Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants

Autores
Biruk, Lucia Nadia; Moretton, Juan; Fabrizio de Iorio, Alicia Rosa; Weigandt, Cristian; Etcheverry, Jimena; Filippetto, Javier; Magdaleno, Anahí
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to investigate the parameters of chemical extraction associated with the detection of toxicity and genotoxicity in sediment sample extracts. Quantitative analysis of metals and polycyclic aromatic hydrocarbons (PAHs), together with a battery of four bioassays, was performed in order to evaluate the extraction efficiency of inorganic and organic toxicants. The extracts were carried out using two inorganic solvents, two organic solvents and two extraction methodologies, making a total of five extracts. Two toxicity tests, the algal growth inhibition of Pseudokirchneriella subcapitata and the root elongation inhibition of Lactuca sativa, and two genotoxicity tests, the analysis of revertants of Salmonella typhimurium and the analysis of micronuclei and chromosomal aberrations in Allium cepa, were performed. According to the chemical analysis, the acidic solution extracted more heavy metal concentrations than distilled water, and dichloromethane extracted more but fewer concentrations of PAH compounds than methanol. Shaker extracts with distilled water were non-toxic to P. subcapitata, but were toxic to L. sativa. The acidic extracts were more toxic to P. subcapitata than to L. sativa. The methanolic organic extracts were more toxic to the alga than those obtained with dichloromethane. None of these extracts resulted toxic to L. sativa. Mutagenic effects were only detected in the organic dichloromethane extracts in the presence of metabolic activation. All the inorganic and organic extracts were genotoxic to A. cepa. This study showed that the implementation of different extraction methods together with a battery of bioassays could be suitable tools for detecting toxicity and genotoxicity in sediment samples.
Fil: Biruk, Lucia Nadia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moretton, Juan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Fabrizio de Iorio, Alicia Rosa. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Weigandt, Cristian. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Etcheverry, Jimena. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Filippetto, Javier. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Magdaleno, Anahí. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Battery of Bioassays
Genotoxicity
Sediments
Toxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45394
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45394
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminantsBiruk, Lucia NadiaMoretton, JuanFabrizio de Iorio, Alicia RosaWeigandt, CristianEtcheverry, JimenaFilippetto, JavierMagdaleno, AnahíBattery of BioassaysGenotoxicitySedimentsToxicityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to investigate the parameters of chemical extraction associated with the detection of toxicity and genotoxicity in sediment sample extracts. Quantitative analysis of metals and polycyclic aromatic hydrocarbons (PAHs), together with a battery of four bioassays, was performed in order to evaluate the extraction efficiency of inorganic and organic toxicants. The extracts were carried out using two inorganic solvents, two organic solvents and two extraction methodologies, making a total of five extracts. Two toxicity tests, the algal growth inhibition of Pseudokirchneriella subcapitata and the root elongation inhibition of Lactuca sativa, and two genotoxicity tests, the analysis of revertants of Salmonella typhimurium and the analysis of micronuclei and chromosomal aberrations in Allium cepa, were performed. According to the chemical analysis, the acidic solution extracted more heavy metal concentrations than distilled water, and dichloromethane extracted more but fewer concentrations of PAH compounds than methanol. Shaker extracts with distilled water were non-toxic to P. subcapitata, but were toxic to L. sativa. The acidic extracts were more toxic to P. subcapitata than to L. sativa. The methanolic organic extracts were more toxic to the alga than those obtained with dichloromethane. None of these extracts resulted toxic to L. sativa. Mutagenic effects were only detected in the organic dichloromethane extracts in the presence of metabolic activation. All the inorganic and organic extracts were genotoxic to A. cepa. This study showed that the implementation of different extraction methods together with a battery of bioassays could be suitable tools for detecting toxicity and genotoxicity in sediment samples.Fil: Biruk, Lucia Nadia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moretton, Juan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Fabrizio de Iorio, Alicia Rosa. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Weigandt, Cristian. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Etcheverry, Jimena. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Filippetto, Javier. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Magdaleno, Anahí. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAcademic Press Inc Elsevier Science2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45394Biruk, Lucia Nadia; Moretton, Juan; Fabrizio de Iorio, Alicia Rosa; Weigandt, Cristian; Etcheverry, Jimena; et al.; Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 135; 1-2017; 302-3110147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2016.09.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:25:28Zoai:ri.conicet.gov.ar:11336/45394instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:25:29.22CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
title Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
spellingShingle Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
Biruk, Lucia Nadia
Battery of Bioassays
Genotoxicity
Sediments
Toxicity
title_short Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
title_full Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
title_fullStr Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
title_full_unstemmed Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
title_sort Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants
dc.creator.none.fl_str_mv Biruk, Lucia Nadia
Moretton, Juan
Fabrizio de Iorio, Alicia Rosa
Weigandt, Cristian
Etcheverry, Jimena
Filippetto, Javier
Magdaleno, Anahí
author Biruk, Lucia Nadia
author_facet Biruk, Lucia Nadia
Moretton, Juan
Fabrizio de Iorio, Alicia Rosa
Weigandt, Cristian
Etcheverry, Jimena
Filippetto, Javier
Magdaleno, Anahí
author_role author
author2 Moretton, Juan
Fabrizio de Iorio, Alicia Rosa
Weigandt, Cristian
Etcheverry, Jimena
Filippetto, Javier
Magdaleno, Anahí
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Battery of Bioassays
Genotoxicity
Sediments
Toxicity
topic Battery of Bioassays
Genotoxicity
Sediments
Toxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to investigate the parameters of chemical extraction associated with the detection of toxicity and genotoxicity in sediment sample extracts. Quantitative analysis of metals and polycyclic aromatic hydrocarbons (PAHs), together with a battery of four bioassays, was performed in order to evaluate the extraction efficiency of inorganic and organic toxicants. The extracts were carried out using two inorganic solvents, two organic solvents and two extraction methodologies, making a total of five extracts. Two toxicity tests, the algal growth inhibition of Pseudokirchneriella subcapitata and the root elongation inhibition of Lactuca sativa, and two genotoxicity tests, the analysis of revertants of Salmonella typhimurium and the analysis of micronuclei and chromosomal aberrations in Allium cepa, were performed. According to the chemical analysis, the acidic solution extracted more heavy metal concentrations than distilled water, and dichloromethane extracted more but fewer concentrations of PAH compounds than methanol. Shaker extracts with distilled water were non-toxic to P. subcapitata, but were toxic to L. sativa. The acidic extracts were more toxic to P. subcapitata than to L. sativa. The methanolic organic extracts were more toxic to the alga than those obtained with dichloromethane. None of these extracts resulted toxic to L. sativa. Mutagenic effects were only detected in the organic dichloromethane extracts in the presence of metabolic activation. All the inorganic and organic extracts were genotoxic to A. cepa. This study showed that the implementation of different extraction methods together with a battery of bioassays could be suitable tools for detecting toxicity and genotoxicity in sediment samples.
Fil: Biruk, Lucia Nadia. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moretton, Juan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Fabrizio de Iorio, Alicia Rosa. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Weigandt, Cristian. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Etcheverry, Jimena. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Filippetto, Javier. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Magdaleno, Anahí. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this study was to investigate the parameters of chemical extraction associated with the detection of toxicity and genotoxicity in sediment sample extracts. Quantitative analysis of metals and polycyclic aromatic hydrocarbons (PAHs), together with a battery of four bioassays, was performed in order to evaluate the extraction efficiency of inorganic and organic toxicants. The extracts were carried out using two inorganic solvents, two organic solvents and two extraction methodologies, making a total of five extracts. Two toxicity tests, the algal growth inhibition of Pseudokirchneriella subcapitata and the root elongation inhibition of Lactuca sativa, and two genotoxicity tests, the analysis of revertants of Salmonella typhimurium and the analysis of micronuclei and chromosomal aberrations in Allium cepa, were performed. According to the chemical analysis, the acidic solution extracted more heavy metal concentrations than distilled water, and dichloromethane extracted more but fewer concentrations of PAH compounds than methanol. Shaker extracts with distilled water were non-toxic to P. subcapitata, but were toxic to L. sativa. The acidic extracts were more toxic to P. subcapitata than to L. sativa. The methanolic organic extracts were more toxic to the alga than those obtained with dichloromethane. None of these extracts resulted toxic to L. sativa. Mutagenic effects were only detected in the organic dichloromethane extracts in the presence of metabolic activation. All the inorganic and organic extracts were genotoxic to A. cepa. This study showed that the implementation of different extraction methods together with a battery of bioassays could be suitable tools for detecting toxicity and genotoxicity in sediment samples.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45394
Biruk, Lucia Nadia; Moretton, Juan; Fabrizio de Iorio, Alicia Rosa; Weigandt, Cristian; Etcheverry, Jimena; et al.; Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 135; 1-2017; 302-311
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45394
identifier_str_mv Biruk, Lucia Nadia; Moretton, Juan; Fabrizio de Iorio, Alicia Rosa; Weigandt, Cristian; Etcheverry, Jimena; et al.; Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 135; 1-2017; 302-311
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2016.09.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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