Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants

Autores
Mansilla, Andrea Yamila; Salcedo, Maria Florencia; Colman, Silvana Lorena; Chevalier, Merari T.; Lanfranconi, Matias R.; Alvarez, Vera Alejandra; Casalongue, Claudia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Salicylic acid (SA) is a phytohormone with a central role in plant protection against various types of stresses. Exogenous application of SA in plants often requires a fine adjustment of the pH in the applied solution as well as frequent foliar sprays with a rigorous handling of doses. This work investigates the preparation and characterization of nanocomposites composed of bentonite clays (Bent) and SA as bio-active to produce an efficient and stable hormonal formulation to be used in agriculture. Bent-SA was obtained via cationic exchange reactions of natural Bent with an aqueous solution of SA. The physicochemical properties of Bent-SA were studied in terms of Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). SA was intercalated in a 3.65% w/w interacting by electrostatic forces between Bent layers. The SA protective activity was investigated in Pseudomonas syringae pv. tomato DC3000 (Pto)-infected tomato plants (Solanum lycopersicum). The expression levels of pathogenesis-related proteins (PRs) were measured by real time quantitative RT-PCR. The genes PR1 and PR5 were up-regulated 2.2- and 1.23-fold, respectively, in 24 h-treated Bent-SA plants. Furthermore, PR3 protein level increased locally and systemically by Bent-SA treatment in plant tissues. The plant disease incidence was significantly reduced in Bent-SA pretreated seedlings. Analyses of DR5:GUS transgenic reporter tomato lines showed that Bent-SA effectiveness was preserved after vehiculization. It was concluded that Bent-SA can provide an environmentally friendly strategy to control tomato plants against Pto bacterial infection.
Fil: Mansilla, Andrea Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Salcedo, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Colman, Silvana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Chevalier, Merari T.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Lanfranconi, Matias R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
BENTONITE
PSEUDOMONAS SYRINGAE PV. TOMATO DC3000
SALICYLIC ACID
SOLANUM LYCOPERSICUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140295
Acceder
id CONICETDig_dcac006c46ef327fccdda3f42af0c2da
oai_identifier_str oai:ri.conicet.gov.ar:11336/140295
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plantsMansilla, Andrea YamilaSalcedo, Maria FlorenciaColman, Silvana LorenaChevalier, Merari T.Lanfranconi, Matias R.Alvarez, Vera AlejandraCasalongue, ClaudiaBENTONITEPSEUDOMONAS SYRINGAE PV. TOMATO DC3000SALICYLIC ACIDSOLANUM LYCOPERSICUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Salicylic acid (SA) is a phytohormone with a central role in plant protection against various types of stresses. Exogenous application of SA in plants often requires a fine adjustment of the pH in the applied solution as well as frequent foliar sprays with a rigorous handling of doses. This work investigates the preparation and characterization of nanocomposites composed of bentonite clays (Bent) and SA as bio-active to produce an efficient and stable hormonal formulation to be used in agriculture. Bent-SA was obtained via cationic exchange reactions of natural Bent with an aqueous solution of SA. The physicochemical properties of Bent-SA were studied in terms of Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). SA was intercalated in a 3.65% w/w interacting by electrostatic forces between Bent layers. The SA protective activity was investigated in Pseudomonas syringae pv. tomato DC3000 (Pto)-infected tomato plants (Solanum lycopersicum). The expression levels of pathogenesis-related proteins (PRs) were measured by real time quantitative RT-PCR. The genes PR1 and PR5 were up-regulated 2.2- and 1.23-fold, respectively, in 24 h-treated Bent-SA plants. Furthermore, PR3 protein level increased locally and systemically by Bent-SA treatment in plant tissues. The plant disease incidence was significantly reduced in Bent-SA pretreated seedlings. Analyses of DR5:GUS transgenic reporter tomato lines showed that Bent-SA effectiveness was preserved after vehiculization. It was concluded that Bent-SA can provide an environmentally friendly strategy to control tomato plants against Pto bacterial infection.Fil: Mansilla, Andrea Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Salcedo, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Colman, Silvana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Chevalier, Merari T.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Lanfranconi, Matias R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaSpringer2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140295Mansilla, Andrea Yamila; Salcedo, Maria Florencia; Colman, Silvana Lorena; Chevalier, Merari T.; Lanfranconi, Matias R.; et al.; Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants; Springer; European Journal of Plant Pathology; 158; 1; 7-2020; 211-2220929-1873CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10658-020-02067-winfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-020-02067-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:51Zoai:ri.conicet.gov.ar:11336/140295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:51.494CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
title Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
spellingShingle Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
Mansilla, Andrea Yamila
BENTONITE
PSEUDOMONAS SYRINGAE PV. TOMATO DC3000
SALICYLIC ACID
SOLANUM LYCOPERSICUM
title_short Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
title_full Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
title_fullStr Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
title_full_unstemmed Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
title_sort Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants
dc.creator.none.fl_str_mv Mansilla, Andrea Yamila
Salcedo, Maria Florencia
Colman, Silvana Lorena
Chevalier, Merari T.
Lanfranconi, Matias R.
Alvarez, Vera Alejandra
Casalongue, Claudia
author Mansilla, Andrea Yamila
author_facet Mansilla, Andrea Yamila
Salcedo, Maria Florencia
Colman, Silvana Lorena
Chevalier, Merari T.
Lanfranconi, Matias R.
Alvarez, Vera Alejandra
Casalongue, Claudia
author_role author
author2 Salcedo, Maria Florencia
Colman, Silvana Lorena
Chevalier, Merari T.
Lanfranconi, Matias R.
Alvarez, Vera Alejandra
Casalongue, Claudia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BENTONITE
PSEUDOMONAS SYRINGAE PV. TOMATO DC3000
SALICYLIC ACID
SOLANUM LYCOPERSICUM
topic BENTONITE
PSEUDOMONAS SYRINGAE PV. TOMATO DC3000
SALICYLIC ACID
SOLANUM LYCOPERSICUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Salicylic acid (SA) is a phytohormone with a central role in plant protection against various types of stresses. Exogenous application of SA in plants often requires a fine adjustment of the pH in the applied solution as well as frequent foliar sprays with a rigorous handling of doses. This work investigates the preparation and characterization of nanocomposites composed of bentonite clays (Bent) and SA as bio-active to produce an efficient and stable hormonal formulation to be used in agriculture. Bent-SA was obtained via cationic exchange reactions of natural Bent with an aqueous solution of SA. The physicochemical properties of Bent-SA were studied in terms of Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). SA was intercalated in a 3.65% w/w interacting by electrostatic forces between Bent layers. The SA protective activity was investigated in Pseudomonas syringae pv. tomato DC3000 (Pto)-infected tomato plants (Solanum lycopersicum). The expression levels of pathogenesis-related proteins (PRs) were measured by real time quantitative RT-PCR. The genes PR1 and PR5 were up-regulated 2.2- and 1.23-fold, respectively, in 24 h-treated Bent-SA plants. Furthermore, PR3 protein level increased locally and systemically by Bent-SA treatment in plant tissues. The plant disease incidence was significantly reduced in Bent-SA pretreated seedlings. Analyses of DR5:GUS transgenic reporter tomato lines showed that Bent-SA effectiveness was preserved after vehiculization. It was concluded that Bent-SA can provide an environmentally friendly strategy to control tomato plants against Pto bacterial infection.
Fil: Mansilla, Andrea Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Salcedo, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Colman, Silvana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Chevalier, Merari T.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Lanfranconi, Matias R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Alvarez, Vera Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Salicylic acid (SA) is a phytohormone with a central role in plant protection against various types of stresses. Exogenous application of SA in plants often requires a fine adjustment of the pH in the applied solution as well as frequent foliar sprays with a rigorous handling of doses. This work investigates the preparation and characterization of nanocomposites composed of bentonite clays (Bent) and SA as bio-active to produce an efficient and stable hormonal formulation to be used in agriculture. Bent-SA was obtained via cationic exchange reactions of natural Bent with an aqueous solution of SA. The physicochemical properties of Bent-SA were studied in terms of Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD) and Thermogravimetric Analysis (TGA). SA was intercalated in a 3.65% w/w interacting by electrostatic forces between Bent layers. The SA protective activity was investigated in Pseudomonas syringae pv. tomato DC3000 (Pto)-infected tomato plants (Solanum lycopersicum). The expression levels of pathogenesis-related proteins (PRs) were measured by real time quantitative RT-PCR. The genes PR1 and PR5 were up-regulated 2.2- and 1.23-fold, respectively, in 24 h-treated Bent-SA plants. Furthermore, PR3 protein level increased locally and systemically by Bent-SA treatment in plant tissues. The plant disease incidence was significantly reduced in Bent-SA pretreated seedlings. Analyses of DR5:GUS transgenic reporter tomato lines showed that Bent-SA effectiveness was preserved after vehiculization. It was concluded that Bent-SA can provide an environmentally friendly strategy to control tomato plants against Pto bacterial infection.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140295
Mansilla, Andrea Yamila; Salcedo, Maria Florencia; Colman, Silvana Lorena; Chevalier, Merari T.; Lanfranconi, Matias R.; et al.; Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants; Springer; European Journal of Plant Pathology; 158; 1; 7-2020; 211-222
0929-1873
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140295
identifier_str_mv Mansilla, Andrea Yamila; Salcedo, Maria Florencia; Colman, Silvana Lorena; Chevalier, Merari T.; Lanfranconi, Matias R.; et al.; Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants; Springer; European Journal of Plant Pathology; 158; 1; 7-2020; 211-222
0929-1873
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10658-020-02067-w
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10658-020-02067-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614412296519680
score 13.070432

Publicaciones similares