Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)

Autores
Almandoz, Gaston Osvaldo; Montoya, Nora Gladys; Hernando, Marcelo Pablo; Benavides, Hugo R.; Carignan, Mario O.; Ferrario, Martha Elba
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During phytoplankton monitoring in the Beagle Channel (≈54°52′ S, 67°32′ W) a previously undetected Alexandrium species was observed in coincidence with mouse bioassay toxicity. Detailed thecal plates analysis using epifluorescence and scanning electron microscopy revealed the presence of the Alexandrium ostenfeldii species complex, showing a mixture of the diagnostic features usually used to discriminate between the morphospecies A. ostenfeldii and A. peruvianum. Cells of the A. ostenfeldii complex were commonly observed during spring after the main annual diatom bloom, when temperatures and salinities were respectively around 7.5–10 °C and 30–30.5 psu, and nutrients showed a seasonal decrease. Toxin analysis by liquid chromatography–mass spectrometry revealed the production of 13-desmethyl spirolide C and 20-methyl spirolide G in cell cultures. The cellular contain of spirolides during exponential phase growth was 0.5906 ± 0.0032 and 0.1577 ± 0.0023 pg cell−1 for 13-desMe-C and 20-Me-G, respectively. A third unknown compound, with a structure resembling that of spirolides was also detected in culture. Moreover, an additional compound with a similar m/z (692) than that of 13-desMe-C but presenting a higher retention time (Rt = 40.5 min) was found in high proportions in mussel samples. PSP toxins were present at low concentration in mussels but were not detected in cultures. These results extend the world-wide distribution of toxic strains of the A. ostenfeldii complex to the Beagle Channel (southern South America), where toxic events have been traditionally linked to the presence of Alexandrium catenella. This is the first confirmed occurrence of spirolides in mussels and plankton from Argentina, which highlights the importance of monitoring these toxins and their producing organisms to protect public health and improve the management of shellfish resources.
Fil: Almandoz, Gaston Osvaldo. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montoya, Nora Gladys. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Hernando, Marcelo Pablo. Comisión Nacional de Energía Atómica; Argentina
Fil: Benavides, Hugo R.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Carignan, Mario O.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Ferrario, Martha Elba. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Ecology
Morphology
Mussels
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33997
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33997
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)Almandoz, Gaston OsvaldoMontoya, Nora GladysHernando, Marcelo PabloBenavides, Hugo R.Carignan, Mario O.Ferrario, Martha ElbaEcologyMorphologyMusselshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During phytoplankton monitoring in the Beagle Channel (≈54°52′ S, 67°32′ W) a previously undetected Alexandrium species was observed in coincidence with mouse bioassay toxicity. Detailed thecal plates analysis using epifluorescence and scanning electron microscopy revealed the presence of the Alexandrium ostenfeldii species complex, showing a mixture of the diagnostic features usually used to discriminate between the morphospecies A. ostenfeldii and A. peruvianum. Cells of the A. ostenfeldii complex were commonly observed during spring after the main annual diatom bloom, when temperatures and salinities were respectively around 7.5–10 °C and 30–30.5 psu, and nutrients showed a seasonal decrease. Toxin analysis by liquid chromatography–mass spectrometry revealed the production of 13-desmethyl spirolide C and 20-methyl spirolide G in cell cultures. The cellular contain of spirolides during exponential phase growth was 0.5906 ± 0.0032 and 0.1577 ± 0.0023 pg cell−1 for 13-desMe-C and 20-Me-G, respectively. A third unknown compound, with a structure resembling that of spirolides was also detected in culture. Moreover, an additional compound with a similar m/z (692) than that of 13-desMe-C but presenting a higher retention time (Rt = 40.5 min) was found in high proportions in mussel samples. PSP toxins were present at low concentration in mussels but were not detected in cultures. These results extend the world-wide distribution of toxic strains of the A. ostenfeldii complex to the Beagle Channel (southern South America), where toxic events have been traditionally linked to the presence of Alexandrium catenella. This is the first confirmed occurrence of spirolides in mussels and plankton from Argentina, which highlights the importance of monitoring these toxins and their producing organisms to protect public health and improve the management of shellfish resources.Fil: Almandoz, Gaston Osvaldo. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montoya, Nora Gladys. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Hernando, Marcelo Pablo. Comisión Nacional de Energía Atómica; ArgentinaFil: Benavides, Hugo R.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Carignan, Mario O.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Ferrario, Martha Elba. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33997Almandoz, Gaston Osvaldo; Montoya, Nora Gladys; Hernando, Marcelo Pablo; Benavides, Hugo R.; Carignan, Mario O.; et al.; Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina); Elsevier Science; Harmful Algae; 37; 6-2014; 100-1091568-9883CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.hal.2014.05.011info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S156898831400095Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:18:44Zoai:ri.conicet.gov.ar:11336/33997instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:18:44.822CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
title Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
spellingShingle Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
Almandoz, Gaston Osvaldo
Ecology
Morphology
Mussels
title_short Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
title_full Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
title_fullStr Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
title_full_unstemmed Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
title_sort Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina)
dc.creator.none.fl_str_mv Almandoz, Gaston Osvaldo
Montoya, Nora Gladys
Hernando, Marcelo Pablo
Benavides, Hugo R.
Carignan, Mario O.
Ferrario, Martha Elba
author Almandoz, Gaston Osvaldo
author_facet Almandoz, Gaston Osvaldo
Montoya, Nora Gladys
Hernando, Marcelo Pablo
Benavides, Hugo R.
Carignan, Mario O.
Ferrario, Martha Elba
author_role author
author2 Montoya, Nora Gladys
Hernando, Marcelo Pablo
Benavides, Hugo R.
Carignan, Mario O.
Ferrario, Martha Elba
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ecology
Morphology
Mussels
topic Ecology
Morphology
Mussels
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During phytoplankton monitoring in the Beagle Channel (≈54°52′ S, 67°32′ W) a previously undetected Alexandrium species was observed in coincidence with mouse bioassay toxicity. Detailed thecal plates analysis using epifluorescence and scanning electron microscopy revealed the presence of the Alexandrium ostenfeldii species complex, showing a mixture of the diagnostic features usually used to discriminate between the morphospecies A. ostenfeldii and A. peruvianum. Cells of the A. ostenfeldii complex were commonly observed during spring after the main annual diatom bloom, when temperatures and salinities were respectively around 7.5–10 °C and 30–30.5 psu, and nutrients showed a seasonal decrease. Toxin analysis by liquid chromatography–mass spectrometry revealed the production of 13-desmethyl spirolide C and 20-methyl spirolide G in cell cultures. The cellular contain of spirolides during exponential phase growth was 0.5906 ± 0.0032 and 0.1577 ± 0.0023 pg cell−1 for 13-desMe-C and 20-Me-G, respectively. A third unknown compound, with a structure resembling that of spirolides was also detected in culture. Moreover, an additional compound with a similar m/z (692) than that of 13-desMe-C but presenting a higher retention time (Rt = 40.5 min) was found in high proportions in mussel samples. PSP toxins were present at low concentration in mussels but were not detected in cultures. These results extend the world-wide distribution of toxic strains of the A. ostenfeldii complex to the Beagle Channel (southern South America), where toxic events have been traditionally linked to the presence of Alexandrium catenella. This is the first confirmed occurrence of spirolides in mussels and plankton from Argentina, which highlights the importance of monitoring these toxins and their producing organisms to protect public health and improve the management of shellfish resources.
Fil: Almandoz, Gaston Osvaldo. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montoya, Nora Gladys. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Hernando, Marcelo Pablo. Comisión Nacional de Energía Atómica; Argentina
Fil: Benavides, Hugo R.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Carignan, Mario O.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Ferrario, Martha Elba. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Division Ficología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description During phytoplankton monitoring in the Beagle Channel (≈54°52′ S, 67°32′ W) a previously undetected Alexandrium species was observed in coincidence with mouse bioassay toxicity. Detailed thecal plates analysis using epifluorescence and scanning electron microscopy revealed the presence of the Alexandrium ostenfeldii species complex, showing a mixture of the diagnostic features usually used to discriminate between the morphospecies A. ostenfeldii and A. peruvianum. Cells of the A. ostenfeldii complex were commonly observed during spring after the main annual diatom bloom, when temperatures and salinities were respectively around 7.5–10 °C and 30–30.5 psu, and nutrients showed a seasonal decrease. Toxin analysis by liquid chromatography–mass spectrometry revealed the production of 13-desmethyl spirolide C and 20-methyl spirolide G in cell cultures. The cellular contain of spirolides during exponential phase growth was 0.5906 ± 0.0032 and 0.1577 ± 0.0023 pg cell−1 for 13-desMe-C and 20-Me-G, respectively. A third unknown compound, with a structure resembling that of spirolides was also detected in culture. Moreover, an additional compound with a similar m/z (692) than that of 13-desMe-C but presenting a higher retention time (Rt = 40.5 min) was found in high proportions in mussel samples. PSP toxins were present at low concentration in mussels but were not detected in cultures. These results extend the world-wide distribution of toxic strains of the A. ostenfeldii complex to the Beagle Channel (southern South America), where toxic events have been traditionally linked to the presence of Alexandrium catenella. This is the first confirmed occurrence of spirolides in mussels and plankton from Argentina, which highlights the importance of monitoring these toxins and their producing organisms to protect public health and improve the management of shellfish resources.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33997
Almandoz, Gaston Osvaldo; Montoya, Nora Gladys; Hernando, Marcelo Pablo; Benavides, Hugo R.; Carignan, Mario O.; et al.; Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina); Elsevier Science; Harmful Algae; 37; 6-2014; 100-109
1568-9883
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33997
identifier_str_mv Almandoz, Gaston Osvaldo; Montoya, Nora Gladys; Hernando, Marcelo Pablo; Benavides, Hugo R.; Carignan, Mario O.; et al.; Toxic strains of the Alexandrium ostenfeldii complex in southern South America (Beagle Channel, Argentina); Elsevier Science; Harmful Algae; 37; 6-2014; 100-109
1568-9883
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.hal.2014.05.011
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S156898831400095X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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