Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids

Autores
Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; Comai, Luca
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation.
Fil: Madlung, Andreas. University of Washington; Estados Unidos
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Watson, Brian. University of Washington; Estados Unidos
Fil: Reynolds, Steve H.. University of Washington; Estados Unidos
Fil: Davison, Jerry. University of Washington; Estados Unidos
Fil: Comai, Luca. University of Washington; Estados Unidos
Materia
POLYPLOIDY
HYBRIDS
METHYLATION
ARABIDOPSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195500

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spelling Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploidsMadlung, AndreasMasuelli, Ricardo WilliamsWatson, BrianReynolds, Steve H.Davison, JerryComai, LucaPOLYPLOIDYHYBRIDSMETHYLATIONARABIDOPSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation.Fil: Madlung, Andreas. University of Washington; Estados UnidosFil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Watson, Brian. University of Washington; Estados UnidosFil: Reynolds, Steve H.. University of Washington; Estados UnidosFil: Davison, Jerry. University of Washington; Estados UnidosFil: Comai, Luca. University of Washington; Estados UnidosOxford University Press2002-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195500Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; et al.; Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids; Oxford University Press; Plant Physiology; 129; 2; 1-6-2002; 733-7460032-08891532-2548CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/129/2/733/6110301info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.003095info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:43Zoai:ri.conicet.gov.ar:11336/195500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:43.663CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
title Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
spellingShingle Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
Madlung, Andreas
POLYPLOIDY
HYBRIDS
METHYLATION
ARABIDOPSIS
title_short Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
title_full Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
title_fullStr Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
title_full_unstemmed Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
title_sort Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids
dc.creator.none.fl_str_mv Madlung, Andreas
Masuelli, Ricardo Williams
Watson, Brian
Reynolds, Steve H.
Davison, Jerry
Comai, Luca
author Madlung, Andreas
author_facet Madlung, Andreas
Masuelli, Ricardo Williams
Watson, Brian
Reynolds, Steve H.
Davison, Jerry
Comai, Luca
author_role author
author2 Masuelli, Ricardo Williams
Watson, Brian
Reynolds, Steve H.
Davison, Jerry
Comai, Luca
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv POLYPLOIDY
HYBRIDS
METHYLATION
ARABIDOPSIS
topic POLYPLOIDY
HYBRIDS
METHYLATION
ARABIDOPSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation.
Fil: Madlung, Andreas. University of Washington; Estados Unidos
Fil: Masuelli, Ricardo Williams. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Watson, Brian. University of Washington; Estados Unidos
Fil: Reynolds, Steve H.. University of Washington; Estados Unidos
Fil: Davison, Jerry. University of Washington; Estados Unidos
Fil: Comai, Luca. University of Washington; Estados Unidos
description The joining of different genomes in allotetraploids played a major role in plant evolution, but the molecular implications of this event are poorly understood. In synthetic allotetraploids of Arabidopsis and Cardaminopsis arenosa, we previously demonstrated the occurrence of frequent gene silencing. To explore the involvement of epigenetic phenomena, we investigated the occurrence and effects of DNA methylation changes. Changes in DNA methylation patterns were more frequent in synthetic allotetraploids than in the parents. Treatment with 5-aza-2′-deoxycytidine, an inhibitor of DNA methyltransferase, resulted in the development of altered morphologies in the synthetic allotetraploids, but not in the parents. We profiled mRNAs in control and 5-aza-2′-deoxycytidine-treated parents and allotetraploids by amplified fragment length polymorphism-cDNA. We show that DNA demethylation induced and repressed two different transcriptomes. Our results are consistent with the hypothesis that synthetic allotetraploids have compromised mechanisms of epigenetic gene regulation.
publishDate 2002
dc.date.none.fl_str_mv 2002-06-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195500
Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; et al.; Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids; Oxford University Press; Plant Physiology; 129; 2; 1-6-2002; 733-746
0032-0889
1532-2548
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195500
identifier_str_mv Madlung, Andreas; Masuelli, Ricardo Williams; Watson, Brian; Reynolds, Steve H.; Davison, Jerry; et al.; Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids; Oxford University Press; Plant Physiology; 129; 2; 1-6-2002; 733-746
0032-0889
1532-2548
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/129/2/733/6110301
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.003095
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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