Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
- Autores
- Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; Nava, Santiago; Sebastian, Patrick Stephan
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far.
Fil: Maas, Lara M. I.. Universitat Leipzig; Alemania
Fil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Herrmann, Verónica. No especifíca;
Fil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Obiegala, Anna. Universitat Leipzig; Alemania
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina - Materia
-
ANAPLASMA
RICKETTSIA
TICKS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/260487
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Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of ArgentinaMaas, Lara M. I.Winter, MarinaHerrmann, VerónicaAbate, Sergio DamianObiegala, AnnaNava, SantiagoSebastian, Patrick StephanANAPLASMARICKETTSIATICKShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far.Fil: Maas, Lara M. I.. Universitat Leipzig; AlemaniaFil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Herrmann, Verónica. No especifíca;Fil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Obiegala, Anna. Universitat Leipzig; AlemaniaFil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaCambridge University Press2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260487Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; et al.; Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina; Cambridge University Press; Parasitology; 151; 8; 10-2024; 848-8520031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S0031182024000933/type/journal_articleinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182024000933info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:59Zoai:ri.conicet.gov.ar:11336/260487instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:59.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
title |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
spellingShingle |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina Maas, Lara M. I. ANAPLASMA RICKETTSIA TICKS |
title_short |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
title_full |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
title_fullStr |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
title_full_unstemmed |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
title_sort |
Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina |
dc.creator.none.fl_str_mv |
Maas, Lara M. I. Winter, Marina Herrmann, Verónica Abate, Sergio Damian Obiegala, Anna Nava, Santiago Sebastian, Patrick Stephan |
author |
Maas, Lara M. I. |
author_facet |
Maas, Lara M. I. Winter, Marina Herrmann, Verónica Abate, Sergio Damian Obiegala, Anna Nava, Santiago Sebastian, Patrick Stephan |
author_role |
author |
author2 |
Winter, Marina Herrmann, Verónica Abate, Sergio Damian Obiegala, Anna Nava, Santiago Sebastian, Patrick Stephan |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
ANAPLASMA RICKETTSIA TICKS |
topic |
ANAPLASMA RICKETTSIA TICKS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far. Fil: Maas, Lara M. I.. Universitat Leipzig; Alemania Fil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina Fil: Herrmann, Verónica. No especifíca; Fil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina Fil: Obiegala, Anna. Universitat Leipzig; Alemania Fil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina |
description |
The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/260487 Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; et al.; Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina; Cambridge University Press; Parasitology; 151; 8; 10-2024; 848-852 0031-1820 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/260487 |
identifier_str_mv |
Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; et al.; Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina; Cambridge University Press; Parasitology; 151; 8; 10-2024; 848-852 0031-1820 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S0031182024000933/type/journal_article info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182024000933 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge University Press |
publisher.none.fl_str_mv |
Cambridge University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613415892418560 |
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13.070432 |