Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium

Autores
Raices, Marilina; Jungblut, Lucas David; Paz, Dante Agustin; Pozzi, Andrea Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vertebrates the most notable feature of the olfactory epithelium (OE) is its ability to continually replace cell types, including mature sensory neurons. In amphibians, the neuronal turnover is observed in adult animals and during larval development also. Today, one of the most used techniques for studies of cell proliferation and differentiation is the 5-bromo-2-deoxyuridine (BrdU) uptake, a synthetic analogue of thymidine that is incorporated into cells during DNA replication. Despite this, and because of its toxicity, some authors have questioned its use for the above mentioned purposes. This work analyses BrdU incorporation technique for cell proliferation and differentiation studies within the OE of amphibian larvae. The methodology used has been validated in Xenopus laevis larvae for studies related to inner ear formation. However, we observe a large number of adverse effects on cellular components of the OE, such as an increase in apoptosis, alterations in the expression pattern of different cell markers, as well as defects in the foraging behavior in animals that were exposed to BrdU. We conclude that even though BrdU can be successfully used as a proliferation cellular marker in the OE, It should be very careful when BrdU is used for studying cell lineage.
Fil: Raices, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Jungblut, Lucas David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Pozzi, Andrea Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Materia
AMPHIBIAN
OLFACTORY SYSTEM
BRDU
NEUROTOXICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182686
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory EpitheliumRaices, MarilinaJungblut, Lucas DavidPaz, Dante AgustinPozzi, Andrea GabrielaAMPHIBIANOLFACTORY SYSTEMBRDUNEUROTOXICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In vertebrates the most notable feature of the olfactory epithelium (OE) is its ability to continually replace cell types, including mature sensory neurons. In amphibians, the neuronal turnover is observed in adult animals and during larval development also. Today, one of the most used techniques for studies of cell proliferation and differentiation is the 5-bromo-2-deoxyuridine (BrdU) uptake, a synthetic analogue of thymidine that is incorporated into cells during DNA replication. Despite this, and because of its toxicity, some authors have questioned its use for the above mentioned purposes. This work analyses BrdU incorporation technique for cell proliferation and differentiation studies within the OE of amphibian larvae. The methodology used has been validated in Xenopus laevis larvae for studies related to inner ear formation. However, we observe a large number of adverse effects on cellular components of the OE, such as an increase in apoptosis, alterations in the expression pattern of different cell markers, as well as defects in the foraging behavior in animals that were exposed to BrdU. We conclude that even though BrdU can be successfully used as a proliferation cellular marker in the OE, It should be very careful when BrdU is used for studying cell lineage.Fil: Raices, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Jungblut, Lucas David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Pozzi, Andrea Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaMedCrave2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182686Raices, Marilina; Jungblut, Lucas David; Paz, Dante Agustin; Pozzi, Andrea Gabriela; Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium; MedCrave; MOJ Anatomy & Physiology; 1; 4; 11-2015; 1-92471-139XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://medcraveonline.com/MOJAP/MOJAP-01-00023.phpinfo:eu-repo/semantics/altIdentifier/doi/10.15406/mojap.2015.01.00023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:28Zoai:ri.conicet.gov.ar:11336/182686instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:28.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
title Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
spellingShingle Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
Raices, Marilina
AMPHIBIAN
OLFACTORY SYSTEM
BRDU
NEUROTOXICITY
title_short Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
title_full Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
title_fullStr Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
title_full_unstemmed Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
title_sort Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium
dc.creator.none.fl_str_mv Raices, Marilina
Jungblut, Lucas David
Paz, Dante Agustin
Pozzi, Andrea Gabriela
author Raices, Marilina
author_facet Raices, Marilina
Jungblut, Lucas David
Paz, Dante Agustin
Pozzi, Andrea Gabriela
author_role author
author2 Jungblut, Lucas David
Paz, Dante Agustin
Pozzi, Andrea Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv AMPHIBIAN
OLFACTORY SYSTEM
BRDU
NEUROTOXICITY
topic AMPHIBIAN
OLFACTORY SYSTEM
BRDU
NEUROTOXICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In vertebrates the most notable feature of the olfactory epithelium (OE) is its ability to continually replace cell types, including mature sensory neurons. In amphibians, the neuronal turnover is observed in adult animals and during larval development also. Today, one of the most used techniques for studies of cell proliferation and differentiation is the 5-bromo-2-deoxyuridine (BrdU) uptake, a synthetic analogue of thymidine that is incorporated into cells during DNA replication. Despite this, and because of its toxicity, some authors have questioned its use for the above mentioned purposes. This work analyses BrdU incorporation technique for cell proliferation and differentiation studies within the OE of amphibian larvae. The methodology used has been validated in Xenopus laevis larvae for studies related to inner ear formation. However, we observe a large number of adverse effects on cellular components of the OE, such as an increase in apoptosis, alterations in the expression pattern of different cell markers, as well as defects in the foraging behavior in animals that were exposed to BrdU. We conclude that even though BrdU can be successfully used as a proliferation cellular marker in the OE, It should be very careful when BrdU is used for studying cell lineage.
Fil: Raices, Marilina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Jungblut, Lucas David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Paz, Dante Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Pozzi, Andrea Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description In vertebrates the most notable feature of the olfactory epithelium (OE) is its ability to continually replace cell types, including mature sensory neurons. In amphibians, the neuronal turnover is observed in adult animals and during larval development also. Today, one of the most used techniques for studies of cell proliferation and differentiation is the 5-bromo-2-deoxyuridine (BrdU) uptake, a synthetic analogue of thymidine that is incorporated into cells during DNA replication. Despite this, and because of its toxicity, some authors have questioned its use for the above mentioned purposes. This work analyses BrdU incorporation technique for cell proliferation and differentiation studies within the OE of amphibian larvae. The methodology used has been validated in Xenopus laevis larvae for studies related to inner ear formation. However, we observe a large number of adverse effects on cellular components of the OE, such as an increase in apoptosis, alterations in the expression pattern of different cell markers, as well as defects in the foraging behavior in animals that were exposed to BrdU. We conclude that even though BrdU can be successfully used as a proliferation cellular marker in the OE, It should be very careful when BrdU is used for studying cell lineage.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182686
Raices, Marilina; Jungblut, Lucas David; Paz, Dante Agustin; Pozzi, Andrea Gabriela; Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium; MedCrave; MOJ Anatomy & Physiology; 1; 4; 11-2015; 1-9
2471-139X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182686
identifier_str_mv Raices, Marilina; Jungblut, Lucas David; Paz, Dante Agustin; Pozzi, Andrea Gabriela; Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium; MedCrave; MOJ Anatomy & Physiology; 1; 4; 11-2015; 1-9
2471-139X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.15406/mojap.2015.01.00023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
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dc.publisher.none.fl_str_mv MedCrave
publisher.none.fl_str_mv MedCrave
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reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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