Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea

Autores
Lipke, Elisabeth; Ramirez, Martin Javier; Michalik, Peter
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Haplogynae are highly diverse with respect to the primary male genital system and sperm characteristics. Additionally, all sperm transfer forms (STF) known for spiders are present. Besides individually transferred sperm (cleistospermia), sperm are transferred as conjugates, both primary (synspermia) and secondary sperm conjugates (coenospermia, rouleaux) occur. Nevertheless, the ultrastructure of spermatozoa and STF are described for few Haplogynae and often only one representative species was studied, resulting in a superficial insight in the evolution of these traits. To elucidate the evolution of STF within Haplogynae we investigated representatives of four genera of the dysderoid family Orsolobidae. Our data show the presence of synspermia (Orsolobus, Osornolobus, Hickmanolobus, and Tasmanoonops) and also cleistospermia (Osornolobus). The occurrence of different STF within one family or even genus has not been described for any other spider taxon so far. Moreover, the synspermia of species of Tasmanoonops and Hickmanolobus were not covered by a secretion sheath suggesting a previously unknown strategy of transferring sperm that is possibly related to sperm residency time or female triggered processes after copulation. Based on serial ultrathin sectioning and subsequent 3D-reconstruction, we obtained detailed measurements revealing remarkable size differences of STF. To evaluate the previously suggested correlation with the most distal region of the spermophor inside the embolus (intromittent part of the copulatory organ) we measured the diameter of the spermophor using micro-computed X-ray tomography data to obtain corresponding morphometric parameters. Based on these data only two species show similarity in STF and spermophor diameter.
Fil: Lipke, Elisabeth. Ernst-Moritz-Arndt University of Greifswald; Alemania
Fil: Ramirez, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Michalik, Peter. Ernst-Moritz-Arndt University of Greifswald; Alemania. American Museum Of Natural History; Estados Unidos
Materia
3d Reconstruction
Micro-Computer X-Ray Tomography
Palpal Organ
Cleitospermia
Synspermia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19233
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19233
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in DysderoideaLipke, ElisabethRamirez, Martin JavierMichalik, Peter3d ReconstructionMicro-Computer X-Ray TomographyPalpal OrganCleitospermiaSynspermiahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Haplogynae are highly diverse with respect to the primary male genital system and sperm characteristics. Additionally, all sperm transfer forms (STF) known for spiders are present. Besides individually transferred sperm (cleistospermia), sperm are transferred as conjugates, both primary (synspermia) and secondary sperm conjugates (coenospermia, rouleaux) occur. Nevertheless, the ultrastructure of spermatozoa and STF are described for few Haplogynae and often only one representative species was studied, resulting in a superficial insight in the evolution of these traits. To elucidate the evolution of STF within Haplogynae we investigated representatives of four genera of the dysderoid family Orsolobidae. Our data show the presence of synspermia (Orsolobus, Osornolobus, Hickmanolobus, and Tasmanoonops) and also cleistospermia (Osornolobus). The occurrence of different STF within one family or even genus has not been described for any other spider taxon so far. Moreover, the synspermia of species of Tasmanoonops and Hickmanolobus were not covered by a secretion sheath suggesting a previously unknown strategy of transferring sperm that is possibly related to sperm residency time or female triggered processes after copulation. Based on serial ultrathin sectioning and subsequent 3D-reconstruction, we obtained detailed measurements revealing remarkable size differences of STF. To evaluate the previously suggested correlation with the most distal region of the spermophor inside the embolus (intromittent part of the copulatory organ) we measured the diameter of the spermophor using micro-computed X-ray tomography data to obtain corresponding morphometric parameters. Based on these data only two species show similarity in STF and spermophor diameter.Fil: Lipke, Elisabeth. Ernst-Moritz-Arndt University of Greifswald; AlemaniaFil: Ramirez, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Michalik, Peter. Ernst-Moritz-Arndt University of Greifswald; Alemania. American Museum Of Natural History; Estados UnidosWiley2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19233Lipke, Elisabeth; Ramirez, Martin Javier; Michalik, Peter; Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea; Wiley; Journal of Morphology; 275; 11; 11-2014; 1238-12570362-2525CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jmor.20298info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jmor.20298/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:34Zoai:ri.conicet.gov.ar:11336/19233instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:35.034CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
title Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
spellingShingle Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
Lipke, Elisabeth
3d Reconstruction
Micro-Computer X-Ray Tomography
Palpal Organ
Cleitospermia
Synspermia
title_short Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
title_full Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
title_fullStr Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
title_full_unstemmed Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
title_sort Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea
dc.creator.none.fl_str_mv Lipke, Elisabeth
Ramirez, Martin Javier
Michalik, Peter
author Lipke, Elisabeth
author_facet Lipke, Elisabeth
Ramirez, Martin Javier
Michalik, Peter
author_role author
author2 Ramirez, Martin Javier
Michalik, Peter
author2_role author
author
dc.subject.none.fl_str_mv 3d Reconstruction
Micro-Computer X-Ray Tomography
Palpal Organ
Cleitospermia
Synspermia
topic 3d Reconstruction
Micro-Computer X-Ray Tomography
Palpal Organ
Cleitospermia
Synspermia
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Haplogynae are highly diverse with respect to the primary male genital system and sperm characteristics. Additionally, all sperm transfer forms (STF) known for spiders are present. Besides individually transferred sperm (cleistospermia), sperm are transferred as conjugates, both primary (synspermia) and secondary sperm conjugates (coenospermia, rouleaux) occur. Nevertheless, the ultrastructure of spermatozoa and STF are described for few Haplogynae and often only one representative species was studied, resulting in a superficial insight in the evolution of these traits. To elucidate the evolution of STF within Haplogynae we investigated representatives of four genera of the dysderoid family Orsolobidae. Our data show the presence of synspermia (Orsolobus, Osornolobus, Hickmanolobus, and Tasmanoonops) and also cleistospermia (Osornolobus). The occurrence of different STF within one family or even genus has not been described for any other spider taxon so far. Moreover, the synspermia of species of Tasmanoonops and Hickmanolobus were not covered by a secretion sheath suggesting a previously unknown strategy of transferring sperm that is possibly related to sperm residency time or female triggered processes after copulation. Based on serial ultrathin sectioning and subsequent 3D-reconstruction, we obtained detailed measurements revealing remarkable size differences of STF. To evaluate the previously suggested correlation with the most distal region of the spermophor inside the embolus (intromittent part of the copulatory organ) we measured the diameter of the spermophor using micro-computed X-ray tomography data to obtain corresponding morphometric parameters. Based on these data only two species show similarity in STF and spermophor diameter.
Fil: Lipke, Elisabeth. Ernst-Moritz-Arndt University of Greifswald; Alemania
Fil: Ramirez, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentina
Fil: Michalik, Peter. Ernst-Moritz-Arndt University of Greifswald; Alemania. American Museum Of Natural History; Estados Unidos
description Haplogynae are highly diverse with respect to the primary male genital system and sperm characteristics. Additionally, all sperm transfer forms (STF) known for spiders are present. Besides individually transferred sperm (cleistospermia), sperm are transferred as conjugates, both primary (synspermia) and secondary sperm conjugates (coenospermia, rouleaux) occur. Nevertheless, the ultrastructure of spermatozoa and STF are described for few Haplogynae and often only one representative species was studied, resulting in a superficial insight in the evolution of these traits. To elucidate the evolution of STF within Haplogynae we investigated representatives of four genera of the dysderoid family Orsolobidae. Our data show the presence of synspermia (Orsolobus, Osornolobus, Hickmanolobus, and Tasmanoonops) and also cleistospermia (Osornolobus). The occurrence of different STF within one family or even genus has not been described for any other spider taxon so far. Moreover, the synspermia of species of Tasmanoonops and Hickmanolobus were not covered by a secretion sheath suggesting a previously unknown strategy of transferring sperm that is possibly related to sperm residency time or female triggered processes after copulation. Based on serial ultrathin sectioning and subsequent 3D-reconstruction, we obtained detailed measurements revealing remarkable size differences of STF. To evaluate the previously suggested correlation with the most distal region of the spermophor inside the embolus (intromittent part of the copulatory organ) we measured the diameter of the spermophor using micro-computed X-ray tomography data to obtain corresponding morphometric parameters. Based on these data only two species show similarity in STF and spermophor diameter.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19233
Lipke, Elisabeth; Ramirez, Martin Javier; Michalik, Peter; Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea; Wiley; Journal of Morphology; 275; 11; 11-2014; 1238-1257
0362-2525
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19233
identifier_str_mv Lipke, Elisabeth; Ramirez, Martin Javier; Michalik, Peter; Ultrastructure of spermatozoa of orsolobidae (Haplogynae, Araneae) with implications on the evolution of sperm transfer forms in Dysderoidea; Wiley; Journal of Morphology; 275; 11; 11-2014; 1238-1257
0362-2525
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jmor.20298
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jmor.20298/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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