Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption

Autores
Fochesato, Analía Silvia; Cristofolini, Andrea Lorena; Poloni, Valeria Lorena; Magnoli, Alejandra Paola; Merkis, Cecilia Inés; Dogi, Cecilia Ana; Cavaglieri, Lilia Reneé
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective was to study the influence of culture medium and gastrointestinal environment on Saccharomyces cerevisiae RC016 cell wall polysaccharides profile and the aflatoxin B1 (AFB1) adsorption. Probiotic Saccharomyces cerevisiae RC016, isolated from the pig small intestine, has previously shown efficient mycotoxins adsorption in vitro including AFB1. In addition, it was able to survive under the gastrointestinal (GI) tract conditions and did not cause in vitro and in vivo genotoxicity or cytotoxicity. In this work, transmission electron microscopy (TEM) was used to show ultrastructural variations in cell morphology and, infrared spectroscopy (IR-frequency range 4000–500 cm−1) was used to show variations in the spectra of yeast cells cultured in different culture media, Yeast extract Peptone Dextrose (YPD) medium and Dried distillers grains (DDG) medium (after passing the simulated GIT). The cell wall thickness in the DDG medium was the highest (68%-p≤0.0001). Infrared spectra had a similar spectral pattern for all treatments, however, bands from DDG medium had greater absorption intensity than those obtained from YPD and in turn, increased after the GI tract passage. S. cerevisiae RC016 showed important AFB1 adsorption in the simulated intestinal fluid (97.7% in DDG). The optimization of nutritional conditions coupled with the use of spectrophotometric tools allowed increasing, in a controlled manner, the main components of the cell wall responsible for adsorbing mycotoxins simulating the GI tract. Consequently, it could be possible to control the functional properties (probiotic and mycotoxin adsorbents) after scaling the yeast biomass production with low-cost substrates, allowing its effective application as a food additive.
Fil: Fochesato, Analía Silvia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Poloni, Valeria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina
Fil: Dogi, Cecilia Ana. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
AFB1 ADSORPTION
INFRARED SPECTROSCOPY
SIMULATED GASTROINTESTINAL TRACT
TRANSMISSION ELECTRON MICROSCOPY
YEAST CELL WALL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138657
Acceder
id CONICETDig_daa8ed29da3a95a1fe0379566b65f814
oai_identifier_str oai:ri.conicet.gov.ar:11336/138657
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorptionFochesato, Analía SilviaCristofolini, Andrea LorenaPoloni, Valeria LorenaMagnoli, Alejandra PaolaMerkis, Cecilia InésDogi, Cecilia AnaCavaglieri, Lilia ReneéAFB1 ADSORPTIONINFRARED SPECTROSCOPYSIMULATED GASTROINTESTINAL TRACTTRANSMISSION ELECTRON MICROSCOPYYEAST CELL WALLhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The objective was to study the influence of culture medium and gastrointestinal environment on Saccharomyces cerevisiae RC016 cell wall polysaccharides profile and the aflatoxin B1 (AFB1) adsorption. Probiotic Saccharomyces cerevisiae RC016, isolated from the pig small intestine, has previously shown efficient mycotoxins adsorption in vitro including AFB1. In addition, it was able to survive under the gastrointestinal (GI) tract conditions and did not cause in vitro and in vivo genotoxicity or cytotoxicity. In this work, transmission electron microscopy (TEM) was used to show ultrastructural variations in cell morphology and, infrared spectroscopy (IR-frequency range 4000–500 cm−1) was used to show variations in the spectra of yeast cells cultured in different culture media, Yeast extract Peptone Dextrose (YPD) medium and Dried distillers grains (DDG) medium (after passing the simulated GIT). The cell wall thickness in the DDG medium was the highest (68%-p≤0.0001). Infrared spectra had a similar spectral pattern for all treatments, however, bands from DDG medium had greater absorption intensity than those obtained from YPD and in turn, increased after the GI tract passage. S. cerevisiae RC016 showed important AFB1 adsorption in the simulated intestinal fluid (97.7% in DDG). The optimization of nutritional conditions coupled with the use of spectrophotometric tools allowed increasing, in a controlled manner, the main components of the cell wall responsible for adsorbing mycotoxins simulating the GI tract. Consequently, it could be possible to control the functional properties (probiotic and mycotoxin adsorbents) after scaling the yeast biomass production with low-cost substrates, allowing its effective application as a food additive.Fil: Fochesato, Analía Silvia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cristofolini, Andrea Lorena. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; ArgentinaFil: Poloni, Valeria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; ArgentinaFil: Magnoli, Alejandra Paola. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; ArgentinaFil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; ArgentinaFil: Dogi, Cecilia Ana. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138657Fochesato, Analía Silvia; Cristofolini, Andrea Lorena; Poloni, Valeria Lorena; Magnoli, Alejandra Paola; Merkis, Cecilia Inés; et al.; Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption; Elsevier Science; LWT - Food Science and Technology; 126; 5-2020; 1-70023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643820302954info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2020.109306info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:04:42Zoai:ri.conicet.gov.ar:11336/138657instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:04:42.776CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
title Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
spellingShingle Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
Fochesato, Analía Silvia
AFB1 ADSORPTION
INFRARED SPECTROSCOPY
SIMULATED GASTROINTESTINAL TRACT
TRANSMISSION ELECTRON MICROSCOPY
YEAST CELL WALL
title_short Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
title_full Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
title_fullStr Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
title_full_unstemmed Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
title_sort Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption
dc.creator.none.fl_str_mv Fochesato, Analía Silvia
Cristofolini, Andrea Lorena
Poloni, Valeria Lorena
Magnoli, Alejandra Paola
Merkis, Cecilia Inés
Dogi, Cecilia Ana
Cavaglieri, Lilia Reneé
author Fochesato, Analía Silvia
author_facet Fochesato, Analía Silvia
Cristofolini, Andrea Lorena
Poloni, Valeria Lorena
Magnoli, Alejandra Paola
Merkis, Cecilia Inés
Dogi, Cecilia Ana
Cavaglieri, Lilia Reneé
author_role author
author2 Cristofolini, Andrea Lorena
Poloni, Valeria Lorena
Magnoli, Alejandra Paola
Merkis, Cecilia Inés
Dogi, Cecilia Ana
Cavaglieri, Lilia Reneé
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AFB1 ADSORPTION
INFRARED SPECTROSCOPY
SIMULATED GASTROINTESTINAL TRACT
TRANSMISSION ELECTRON MICROSCOPY
YEAST CELL WALL
topic AFB1 ADSORPTION
INFRARED SPECTROSCOPY
SIMULATED GASTROINTESTINAL TRACT
TRANSMISSION ELECTRON MICROSCOPY
YEAST CELL WALL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective was to study the influence of culture medium and gastrointestinal environment on Saccharomyces cerevisiae RC016 cell wall polysaccharides profile and the aflatoxin B1 (AFB1) adsorption. Probiotic Saccharomyces cerevisiae RC016, isolated from the pig small intestine, has previously shown efficient mycotoxins adsorption in vitro including AFB1. In addition, it was able to survive under the gastrointestinal (GI) tract conditions and did not cause in vitro and in vivo genotoxicity or cytotoxicity. In this work, transmission electron microscopy (TEM) was used to show ultrastructural variations in cell morphology and, infrared spectroscopy (IR-frequency range 4000–500 cm−1) was used to show variations in the spectra of yeast cells cultured in different culture media, Yeast extract Peptone Dextrose (YPD) medium and Dried distillers grains (DDG) medium (after passing the simulated GIT). The cell wall thickness in the DDG medium was the highest (68%-p≤0.0001). Infrared spectra had a similar spectral pattern for all treatments, however, bands from DDG medium had greater absorption intensity than those obtained from YPD and in turn, increased after the GI tract passage. S. cerevisiae RC016 showed important AFB1 adsorption in the simulated intestinal fluid (97.7% in DDG). The optimization of nutritional conditions coupled with the use of spectrophotometric tools allowed increasing, in a controlled manner, the main components of the cell wall responsible for adsorbing mycotoxins simulating the GI tract. Consequently, it could be possible to control the functional properties (probiotic and mycotoxin adsorbents) after scaling the yeast biomass production with low-cost substrates, allowing its effective application as a food additive.
Fil: Fochesato, Analía Silvia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Poloni, Valeria Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina
Fil: Dogi, Cecilia Ana. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The objective was to study the influence of culture medium and gastrointestinal environment on Saccharomyces cerevisiae RC016 cell wall polysaccharides profile and the aflatoxin B1 (AFB1) adsorption. Probiotic Saccharomyces cerevisiae RC016, isolated from the pig small intestine, has previously shown efficient mycotoxins adsorption in vitro including AFB1. In addition, it was able to survive under the gastrointestinal (GI) tract conditions and did not cause in vitro and in vivo genotoxicity or cytotoxicity. In this work, transmission electron microscopy (TEM) was used to show ultrastructural variations in cell morphology and, infrared spectroscopy (IR-frequency range 4000–500 cm−1) was used to show variations in the spectra of yeast cells cultured in different culture media, Yeast extract Peptone Dextrose (YPD) medium and Dried distillers grains (DDG) medium (after passing the simulated GIT). The cell wall thickness in the DDG medium was the highest (68%-p≤0.0001). Infrared spectra had a similar spectral pattern for all treatments, however, bands from DDG medium had greater absorption intensity than those obtained from YPD and in turn, increased after the GI tract passage. S. cerevisiae RC016 showed important AFB1 adsorption in the simulated intestinal fluid (97.7% in DDG). The optimization of nutritional conditions coupled with the use of spectrophotometric tools allowed increasing, in a controlled manner, the main components of the cell wall responsible for adsorbing mycotoxins simulating the GI tract. Consequently, it could be possible to control the functional properties (probiotic and mycotoxin adsorbents) after scaling the yeast biomass production with low-cost substrates, allowing its effective application as a food additive.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138657
Fochesato, Analía Silvia; Cristofolini, Andrea Lorena; Poloni, Valeria Lorena; Magnoli, Alejandra Paola; Merkis, Cecilia Inés; et al.; Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption; Elsevier Science; LWT - Food Science and Technology; 126; 5-2020; 1-7
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138657
identifier_str_mv Fochesato, Analía Silvia; Cristofolini, Andrea Lorena; Poloni, Valeria Lorena; Magnoli, Alejandra Paola; Merkis, Cecilia Inés; et al.; Culture medium and gastrointestinal environment positively influence the Saccharomyces cerevisiae RC016 cell wall polysaccharide profile and aflatoxin B1 bioadsorption; Elsevier Science; LWT - Food Science and Technology; 126; 5-2020; 1-7
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643820302954
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2020.109306
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847426828038307840
score 13.10058

Publicaciones similares