Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation

Autores
Morado, Sergio Adrián; Cetica, Pablo Daniel; Beconi, Martha Teresa; Thompson, J. G.; Dalvit, Gabriel Carlos
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen–thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7–24 h from activation) by 20 ,70 -dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P!0.05 by ANOVA). In sperm-activated oocytes, an increase was observed between 15 and 19 h (P!0.05). Conversely, in parthenogenetically activated oocytes, we observed a decrease at 9 h (P!0.05). In sperm-activated oocytes, ROS fluctuated throughout the 24 h, presenting peaks around 7, 19, and 24 h (P!0.05), while in parthenogenetic activation, peaks were detected at 7, 11, and 17 h (P!0.05). In the present work, we found clear distinctive metabolic patterns between normal and parthenogenetic zygotes. Oxidative activity and ROS production are an integral part of bovine zygote behavior, and defining a temporal pattern of change may be linked with developmental competence.
Fil: Morado, Sergio Adrián. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beconi, Martha Teresa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Thompson, J. G.. University of Adelaide; Australia
Fil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Embryo Development
In Vitro Fertilization
Oocyte Development
Parthenogenetics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26264
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26264
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activationMorado, Sergio AdriánCetica, Pablo DanielBeconi, Martha TeresaThompson, J. G.Dalvit, Gabriel CarlosEmbryo DevelopmentIn Vitro FertilizationOocyte DevelopmentParthenogeneticsThe knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen–thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7–24 h from activation) by 20 ,70 -dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P!0.05 by ANOVA). In sperm-activated oocytes, an increase was observed between 15 and 19 h (P!0.05). Conversely, in parthenogenetically activated oocytes, we observed a decrease at 9 h (P!0.05). In sperm-activated oocytes, ROS fluctuated throughout the 24 h, presenting peaks around 7, 19, and 24 h (P!0.05), while in parthenogenetic activation, peaks were detected at 7, 11, and 17 h (P!0.05). In the present work, we found clear distinctive metabolic patterns between normal and parthenogenetic zygotes. Oxidative activity and ROS production are an integral part of bovine zygote behavior, and defining a temporal pattern of change may be linked with developmental competence.Fil: Morado, Sergio Adrián. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Beconi, Martha Teresa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Thompson, J. G.. University of Adelaide; AustraliaFil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBioScientifica2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26264Morado, Sergio Adrián; Cetica, Pablo Daniel; Beconi, Martha Teresa; Thompson, J. G.; Dalvit, Gabriel Carlos; Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation; BioScientifica; Reproduction; 145; 5; 5-2013; 471-4781470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-13-0017info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/145/5/471info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:26Zoai:ri.conicet.gov.ar:11336/26264instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:27.17CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
title Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
spellingShingle Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
Morado, Sergio Adrián
Embryo Development
In Vitro Fertilization
Oocyte Development
Parthenogenetics
title_short Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
title_full Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
title_fullStr Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
title_full_unstemmed Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
title_sort Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation
dc.creator.none.fl_str_mv Morado, Sergio Adrián
Cetica, Pablo Daniel
Beconi, Martha Teresa
Thompson, J. G.
Dalvit, Gabriel Carlos
author Morado, Sergio Adrián
author_facet Morado, Sergio Adrián
Cetica, Pablo Daniel
Beconi, Martha Teresa
Thompson, J. G.
Dalvit, Gabriel Carlos
author_role author
author2 Cetica, Pablo Daniel
Beconi, Martha Teresa
Thompson, J. G.
Dalvit, Gabriel Carlos
author2_role author
author
author
author
dc.subject.none.fl_str_mv Embryo Development
In Vitro Fertilization
Oocyte Development
Parthenogenetics
topic Embryo Development
In Vitro Fertilization
Oocyte Development
Parthenogenetics
dc.description.none.fl_txt_mv The knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen–thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7–24 h from activation) by 20 ,70 -dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P!0.05 by ANOVA). In sperm-activated oocytes, an increase was observed between 15 and 19 h (P!0.05). Conversely, in parthenogenetically activated oocytes, we observed a decrease at 9 h (P!0.05). In sperm-activated oocytes, ROS fluctuated throughout the 24 h, presenting peaks around 7, 19, and 24 h (P!0.05), while in parthenogenetic activation, peaks were detected at 7, 11, and 17 h (P!0.05). In the present work, we found clear distinctive metabolic patterns between normal and parthenogenetic zygotes. Oxidative activity and ROS production are an integral part of bovine zygote behavior, and defining a temporal pattern of change may be linked with developmental competence.
Fil: Morado, Sergio Adrián. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cetica, Pablo Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Beconi, Martha Teresa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Thompson, J. G.. University of Adelaide; Australia
Fil: Dalvit, Gabriel Carlos. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen–thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7–24 h from activation) by 20 ,70 -dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P!0.05 by ANOVA). In sperm-activated oocytes, an increase was observed between 15 and 19 h (P!0.05). Conversely, in parthenogenetically activated oocytes, we observed a decrease at 9 h (P!0.05). In sperm-activated oocytes, ROS fluctuated throughout the 24 h, presenting peaks around 7, 19, and 24 h (P!0.05), while in parthenogenetic activation, peaks were detected at 7, 11, and 17 h (P!0.05). In the present work, we found clear distinctive metabolic patterns between normal and parthenogenetic zygotes. Oxidative activity and ROS production are an integral part of bovine zygote behavior, and defining a temporal pattern of change may be linked with developmental competence.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26264
Morado, Sergio Adrián; Cetica, Pablo Daniel; Beconi, Martha Teresa; Thompson, J. G.; Dalvit, Gabriel Carlos; Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation; BioScientifica; Reproduction; 145; 5; 5-2013; 471-478
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26264
identifier_str_mv Morado, Sergio Adrián; Cetica, Pablo Daniel; Beconi, Martha Teresa; Thompson, J. G.; Dalvit, Gabriel Carlos; Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation; BioScientifica; Reproduction; 145; 5; 5-2013; 471-478
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-13-0017
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/145/5/471
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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