Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solid...

Autores: Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; Parker, J. J.; Sayler, G. S.
Año de publicación: 2002
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Layton, A. C.. University of Tennessee; Estados Unidos
Fil: Robinson, K. G.. University of Tennessee; Estados Unidos
Fil: Brown, J. R.. University of Tennessee; Estados Unidos
Fil: Gregory, I. R.. University of Tennessee; Estados Unidos
Fil: Parker, J. J.. University of Tennessee; Estados Unidos
Fil: Sayler, G. S.. University of Tennessee; Estados Unidos
Materia: COMPETITIVE POLYMERASE CHAIN REACTION
NITROSOMONAS
NITROSPIRA
amoA
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/99515

Acceder

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention TimesDionisi, Hebe MonicaLayton, A. C.Robinson, K. G.Brown, J. R.Gregory, I. R.Parker, J. J.Sayler, G. S.COMPETITIVE POLYMERASE CHAIN REACTIONNITROSOMONASNITROSPIRAamoAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Layton, A. C.. University of Tennessee; Estados UnidosFil: Robinson, K. G.. University of Tennessee; Estados UnidosFil: Brown, J. R.. University of Tennessee; Estados UnidosFil: Gregory, I. R.. University of Tennessee; Estados UnidosFil: Parker, J. J.. University of Tennessee; Estados UnidosFil: Sayler, G. S.. University of Tennessee; Estados UnidosWiley2002-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99515Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; et al.; Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times; Wiley; Water Environment Research; 74; 5; 9-2002; 462-4691061-4303CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2175/106143002x144815info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.2175/106143002X144815info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:31Zoai:ri.conicet.gov.ar:11336/99515instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:32.45CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
spellingShingle	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times Dionisi, Hebe Monica COMPETITIVE POLYMERASE CHAIN REACTION NITROSOMONAS NITROSPIRA amoA
title_short	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_full	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_fullStr	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_full_unstemmed	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
title_sort	Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times
dc.creator.none.fl_str_mv	Dionisi, Hebe Monica Layton, A. C. Robinson, K. G. Brown, J. R. Gregory, I. R. Parker, J. J. Sayler, G. S.
author	Dionisi, Hebe Monica
author_facet	Dionisi, Hebe Monica Layton, A. C. Robinson, K. G. Brown, J. R. Gregory, I. R. Parker, J. J. Sayler, G. S.
author_role	author
author2	Layton, A. C. Robinson, K. G. Brown, J. R. Gregory, I. R. Parker, J. J. Sayler, G. S.
author2_role	author author author author author author
dc.subject.none.fl_str_mv	COMPETITIVE POLYMERASE CHAIN REACTION NITROSOMONAS NITROSPIRA amoA
topic	COMPETITIVE POLYMERASE CHAIN REACTION NITROSOMONAS NITROSPIRA amoA
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems. Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina Fil: Layton, A. C.. University of Tennessee; Estados Unidos Fil: Robinson, K. G.. University of Tennessee; Estados Unidos Fil: Brown, J. R.. University of Tennessee; Estados Unidos Fil: Gregory, I. R.. University of Tennessee; Estados Unidos Fil: Parker, J. J.. University of Tennessee; Estados Unidos Fil: Sayler, G. S.. University of Tennessee; Estados Unidos
description	The effect of solids retention time (SRT) on ammonia and nitrite‐oxidizing bacteria was measured by Nitrosomonas oligotropha‐like ammonia monooxygenase A and Nitrospira 16S rDNA competitive polymerase chain reaction assays in a complete‐mix, bench‐scale, activated‐sludge system. During steady‐state operation, nitrification was complete in the 20‐ and 10‐day SRT reactors, nearly complete in the 5‐day SRT reactor, and incomplete in the 2‐day SRT reactor (76% ammonia oxidation and 85% nitrite oxidation). Total microbes, measured by dot‐blot hybridizations, ranged from 3 × 1011 to 3 × 1012 cells/L, and increased with increasing SRTs. The concentration of the ammonia‐oxidizer N. oligotropha dropped 100‐fold from the 20‐day SRT (5 × 109 cells/L) to the 2‐day SRT (≤4 × 107 cells/L). Thus, N. oligotropha became a much smaller fraction of the total biomass in the poorly performing 2‐day SRT reactor. The concentration of Nitrospira cells also decreased (10‐fold) as the SRT was reduced from 20 days to 2 days. However, the number of Nitrospira cells was always greater than the number of N. oligotropha cells measured in each reactor (10‐ to 60‐fold). While Nitrospira comprised 1 to 2% of the biomass, N. oligotropha represented only 0.04 to 0.27% of the total population. This low percentage suggests that N. oligotropha was not a dominant ammonia oxidizer in the bench‐scale systems.
publishDate	2002
dc.date.none.fl_str_mv	2002-09
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/99515 Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; et al.; Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times; Wiley; Water Environment Research; 74; 5; 9-2002; 462-469 1061-4303 CONICET Digital CONICET
url	http://hdl.handle.net/11336/99515
identifier_str_mv	Dionisi, Hebe Monica; Layton, A. C.; Robinson, K. G.; Brown, J. R.; Gregory, I. R.; et al.; Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solids Retention Times; Wiley; Water Environment Research; 74; 5; 9-2002; 462-469 1061-4303 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.2175/106143002x144815 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.2175/106143002X144815
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Wiley
publisher.none.fl_str_mv	Wiley
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Quantification of Nitrosomonas oligotropha and Nitrospira spp. Using Competitive Polymerase Chain Reaction in Bench-Scale Wastewater Treatment Reactors Operating at Different Solid...

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