Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration

Autores
Martín González, Natalia; Guérin Darvas, Sofía M.; Durana, Aritz; Marti, Gerardo Anibal; Guérin, Diego M. A.; de Pablo, Pedro J.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Even though viruses evolve mainly in liquid milieu, their horizontal transmission routesoften include episodes of dry environment. Along their life cycle, some insect viruses, suchas viruses from the Dicistroviridae family, withstand dehydrated conditions with presentlyunknown consequences to their structural stability. Here, we use atomic force microscopy tomonitor the structural changes of viral particles of Triatoma virus (TrV) after desiccation. Ourresults demonstrate that TrV capsids preserve their genome inside, conserving their heightafter exposure to dehydrating conditions, which is in stark contrast with other viruses thatexpel their genome when desiccated. Moreover, empty capsids (without genome) resultedin collapsed particles after desiccation. We also explored the role of structural ions in thedehydration process of the virions (capsid containing genome) by chelating the accessiblecations from the external solvent milieu. We observed that ion suppression helps to keep thevirus height upon desiccation. Our results show that under drying conditions, the genomeof TrV prevents the capsid from collapsing during dehydration, while the structural ions areresponsible for promoting solvent exchange through the virion wall.
Fil: Martín González, Natalia. Universidad Autónoma de Madrid; España
Fil: Guérin Darvas, Sofía M.. Universidad del País Vasco; España
Fil: Durana, Aritz. Universidad del País Vasco; España
Fil: Marti, Gerardo Anibal. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Guérin, Diego M. A.. Universidad del País Vasco; España
Fil: de Pablo, Pedro J.. Universidad Autónoma de Madrid; España
Materia
AFM
ATOMIC FORCE MICROSCOPY
HYDROPHOBIC GATE
ICOSAHEDRAL NON-ENVELOPED VIRUSES
TRIATOMA VIRUS
VIRUS DEHYDRATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/86814
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/86814
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydrationMartín González, NataliaGuérin Darvas, Sofía M.Durana, AritzMarti, Gerardo AnibalGuérin, Diego M. A.de Pablo, Pedro J.AFMATOMIC FORCE MICROSCOPYHYDROPHOBIC GATEICOSAHEDRAL NON-ENVELOPED VIRUSESTRIATOMA VIRUSVIRUS DEHYDRATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Even though viruses evolve mainly in liquid milieu, their horizontal transmission routesoften include episodes of dry environment. Along their life cycle, some insect viruses, suchas viruses from the Dicistroviridae family, withstand dehydrated conditions with presentlyunknown consequences to their structural stability. Here, we use atomic force microscopy tomonitor the structural changes of viral particles of Triatoma virus (TrV) after desiccation. Ourresults demonstrate that TrV capsids preserve their genome inside, conserving their heightafter exposure to dehydrating conditions, which is in stark contrast with other viruses thatexpel their genome when desiccated. Moreover, empty capsids (without genome) resultedin collapsed particles after desiccation. We also explored the role of structural ions in thedehydration process of the virions (capsid containing genome) by chelating the accessiblecations from the external solvent milieu. We observed that ion suppression helps to keep thevirus height upon desiccation. Our results show that under drying conditions, the genomeof TrV prevents the capsid from collapsing during dehydration, while the structural ions areresponsible for promoting solvent exchange through the virion wall.Fil: Martín González, Natalia. Universidad Autónoma de Madrid; EspañaFil: Guérin Darvas, Sofía M.. Universidad del País Vasco; EspañaFil: Durana, Aritz. Universidad del País Vasco; EspañaFil: Marti, Gerardo Anibal. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Guérin, Diego M. A.. Universidad del País Vasco; EspañaFil: de Pablo, Pedro J.. Universidad Autónoma de Madrid; EspañaIOP Publishing2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/86814Martín González, Natalia; Guérin Darvas, Sofía M.; Durana, Aritz; Marti, Gerardo Anibal; Guérin, Diego M. A.; et al.; Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration; IOP Publishing; Journal of Physics: Condensed Matter; 30; 10; 6-2018; 1-90953-8984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1088/1361-648X/aaa944info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1361-648X/aaa944info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:21:48Zoai:ri.conicet.gov.ar:11336/86814instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:21:48.448CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
title Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
spellingShingle Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
Martín González, Natalia
AFM
ATOMIC FORCE MICROSCOPY
HYDROPHOBIC GATE
ICOSAHEDRAL NON-ENVELOPED VIRUSES
TRIATOMA VIRUS
VIRUS DEHYDRATION
title_short Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
title_full Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
title_fullStr Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
title_full_unstemmed Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
title_sort Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration
dc.creator.none.fl_str_mv Martín González, Natalia
Guérin Darvas, Sofía M.
Durana, Aritz
Marti, Gerardo Anibal
Guérin, Diego M. A.
de Pablo, Pedro J.
author Martín González, Natalia
author_facet Martín González, Natalia
Guérin Darvas, Sofía M.
Durana, Aritz
Marti, Gerardo Anibal
Guérin, Diego M. A.
de Pablo, Pedro J.
author_role author
author2 Guérin Darvas, Sofía M.
Durana, Aritz
Marti, Gerardo Anibal
Guérin, Diego M. A.
de Pablo, Pedro J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AFM
ATOMIC FORCE MICROSCOPY
HYDROPHOBIC GATE
ICOSAHEDRAL NON-ENVELOPED VIRUSES
TRIATOMA VIRUS
VIRUS DEHYDRATION
topic AFM
ATOMIC FORCE MICROSCOPY
HYDROPHOBIC GATE
ICOSAHEDRAL NON-ENVELOPED VIRUSES
TRIATOMA VIRUS
VIRUS DEHYDRATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Even though viruses evolve mainly in liquid milieu, their horizontal transmission routesoften include episodes of dry environment. Along their life cycle, some insect viruses, suchas viruses from the Dicistroviridae family, withstand dehydrated conditions with presentlyunknown consequences to their structural stability. Here, we use atomic force microscopy tomonitor the structural changes of viral particles of Triatoma virus (TrV) after desiccation. Ourresults demonstrate that TrV capsids preserve their genome inside, conserving their heightafter exposure to dehydrating conditions, which is in stark contrast with other viruses thatexpel their genome when desiccated. Moreover, empty capsids (without genome) resultedin collapsed particles after desiccation. We also explored the role of structural ions in thedehydration process of the virions (capsid containing genome) by chelating the accessiblecations from the external solvent milieu. We observed that ion suppression helps to keep thevirus height upon desiccation. Our results show that under drying conditions, the genomeof TrV prevents the capsid from collapsing during dehydration, while the structural ions areresponsible for promoting solvent exchange through the virion wall.
Fil: Martín González, Natalia. Universidad Autónoma de Madrid; España
Fil: Guérin Darvas, Sofía M.. Universidad del País Vasco; España
Fil: Durana, Aritz. Universidad del País Vasco; España
Fil: Marti, Gerardo Anibal. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Guérin, Diego M. A.. Universidad del País Vasco; España
Fil: de Pablo, Pedro J.. Universidad Autónoma de Madrid; España
description Even though viruses evolve mainly in liquid milieu, their horizontal transmission routesoften include episodes of dry environment. Along their life cycle, some insect viruses, suchas viruses from the Dicistroviridae family, withstand dehydrated conditions with presentlyunknown consequences to their structural stability. Here, we use atomic force microscopy tomonitor the structural changes of viral particles of Triatoma virus (TrV) after desiccation. Ourresults demonstrate that TrV capsids preserve their genome inside, conserving their heightafter exposure to dehydrating conditions, which is in stark contrast with other viruses thatexpel their genome when desiccated. Moreover, empty capsids (without genome) resultedin collapsed particles after desiccation. We also explored the role of structural ions in thedehydration process of the virions (capsid containing genome) by chelating the accessiblecations from the external solvent milieu. We observed that ion suppression helps to keep thevirus height upon desiccation. Our results show that under drying conditions, the genomeof TrV prevents the capsid from collapsing during dehydration, while the structural ions areresponsible for promoting solvent exchange through the virion wall.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/86814
Martín González, Natalia; Guérin Darvas, Sofía M.; Durana, Aritz; Marti, Gerardo Anibal; Guérin, Diego M. A.; et al.; Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration; IOP Publishing; Journal of Physics: Condensed Matter; 30; 10; 6-2018; 1-9
0953-8984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/86814
identifier_str_mv Martín González, Natalia; Guérin Darvas, Sofía M.; Durana, Aritz; Marti, Gerardo Anibal; Guérin, Diego M. A.; et al.; Exploring the role of genome and structural ions in preventing viral capsid collapse during dehydration; IOP Publishing; Journal of Physics: Condensed Matter; 30; 10; 6-2018; 1-9
0953-8984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1088/1361-648X/aaa944
info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1361-648X/aaa944
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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