine crystals discovered in bacteria
Pavan, María Elisa
GUANINE CRYSTALS
BACTERIUM
MELANIN
BIOGENIC CRYSTALS
title_short Guanine crystals discovered in bacteria
title_full Guanine crystals discovered in bacteria
title_fullStr Guanine crystals discovered in bacteria
title_full_unstemmed Guanine crystals discovered in bacteria
title_sort Guanine crystals discovered in bacteria
dc.creator.none.fl_str_mv Pavan, María Elisa
Movilla, Federico
Pavan, Esteban E.
Di Salvo, Florencia
López, Nancy Beatriz
Pettinari, María Julia
author Pavan, María Elisa
author_facet Pavan, María Elisa
Movilla, Federico
Pavan, Esteban E.
Di Salvo, Florencia
López, Nancy Beatriz
Pettinari, María Julia
author_role author
author2 Movilla, Federico
Pavan, Esteban E.
Di Salvo, Florencia
López, Nancy Beatriz
Pettinari, María Julia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GUANINE CRYSTALS
BACTERIUM
MELANIN
BIOGENIC CRYSTALS
topic GUANINE CRYSTALS
BACTERIUM
MELANIN
BIOGENIC CRYSTALS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Guanine crystals are organic biogenic crystals found in many organisms. Due to their exceptionally high refractive index, they contribute to structural color and are responsible for the reflective effect in the skin and visual organs in animals such as fish, reptiles and spiders. Occurrence of these crystals in animals has been known for many years, and they have also been observed in eukaryotic microorganisms, but not in prokaryotes. In this work we report the discovery of extracellular crystals in bacteria, and reveal that they are composed of guanine, and particularly the unusual monohydrate form. We demonstrate the occurrence of these crystals in Aeromonas and other bacteria, and investigate the metabolic traits related to their synthesis. In all cases studied the presence of the guanine crystals in bacteria correlate with the absence of guanine deaminase, which could lead to guanine accumulation providing the substrate for crystal formation. Our finding of the hitherto unknown guanine crystal occurrence in prokaryotes extends the range of guanine crystal producing organisms to a new domain of life. Bacteria constitute a new and more accessible model to study the process of guanine crystal formation and assembly. This discovery opens countless chemical and biological questions, including those about the functional and adaptive significance of their production in these microorganisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications.Significance Guanine crystal formation is well known in animals such as fish, reptiles and arthropods (among other eukaryotic organisms), but its occurrence has never been reported in prokaryotes. This manuscript describes the discovery of extracellular guanine crystals in bacteria, and reveals that they are composed of the unusual monohydrate form of guanine. Knowledge of guanine crystal biosynthesis in bacteria could lead to a better understanding of their synthesis in other organisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications. Our finding extends the range of guanine crystal producing organisms to a new domain of life.
Fil: Pavan, María Elisa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Movilla, Federico. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Pavan, Esteban E.. Politecnico di Milano; Italia
Fil: Di Salvo, Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: López, Nancy Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pettinari, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description Guanine crystals are organic biogenic crystals found in many organisms. Due to their exceptionally high refractive index, they contribute to structural color and are responsible for the reflective effect in the skin and visual organs in animals such as fish, reptiles and spiders. Occurrence of these crystals in animals has been known for many years, and they have also been observed in eukaryotic microorganisms, but not in prokaryotes. In this work we report the discovery of extracellular crystals in bacteria, and reveal that they are composed of guanine, and particularly the unusual monohydrate form. We demonstrate the occurrence of these crystals in Aeromonas and other bacteria, and investigate the metabolic traits related to their synthesis. In all cases studied the presence of the guanine crystals in bacteria correlate with the absence of guanine deaminase, which could lead to guanine accumulation providing the substrate for crystal formation. Our finding of the hitherto unknown guanine crystal occurrence in prokaryotes extends the range of guanine crystal producing organisms to a new domain of life. Bacteria constitute a new and more accessible model to study the process of guanine crystal formation and assembly. This discovery opens countless chemical and biological questions, including those about the functional and adaptive significance of their production in these microorganisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications.Significance Guanine crystal formation is well known in animals such as fish, reptiles and arthropods (among other eukaryotic organisms), but its occurrence has never been reported in prokaryotes. This manuscript describes the discovery of extracellular guanine crystals in bacteria, and reveals that they are composed of the unusual monohydrate form of guanine. Knowledge of guanine crystal biosynthesis in bacteria could lead to a better understanding of their synthesis in other organisms. It also paves the road for the development of simple and convenient processes to obtain biogenic guanine crystals for diverse applications. Our finding extends the range of guanine crystal producing organisms to a new domain of life.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215825
Pavan, María Elisa; Movilla, Federico; Pavan, Esteban E.; Di Salvo, Florencia; López, Nancy Beatriz; et al.; Guanine crystals discovered in bacteria; Cold Spring Harbor Laboratory Press; bioRxiv; 12-2022; 1-17
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215825
identifier_str_mv Pavan, María Elisa; Movilla, Federico; Pavan, Esteban E.; Di Salvo, Florencia; López, Nancy Beatriz; et al.; Guanine crystals discovered in bacteria; Cold Spring Harbor Laboratory Press; bioRxiv; 12-2022; 1-17
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1101/2022.12.02.518751
info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2022.12.02.518751v2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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