Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
- Autores
- Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; Comai, Luca
- Año de publicación
- 2002
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation.
Fil: Madlung, Andreas. University of Washington; Estados Unidos
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Watson, Brian. University of Washington; Estados Unidos
Fil: Reynolds, Steve H.. University of Washington; Estados Unidos
Fil: Davison, Jerry. University of Washington; Estados Unidos
Fil: Comai, Luca. University of Washington; Estados Unidos - Materia
-
POLYPLOIDY
HYBRIDS
METHYLATION
ARABIDOPSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/195500
Ver los metadatos del registro completo
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spelling |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploidsMadlung, AndreasMasuelli, Ricardo WilliamsWatson, BrianReynolds, Steve H.Davison, JerryComai, LucaPOLYPLOIDYHYBRIDSMETHYLATIONARABIDOPSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation.Fil: Madlung, Andreas. University of Washington; Estados UnidosFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Watson, Brian. University of Washington; Estados UnidosFil: Reynolds, Steve H.. University of Washington; Estados UnidosFil: Davison, Jerry. University of Washington; Estados UnidosFil: Comai, Luca. University of Washington; Estados UnidosOxford University Press2002-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195500Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; et al.; Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids; Oxford University Press; Plant Physiology; 129; 2; 1-6-2002; 733-7460032-08891532-2548CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/129/2/733/6110301info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.003095info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:43Zoai:ri.conicet.gov.ar:11336/195500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:43.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
title |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
spellingShingle |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids Madlung, Andreas POLYPLOIDY HYBRIDS METHYLATION ARABIDOPSIS |
title_short |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
title_full |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
title_fullStr |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
title_full_unstemmed |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
title_sort |
Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids |
dc.creator.none.fl_str_mv |
Madlung, Andreas Masuelli, Ricardo Williams Watson, Brian Reynolds, Steve H. Davison, Jerry Comai, Luca |
author |
Madlung, Andreas |
author_facet |
Madlung, Andreas Masuelli, Ricardo Williams Watson, Brian Reynolds, Steve H. Davison, Jerry Comai, Luca |
author_role |
author |
author2 |
Masuelli, Ricardo Williams Watson, Brian Reynolds, Steve H. Davison, Jerry Comai, Luca |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
POLYPLOIDY HYBRIDS METHYLATION ARABIDOPSIS |
topic |
POLYPLOIDY HYBRIDS METHYLATION ARABIDOPSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation. Fil: Madlung, Andreas. University of Washington; Estados Unidos Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Watson, Brian. University of Washington; Estados Unidos Fil: Reynolds, Steve H.. University of Washington; Estados Unidos Fil: Davison, Jerry. University of Washington; Estados Unidos Fil: Comai, Luca. University of Washington; Estados Unidos |
description |
The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-06-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/195500 Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; et al.; Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids; Oxford University Press; Plant Physiology; 129; 2; 1-6-2002; 733-746 0032-0889 1532-2548 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/195500 |
identifier_str_mv |
Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; et al.; Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids; Oxford University Press; Plant Physiology; 129; 2; 1-6-2002; 733-746 0032-0889 1532-2548 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/129/2/733/6110301 info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.003095 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614436414816256 |
score |
13.070432 |