Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
- Autores
- Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; Castellani, Lucas Gabriel; Torres Tejerizo, Gonzalo Arturo; Brom, Susana
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes place
Fil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; México
Fil: Cazares, Daniel. Universidad Nacional Autónoma de México; México
Fil: Rodríguez, Susana. Universidad Nacional Autónoma de México; México
Fil: Cervantes De la Luz, Laura. Universidad Nacional Autónoma de México; México
Fil: Sánchez López, Rosana. Universidad Nacional Autónoma de México; México
Fil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Brom, Susana. Universidad Nacional Autónoma de México; México - Materia
-
PLASMID
ENDOPHYTIC
NODULE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/155640
Ver los metadatos del registro completo
id |
CONICETDig_d6a7c90ad4a6024a85f63c3fd796c0df |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/155640 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodulesBañuelos Vazquez, Luis AlfredoCazares, DanielRodríguez, SusanaCervantes De la Luz, LauraSánchez López, RosanaCastellani, Lucas GabrielTorres Tejerizo, Gonzalo ArturoBrom, SusanaPLASMIDENDOPHYTICNODULEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes placeFil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; MéxicoFil: Cazares, Daniel. Universidad Nacional Autónoma de México; MéxicoFil: Rodríguez, Susana. Universidad Nacional Autónoma de México; MéxicoFil: Cervantes De la Luz, Laura. Universidad Nacional Autónoma de México; MéxicoFil: Sánchez López, Rosana. Universidad Nacional Autónoma de México; MéxicoFil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Brom, Susana. Universidad Nacional Autónoma de México; MéxicoFrontiers Media2020-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155640Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; et al.; Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules; Frontiers Media; Frontiers in Microbiology; 11; 29-7-2020; 1-131664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.01752/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.01752info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:41Zoai:ri.conicet.gov.ar:11336/155640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:42.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
title |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
spellingShingle |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules Bañuelos Vazquez, Luis Alfredo PLASMID ENDOPHYTIC NODULE |
title_short |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
title_full |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
title_fullStr |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
title_full_unstemmed |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
title_sort |
Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules |
dc.creator.none.fl_str_mv |
Bañuelos Vazquez, Luis Alfredo Cazares, Daniel Rodríguez, Susana Cervantes De la Luz, Laura Sánchez López, Rosana Castellani, Lucas Gabriel Torres Tejerizo, Gonzalo Arturo Brom, Susana |
author |
Bañuelos Vazquez, Luis Alfredo |
author_facet |
Bañuelos Vazquez, Luis Alfredo Cazares, Daniel Rodríguez, Susana Cervantes De la Luz, Laura Sánchez López, Rosana Castellani, Lucas Gabriel Torres Tejerizo, Gonzalo Arturo Brom, Susana |
author_role |
author |
author2 |
Cazares, Daniel Rodríguez, Susana Cervantes De la Luz, Laura Sánchez López, Rosana Castellani, Lucas Gabriel Torres Tejerizo, Gonzalo Arturo Brom, Susana |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
PLASMID ENDOPHYTIC NODULE |
topic |
PLASMID ENDOPHYTIC NODULE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes place Fil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; México Fil: Cazares, Daniel. Universidad Nacional Autónoma de México; México Fil: Rodríguez, Susana. Universidad Nacional Autónoma de México; México Fil: Cervantes De la Luz, Laura. Universidad Nacional Autónoma de México; México Fil: Sánchez López, Rosana. Universidad Nacional Autónoma de México; México Fil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Brom, Susana. Universidad Nacional Autónoma de México; México |
description |
Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes place |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-29 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/155640 Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; et al.; Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules; Frontiers Media; Frontiers in Microbiology; 11; 29-7-2020; 1-13 1664-302X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/155640 |
identifier_str_mv |
Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; et al.; Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules; Frontiers Media; Frontiers in Microbiology; 11; 29-7-2020; 1-13 1664-302X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.01752/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.01752 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613315650650112 |
score |
13.070432 |