Fatty acid nitroalkenes ameliorate glucose intolerance and pulmonary hypertension in high-fat diet-induced obesity

Authors
Kelley, Eric E.; Baust, Jeff; Bonacci, Gustavo Roberto; Golin Bisello, Franca; Devlin, Jason E.; Croix, Claudette M. St.; Watkins, Simon C.; Gor, Sonia; Cantu Medellin, Nadiezhda; Weidert, Eric R.; Frisbee,Jefferson C.; Gladwin, Mark T.; Champion, Hunter C.; Freeman, Bruce A.; Khoo, Nicholas K.H.
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Aims Obesity is a risk factor for diabetes and cardiovascular diseases, with the incidence of these disorders becoming epidemic. Pathogenic responses to obesity have been ascribed to adipose tissue (AT) dysfunction that promotes bioactive mediator secretion from visceral AT and the initiation of pro-inflammatory events that induce oxidative stress and tissue dysfunction. Current understanding supports that suppressing pro-inflammatory and oxidative events promotes improved metabolic and cardiovascular function. In this regard, electrophilic nitro-fatty acids display pleiotropic anti-inflammatory signalling actions. Methods and results It was hypothesized that high-fat diet (HFD)-induced inflammatory and metabolic responses, manifested by loss of glucose tolerance and vascular dysfunction, would be attenuated by systemic administration of nitrooctadecenoic acid (OA-NO2). Male C57BL/6j mice subjected to a HFD for 20 weeks displayed increased adiposity, fasting glucose, and insulin levels, which led to glucose intolerance and pulmonary hypertension, characterized by increased right ventricular (RV) end-systolic pressure (RVESP) and pulmonary vascular resistance (PVR). This was associated with increased lung xanthine oxidoreductase (XO) activity, macrophage infiltration, and enhanced expression of pro-inflammatory cytokines. Left ventricular (LV) end-diastolic pressure remained unaltered, indicating that the HFD produces pulmonary vascular remodelling, rather than LV dysfunction and pulmonary venous hypertension. Administration of OA-NO2 for the final 6.5 weeks of HFD improved glucose tolerance and significantly attenuated HFD-induced RVESP, PVR, RV hypertrophy, lung XO activity, oxidative stress, and pro-inflammatory pulmonary cytokine levels. Conclusions These observations support that the pleiotropic signalling actions of electrophilic fatty acids represent a therapeutic strategy for limiting the complex pathogenic responses instigated by obesity.
Fil: Kelley, Eric E.. University of Pittsburgh; Estados Unidos
Fil: Baust, Jeff. University of Pittsburgh; Estados Unidos
Fil: Bonacci, Gustavo Roberto. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Golin Bisello, Franca. University of Pittsburgh; Estados Unidos
Fil: Devlin, Jason E.. University of Pittsburgh; Estados Unidos
Fil: Croix, Claudette M. St.. University of Pittsburgh; Estados Unidos
Fil: Watkins, Simon C.. University of Pittsburgh; Estados Unidos
Fil: Gor, Sonia. University of Pittsburgh; Estados Unidos
Fil: Cantu Medellin, Nadiezhda. University of Pittsburgh; Estados Unidos
Fil: Weidert, Eric R.. University of Pittsburgh; Estados Unidos
Fil: Frisbee,Jefferson C.. University of Virginia; Estados Unidos
Fil: Gladwin, Mark T.. University of Pittsburgh; Estados Unidos
Fil: Champion, Hunter C.. University of Pittsburgh; Estados Unidos
Fil: Freeman, Bruce A.. University of Pittsburgh; Estados Unidos
Fil: Khoo, Nicholas K.H.. University of Pittsburgh; Estados Unidos
Subject
Obesity
Pulmonary hypertension
Nitro-fatty acid signalling
Inflammation
Xanthine Oxidase
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Open access
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/31954