Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack

Autores
Giacometti, Romina; Barneto, Jesica Alejandra; Barriga, Lucía Guadalupe; Sardoy, Pedro Miguel; Balestrasse, Karina Beatriz; Andrade, Andrea Mariela; Pagano, Eduardo Antonio; Alemano, Sergio Gabriel; Zavala, Jorge Alberto
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Southern green stink bugs (Nezara viridula L.) invade field‐grown soybean crops, where they feed on developing seeds and inject phytotoxic saliva, which causes yield reduction. Although leaf responses to herbivory are well studied, no information is available about the regulation of defences in seeds. RESULTS: This study demonstrated that mitogen‐activated protein kinases MPK3, MPK4 and MPK6 are expressed and activated in developing seeds of field‐grown soybean and regulate a defensive response after stink bug damage. Although 10–20 min after stink bug feeding on seeds induced the expression of MPK3, MPK6 and MPK4, only MPK6 was phosphorylated after damage. Herbivory induced an early peak of jasmonic acid (JA) accumulation and ethylene (ET) emission after 3 h in developing seeds, whereas salicylic acid (SA) was also induced early, and at increasing levels up to 72 h after damage. Damaged seeds upregulated defensive genes typically modulated by JA/ET or SA, which in turn reduced the activity of digestive enzymes in the gut of stink bugs. Induced seeds were less preferred by stink bugs. CONCLUSION: This study shows that stink bug damage induces seed defences, which is perceived early by MPKs that may activate defence metabolic pathways in developing seeds of field‐grown soybean.
Fil: Giacometti, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Barneto, Jesica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Barriga, Lucía Guadalupe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Sardoy, Pedro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Andrade, Andrea Mariela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Pagano, Eduardo Antonio. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Alemano, Sergio Gabriel. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Materia
Herbivory
Jasmonic Acid
Mapk Signalling
Plant Insect Interactions
Salicylic Acid
Glycine Max L
Nezara Viridula L
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46402
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46402
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attackGiacometti, RominaBarneto, Jesica AlejandraBarriga, Lucía GuadalupeSardoy, Pedro MiguelBalestrasse, Karina BeatrizAndrade, Andrea MarielaPagano, Eduardo AntonioAlemano, Sergio GabrielZavala, Jorge AlbertoHerbivoryJasmonic AcidMapk SignallingPlant Insect InteractionsSalicylic AcidGlycine Max LNezara Viridula LBACKGROUND: Southern green stink bugs (Nezara viridula L.) invade field‐grown soybean crops, where they feed on developing seeds and inject phytotoxic saliva, which causes yield reduction. Although leaf responses to herbivory are well studied, no information is available about the regulation of defences in seeds. RESULTS: This study demonstrated that mitogen‐activated protein kinases MPK3, MPK4 and MPK6 are expressed and activated in developing seeds of field‐grown soybean and regulate a defensive response after stink bug damage. Although 10–20 min after stink bug feeding on seeds induced the expression of MPK3, MPK6 and MPK4, only MPK6 was phosphorylated after damage. Herbivory induced an early peak of jasmonic acid (JA) accumulation and ethylene (ET) emission after 3 h in developing seeds, whereas salicylic acid (SA) was also induced early, and at increasing levels up to 72 h after damage. Damaged seeds upregulated defensive genes typically modulated by JA/ET or SA, which in turn reduced the activity of digestive enzymes in the gut of stink bugs. Induced seeds were less preferred by stink bugs. CONCLUSION: This study shows that stink bug damage induces seed defences, which is perceived early by MPKs that may activate defence metabolic pathways in developing seeds of field‐grown soybean.Fil: Giacometti, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Barneto, Jesica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Barriga, Lucía Guadalupe. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Sardoy, Pedro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Andrade, Andrea Mariela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Pagano, Eduardo Antonio. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Alemano, Sergio Gabriel. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaJohn Wiley & Sons Ltd2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46402Giacometti, Romina; Barneto, Jesica Alejandra; Barriga, Lucía Guadalupe; Sardoy, Pedro Miguel; Balestrasse, Karina Beatriz; et al.; Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack; John Wiley & Sons Ltd; Pest Management Science; 11-20151526-498XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4192info:eu-repo/semantics/altIdentifier/doi/10.1002/ps.4192info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:46Zoai:ri.conicet.gov.ar:11336/46402instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:46.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
title Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
spellingShingle Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
Giacometti, Romina
Herbivory
Jasmonic Acid
Mapk Signalling
Plant Insect Interactions
Salicylic Acid
Glycine Max L
Nezara Viridula L
title_short Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
title_full Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
title_fullStr Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
title_full_unstemmed Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
title_sort Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack
dc.creator.none.fl_str_mv Giacometti, Romina
Barneto, Jesica Alejandra
Barriga, Lucía Guadalupe
Sardoy, Pedro Miguel
Balestrasse, Karina Beatriz
Andrade, Andrea Mariela
Pagano, Eduardo Antonio
Alemano, Sergio Gabriel
Zavala, Jorge Alberto
author Giacometti, Romina
author_facet Giacometti, Romina
Barneto, Jesica Alejandra
Barriga, Lucía Guadalupe
Sardoy, Pedro Miguel
Balestrasse, Karina Beatriz
Andrade, Andrea Mariela
Pagano, Eduardo Antonio
Alemano, Sergio Gabriel
Zavala, Jorge Alberto
author_role author
author2 Barneto, Jesica Alejandra
Barriga, Lucía Guadalupe
Sardoy, Pedro Miguel
Balestrasse, Karina Beatriz
Andrade, Andrea Mariela
Pagano, Eduardo Antonio
Alemano, Sergio Gabriel
Zavala, Jorge Alberto
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Herbivory
Jasmonic Acid
Mapk Signalling
Plant Insect Interactions
Salicylic Acid
Glycine Max L
Nezara Viridula L
topic Herbivory
Jasmonic Acid
Mapk Signalling
Plant Insect Interactions
Salicylic Acid
Glycine Max L
Nezara Viridula L
dc.description.none.fl_txt_mv BACKGROUND: Southern green stink bugs (Nezara viridula L.) invade field‐grown soybean crops, where they feed on developing seeds and inject phytotoxic saliva, which causes yield reduction. Although leaf responses to herbivory are well studied, no information is available about the regulation of defences in seeds. RESULTS: This study demonstrated that mitogen‐activated protein kinases MPK3, MPK4 and MPK6 are expressed and activated in developing seeds of field‐grown soybean and regulate a defensive response after stink bug damage. Although 10–20 min after stink bug feeding on seeds induced the expression of MPK3, MPK6 and MPK4, only MPK6 was phosphorylated after damage. Herbivory induced an early peak of jasmonic acid (JA) accumulation and ethylene (ET) emission after 3 h in developing seeds, whereas salicylic acid (SA) was also induced early, and at increasing levels up to 72 h after damage. Damaged seeds upregulated defensive genes typically modulated by JA/ET or SA, which in turn reduced the activity of digestive enzymes in the gut of stink bugs. Induced seeds were less preferred by stink bugs. CONCLUSION: This study shows that stink bug damage induces seed defences, which is perceived early by MPKs that may activate defence metabolic pathways in developing seeds of field‐grown soybean.
Fil: Giacometti, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Barneto, Jesica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Barriga, Lucía Guadalupe. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Sardoy, Pedro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Balestrasse, Karina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Andrade, Andrea Mariela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Pagano, Eduardo Antonio. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Alemano, Sergio Gabriel. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description BACKGROUND: Southern green stink bugs (Nezara viridula L.) invade field‐grown soybean crops, where they feed on developing seeds and inject phytotoxic saliva, which causes yield reduction. Although leaf responses to herbivory are well studied, no information is available about the regulation of defences in seeds. RESULTS: This study demonstrated that mitogen‐activated protein kinases MPK3, MPK4 and MPK6 are expressed and activated in developing seeds of field‐grown soybean and regulate a defensive response after stink bug damage. Although 10–20 min after stink bug feeding on seeds induced the expression of MPK3, MPK6 and MPK4, only MPK6 was phosphorylated after damage. Herbivory induced an early peak of jasmonic acid (JA) accumulation and ethylene (ET) emission after 3 h in developing seeds, whereas salicylic acid (SA) was also induced early, and at increasing levels up to 72 h after damage. Damaged seeds upregulated defensive genes typically modulated by JA/ET or SA, which in turn reduced the activity of digestive enzymes in the gut of stink bugs. Induced seeds were less preferred by stink bugs. CONCLUSION: This study shows that stink bug damage induces seed defences, which is perceived early by MPKs that may activate defence metabolic pathways in developing seeds of field‐grown soybean.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46402
Giacometti, Romina; Barneto, Jesica Alejandra; Barriga, Lucía Guadalupe; Sardoy, Pedro Miguel; Balestrasse, Karina Beatriz; et al.; Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack; John Wiley & Sons Ltd; Pest Management Science; 11-2015
1526-498X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46402
identifier_str_mv Giacometti, Romina; Barneto, Jesica Alejandra; Barriga, Lucía Guadalupe; Sardoy, Pedro Miguel; Balestrasse, Karina Beatriz; et al.; Early perception of stink bug damage in developing seeds of field-grown soybean induces chemical defences and reduces bug attack; John Wiley & Sons Ltd; Pest Management Science; 11-2015
1526-498X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ps.4192
info:eu-repo/semantics/altIdentifier/doi/10.1002/ps.4192
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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