Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina

Autores
Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; Nava, Santiago; Sebastian, Patrick Stephan
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far.
Fil: Maas, Lara M. I.. Universitat Leipzig; Alemania
Fil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Herrmann, Verónica. No especifíca;
Fil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Obiegala, Anna. Universitat Leipzig; Alemania
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Materia
ANAPLASMA
RICKETTSIA
TICKS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260487
Acceder
id CONICETDig_d79f44e206af5eba1e745cbecd99d5ac
oai_identifier_str oai:ri.conicet.gov.ar:11336/260487
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of ArgentinaMaas, Lara M. I.Winter, MarinaHerrmann, VerónicaAbate, Sergio DamianObiegala, AnnaNava, SantiagoSebastian, Patrick StephanANAPLASMARICKETTSIATICKShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far.Fil: Maas, Lara M. I.. Universitat Leipzig; AlemaniaFil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Herrmann, Verónica. No especifíca;Fil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Obiegala, Anna. Universitat Leipzig; AlemaniaFil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaCambridge University Press2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260487Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; et al.; Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina; Cambridge University Press; Parasitology; 151; 8; 10-2024; 848-8520031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S0031182024000933/type/journal_articleinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182024000933info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:13:53Zoai:ri.conicet.gov.ar:11336/260487instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:13:53.416CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
title Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
spellingShingle Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
Maas, Lara M. I.
ANAPLASMA
RICKETTSIA
TICKS
title_short Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
title_full Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
title_fullStr Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
title_full_unstemmed Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
title_sort Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina
dc.creator.none.fl_str_mv Maas, Lara M. I.
Winter, Marina
Herrmann, Verónica
Abate, Sergio Damian
Obiegala, Anna
Nava, Santiago
Sebastian, Patrick Stephan
author Maas, Lara M. I.
author_facet Maas, Lara M. I.
Winter, Marina
Herrmann, Verónica
Abate, Sergio Damian
Obiegala, Anna
Nava, Santiago
Sebastian, Patrick Stephan
author_role author
author2 Winter, Marina
Herrmann, Verónica
Abate, Sergio Damian
Obiegala, Anna
Nava, Santiago
Sebastian, Patrick Stephan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANAPLASMA
RICKETTSIA
TICKS
topic ANAPLASMA
RICKETTSIA
TICKS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far.
Fil: Maas, Lara M. I.. Universitat Leipzig; Alemania
Fil: Winter, Marina. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Herrmann, Verónica. No especifíca;
Fil: Abate, Sergio Damian. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Obiegala, Anna. Universitat Leipzig; Alemania
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
description The aim of this study was to examine the presence of tick-borne rickettsial bacteria inRhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian regionof Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examinedfor the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified tospecies level. DNA was extracted and analysed by conventional PCR assays for the presence oftick-borne bacteria belonging to the genera Anaplasma, Ehrlichia and Rickettsia. Thirty-threetick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceaebacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences weregenerated and belonging to Anaplasma platys, the causative agent of canine cyclic thrombocytopenia.Two tick samples were positive in Rickettsia specific PCR assays and were identifiedby phylogenetic analysis as Rickettsia massiliae, a member of the spotted fever group rickettsiae.The results of this study demonstrate the molecular detection of 2 rickettsial bacteria inR. sanguineus s.s. in a region of Argentina where no data were available so far.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260487
Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; et al.; Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina; Cambridge University Press; Parasitology; 151; 8; 10-2024; 848-852
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260487
identifier_str_mv Maas, Lara M. I.; Winter, Marina; Herrmann, Verónica; Abate, Sergio Damian; Obiegala, Anna; et al.; Anaplasma platys and Rickettsia massiliae in Rhipicephalus sanguineus sensu stricto ticks collected on dogs in the Patagonian region of Argentina; Cambridge University Press; Parasitology; 151; 8; 10-2024; 848-852
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S0031182024000933/type/journal_article
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182024000933
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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