Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation

Autores
Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndrome
Fil: Artur de la Villarmois, Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Constantin, Maria Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Perez, Maria Fernanda. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Materia
Nitric Oxide Sintase
Hippocampus
Diazepam dependence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89634
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Diazepam withdrawal expression is related to hippocampal NOS-1 upregulationArtur de la Villarmois, EmilceConstantin, Maria FlorenciaBregonzio Diaz, ClaudiaPerez, Maria FernandaNitric Oxide SintaseHippocampusDiazepam dependencehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndromeFil: Artur de la Villarmois, Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Constantin, Maria Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Perez, Maria Fernanda. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaHeighten Science Publications2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89634Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda; Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation; Heighten Science Publications; Archives of Pharmacy and Pharmaceutical Sciences; 2; 1; 1-2018; 001-0092576-9537CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/https://www.heighpubs.org/hps/pdf/apps-aid1006.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.29328/journal.hps.1001006info:eu-repo/semantics/altIdentifier/url/https://www.heighpubs.org/hps/apps-aid1006.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:08:13Zoai:ri.conicet.gov.ar:11336/89634instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:08:13.531CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
title Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
spellingShingle Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
Artur de la Villarmois, Emilce
Nitric Oxide Sintase
Hippocampus
Diazepam dependence
title_short Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
title_full Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
title_fullStr Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
title_full_unstemmed Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
title_sort Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
dc.creator.none.fl_str_mv Artur de la Villarmois, Emilce
Constantin, Maria Florencia
Bregonzio Diaz, Claudia
Perez, Maria Fernanda
author Artur de la Villarmois, Emilce
author_facet Artur de la Villarmois, Emilce
Constantin, Maria Florencia
Bregonzio Diaz, Claudia
Perez, Maria Fernanda
author_role author
author2 Constantin, Maria Florencia
Bregonzio Diaz, Claudia
Perez, Maria Fernanda
author2_role author
author
author
dc.subject.none.fl_str_mv Nitric Oxide Sintase
Hippocampus
Diazepam dependence
topic Nitric Oxide Sintase
Hippocampus
Diazepam dependence
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndrome
Fil: Artur de la Villarmois, Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Constantin, Maria Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Perez, Maria Fernanda. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
description Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndrome
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89634
Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda; Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation; Heighten Science Publications; Archives of Pharmacy and Pharmaceutical Sciences; 2; 1; 1-2018; 001-009
2576-9537
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89634
identifier_str_mv Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda; Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation; Heighten Science Publications; Archives of Pharmacy and Pharmaceutical Sciences; 2; 1; 1-2018; 001-009
2576-9537
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/url/https://www.heighpubs.org/hps/pdf/apps-aid1006.pdf
info:eu-repo/semantics/altIdentifier/doi/10.29328/journal.hps.1001006
info:eu-repo/semantics/altIdentifier/url/https://www.heighpubs.org/hps/apps-aid1006.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Heighten Science Publications
publisher.none.fl_str_mv Heighten Science Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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