cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon

Autores
Guiu Aragonés, Cèlia; Sánchez Pina, María Amelia; Díaz Pendón, Juan Antonio; Peña, Eduardo José; Heinlein, Manfred; Martín Hernández, Ana Montserrat
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near‐isogenic line SC12‐1‐99, which carries the recessive resistance gene cmv1 from the accession PI 161375, cultivar ‘Songwhan Charmi’. Strains of subgroup I overcome cmv1 resistance in a manner dependent on the movement protein. We characterized the resistance conferred by cmv1 and established that CMV‐LS (subgroup II) can move from cell to cell up to the veins in the inoculated leaf, but cannot enter the phloem. Immunogold labelling at transmission electron microscopy level showed that CMV‐LS remains restricted to the bundle sheath (BS) cells in the resistant line, and does not invade vascular parenchyma or intermediary cells, whereas, in the susceptible line ‘Piel de Sapo’ (PS), the virus invades all vein cell types. These observations indicate that the resistant allele of cmv1 restricts systemic infection in a virus strain‐ and cell type‐specific manner by acting as an important gatekeeper for virus progression from BS cells to phloem cells. Graft inoculation experiments showed that CMV‐LS cannot move from the infected PS stock into the resistant cmv1 scion, thus suggesting an additional role for cmv1 related to CMV transport within or exit from the phloem. The characterization of this new form of recessive resistance, based on a restriction of virus systemic movement, opens up the possibility to design alternative approaches for breeding strategies in melon.
Fil: Guiu Aragonés, Cèlia. Consejo Superior de Investigaciones Científicas; España. Institut de Recerca i Tecnologia Agroalimentàries; España. Universitat Autònoma de Barcelona; España
Fil: Sánchez Pina, María Amelia. Consejo Superior de Investigaciones Científicas; España. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Díaz Pendón, Juan Antonio. Consejo Superior de Investigaciones Científicas; España. Universidad de Málaga; España
Fil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
Fil: Heinlein, Manfred. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
Fil: Martín Hernández, Ana Montserrat. Consejo Superior de Investigaciones Científicas; España. Institut de Recerca i Tecnologia Agroalimentàries; España. Universitat Autònoma de Barcelona; España
Materia
CUCUMBER MOSAIC VIRUS
RECESSIVE RESISTANCE
CMV1
PHLOEM ENTRY
SYSTEMIC INFECTION
VIRUS MOVEMENT
HOST FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47889
Acceder
id CONICETDig_d63c694b2d66bf4d9710e4c66445b899
oai_identifier_str oai:ri.conicet.gov.ar:11336/47889
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melonGuiu Aragonés, CèliaSánchez Pina, María AmeliaDíaz Pendón, Juan AntonioPeña, Eduardo JoséHeinlein, ManfredMartín Hernández, Ana MontserratCUCUMBER MOSAIC VIRUSRECESSIVE RESISTANCECMV1PHLOEM ENTRYSYSTEMIC INFECTIONVIRUS MOVEMENTHOST FACTORhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near‐isogenic line SC12‐1‐99, which carries the recessive resistance gene cmv1 from the accession PI 161375, cultivar ‘Songwhan Charmi’. Strains of subgroup I overcome cmv1 resistance in a manner dependent on the movement protein. We characterized the resistance conferred by cmv1 and established that CMV‐LS (subgroup II) can move from cell to cell up to the veins in the inoculated leaf, but cannot enter the phloem. Immunogold labelling at transmission electron microscopy level showed that CMV‐LS remains restricted to the bundle sheath (BS) cells in the resistant line, and does not invade vascular parenchyma or intermediary cells, whereas, in the susceptible line ‘Piel de Sapo’ (PS), the virus invades all vein cell types. These observations indicate that the resistant allele of cmv1 restricts systemic infection in a virus strain‐ and cell type‐specific manner by acting as an important gatekeeper for virus progression from BS cells to phloem cells. Graft inoculation experiments showed that CMV‐LS cannot move from the infected PS stock into the resistant cmv1 scion, thus suggesting an additional role for cmv1 related to CMV transport within or exit from the phloem. The characterization of this new form of recessive resistance, based on a restriction of virus systemic movement, opens up the possibility to design alternative approaches for breeding strategies in melon.Fil: Guiu Aragonés, Cèlia. Consejo Superior de Investigaciones Científicas; España. Institut de Recerca i Tecnologia Agroalimentàries; España. Universitat Autònoma de Barcelona; EspañaFil: Sánchez Pina, María Amelia. Consejo Superior de Investigaciones Científicas; España. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Díaz Pendón, Juan Antonio. Consejo Superior de Investigaciones Científicas; España. Universidad de Málaga; EspañaFil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; FranciaFil: Heinlein, Manfred. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; FranciaFil: Martín Hernández, Ana Montserrat. Consejo Superior de Investigaciones Científicas; España. Institut de Recerca i Tecnologia Agroalimentàries; España. Universitat Autònoma de Barcelona; EspañaWiley Blackwell Publishing, Inc2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47889Guiu Aragonés, Cèlia; Sánchez Pina, María Amelia; Díaz Pendón, Juan Antonio; Peña, Eduardo José; Heinlein, Manfred; et al.; cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 6; 8-2016; 973-9841464-6722CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/mpp.12351info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12351info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:19Zoai:ri.conicet.gov.ar:11336/47889instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:19.48CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
spellingShingle cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
Guiu Aragonés, Cèlia
CUCUMBER MOSAIC VIRUS
RECESSIVE RESISTANCE
CMV1
PHLOEM ENTRY
SYSTEMIC INFECTION
VIRUS MOVEMENT
HOST FACTOR
title_short cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_full cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_fullStr cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_full_unstemmed cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
title_sort cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon
dc.creator.none.fl_str_mv Guiu Aragonés, Cèlia
Sánchez Pina, María Amelia
Díaz Pendón, Juan Antonio
Peña, Eduardo José
Heinlein, Manfred
Martín Hernández, Ana Montserrat
author Guiu Aragonés, Cèlia
author_facet Guiu Aragonés, Cèlia
Sánchez Pina, María Amelia
Díaz Pendón, Juan Antonio
Peña, Eduardo José
Heinlein, Manfred
Martín Hernández, Ana Montserrat
author_role author
author2 Sánchez Pina, María Amelia
Díaz Pendón, Juan Antonio
Peña, Eduardo José
Heinlein, Manfred
Martín Hernández, Ana Montserrat
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CUCUMBER MOSAIC VIRUS
RECESSIVE RESISTANCE
CMV1
PHLOEM ENTRY
SYSTEMIC INFECTION
VIRUS MOVEMENT
HOST FACTOR
topic CUCUMBER MOSAIC VIRUS
RECESSIVE RESISTANCE
CMV1
PHLOEM ENTRY
SYSTEMIC INFECTION
VIRUS MOVEMENT
HOST FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near‐isogenic line SC12‐1‐99, which carries the recessive resistance gene cmv1 from the accession PI 161375, cultivar ‘Songwhan Charmi’. Strains of subgroup I overcome cmv1 resistance in a manner dependent on the movement protein. We characterized the resistance conferred by cmv1 and established that CMV‐LS (subgroup II) can move from cell to cell up to the veins in the inoculated leaf, but cannot enter the phloem. Immunogold labelling at transmission electron microscopy level showed that CMV‐LS remains restricted to the bundle sheath (BS) cells in the resistant line, and does not invade vascular parenchyma or intermediary cells, whereas, in the susceptible line ‘Piel de Sapo’ (PS), the virus invades all vein cell types. These observations indicate that the resistant allele of cmv1 restricts systemic infection in a virus strain‐ and cell type‐specific manner by acting as an important gatekeeper for virus progression from BS cells to phloem cells. Graft inoculation experiments showed that CMV‐LS cannot move from the infected PS stock into the resistant cmv1 scion, thus suggesting an additional role for cmv1 related to CMV transport within or exit from the phloem. The characterization of this new form of recessive resistance, based on a restriction of virus systemic movement, opens up the possibility to design alternative approaches for breeding strategies in melon.
Fil: Guiu Aragonés, Cèlia. Consejo Superior de Investigaciones Científicas; España. Institut de Recerca i Tecnologia Agroalimentàries; España. Universitat Autònoma de Barcelona; España
Fil: Sánchez Pina, María Amelia. Consejo Superior de Investigaciones Científicas; España. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Díaz Pendón, Juan Antonio. Consejo Superior de Investigaciones Científicas; España. Universidad de Málaga; España
Fil: Peña, Eduardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
Fil: Heinlein, Manfred. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
Fil: Martín Hernández, Ana Montserrat. Consejo Superior de Investigaciones Científicas; España. Institut de Recerca i Tecnologia Agroalimentàries; España. Universitat Autònoma de Barcelona; España
description Cucumber mosaic virus (CMV) has the broadest host range among plant viruses, causing enormous losses in agriculture. In melon, strains of subgroup II are unable to establish a systemic infection in the near‐isogenic line SC12‐1‐99, which carries the recessive resistance gene cmv1 from the accession PI 161375, cultivar ‘Songwhan Charmi’. Strains of subgroup I overcome cmv1 resistance in a manner dependent on the movement protein. We characterized the resistance conferred by cmv1 and established that CMV‐LS (subgroup II) can move from cell to cell up to the veins in the inoculated leaf, but cannot enter the phloem. Immunogold labelling at transmission electron microscopy level showed that CMV‐LS remains restricted to the bundle sheath (BS) cells in the resistant line, and does not invade vascular parenchyma or intermediary cells, whereas, in the susceptible line ‘Piel de Sapo’ (PS), the virus invades all vein cell types. These observations indicate that the resistant allele of cmv1 restricts systemic infection in a virus strain‐ and cell type‐specific manner by acting as an important gatekeeper for virus progression from BS cells to phloem cells. Graft inoculation experiments showed that CMV‐LS cannot move from the infected PS stock into the resistant cmv1 scion, thus suggesting an additional role for cmv1 related to CMV transport within or exit from the phloem. The characterization of this new form of recessive resistance, based on a restriction of virus systemic movement, opens up the possibility to design alternative approaches for breeding strategies in melon.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47889
Guiu Aragonés, Cèlia; Sánchez Pina, María Amelia; Díaz Pendón, Juan Antonio; Peña, Eduardo José; Heinlein, Manfred; et al.; cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 6; 8-2016; 973-984
1464-6722
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47889
identifier_str_mv Guiu Aragonés, Cèlia; Sánchez Pina, María Amelia; Díaz Pendón, Juan Antonio; Peña, Eduardo José; Heinlein, Manfred; et al.; cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 6; 8-2016; 973-984
1464-6722
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/mpp.12351
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12351
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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