Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques

Autores
Colombo, Roxana; Fernandez Bidondo, Laura; Silvani, Vanesa Analia; Carbonetto, María Belén; Rascovan, N.; Bompadre, Maria Josefina; Pergola, Mariana; Cuenca, G.; Godeas, Alicia Margarita
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to assess the effects of agronomic practices on arbuscular mycorrhizal (AM) fungal community in soils from the Pampa Ondulada region (Argentina), and to compare conclusions reached when using pyrosequencing or morphological approach. AM fungal diversity was assessed in three agricultural exploitations located at the Pampa Ondulada region (Argentina) by 454 amplicon pyrosequencing and morphological (based on spore traits) approaches. Two kinds of soil managements are found in these sites (agronomic and non-agronomic). 188 Molecular Operational Taxonomic Units (MOTUs) and 29 morphological species of AM fungi were identified in total. Non effect of soil management on AM richness was detected. AM fungal communities were more diverse and equitable in absence of agronomic management. In contrast, results on beta diversity varied according to the methodology used. We concluded that agronomic management of soil has a negative effect on AM fungal community biodiversity in the Pampa Ondulada region. We also conclude that both methodologies complement each other in the study of AM fungal ecology. This study greatly improved the knowledge about AM fungi in South America where the molecular diversity of AM fungi was practically unknown.
Fil: Colombo, Roxana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez Bidondo, Laura. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina
Fil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Rascovan, N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Bompadre, Maria Josefina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pergola, Mariana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina
Fil: Cuenca, G.. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina
Materia
Glomeromycota
Agroecosystem
Biodiversity
Pyrosequencing Technology
Morphotyping Taxonomy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31485
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniquesColombo, RoxanaFernandez Bidondo, LauraSilvani, Vanesa AnaliaCarbonetto, María BelénRascovan, N.Bompadre, Maria JosefinaPergola, MarianaCuenca, G.Godeas, Alicia MargaritaGlomeromycotaAgroecosystemBiodiversityPyrosequencing TechnologyMorphotyping Taxonomyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to assess the effects of agronomic practices on arbuscular mycorrhizal (AM) fungal community in soils from the Pampa Ondulada region (Argentina), and to compare conclusions reached when using pyrosequencing or morphological approach. AM fungal diversity was assessed in three agricultural exploitations located at the Pampa Ondulada region (Argentina) by 454 amplicon pyrosequencing and morphological (based on spore traits) approaches. Two kinds of soil managements are found in these sites (agronomic and non-agronomic). 188 Molecular Operational Taxonomic Units (MOTUs) and 29 morphological species of AM fungi were identified in total. Non effect of soil management on AM richness was detected. AM fungal communities were more diverse and equitable in absence of agronomic management. In contrast, results on beta diversity varied according to the methodology used. We concluded that agronomic management of soil has a negative effect on AM fungal community biodiversity in the Pampa Ondulada region. We also conclude that both methodologies complement each other in the study of AM fungal ecology. This study greatly improved the knowledge about AM fungi in South America where the molecular diversity of AM fungi was practically unknown.Fil: Colombo, Roxana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez Bidondo, Laura. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; ArgentinaFil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Rascovan, N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Bompadre, Maria Josefina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pergola, Mariana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; ArgentinaFil: Cuenca, G.. Instituto Venezolano de Investigaciones Científicas; VenezuelaFil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; ArgentinaNational Research Council Canada-NRC Research Press2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31485Godeas, Alicia Margarita; Cuenca, G.; Pergola, Mariana; Bompadre, Maria Josefina; Rascovan, N.; Carbonetto, María Belén; et al.; Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 60; 12; 12-2014; 819-8270008-4166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2014-0364info:eu-repo/semantics/altIdentifier/doi/10.1139/cjm-2014-0364info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:31Zoai:ri.conicet.gov.ar:11336/31485instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:31.499CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
title Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
spellingShingle Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
Colombo, Roxana
Glomeromycota
Agroecosystem
Biodiversity
Pyrosequencing Technology
Morphotyping Taxonomy
title_short Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
title_full Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
title_fullStr Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
title_full_unstemmed Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
title_sort Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques
dc.creator.none.fl_str_mv Colombo, Roxana
Fernandez Bidondo, Laura
Silvani, Vanesa Analia
Carbonetto, María Belén
Rascovan, N.
Bompadre, Maria Josefina
Pergola, Mariana
Cuenca, G.
Godeas, Alicia Margarita
author Colombo, Roxana
author_facet Colombo, Roxana
Fernandez Bidondo, Laura
Silvani, Vanesa Analia
Carbonetto, María Belén
Rascovan, N.
Bompadre, Maria Josefina
Pergola, Mariana
Cuenca, G.
Godeas, Alicia Margarita
author_role author
author2 Fernandez Bidondo, Laura
Silvani, Vanesa Analia
Carbonetto, María Belén
Rascovan, N.
Bompadre, Maria Josefina
Pergola, Mariana
Cuenca, G.
Godeas, Alicia Margarita
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Glomeromycota
Agroecosystem
Biodiversity
Pyrosequencing Technology
Morphotyping Taxonomy
topic Glomeromycota
Agroecosystem
Biodiversity
Pyrosequencing Technology
Morphotyping Taxonomy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to assess the effects of agronomic practices on arbuscular mycorrhizal (AM) fungal community in soils from the Pampa Ondulada region (Argentina), and to compare conclusions reached when using pyrosequencing or morphological approach. AM fungal diversity was assessed in three agricultural exploitations located at the Pampa Ondulada region (Argentina) by 454 amplicon pyrosequencing and morphological (based on spore traits) approaches. Two kinds of soil managements are found in these sites (agronomic and non-agronomic). 188 Molecular Operational Taxonomic Units (MOTUs) and 29 morphological species of AM fungi were identified in total. Non effect of soil management on AM richness was detected. AM fungal communities were more diverse and equitable in absence of agronomic management. In contrast, results on beta diversity varied according to the methodology used. We concluded that agronomic management of soil has a negative effect on AM fungal community biodiversity in the Pampa Ondulada region. We also conclude that both methodologies complement each other in the study of AM fungal ecology. This study greatly improved the knowledge about AM fungi in South America where the molecular diversity of AM fungi was practically unknown.
Fil: Colombo, Roxana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez Bidondo, Laura. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvani, Vanesa Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina
Fil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Rascovan, N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Bompadre, Maria Josefina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pergola, Mariana. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina
Fil: Cuenca, G.. Instituto Venezolano de Investigaciones Científicas; Venezuela
Fil: Godeas, Alicia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Microbiología del Suelo; Argentina
description The aim of this study was to assess the effects of agronomic practices on arbuscular mycorrhizal (AM) fungal community in soils from the Pampa Ondulada region (Argentina), and to compare conclusions reached when using pyrosequencing or morphological approach. AM fungal diversity was assessed in three agricultural exploitations located at the Pampa Ondulada region (Argentina) by 454 amplicon pyrosequencing and morphological (based on spore traits) approaches. Two kinds of soil managements are found in these sites (agronomic and non-agronomic). 188 Molecular Operational Taxonomic Units (MOTUs) and 29 morphological species of AM fungi were identified in total. Non effect of soil management on AM richness was detected. AM fungal communities were more diverse and equitable in absence of agronomic management. In contrast, results on beta diversity varied according to the methodology used. We concluded that agronomic management of soil has a negative effect on AM fungal community biodiversity in the Pampa Ondulada region. We also conclude that both methodologies complement each other in the study of AM fungal ecology. This study greatly improved the knowledge about AM fungi in South America where the molecular diversity of AM fungi was practically unknown.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31485
Godeas, Alicia Margarita; Cuenca, G.; Pergola, Mariana; Bompadre, Maria Josefina; Rascovan, N.; Carbonetto, María Belén; et al.; Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 60; 12; 12-2014; 819-827
0008-4166
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31485
identifier_str_mv Godeas, Alicia Margarita; Cuenca, G.; Pergola, Mariana; Bompadre, Maria Josefina; Rascovan, N.; Carbonetto, María Belén; et al.; Diversity of arbuscular mycorrhizal fungi in soil from the Pampa Ondulada, Argentina, assesed by pyrosequencing and traditional techniques; National Research Council Canada-NRC Research Press; Canadian Journal Of Microbiology; 60; 12; 12-2014; 819-827
0008-4166
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2014-0364
info:eu-repo/semantics/altIdentifier/doi/10.1139/cjm-2014-0364
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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