Vicuna oviduct mucosa: Ultrastructure and lectin affinity

Autores
Apichela, Silvana Andrea; Jiménez Díaz, María Atilana; Schuster, Stefanie; Sinowatz, Fred; Miceli, Dora Cristina
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The isthmus and uterotubal junction (UTJ) from oviducts of adult female vicunas (Vicugna vicuna) were used to study the epithelial cell ultrastructure. Scanning microphotographs revealed a mosaic pattern made up of ciliated and secretory cells with abundant microvilli. In the isthmus only a few secretory cells were present, scattered among numerous ciliated cells while in the UTJ the secretory cells prevail. The abundance of secretory cells suggests that the UTJ cells may possess special characteristics for sperm binding. No studies exist in either the vicuna or any of the other South American Camelids (SAC) concerning carbohydrates involved in sperm or embryo interactions on the oviductal surface. Identification of these sugars seems relevant to the mechanism involved in the sperm-oviduct interaction. As a first step in the determination of the molecular mechanism implicated in the formation of sperm reservoirs in the oviduct, lectin affinity for the oviductal mucosa was studied. High concentrations of glycosaminoglycans inside the two types of epithelial cells, and on their glycocalyx were observed with histochemical methods. The carbohydrates on the epithelial surface were labeled with fluorescent by lectins and analyzed with confocal scanning microscopy. The cell surface showed abundant α-mannopyranosyl, α-glucopyranosyl, β-galactosyl, N-acetyl glucosamine, and N-acetylneuraminic acid residues and few α-linked N-acetyl galactosamine residues. Neither α-l-fucopyranosyl nor β-N-acetyl galactosamine residues were observed in any part of the oviduct. The distinct ultrastructural characteristic of the UTJ as well as the presence of high concentrations of sugar residues on the mucosa surface of this portion of the oviduct could be related to its function as a sperm reservoir.
Fil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Jiménez Díaz, María Atilana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Schuster, Stefanie. Ludwig Maximilians Universitat; Alemania
Fil: Sinowatz, Fred. Ludwig Maximilians Universitat; Alemania
Fil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
GLYCOPROTEINS
OVIDUCT
ULTRASTRUCTURE
VICUNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95079
Acceder
id CONICETDig_d64e423eedf58de87179b1155ab178cb
oai_identifier_str oai:ri.conicet.gov.ar:11336/95079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vicuna oviduct mucosa: Ultrastructure and lectin affinityApichela, Silvana AndreaJiménez Díaz, María AtilanaSchuster, StefanieSinowatz, FredMiceli, Dora CristinaGLYCOPROTEINSOVIDUCTULTRASTRUCTUREVICUNAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The isthmus and uterotubal junction (UTJ) from oviducts of adult female vicunas (Vicugna vicuna) were used to study the epithelial cell ultrastructure. Scanning microphotographs revealed a mosaic pattern made up of ciliated and secretory cells with abundant microvilli. In the isthmus only a few secretory cells were present, scattered among numerous ciliated cells while in the UTJ the secretory cells prevail. The abundance of secretory cells suggests that the UTJ cells may possess special characteristics for sperm binding. No studies exist in either the vicuna or any of the other South American Camelids (SAC) concerning carbohydrates involved in sperm or embryo interactions on the oviductal surface. Identification of these sugars seems relevant to the mechanism involved in the sperm-oviduct interaction. As a first step in the determination of the molecular mechanism implicated in the formation of sperm reservoirs in the oviduct, lectin affinity for the oviductal mucosa was studied. High concentrations of glycosaminoglycans inside the two types of epithelial cells, and on their glycocalyx were observed with histochemical methods. The carbohydrates on the epithelial surface were labeled with fluorescent by lectins and analyzed with confocal scanning microscopy. The cell surface showed abundant α-mannopyranosyl, α-glucopyranosyl, β-galactosyl, N-acetyl glucosamine, and N-acetylneuraminic acid residues and few α-linked N-acetyl galactosamine residues. Neither α-l-fucopyranosyl nor β-N-acetyl galactosamine residues were observed in any part of the oviduct. The distinct ultrastructural characteristic of the UTJ as well as the presence of high concentrations of sugar residues on the mucosa surface of this portion of the oviduct could be related to its function as a sperm reservoir.Fil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Jiménez Díaz, María Atilana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Schuster, Stefanie. Ludwig Maximilians Universitat; AlemaniaFil: Sinowatz, Fred. Ludwig Maximilians Universitat; AlemaniaFil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier Science2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95079Apichela, Silvana Andrea; Jiménez Díaz, María Atilana; Schuster, Stefanie; Sinowatz, Fred; Miceli, Dora Cristina; Vicuna oviduct mucosa: Ultrastructure and lectin affinity; Elsevier Science; Journal of Small Ruminant Research; 66; 1-3; 12-2006; 164-1680921-4488CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448805003779info:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2005.09.023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:36:31Zoai:ri.conicet.gov.ar:11336/95079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:36:31.598CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vicuna oviduct mucosa: Ultrastructure and lectin affinity
title Vicuna oviduct mucosa: Ultrastructure and lectin affinity
spellingShingle Vicuna oviduct mucosa: Ultrastructure and lectin affinity
Apichela, Silvana Andrea
GLYCOPROTEINS
OVIDUCT
ULTRASTRUCTURE
VICUNA
title_short Vicuna oviduct mucosa: Ultrastructure and lectin affinity
title_full Vicuna oviduct mucosa: Ultrastructure and lectin affinity
title_fullStr Vicuna oviduct mucosa: Ultrastructure and lectin affinity
title_full_unstemmed Vicuna oviduct mucosa: Ultrastructure and lectin affinity
title_sort Vicuna oviduct mucosa: Ultrastructure and lectin affinity
dc.creator.none.fl_str_mv Apichela, Silvana Andrea
Jiménez Díaz, María Atilana
Schuster, Stefanie
Sinowatz, Fred
Miceli, Dora Cristina
author Apichela, Silvana Andrea
author_facet Apichela, Silvana Andrea
Jiménez Díaz, María Atilana
Schuster, Stefanie
Sinowatz, Fred
Miceli, Dora Cristina
author_role author
author2 Jiménez Díaz, María Atilana
Schuster, Stefanie
Sinowatz, Fred
Miceli, Dora Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv GLYCOPROTEINS
OVIDUCT
ULTRASTRUCTURE
VICUNA
topic GLYCOPROTEINS
OVIDUCT
ULTRASTRUCTURE
VICUNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The isthmus and uterotubal junction (UTJ) from oviducts of adult female vicunas (Vicugna vicuna) were used to study the epithelial cell ultrastructure. Scanning microphotographs revealed a mosaic pattern made up of ciliated and secretory cells with abundant microvilli. In the isthmus only a few secretory cells were present, scattered among numerous ciliated cells while in the UTJ the secretory cells prevail. The abundance of secretory cells suggests that the UTJ cells may possess special characteristics for sperm binding. No studies exist in either the vicuna or any of the other South American Camelids (SAC) concerning carbohydrates involved in sperm or embryo interactions on the oviductal surface. Identification of these sugars seems relevant to the mechanism involved in the sperm-oviduct interaction. As a first step in the determination of the molecular mechanism implicated in the formation of sperm reservoirs in the oviduct, lectin affinity for the oviductal mucosa was studied. High concentrations of glycosaminoglycans inside the two types of epithelial cells, and on their glycocalyx were observed with histochemical methods. The carbohydrates on the epithelial surface were labeled with fluorescent by lectins and analyzed with confocal scanning microscopy. The cell surface showed abundant α-mannopyranosyl, α-glucopyranosyl, β-galactosyl, N-acetyl glucosamine, and N-acetylneuraminic acid residues and few α-linked N-acetyl galactosamine residues. Neither α-l-fucopyranosyl nor β-N-acetyl galactosamine residues were observed in any part of the oviduct. The distinct ultrastructural characteristic of the UTJ as well as the presence of high concentrations of sugar residues on the mucosa surface of this portion of the oviduct could be related to its function as a sperm reservoir.
Fil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Jiménez Díaz, María Atilana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Schuster, Stefanie. Ludwig Maximilians Universitat; Alemania
Fil: Sinowatz, Fred. Ludwig Maximilians Universitat; Alemania
Fil: Miceli, Dora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description The isthmus and uterotubal junction (UTJ) from oviducts of adult female vicunas (Vicugna vicuna) were used to study the epithelial cell ultrastructure. Scanning microphotographs revealed a mosaic pattern made up of ciliated and secretory cells with abundant microvilli. In the isthmus only a few secretory cells were present, scattered among numerous ciliated cells while in the UTJ the secretory cells prevail. The abundance of secretory cells suggests that the UTJ cells may possess special characteristics for sperm binding. No studies exist in either the vicuna or any of the other South American Camelids (SAC) concerning carbohydrates involved in sperm or embryo interactions on the oviductal surface. Identification of these sugars seems relevant to the mechanism involved in the sperm-oviduct interaction. As a first step in the determination of the molecular mechanism implicated in the formation of sperm reservoirs in the oviduct, lectin affinity for the oviductal mucosa was studied. High concentrations of glycosaminoglycans inside the two types of epithelial cells, and on their glycocalyx were observed with histochemical methods. The carbohydrates on the epithelial surface were labeled with fluorescent by lectins and analyzed with confocal scanning microscopy. The cell surface showed abundant α-mannopyranosyl, α-glucopyranosyl, β-galactosyl, N-acetyl glucosamine, and N-acetylneuraminic acid residues and few α-linked N-acetyl galactosamine residues. Neither α-l-fucopyranosyl nor β-N-acetyl galactosamine residues were observed in any part of the oviduct. The distinct ultrastructural characteristic of the UTJ as well as the presence of high concentrations of sugar residues on the mucosa surface of this portion of the oviduct could be related to its function as a sperm reservoir.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95079
Apichela, Silvana Andrea; Jiménez Díaz, María Atilana; Schuster, Stefanie; Sinowatz, Fred; Miceli, Dora Cristina; Vicuna oviduct mucosa: Ultrastructure and lectin affinity; Elsevier Science; Journal of Small Ruminant Research; 66; 1-3; 12-2006; 164-168
0921-4488
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95079
identifier_str_mv Apichela, Silvana Andrea; Jiménez Díaz, María Atilana; Schuster, Stefanie; Sinowatz, Fred; Miceli, Dora Cristina; Vicuna oviduct mucosa: Ultrastructure and lectin affinity; Elsevier Science; Journal of Small Ruminant Research; 66; 1-3; 12-2006; 164-168
0921-4488
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0921448805003779
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.smallrumres.2005.09.023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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