mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts

Autores
Caruso, Vanni; Sheedharan, Smitha; Carlini, Valeria Paola; Jacobsson, Josefin A.; Haitina, Tatjana; Hammer, Joanna; Stephansson, Olga; Crona, Filip; Sommer, Wolfgang; Risérus, Ulf; Lannfelt, Lars; Marcus, Claude; Heiling, Markus; Rubiales, Susana Elizabeth; Fredriksson, Robert; Schiöth, Helgi B.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating intercellular interactions of fundamental physiological importance for survival including regulation of food intake, blood pressure, and hormonal sensing signaling, among other roles. Homeostatic alterations in the physiological status of GPCRs are often associated with underlying causes of disease, and to date, several orphan GPCRs are still uncharacterized.Findings from our previous study demonstrate that the Rhodopsin family protein GPR162 is widely expressed in GABAergic as well as other neurons within the mouse hippocampus, whereas extensive expression is observed in hypothalamus, amygdala, and ventral tegmental area, regions strictly interconnected and involved in the regulation of energy homeostasis and hedonic feeding.In this study, we provide a further anatomical characterization of GPR162 in mouse brain via in situ hybridization as well as detailed mRNA expression in a panel of rat tissues complementing a specie-specific mapping of the receptor. We also provide an attempt to demonstrate a functional implication of GPR162 in food intake-related behavior via antisense knockdown studies. Furthermore, we performed human genetic studies in which for the first time, variants of the GPR162 gene were associated with impairments in glucose homeostasis.
Fil: Caruso, Vanni. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. University of Tasmania; Australia
Fil: Sheedharan, Smitha. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Carlini, Valeria Paola. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Jacobsson, Josefin A.. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Haitina, Tatjana. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Hammer, Joanna. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Stephansson, Olga. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Crona, Filip. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Sommer, Wolfgang. Central Institute of Mental Health. Department of Psychopharmacology; Alemania
Fil: Risérus, Ulf. Uppsala University. Clinical Nutrition and Metabolism. Department of Public Health and Caring Sciences; Suecia
Fil: Lannfelt, Lars. Department Of Neuroscience, Functional Pharmacology; Suecia. Uppsala University. Department of Public Health and Caring Sciences, Geriatrics; Suecia
Fil: Marcus, Claude. Department Of Neuroscience, Functional Pharmacology; Suecia. Karolinska Institute. National Childhood Obesity Centre. Department for Clinical Science, Intervention and Technology. Division of Pediatrics; Suecia
Fil: Heiling, Markus. National Institutes of Health. National Institute on Alcohol Abuse and Alcoholism. Laboratory of Clinical and Translational Studies; Estados Unidos
Fil: Rubiales, Susana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Fredriksson, Robert. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; Suecia
Fil: Schiöth, Helgi B.. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; Suecia
Materia
Food Intake
Glucose Homeostasis
Gpcrs
Gpr162
Obesity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50643
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50643
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohortsCaruso, VanniSheedharan, SmithaCarlini, Valeria PaolaJacobsson, Josefin A.Haitina, TatjanaHammer, JoannaStephansson, OlgaCrona, FilipSommer, WolfgangRisérus, UlfLannfelt, LarsMarcus, ClaudeHeiling, MarkusRubiales, Susana ElizabethFredriksson, RobertSchiöth, Helgi B.Food IntakeGlucose HomeostasisGpcrsGpr162Obesityhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating intercellular interactions of fundamental physiological importance for survival including regulation of food intake, blood pressure, and hormonal sensing signaling, among other roles. Homeostatic alterations in the physiological status of GPCRs are often associated with underlying causes of disease, and to date, several orphan GPCRs are still uncharacterized.Findings from our previous study demonstrate that the Rhodopsin family protein GPR162 is widely expressed in GABAergic as well as other neurons within the mouse hippocampus, whereas extensive expression is observed in hypothalamus, amygdala, and ventral tegmental area, regions strictly interconnected and involved in the regulation of energy homeostasis and hedonic feeding.In this study, we provide a further anatomical characterization of GPR162 in mouse brain via in situ hybridization as well as detailed mRNA expression in a panel of rat tissues complementing a specie-specific mapping of the receptor. We also provide an attempt to demonstrate a functional implication of GPR162 in food intake-related behavior via antisense knockdown studies. Furthermore, we performed human genetic studies in which for the first time, variants of the GPR162 gene were associated with impairments in glucose homeostasis.Fil: Caruso, Vanni. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. University of Tasmania; AustraliaFil: Sheedharan, Smitha. Uppsala University. Department Of Neuroscience. Functional Pharmacology; SueciaFil: Carlini, Valeria Paola. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Jacobsson, Josefin A.. Uppsala University. Department Of Neuroscience. Functional Pharmacology; SueciaFil: Haitina, Tatjana. Uppsala University. Department Of Neuroscience. Functional Pharmacology; SueciaFil: Hammer, Joanna. Uppsala University. Department Of Neuroscience. Functional Pharmacology; SueciaFil: Stephansson, Olga. Uppsala University. Department Of Neuroscience. Functional Pharmacology; SueciaFil: Crona, Filip. Uppsala University. Department Of Neuroscience. Functional Pharmacology; SueciaFil: Sommer, Wolfgang. Central Institute of Mental Health. Department of Psychopharmacology; AlemaniaFil: Risérus, Ulf. Uppsala University. Clinical Nutrition and Metabolism. Department of Public Health and Caring Sciences; SueciaFil: Lannfelt, Lars. Department Of Neuroscience, Functional Pharmacology; Suecia. Uppsala University. Department of Public Health and Caring Sciences, Geriatrics; SueciaFil: Marcus, Claude. Department Of Neuroscience, Functional Pharmacology; Suecia. Karolinska Institute. National Childhood Obesity Centre. Department for Clinical Science, Intervention and Technology. Division of Pediatrics; SueciaFil: Heiling, Markus. National Institutes of Health. National Institute on Alcohol Abuse and Alcoholism. Laboratory of Clinical and Translational Studies; Estados UnidosFil: Rubiales, Susana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Fredriksson, Robert. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; SueciaFil: Schiöth, Helgi B.. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; SueciaElsevier Science2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50643Caruso, Vanni; Sheedharan, Smitha; Carlini, Valeria Paola; Jacobsson, Josefin A.; Haitina, Tatjana; et al.; mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts; Elsevier Science; Gene; 581; 2; 5-2016; 139-1450378-1119CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111916001062info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2016.01.044info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:11Zoai:ri.conicet.gov.ar:11336/50643instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:11.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
title mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
spellingShingle mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
Caruso, Vanni
Food Intake
Glucose Homeostasis
Gpcrs
Gpr162
Obesity
title_short mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
title_full mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
title_fullStr mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
title_full_unstemmed mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
title_sort mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
dc.creator.none.fl_str_mv Caruso, Vanni
Sheedharan, Smitha
Carlini, Valeria Paola
Jacobsson, Josefin A.
Haitina, Tatjana
Hammer, Joanna
Stephansson, Olga
Crona, Filip
Sommer, Wolfgang
Risérus, Ulf
Lannfelt, Lars
Marcus, Claude
Heiling, Markus
Rubiales, Susana Elizabeth
Fredriksson, Robert
Schiöth, Helgi B.
author Caruso, Vanni
author_facet Caruso, Vanni
Sheedharan, Smitha
Carlini, Valeria Paola
Jacobsson, Josefin A.
Haitina, Tatjana
Hammer, Joanna
Stephansson, Olga
Crona, Filip
Sommer, Wolfgang
Risérus, Ulf
Lannfelt, Lars
Marcus, Claude
Heiling, Markus
Rubiales, Susana Elizabeth
Fredriksson, Robert
Schiöth, Helgi B.
author_role author
author2 Sheedharan, Smitha
Carlini, Valeria Paola
Jacobsson, Josefin A.
Haitina, Tatjana
Hammer, Joanna
Stephansson, Olga
Crona, Filip
Sommer, Wolfgang
Risérus, Ulf
Lannfelt, Lars
Marcus, Claude
Heiling, Markus
Rubiales, Susana Elizabeth
Fredriksson, Robert
Schiöth, Helgi B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Food Intake
Glucose Homeostasis
Gpcrs
Gpr162
Obesity
topic Food Intake
Glucose Homeostasis
Gpcrs
Gpr162
Obesity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating intercellular interactions of fundamental physiological importance for survival including regulation of food intake, blood pressure, and hormonal sensing signaling, among other roles. Homeostatic alterations in the physiological status of GPCRs are often associated with underlying causes of disease, and to date, several orphan GPCRs are still uncharacterized.Findings from our previous study demonstrate that the Rhodopsin family protein GPR162 is widely expressed in GABAergic as well as other neurons within the mouse hippocampus, whereas extensive expression is observed in hypothalamus, amygdala, and ventral tegmental area, regions strictly interconnected and involved in the regulation of energy homeostasis and hedonic feeding.In this study, we provide a further anatomical characterization of GPR162 in mouse brain via in situ hybridization as well as detailed mRNA expression in a panel of rat tissues complementing a specie-specific mapping of the receptor. We also provide an attempt to demonstrate a functional implication of GPR162 in food intake-related behavior via antisense knockdown studies. Furthermore, we performed human genetic studies in which for the first time, variants of the GPR162 gene were associated with impairments in glucose homeostasis.
Fil: Caruso, Vanni. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. University of Tasmania; Australia
Fil: Sheedharan, Smitha. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Carlini, Valeria Paola. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Jacobsson, Josefin A.. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Haitina, Tatjana. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Hammer, Joanna. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Stephansson, Olga. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Crona, Filip. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia
Fil: Sommer, Wolfgang. Central Institute of Mental Health. Department of Psychopharmacology; Alemania
Fil: Risérus, Ulf. Uppsala University. Clinical Nutrition and Metabolism. Department of Public Health and Caring Sciences; Suecia
Fil: Lannfelt, Lars. Department Of Neuroscience, Functional Pharmacology; Suecia. Uppsala University. Department of Public Health and Caring Sciences, Geriatrics; Suecia
Fil: Marcus, Claude. Department Of Neuroscience, Functional Pharmacology; Suecia. Karolinska Institute. National Childhood Obesity Centre. Department for Clinical Science, Intervention and Technology. Division of Pediatrics; Suecia
Fil: Heiling, Markus. National Institutes of Health. National Institute on Alcohol Abuse and Alcoholism. Laboratory of Clinical and Translational Studies; Estados Unidos
Fil: Rubiales, Susana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Fredriksson, Robert. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; Suecia
Fil: Schiöth, Helgi B.. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; Suecia
description G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating intercellular interactions of fundamental physiological importance for survival including regulation of food intake, blood pressure, and hormonal sensing signaling, among other roles. Homeostatic alterations in the physiological status of GPCRs are often associated with underlying causes of disease, and to date, several orphan GPCRs are still uncharacterized.Findings from our previous study demonstrate that the Rhodopsin family protein GPR162 is widely expressed in GABAergic as well as other neurons within the mouse hippocampus, whereas extensive expression is observed in hypothalamus, amygdala, and ventral tegmental area, regions strictly interconnected and involved in the regulation of energy homeostasis and hedonic feeding.In this study, we provide a further anatomical characterization of GPR162 in mouse brain via in situ hybridization as well as detailed mRNA expression in a panel of rat tissues complementing a specie-specific mapping of the receptor. We also provide an attempt to demonstrate a functional implication of GPR162 in food intake-related behavior via antisense knockdown studies. Furthermore, we performed human genetic studies in which for the first time, variants of the GPR162 gene were associated with impairments in glucose homeostasis.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50643
Caruso, Vanni; Sheedharan, Smitha; Carlini, Valeria Paola; Jacobsson, Josefin A.; Haitina, Tatjana; et al.; mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts; Elsevier Science; Gene; 581; 2; 5-2016; 139-145
0378-1119
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50643
identifier_str_mv Caruso, Vanni; Sheedharan, Smitha; Carlini, Valeria Paola; Jacobsson, Josefin A.; Haitina, Tatjana; et al.; mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts; Elsevier Science; Gene; 581; 2; 5-2016; 139-145
0378-1119
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111916001062
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2016.01.044
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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