Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection

Autores
Balint Kurti, Peter; Simmons, Susan J.; Blum, James E.; Ballare, Carlos Luis; Stapleton, Ann E.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant leaves host a specific set of microbial epiphytes. Plant genetic and solar UV-B radiation effects on the diversity of the phyllosphere were examined by measuring epiphytic bacterial ribosomal DNA diversity in a maize recombinant inbred (RI) mapping population. Several chromosomal quantitative trait loci (QTL) with significant effects on bacterial diversity were identified, some of which had effects only in the presence of UV-B radiation and others that had effects both with and without UV-B. Candidate genes with allele-specific effects were mapped to the bacterial diversity chromosomal regions. A glutamate decarboxylase candidate gene was located at a UV-B-specific chromosomal locus, and in a comparison between two RI lines with contrasting bacterial diversity phenotypes, high bacterial diversity was associated with high levels of glutamate decarboxylase enzyme activity, a component of the gamma-aminobutyric acid (GABA) pathway. The bacterial diversity loci exhibited a significant overlap with loci connected with Southern leaf blight (SLB) susceptibility in the field. A SLB-resistant inbred genotype had less beta bacterial diversity, and antibiotic treatment of inbreds increased this diversity. These results suggest that the GABA pathway is genetically associated with phyllosphere bacterial diversity. Furthermore, the colocalization of QTL between low bacterial diversity and fungal blight-resistance and the increase in beta diversity in antibiotic-treated leaves suggest that occupation of leaf habitats by a particular set of suppressive bacteria may restrict phyllosphere bacterial variability and increase resistance to fungal infection. © 2010 The American Phytopathological Society.
Fil: Balint Kurti, Peter. North Carolina State University; Estados Unidos
Fil: Simmons, Susan J.. University of North Carolina at Wilmington; Estados Unidos
Fil: Blum, James E.. University of North Carolina at Wilmington; Estados Unidos
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Stapleton, Ann E.. University of North Carolina at Wilmington; Estados Unidos
Materia
QTL
UV
BACTERIA
DIVERSITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72308

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spelling Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infectionBalint Kurti, PeterSimmons, Susan J.Blum, James E.Ballare, Carlos LuisStapleton, Ann E.QTLUVBACTERIADIVERSITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant leaves host a specific set of microbial epiphytes. Plant genetic and solar UV-B radiation effects on the diversity of the phyllosphere were examined by measuring epiphytic bacterial ribosomal DNA diversity in a maize recombinant inbred (RI) mapping population. Several chromosomal quantitative trait loci (QTL) with significant effects on bacterial diversity were identified, some of which had effects only in the presence of UV-B radiation and others that had effects both with and without UV-B. Candidate genes with allele-specific effects were mapped to the bacterial diversity chromosomal regions. A glutamate decarboxylase candidate gene was located at a UV-B-specific chromosomal locus, and in a comparison between two RI lines with contrasting bacterial diversity phenotypes, high bacterial diversity was associated with high levels of glutamate decarboxylase enzyme activity, a component of the gamma-aminobutyric acid (GABA) pathway. The bacterial diversity loci exhibited a significant overlap with loci connected with Southern leaf blight (SLB) susceptibility in the field. A SLB-resistant inbred genotype had less beta bacterial diversity, and antibiotic treatment of inbreds increased this diversity. These results suggest that the GABA pathway is genetically associated with phyllosphere bacterial diversity. Furthermore, the colocalization of QTL between low bacterial diversity and fungal blight-resistance and the increase in beta diversity in antibiotic-treated leaves suggest that occupation of leaf habitats by a particular set of suppressive bacteria may restrict phyllosphere bacterial variability and increase resistance to fungal infection. © 2010 The American Phytopathological Society.Fil: Balint Kurti, Peter. North Carolina State University; Estados UnidosFil: Simmons, Susan J.. University of North Carolina at Wilmington; Estados UnidosFil: Blum, James E.. University of North Carolina at Wilmington; Estados UnidosFil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Stapleton, Ann E.. University of North Carolina at Wilmington; Estados UnidosAmerican Phytopathological Society2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72308Balint Kurti, Peter; Simmons, Susan J.; Blum, James E.; Ballare, Carlos Luis; Stapleton, Ann E.; Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 23; 4; 4-2010; 473-4840894-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-23-4-0473info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-4-0473info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:44Zoai:ri.conicet.gov.ar:11336/72308instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:45.015CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
title Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
spellingShingle Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
Balint Kurti, Peter
QTL
UV
BACTERIA
DIVERSITY
title_short Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
title_full Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
title_fullStr Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
title_full_unstemmed Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
title_sort Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection
dc.creator.none.fl_str_mv Balint Kurti, Peter
Simmons, Susan J.
Blum, James E.
Ballare, Carlos Luis
Stapleton, Ann E.
author Balint Kurti, Peter
author_facet Balint Kurti, Peter
Simmons, Susan J.
Blum, James E.
Ballare, Carlos Luis
Stapleton, Ann E.
author_role author
author2 Simmons, Susan J.
Blum, James E.
Ballare, Carlos Luis
Stapleton, Ann E.
author2_role author
author
author
author
dc.subject.none.fl_str_mv QTL
UV
BACTERIA
DIVERSITY
topic QTL
UV
BACTERIA
DIVERSITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant leaves host a specific set of microbial epiphytes. Plant genetic and solar UV-B radiation effects on the diversity of the phyllosphere were examined by measuring epiphytic bacterial ribosomal DNA diversity in a maize recombinant inbred (RI) mapping population. Several chromosomal quantitative trait loci (QTL) with significant effects on bacterial diversity were identified, some of which had effects only in the presence of UV-B radiation and others that had effects both with and without UV-B. Candidate genes with allele-specific effects were mapped to the bacterial diversity chromosomal regions. A glutamate decarboxylase candidate gene was located at a UV-B-specific chromosomal locus, and in a comparison between two RI lines with contrasting bacterial diversity phenotypes, high bacterial diversity was associated with high levels of glutamate decarboxylase enzyme activity, a component of the gamma-aminobutyric acid (GABA) pathway. The bacterial diversity loci exhibited a significant overlap with loci connected with Southern leaf blight (SLB) susceptibility in the field. A SLB-resistant inbred genotype had less beta bacterial diversity, and antibiotic treatment of inbreds increased this diversity. These results suggest that the GABA pathway is genetically associated with phyllosphere bacterial diversity. Furthermore, the colocalization of QTL between low bacterial diversity and fungal blight-resistance and the increase in beta diversity in antibiotic-treated leaves suggest that occupation of leaf habitats by a particular set of suppressive bacteria may restrict phyllosphere bacterial variability and increase resistance to fungal infection. © 2010 The American Phytopathological Society.
Fil: Balint Kurti, Peter. North Carolina State University; Estados Unidos
Fil: Simmons, Susan J.. University of North Carolina at Wilmington; Estados Unidos
Fil: Blum, James E.. University of North Carolina at Wilmington; Estados Unidos
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Stapleton, Ann E.. University of North Carolina at Wilmington; Estados Unidos
description Plant leaves host a specific set of microbial epiphytes. Plant genetic and solar UV-B radiation effects on the diversity of the phyllosphere were examined by measuring epiphytic bacterial ribosomal DNA diversity in a maize recombinant inbred (RI) mapping population. Several chromosomal quantitative trait loci (QTL) with significant effects on bacterial diversity were identified, some of which had effects only in the presence of UV-B radiation and others that had effects both with and without UV-B. Candidate genes with allele-specific effects were mapped to the bacterial diversity chromosomal regions. A glutamate decarboxylase candidate gene was located at a UV-B-specific chromosomal locus, and in a comparison between two RI lines with contrasting bacterial diversity phenotypes, high bacterial diversity was associated with high levels of glutamate decarboxylase enzyme activity, a component of the gamma-aminobutyric acid (GABA) pathway. The bacterial diversity loci exhibited a significant overlap with loci connected with Southern leaf blight (SLB) susceptibility in the field. A SLB-resistant inbred genotype had less beta bacterial diversity, and antibiotic treatment of inbreds increased this diversity. These results suggest that the GABA pathway is genetically associated with phyllosphere bacterial diversity. Furthermore, the colocalization of QTL between low bacterial diversity and fungal blight-resistance and the increase in beta diversity in antibiotic-treated leaves suggest that occupation of leaf habitats by a particular set of suppressive bacteria may restrict phyllosphere bacterial variability and increase resistance to fungal infection. © 2010 The American Phytopathological Society.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72308
Balint Kurti, Peter; Simmons, Susan J.; Blum, James E.; Ballare, Carlos Luis; Stapleton, Ann E.; Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 23; 4; 4-2010; 473-484
0894-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72308
identifier_str_mv Balint Kurti, Peter; Simmons, Susan J.; Blum, James E.; Ballare, Carlos Luis; Stapleton, Ann E.; Maize leaf epiphytic bacteria diversity patterns are genetically correlated with resistance to fungal pathogen infection; American Phytopathological Society; Molecular Plant-Microbe Interactions; 23; 4; 4-2010; 473-484
0894-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-23-4-0473
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-4-0473
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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