Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules

Autores
Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; Castellani, Lucas Gabriel; Torres Tejerizo, Gonzalo Arturo; Brom, Susana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes place
Fil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; México
Fil: Cazares, Daniel. Universidad Nacional Autónoma de México; México
Fil: Rodríguez, Susana. Universidad Nacional Autónoma de México; México
Fil: Cervantes De la Luz, Laura. Universidad Nacional Autónoma de México; México
Fil: Sánchez López, Rosana. Universidad Nacional Autónoma de México; México
Fil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Brom, Susana. Universidad Nacional Autónoma de México; México
Materia
PLASMID
ENDOPHYTIC
NODULE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155640

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network_name_str CONICET Digital (CONICET)
spelling Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodulesBañuelos Vazquez, Luis AlfredoCazares, DanielRodríguez, SusanaCervantes De la Luz, LauraSánchez López, RosanaCastellani, Lucas GabrielTorres Tejerizo, Gonzalo ArturoBrom, SusanaPLASMIDENDOPHYTICNODULEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes placeFil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; MéxicoFil: Cazares, Daniel. Universidad Nacional Autónoma de México; MéxicoFil: Rodríguez, Susana. Universidad Nacional Autónoma de México; MéxicoFil: Cervantes De la Luz, Laura. Universidad Nacional Autónoma de México; MéxicoFil: Sánchez López, Rosana. Universidad Nacional Autónoma de México; MéxicoFil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Brom, Susana. Universidad Nacional Autónoma de México; MéxicoFrontiers Media2020-07-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155640Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; et al.; Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules; Frontiers Media; Frontiers in Microbiology; 11; 29-7-2020; 1-131664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.01752/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.01752info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:41Zoai:ri.conicet.gov.ar:11336/155640instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:42.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
title Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
spellingShingle Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
Bañuelos Vazquez, Luis Alfredo
PLASMID
ENDOPHYTIC
NODULE
title_short Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
title_full Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
title_fullStr Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
title_full_unstemmed Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
title_sort Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules
dc.creator.none.fl_str_mv Bañuelos Vazquez, Luis Alfredo
Cazares, Daniel
Rodríguez, Susana
Cervantes De la Luz, Laura
Sánchez López, Rosana
Castellani, Lucas Gabriel
Torres Tejerizo, Gonzalo Arturo
Brom, Susana
author Bañuelos Vazquez, Luis Alfredo
author_facet Bañuelos Vazquez, Luis Alfredo
Cazares, Daniel
Rodríguez, Susana
Cervantes De la Luz, Laura
Sánchez López, Rosana
Castellani, Lucas Gabriel
Torres Tejerizo, Gonzalo Arturo
Brom, Susana
author_role author
author2 Cazares, Daniel
Rodríguez, Susana
Cervantes De la Luz, Laura
Sánchez López, Rosana
Castellani, Lucas Gabriel
Torres Tejerizo, Gonzalo Arturo
Brom, Susana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLASMID
ENDOPHYTIC
NODULE
topic PLASMID
ENDOPHYTIC
NODULE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes place
Fil: Bañuelos Vazquez, Luis Alfredo. Universidad Nacional Autónoma de México; México
Fil: Cazares, Daniel. Universidad Nacional Autónoma de México; México
Fil: Rodríguez, Susana. Universidad Nacional Autónoma de México; México
Fil: Cervantes De la Luz, Laura. Universidad Nacional Autónoma de México; México
Fil: Sánchez López, Rosana. Universidad Nacional Autónoma de México; México
Fil: Castellani, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Brom, Susana. Universidad Nacional Autónoma de México; México
description Conjugative transfer is one of the mechanisms allowing diversification and evolution ofbacteria. Rhizobium etli CFN42 is a bacterial strain whose habitat is the rhizosphere andis able to form nodules as a result of the nitrogen-fixing symbiotic relationship it mayestablish with the roots of Phaseolus vulgaris. R. etli CFN42 contains one chromosomeand six large plasmids (pRet42a ? pRet42f). Most of the genetic information involvedin the establishment of the symbiosis is localized on plasmid pRet42d, named asthe symbiotic plasmid (pSym). This plasmid is able to perform conjugation, usingpSym encoded transfer genes controlled by the RctA/RctB system. Another plasmidof CFN42, pRet42a, has been shown to perform conjugative transfer not only in vitro,but also on the surface of roots and inside nodules, using other rhizobia as recipients.In addition to the rhizobia involved in the formation of nodules, these structures havebeen shown to contain endophytic bacteria from different genera and species. Inthis work, we have explored the conjugative transfer of the pSym (pRet42d) fromR. etli CFN42 to endophytic bacteria as putative recipients, using as donor a CFN42derivative labeled with GFP in the pRet42d and RFP in the chromosome. We wereable to isolate some transconjugants, which inherit the GFP, but not the RFP marker.Some of them were identified, analyzed and evaluated for their ability to nodulate.We found transconjugants from genera such as Stenotrophomonas, Achromobacter,and Bacillus, among others. Although all the transconjugants carried the GFP marker,and nod, fix, and nif genes from pRet42d, not all were able to nodulate. Ultrastructuremicroscopy analysis showed some differences in the structure of the nodules of one ofthe transconjugants. A replicon of the size of pRet42d (371 Kb) could not be visualizedin the transconjugants, suggesting that the pSym or a segment of the plasmid isintegrated in the chromosome of the recipients. These findings strengthen the proposalthat nodules constitute a propitious environment for exchange of genetic informationamong bacteria, in addition to their function as structures where nitrogen fixation andassimilation takes place
publishDate 2020
dc.date.none.fl_str_mv 2020-07-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155640
Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; et al.; Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules; Frontiers Media; Frontiers in Microbiology; 11; 29-7-2020; 1-13
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155640
identifier_str_mv Bañuelos Vazquez, Luis Alfredo; Cazares, Daniel; Rodríguez, Susana; Cervantes De la Luz, Laura; Sánchez López, Rosana; et al.; Transfer of the symbiotic plasmid of rhizobium etli CFN42 to endophytic bacteria inside nodules; Frontiers Media; Frontiers in Microbiology; 11; 29-7-2020; 1-13
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.01752/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.01752
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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