Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solid...

Autores
Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; Parker, J. J.; Sayler, G. S.
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Layton, A. C.. University of Tennessee; Estados Unidos
Fil: Robinson, K. G.. University of Tennessee; Estados Unidos
Fil: Brown, J. R.. University of Tennessee; Estados Unidos
Fil: Gregory, I. R.. University of Tennessee; Estados Unidos
Fil: Parker, J. J.. University of Tennessee; Estados Unidos
Fil: Sayler, G. S.. University of Tennessee; Estados Unidos
Materia
COMPETITIVE POLYMERASE CHAIN REACTION
NITROSOMONAS
NITROSPIRA
amoA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99515
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99515
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention TimesDionisi, Hebe MonicaLayton, A. C.Robinson, K. G.Brown, J. R.Gregory, I. R.Parker, J. J.Sayler, G. S.COMPETITIVE POLYMERASE CHAIN REACTIONNITROSOMONASNITROSPIRAamoAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Layton, A. C.. University of Tennessee; Estados UnidosFil: Robinson, K. G.. University of Tennessee; Estados UnidosFil: Brown, J. R.. University of Tennessee; Estados UnidosFil: Gregory, I. R.. University of Tennessee; Estados UnidosFil: Parker, J. J.. University of Tennessee; Estados UnidosFil: Sayler, G. S.. University of Tennessee; Estados UnidosWiley2002-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99515Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; et al.; Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times; Wiley; Water Environment Research; 74; 5; 9-2002; 462-4691061-4303CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2175/106143002x144815info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.2175/106143002X144815info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:46Zoai:ri.conicet.gov.ar:11336/99515instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:46.335CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
spellingShingle Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
Dionisi, Hebe Monica
COMPETITIVE POLYMERASE CHAIN REACTION
NITROSOMONAS
NITROSPIRA
amoA
title_short Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_full Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_fullStr Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_full_unstemmed Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_sort Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
dc.creator.none.fl_str_mv Dionisi, Hebe Monica
Layton, A. C.
Robinson, K. G.
Brown, J. R.
Gregory, I. R.
Parker, J. J.
Sayler, G. S.
author Dionisi, Hebe Monica
author_facet Dionisi, Hebe Monica
Layton, A. C.
Robinson, K. G.
Brown, J. R.
Gregory, I. R.
Parker, J. J.
Sayler, G. S.
author_role author
author2 Layton, A. C.
Robinson, K. G.
Brown, J. R.
Gregory, I. R.
Parker, J. J.
Sayler, G. S.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv COMPETITIVE POLYMERASE CHAIN REACTION
NITROSOMONAS
NITROSPIRA
amoA
topic COMPETITIVE POLYMERASE CHAIN REACTION
NITROSOMONAS
NITROSPIRA
amoA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Layton, A. C.. University of Tennessee; Estados Unidos
Fil: Robinson, K. G.. University of Tennessee; Estados Unidos
Fil: Brown, J. R.. University of Tennessee; Estados Unidos
Fil: Gregory, I. R.. University of Tennessee; Estados Unidos
Fil: Parker, J. J.. University of Tennessee; Estados Unidos
Fil: Sayler, G. S.. University of Tennessee; Estados Unidos
description The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.
publishDate 2002
dc.date.none.fl_str_mv 2002-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99515
Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; et al.; Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times; Wiley; Water Environment Research; 74; 5; 9-2002; 462-469
1061-4303
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99515
identifier_str_mv Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; et al.; Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times; Wiley; Water Environment Research; 74; 5; 9-2002; 462-469
1061-4303
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2175/106143002x144815
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.2175/106143002X144815
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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