Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation
- Autores
- Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndrome
Fil: Artur de la Villarmois, Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Constantin, Maria Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Perez, Maria Fernanda. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina - Materia
-
Nitric Oxide Sintase
Hippocampus
Diazepam dependence - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/89634
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Diazepam withdrawal expression is related to hippocampal NOS-1 upregulationArtur de la Villarmois, EmilceConstantin, Maria FlorenciaBregonzio Diaz, ClaudiaPerez, Maria FernandaNitric Oxide SintaseHippocampusDiazepam dependencehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndromeFil: Artur de la Villarmois, Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Constantin, Maria Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Perez, Maria Fernanda. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaHeighten Science Publications2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89634Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda; Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation; Heighten Science Publications; Archives of Pharmacy and Pharmaceutical Sciences; 2; 1; 1-2018; 001-0092576-9537CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/https://www.heighpubs.org/hps/pdf/apps-aid1006.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.29328/journal.hps.1001006info:eu-repo/semantics/altIdentifier/url/https://www.heighpubs.org/hps/apps-aid1006.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:46Zoai:ri.conicet.gov.ar:11336/89634instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:46.53CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
title |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
spellingShingle |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation Artur de la Villarmois, Emilce Nitric Oxide Sintase Hippocampus Diazepam dependence |
title_short |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
title_full |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
title_fullStr |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
title_full_unstemmed |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
title_sort |
Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation |
dc.creator.none.fl_str_mv |
Artur de la Villarmois, Emilce Constantin, Maria Florencia Bregonzio Diaz, Claudia Perez, Maria Fernanda |
author |
Artur de la Villarmois, Emilce |
author_facet |
Artur de la Villarmois, Emilce Constantin, Maria Florencia Bregonzio Diaz, Claudia Perez, Maria Fernanda |
author_role |
author |
author2 |
Constantin, Maria Florencia Bregonzio Diaz, Claudia Perez, Maria Fernanda |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Nitric Oxide Sintase Hippocampus Diazepam dependence |
topic |
Nitric Oxide Sintase Hippocampus Diazepam dependence |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndrome Fil: Artur de la Villarmois, Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina Fil: Constantin, Maria Florencia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina Fil: Perez, Maria Fernanda. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina |
description |
Benzodiazepines are usually prescribed for anxiety and sleep disorders in a long-term fashion that may cause drug dependence. Discontinuation after prolonged administration may lead to withdrawal expression, being anxiety the most predominant sign. It has been described that a context-dependent associative learning process underlies diazepam dependence. Nitric oxide is a crucial player in learning and memory processes, hippocampal transmission, as well as in benzodiazepines withdrawal. Considering that previous results from our laboratory showed an increase in hippocampal functional plasticity only in diazepam dependent rats, the aim of the present investigation is to determine whether diazepam dependence could alter neuronal nitric oxide synthase enzyme (NOS-1) expression within the hippocampus, by using western blot.Results: chronic diazepam-treated animals that developed dependence showed increase in NOS-1 expression in dorsal, but not in ventral hippocampus, while no-dependent or control animals presented similar NOS-1 protein levels.Conclusion: withdrawal from long-term diazepam exposure could be associated to increased nitric oxide neurotransmission within dorsal hippocampus induced by NOS-1 over-expression. This mechanism could underlie the improved hippocampal synaptic transmission previously observed in diazepam withdrawn animals. Confirmatory experiments need to be addressed to determine the mechanisms by which nitric oxide participates in benzodiazepines withdrawal in order find new molecular targets to develop pharmacological tools to prevent the withdrawal syndrome |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/89634 Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda; Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation; Heighten Science Publications; Archives of Pharmacy and Pharmaceutical Sciences; 2; 1; 1-2018; 001-009 2576-9537 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/89634 |
identifier_str_mv |
Artur de la Villarmois, Emilce; Constantin, Maria Florencia; Bregonzio Diaz, Claudia; Perez, Maria Fernanda; Diazepam withdrawal expression is related to hippocampal NOS-1 upregulation; Heighten Science Publications; Archives of Pharmacy and Pharmaceutical Sciences; 2; 1; 1-2018; 001-009 2576-9537 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/reference/url/https://www.heighpubs.org/hps/pdf/apps-aid1006.pdf info:eu-repo/semantics/altIdentifier/doi/10.29328/journal.hps.1001006 info:eu-repo/semantics/altIdentifier/url/https://www.heighpubs.org/hps/apps-aid1006.php |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Heighten Science Publications |
publisher.none.fl_str_mv |
Heighten Science Publications |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613259016011776 |
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13.070432 |